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The map label for this gene is dadA [C]

Identifier: 74317884

GI number: 74317884

Start: 1961595

End: 1962701

Strand: Direct

Name: dadA [C]

Synonym: Tbd_1866

Alternate gene names: 74317884

Gene position: 1961595-1962701 (Clockwise)

Preceding gene: 74317878

Following gene: 74317885

Centisome position: 67.41

GC content: 69.29

Gene sequence:

>1107_bases
TTGACCGGCCGCACGCTCGTGGTCGGTGCCGGGGTGTCCGGCTTGGCTGTCGCACTGGCCCTGTTGCAGCGCGGGCACAA
GGTGACCCTGCTGGAGCGCGGCACGGCCGGGGCAGAGTCGTCGTGGGCGGGCGGCGGCATACTCTCGCCCCTGCTGCCGT
GGGATTATCCCGACCCCGTTTCCTCCCTCGCCTTGCGTGCGATGGGCGGCTACGCCGGCTGGGTGGACAACATCGAGGCG
ACATCCGGGCACGATGCCGAATTGTGGCGCTGTGGCATGCTCGCACTCGACGTGCCGTTCCCCGACGAGGCGCTGCGCTG
GTGTAGCGCCCACGGGATAAGGGGGGAGCCGGGCTGCCCGGAGATGCAGAACCTCGAGCGCGATGTCTGCACGGGAAATC
CGCTCTGGCTCCCCGGCATCGCCCAAGTACGTAATCCCCGGCTGGTGGCCGCGCTGCAGGCTGCGGTGACCAGAACCGGT
GGCACGATCCGCGAACACTGCCCGGCAGTCGGCGTGGTGCGTGATGGCCGACGCGTCACGGCGATACGGACTGAAGCGGA
AACACTGGCGGCTGACAGCGTCGTGCTGGCGACCGGTGCCTGGTCCGGGCTGGGACTCGAGGGCTTGGCCCCCATGCCGC
AGGTACGCCCGATCCGCGGCCAGATGCTGCTCTTCAAGCTCACCCCAGGCGCGCTCGAAACCATCCTCTATCGCAACGGG
CTCTATCTGATTCCCCGCCGTGACGGCCACGTGCTCGTCGGCAGCACGGTCGAGGACGTCGGCTTCGACAAGTCGACCGA
CGCGGCGACGCGCGCCCGACTGCACGCCGAGGCGGCGGAACTCCTGCCGGCGCTGCGGCGTGTCGAGCCGATCCGCCACT
GGGCCGGGCTGCGCCCGGGCTCGCCGGACAATATCCCGATCATCGACCGGCACCCGGATTACGACAATGTCTTCGTCAAC
ACGGGGCATTACCGCTACGGGGTGACGCTGGCGCCGGCTTCGGCCGAACTGTTGGTCGACCTGATGGAAGGTCGCACGCC
AGCGCTCGACCCCGCGCCCTATCGCTGGCACGCGGCACTCGCGCGCGAATGGCACAGAACGCTGTAG

Upstream 100 bases:

>100_bases
TAGCGCGCCTATCGAGCGCGAACATCGCCGGGGTGGACAATCCCCTCTAAAATTGCGCGGTGAACAAAAACGGCTTTTAC
GCTCCAGAAGGGAACGCTGC

Downstream 100 bases:

>100_bases
CGCCTGGCGGCAGGCCGCGAACCAGTTGGTTTACACTCGGCGCCACATGAAGTTTCTCACTGCTGCGATGTCTAATCCCC
TCCGGAACCGGTCGTGCGAC

Product: putative D-amino acid oxidase

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTGRTLVVGAGVSGLAVALALLQRGHKVTLLERGTAGAESSWAGGGILSPLLPWDYPDPVSSLALRAMGGYAGWVDNIEA
TSGHDAELWRCGMLALDVPFPDEALRWCSAHGIRGEPGCPEMQNLERDVCTGNPLWLPGIAQVRNPRLVAALQAAVTRTG
GTIREHCPAVGVVRDGRRVTAIRTEAETLAADSVVLATGAWSGLGLEGLAPMPQVRPIRGQMLLFKLTPGALETILYRNG
LYLIPRRDGHVLVGSTVEDVGFDKSTDAATRARLHAEAAELLPALRRVEPIRHWAGLRPGSPDNIPIIDRHPDYDNVFVN
TGHYRYGVTLAPASAELLVDLMEGRTPALDPAPYRWHAALAREWHRTL

Sequences:

>Translated_368_residues
MTGRTLVVGAGVSGLAVALALLQRGHKVTLLERGTAGAESSWAGGGILSPLLPWDYPDPVSSLALRAMGGYAGWVDNIEA
TSGHDAELWRCGMLALDVPFPDEALRWCSAHGIRGEPGCPEMQNLERDVCTGNPLWLPGIAQVRNPRLVAALQAAVTRTG
GTIREHCPAVGVVRDGRRVTAIRTEAETLAADSVVLATGAWSGLGLEGLAPMPQVRPIRGQMLLFKLTPGALETILYRNG
LYLIPRRDGHVLVGSTVEDVGFDKSTDAATRARLHAEAAELLPALRRVEPIRHWAGLRPGSPDNIPIIDRHPDYDNVFVN
TGHYRYGVTLAPASAELLVDLMEGRTPALDPAPYRWHAALAREWHRTL
>Mature_367_residues
TGRTLVVGAGVSGLAVALALLQRGHKVTLLERGTAGAESSWAGGGILSPLLPWDYPDPVSSLALRAMGGYAGWVDNIEAT
SGHDAELWRCGMLALDVPFPDEALRWCSAHGIRGEPGCPEMQNLERDVCTGNPLWLPGIAQVRNPRLVAALQAAVTRTGG
TIREHCPAVGVVRDGRRVTAIRTEAETLAADSVVLATGAWSGLGLEGLAPMPQVRPIRGQMLLFKLTPGALETILYRNGL
YLIPRRDGHVLVGSTVEDVGFDKSTDAATRARLHAEAAELLPALRRVEPIRHWAGLRPGSPDNIPIIDRHPDYDNVFVNT
GHYRYGVTLAPASAELLVDLMEGRTPALDPAPYRWHAALAREWHRTL

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=413, Percent_Identity=26.1501210653753, Blast_Score=102, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 39648; Mature: 39517

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGRTLVVGAGVSGLAVALALLQRGHKVTLLERGTAGAESSWAGGGILSPLLPWDYPDPV
CCCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
SSLALRAMGGYAGWVDNIEATSGHDAELWRCGMLALDVPFPDEALRWCSAHGIRGEPGCP
HHHHHHHHCCCCCHHCCCCCCCCCCHHHEEECEEEEECCCCHHHHHHHHHCCCCCCCCCC
EMQNLERDVCTGNPLWLPGIAQVRNPRLVAALQAAVTRTGGTIREHCPAVGVVRDGRRVT
HHHHCCHHHCCCCCEECCCHHHHCCCHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCEEE
AIRTEAETLAADSVVLATGAWSGLGLEGLAPMPQVRPIRGQMLLFKLTPGALETILYRNG
EEECCHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCC
LYLIPRRDGHVLVGSTVEDVGFDKSTDAATRARLHAEAAELLPALRRVEPIRHWAGLRPG
EEEEECCCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SPDNIPIIDRHPDYDNVFVNTGHYRYGVTLAPASAELLVDLMEGRTPALDPAPYRWHAAL
CCCCCCEECCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
AREWHRTL
HHHHHHCC
>Mature Secondary Structure 
TGRTLVVGAGVSGLAVALALLQRGHKVTLLERGTAGAESSWAGGGILSPLLPWDYPDPV
CCCEEEEECCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHH
SSLALRAMGGYAGWVDNIEATSGHDAELWRCGMLALDVPFPDEALRWCSAHGIRGEPGCP
HHHHHHHHCCCCCHHCCCCCCCCCCHHHEEECEEEEECCCCHHHHHHHHHCCCCCCCCCC
EMQNLERDVCTGNPLWLPGIAQVRNPRLVAALQAAVTRTGGTIREHCPAVGVVRDGRRVT
HHHHCCHHHCCCCCEECCCHHHHCCCHHHHHHHHHHHHCCCCHHHHCCEEEEEECCCEEE
AIRTEAETLAADSVVLATGAWSGLGLEGLAPMPQVRPIRGQMLLFKLTPGALETILYRNG
EEECCHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCC
LYLIPRRDGHVLVGSTVEDVGFDKSTDAATRARLHAEAAELLPALRRVEPIRHWAGLRPG
EEEEECCCCCEEECCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SPDNIPIIDRHPDYDNVFVNTGHYRYGVTLAPASAELLVDLMEGRTPALDPAPYRWHAAL
CCCCCCEECCCCCCCCEEEECCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCHHHHHH
AREWHRTL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 19 {D-serine}} 12 {D-alpha-aminobutyrate}} 6 {D-phenylalanine}} 0.15 {cytochrome} 29 {D-alanine}} 6.4 {D-alanine}} 5.3 {D

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]