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The map label for this gene is ispH [H]

Identifier: 74317878

GI number: 74317878

Start: 1954347

End: 1955273

Strand: Direct

Name: ispH [H]

Synonym: Tbd_1860

Alternate gene names: 74317878

Gene position: 1954347-1955273 (Clockwise)

Preceding gene: 74317877

Following gene: 74317884

Centisome position: 67.16

GC content: 64.29

Gene sequence:

>927_bases
ATGACCCCCACCGTTCTGCTCGCCAATCCGCGCGGCTTCTGCGCCGGCGTCGACCGCGCGATCGCGATCGTCGAACGCGC
GCTGGAAAAATTCGGCGCACCGATCTACGTGCGCCACGAAGTCGTCCACAACACCTTCGTCGTCGACGACCTCAAGCGCA
AGGGTGCCGTTTTTGTCGAGGAACTGTCCGAGGTGCCGGCCGGCGCGACCGTCATTTTCAGTGCACACGGCGTGTCTCAA
GCTGTGCGCACTGAAGCCGCAGCCCGCGGGCTGACCGTGTTCGACGCGACCTGCCCGCTTGTCACCAAGGTCCACGTCGA
AGTCAGCAAGATGCGCGACAAGGGATACGAGATCGTGATGATCGGCCACAAAGGACACCCCGAGGTCGAAGGCACGCTCG
GCCAGTCCGCCGGCGGCATGTACCTCGTCGAGACGCCCGGCGACGTCGCGACGCTCGAGGTAACGAATCCCGACTGCCTC
GCCTACGTTACCCAGACTACACTCTCGGTCGACGACGCCGCACGCGTGGTCGATGCCCTGCGCGCGCGCTTCCCCACGAT
CACGGGGCCAAAGAAGGACGACATCTGTTATGCGACGCAAAACCGCCAGGATGCAGTGAAGCATCTGGCGTCGCAGTGCG
ACGTCGTCATCGTCGTGGGATCGCCGACCAGTTCGAATTCGAACCGCCTGCGGGAGGTCGCGGAGAACCTGGGGATAGCG
GCCTACATGGTCGACAATGCCGACGAGATCGACCCGCGGTGGGTATCGGGAAACCAACGCGTCGGCGTGACCGCCGGGGC
GTCGGCACCCGACGTGCTGGTGCGGGCAGTGATCGATCGCCTGGAACGACTGGGGATATGTTCGGTGAGCGAGCTCGATG
GGGTGCAGGAGAAGGTCGGATTCCCTCTGCCGAGAGAGTTGGCGTAA

Upstream 100 bases:

>100_bases
GCCGACGACGCGATCAAGGTCGACTTCAATCACCCTCTCGCCGACCTCTCGATCGAATTCGAGGTCGAAGTCGTTCGCAT
CCTCTGACGGAAGATCGTTC

Downstream 100 bases:

>100_bases
AAATAGCCTTGGGCAGGGCGGTGCCAAGCCGGCACCTGCGTGGCCCCGACAAATGGTCACCTCAGGGCAATTTTCCTGCG
GTCGCGCCGGGAAACCTGCC

Product: 4-hydroxy-3-methylbut-2-enyl diphosphate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MTPTVLLANPRGFCAGVDRAIAIVERALEKFGAPIYVRHEVVHNTFVVDDLKRKGAVFVEELSEVPAGATVIFSAHGVSQ
AVRTEAAARGLTVFDATCPLVTKVHVEVSKMRDKGYEIVMIGHKGHPEVEGTLGQSAGGMYLVETPGDVATLEVTNPDCL
AYVTQTTLSVDDAARVVDALRARFPTITGPKKDDICYATQNRQDAVKHLASQCDVVIVVGSPTSSNSNRLREVAENLGIA
AYMVDNADEIDPRWVSGNQRVGVTAGASAPDVLVRAVIDRLERLGICSVSELDGVQEKVGFPLPRELA

Sequences:

>Translated_308_residues
MTPTVLLANPRGFCAGVDRAIAIVERALEKFGAPIYVRHEVVHNTFVVDDLKRKGAVFVEELSEVPAGATVIFSAHGVSQ
AVRTEAAARGLTVFDATCPLVTKVHVEVSKMRDKGYEIVMIGHKGHPEVEGTLGQSAGGMYLVETPGDVATLEVTNPDCL
AYVTQTTLSVDDAARVVDALRARFPTITGPKKDDICYATQNRQDAVKHLASQCDVVIVVGSPTSSNSNRLREVAENLGIA
AYMVDNADEIDPRWVSGNQRVGVTAGASAPDVLVRAVIDRLERLGICSVSELDGVQEKVGFPLPRELA
>Mature_307_residues
TPTVLLANPRGFCAGVDRAIAIVERALEKFGAPIYVRHEVVHNTFVVDDLKRKGAVFVEELSEVPAGATVIFSAHGVSQA
VRTEAAARGLTVFDATCPLVTKVHVEVSKMRDKGYEIVMIGHKGHPEVEGTLGQSAGGMYLVETPGDVATLEVTNPDCLA
YVTQTTLSVDDAARVVDALRARFPTITGPKKDDICYATQNRQDAVKHLASQCDVVIVVGSPTSSNSNRLREVAENLGIAA
YMVDNADEIDPRWVSGNQRVGVTAGASAPDVLVRAVIDRLERLGICSVSELDGVQEKVGFPLPRELA

Specific function: Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) [H]

COG id: COG0761

COG function: function code IM; Penicillin tolerance protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispH family [H]

Homologues:

Organism=Escherichia coli, GI1786212, Length=306, Percent_Identity=62.7450980392157, Blast_Score=398, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003451 [H]

Pfam domain/function: PF02401 LYTB [H]

EC number: =1.17.1.2 [H]

Molecular weight: Translated: 32959; Mature: 32828

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPTVLLANPRGFCAGVDRAIAIVERALEKFGAPIYVRHEVVHNTFVVDDLKRKGAVFVE
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCHHHH
ELSEVPAGATVIFSAHGVSQAVRTEAAARGLTVFDATCPLVTKVHVEVSKMRDKGYEIVM
HHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEEEHHHHHHHCCCCEEEE
IGHKGHPEVEGTLGQSAGGMYLVETPGDVATLEVTNPDCLAYVTQTTLSVDDAARVVDAL
ECCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEEEHHHHCCHHHHHHHHHHH
RARFPTITGPKKDDICYATQNRQDAVKHLASQCDVVIVVGSPTSSNSNRLREVAENLGIA
HHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCEE
AYMVDNADEIDPRWVSGNQRVGVTAGASAPDVLVRAVIDRLERLGICSVSELDGVQEKVG
EEEECCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHC
FPLPRELA
CCCCCCCC
>Mature Secondary Structure 
TPTVLLANPRGFCAGVDRAIAIVERALEKFGAPIYVRHEVVHNTFVVDDLKRKGAVFVE
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCCCHHHH
ELSEVPAGATVIFSAHGVSQAVRTEAAARGLTVFDATCPLVTKVHVEVSKMRDKGYEIVM
HHHHCCCCEEEEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEEEHHHHHHHCCCCEEEE
IGHKGHPEVEGTLGQSAGGMYLVETPGDVATLEVTNPDCLAYVTQTTLSVDDAARVVDAL
ECCCCCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCEEEEHHHHCCHHHHHHHHHHH
RARFPTITGPKKDDICYATQNRQDAVKHLASQCDVVIVVGSPTSSNSNRLREVAENLGIA
HHHCCCCCCCCCCCEEEECCCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHCCEE
AYMVDNADEIDPRWVSGNQRVGVTAGASAPDVLVRAVIDRLERLGICSVSELDGVQEKVG
EEEECCCCCCCCEEECCCCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHCCHHHHHC
FPLPRELA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA