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The map label for this gene is rfbF [H]

Identifier: 74317899

GI number: 74317899

Start: 1978510

End: 1979268

Strand: Reverse

Name: rfbF [H]

Synonym: Tbd_1881

Alternate gene names: 74317899

Gene position: 1979268-1978510 (Counterclockwise)

Preceding gene: 74317900

Following gene: 74317898

Centisome position: 68.02

GC content: 59.29

Gene sequence:

>759_bases
GTGATATTGGCAGGCGGGCTGGGAACCCGTTTAAGCGAAGAAACGGGCGTGCGCCCCAAGCCTATGGTCGAGGTCGGCGG
CAAGCCGATCCTATGGCACATCATGAAGCTGTATTCGCACTATGGGATCGATGAATTCATCGTCTGCCTAGGCTACAAAG
GCTACATGATCAAGGAGTATTTCGCGAACTACTTTCTCCATATGTCGGACATCACTTTCGACATGTCCAGGAACGAGATG
CAGGTGCATCAGAACAACGCGGAGCCGTGGCGGGTGACCTTGGTGGACACGGGGGACGTCACCCAAACGGGAGGGCGGCT
CCGGCGCGTCCGGGAATATCTGGGGGACGACGATTTCTGCATGACCTACGGCGATGGCGTCGGCGATGTGGAGATCGACA
AGCTGATGGCGTTTCATCGCGCGCACGGCTGCCTCGCTACGGTGACTGCCACGCAGCCTCCGGGCCGGTTCGGCGCGCTC
GATCTCGATGGCAGCAGCGTAATGAGCTTTTGCGAGAAGCCGCATGGTGACGGCAGTTGGATCAACGGCGGCTTCTTCGT
GCTGTCGCCGAAGGTGATCGACTATATCAGCGGCGATTCGACGCTCTGGGAGCGGGAGCCGCTCGAAAGGCTGGCGAGCG
AGAAGCAGCTCCGGGCTTATTTTCATCACGGCTTCTGGCAGCCGATGGACACCCTGCGCGACAAGCAACATTTGGAATCG
CTTTGGCAGGAGGGCAAGGCGCCGTGGAAGATATGGTGA

Upstream 100 bases:

>100_bases
GCCGAGGATGCCTACCTTGTTATCGATCGCGAACGTATAGCGCAGGCGGGTGCTTGATTCCGGAAAATGTTGATTTACGG
ACGAGGTGAAAGTGAAAGCA

Downstream 100 bases:

>100_bases
CGAGCCCGGACTTCTGGCAGGGCAAGCGGGTTTTTGTAACGGGTCACACGGGCTTCAAGGGGAGCTGGCTCTGTCTTTGG
CTGCAAAGCATGGGGGCGGA

Product: alpha-D-glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 252; Mature: 252

Protein sequence:

>252_residues
MILAGGLGTRLSEETGVRPKPMVEVGGKPILWHIMKLYSHYGIDEFIVCLGYKGYMIKEYFANYFLHMSDITFDMSRNEM
QVHQNNAEPWRVTLVDTGDVTQTGGRLRRVREYLGDDDFCMTYGDGVGDVEIDKLMAFHRAHGCLATVTATQPPGRFGAL
DLDGSSVMSFCEKPHGDGSWINGGFFVLSPKVIDYISGDSTLWEREPLERLASEKQLRAYFHHGFWQPMDTLRDKQHLES
LWQEGKAPWKIW

Sequences:

>Translated_252_residues
MILAGGLGTRLSEETGVRPKPMVEVGGKPILWHIMKLYSHYGIDEFIVCLGYKGYMIKEYFANYFLHMSDITFDMSRNEM
QVHQNNAEPWRVTLVDTGDVTQTGGRLRRVREYLGDDDFCMTYGDGVGDVEIDKLMAFHRAHGCLATVTATQPPGRFGAL
DLDGSSVMSFCEKPHGDGSWINGGFFVLSPKVIDYISGDSTLWEREPLERLASEKQLRAYFHHGFWQPMDTLRDKQHLES
LWQEGKAPWKIW
>Mature_252_residues
MILAGGLGTRLSEETGVRPKPMVEVGGKPILWHIMKLYSHYGIDEFIVCLGYKGYMIKEYFANYFLHMSDITFDMSRNEM
QVHQNNAEPWRVTLVDTGDVTQTGGRLRRVREYLGDDDFCMTYGDGVGDVEIDKLMAFHRAHGCLATVTATQPPGRFGAL
DLDGSSVMSFCEKPHGDGSWINGGFFVLSPKVIDYISGDSTLWEREPLERLASEKQLRAYFHHGFWQPMDTLRDKQHLES
LWQEGKAPWKIW

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=243, Percent_Identity=29.2181069958848, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI11761619, Length=243, Percent_Identity=29.2181069958848, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI133931050, Length=245, Percent_Identity=30.2040816326531, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6320148, Length=245, Percent_Identity=30.2040816326531, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21355443, Length=255, Percent_Identity=27.0588235294118, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644084, Length=255, Percent_Identity=27.0588235294118, Blast_Score=82, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 28762; Mature: 28762

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILAGGLGTRLSEETGVRPKPMVEVGGKPILWHIMKLYSHYGIDEFIVCLGYKGYMIKEY
CEEECCCCCCCCHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHH
FANYFLHMSDITFDMSRNEMQVHQNNAEPWRVTLVDTGDVTQTGGRLRRVREYLGDDDFC
HHHHHEECCCEEEECCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEE
MTYGDGVGDVEIDKLMAFHRAHGCLATVTATQPPGRFGALDLDGSSVMSFCEKPHGDGSW
EEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCHHHHHHHHHCCCCCCCE
INGGFFVLSPKVIDYISGDSTLWEREPLERLASEKQLRAYFHHGFWQPMDTLRDKQHLES
ECCCEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LWQEGKAPWKIW
HHHCCCCCCCCC
>Mature Secondary Structure
MILAGGLGTRLSEETGVRPKPMVEVGGKPILWHIMKLYSHYGIDEFIVCLGYKGYMIKEY
CEEECCCCCCCCHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHH
FANYFLHMSDITFDMSRNEMQVHQNNAEPWRVTLVDTGDVTQTGGRLRRVREYLGDDDFC
HHHHHEECCCEEEECCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEE
MTYGDGVGDVEIDKLMAFHRAHGCLATVTATQPPGRFGALDLDGSSVMSFCEKPHGDGSW
EEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCHHHHHHHHHCCCCCCCE
INGGFFVLSPKVIDYISGDSTLWEREPLERLASEKQLRAYFHHGFWQPMDTLRDKQHLES
ECCCEEEECHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LWQEGKAPWKIW
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]