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The map label for this gene is hsdFM [H]

Identifier: 74317828

GI number: 74317828

Start: 1912766

End: 1913914

Strand: Direct

Name: hsdFM [H]

Synonym: Tbd_1810

Alternate gene names: 74317828

Gene position: 1912766-1913914 (Clockwise)

Preceding gene: 74317827

Following gene: 74317829

Centisome position: 65.74

GC content: 65.54

Gene sequence:

>1149_bases
ATGCCTAGCTTTTACGAATTCTTCGCCGGCGGCGGCATGGCCCGCGCCGGCTTGGGTGCCGAATGGGATTGCCTATTCGC
CAACGATTTCGATCCGAAGAAGGCGGCGAGCTACGCCGTCAATTGGGACGGCGATGAGTTACGCGTCGGCGACGTGGGGA
CGCTCACAGTCCCCGATCTGCCAGGCCGCGCTGATCTCGCTTGGGCGTCATTTCCATGCCAGGACCTTTCCCTAGCCGGC
GCGGGCGCCGGCCTCAAGGGGGAGCGCTCGGGCACGTTCTGGCCGTTCTGGAAGCTGGTGAAGGCGCTAGGCGATGAAGA
CCGCGCCCCACGCCTGGTCGTCCTCGAAAACGTCTACGGTGCCCTCAGTTCGCACGGCGGAAAGGACTTTGCGGCGATCT
GCGCCGCGCTCGCGGGCGCGGGCTACCGTTTCGGCGCGGTGATCGTGAACGCGGTCCACTTCCTCCCGCAGTCGCGTCCC
CGACTGTTTATCATCGGCGCGCTCAAGTCGATGCCCGTGCCTCTGGGACTCGTCGCCGGCGGCCCAGCCGGCGAATGGCA
TCCGGCGGCGCTGGTAGAAGCCTATGGCAAGCTCTCCAGACGATCACAAGGCGCGTGGGTGTGGTGGGACCTTCCCGCGC
CGCCGATCCGCGCCTCGATCTTCGCCGATTTAGTGGAAGACGATCCCCAGGGCGTCACTTGGCACACGCCGGCGGAAACC
AAGAAGCTACTTGCCTTGATGAATCCCCATAACCTGGCGAAGGTCGAAGCTGCGAAGAAAGCCGGCCGCCGCATGGTCGG
CACGATCTACAAGCGCACGCGCGCCGACGGTCCCAATGGCGAGAAAGCTCAGCGGGCAGAAATTCGCTTTGATGATATCG
CCGGCTGCCTGCGCACGCCCGTAGGCGGCTCCAGTCGGCAAACCATCATGGTCATCGAAGGCAAGCGCGTCCGCTCGCGC
TTGCTGTCGCCGCGCGAAGCCGCTCGCCTCATGGGCCTGCCGGATACCTACATGTTGCCGAAGAACTACAACGAGGCCTA
TCACCTCGCCGGCGACGGCGTGGCCGTGCCGGTGGTGCGCTTCTTGGCCGACAGCGTCCTAGAGCCGCTGCTGGCCGCCG
CCCTTGCGACCGAGAAGAAGGCGGCGTAG

Upstream 100 bases:

>100_bases
TTGCGAGAGTTCATGCGAACTTTTGCGGACCAACATCAAGATGGCAGGCAAGCAAGCCTGTTTGCAACCCCGCCCTTGAA
ACAAGGCAGGAGAAGGAACA

Downstream 100 bases:

>100_bases
TGGCGGACTTGCGAAGCGCGCTCCAGACCTTTAATAAGGAATGGAAATTCAGCAGCAAAGGTCCGCTGTGCGTGGCGCTG
GTCATCACTCAGCACGCACG

Product: C-5 cytosine-specific DNA methylase

Products: NA

Alternate protein names: M.BsuFI; Cytosine-specific methyltransferase BsuFI [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MPSFYEFFAGGGMARAGLGAEWDCLFANDFDPKKAASYAVNWDGDELRVGDVGTLTVPDLPGRADLAWASFPCQDLSLAG
AGAGLKGERSGTFWPFWKLVKALGDEDRAPRLVVLENVYGALSSHGGKDFAAICAALAGAGYRFGAVIVNAVHFLPQSRP
RLFIIGALKSMPVPLGLVAGGPAGEWHPAALVEAYGKLSRRSQGAWVWWDLPAPPIRASIFADLVEDDPQGVTWHTPAET
KKLLALMNPHNLAKVEAAKKAGRRMVGTIYKRTRADGPNGEKAQRAEIRFDDIAGCLRTPVGGSSRQTIMVIEGKRVRSR
LLSPREAARLMGLPDTYMLPKNYNEAYHLAGDGVAVPVVRFLADSVLEPLLAAALATEKKAA

Sequences:

>Translated_382_residues
MPSFYEFFAGGGMARAGLGAEWDCLFANDFDPKKAASYAVNWDGDELRVGDVGTLTVPDLPGRADLAWASFPCQDLSLAG
AGAGLKGERSGTFWPFWKLVKALGDEDRAPRLVVLENVYGALSSHGGKDFAAICAALAGAGYRFGAVIVNAVHFLPQSRP
RLFIIGALKSMPVPLGLVAGGPAGEWHPAALVEAYGKLSRRSQGAWVWWDLPAPPIRASIFADLVEDDPQGVTWHTPAET
KKLLALMNPHNLAKVEAAKKAGRRMVGTIYKRTRADGPNGEKAQRAEIRFDDIAGCLRTPVGGSSRQTIMVIEGKRVRSR
LLSPREAARLMGLPDTYMLPKNYNEAYHLAGDGVAVPVVRFLADSVLEPLLAAALATEKKAA
>Mature_381_residues
PSFYEFFAGGGMARAGLGAEWDCLFANDFDPKKAASYAVNWDGDELRVGDVGTLTVPDLPGRADLAWASFPCQDLSLAGA
GAGLKGERSGTFWPFWKLVKALGDEDRAPRLVVLENVYGALSSHGGKDFAAICAALAGAGYRFGAVIVNAVHFLPQSRPR
LFIIGALKSMPVPLGLVAGGPAGEWHPAALVEAYGKLSRRSQGAWVWWDLPAPPIRASIFADLVEDDPQGVTWHTPAETK
KLLALMNPHNLAKVEAAKKAGRRMVGTIYKRTRADGPNGEKAQRAEIRFDDIAGCLRTPVGGSSRQTIMVIEGKRVRSRL
LSPREAARLMGLPDTYMLPKNYNEAYHLAGDGVAVPVVRFLADSVLEPLLAAALATEKKAA

Specific function: This methylase recognizes the double-stranded sequence CCGG, causes specific methylation on C-1 on both strands, and protects the DNA from cleavage by the BsuFI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 40989; Mature: 40858

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSFYEFFAGGGMARAGLGAEWDCLFANDFDPKKAASYAVNWDGDELRVGDVGTLTVPDL
CCCHHHHHCCCCCEECCCCCCEEEEECCCCCCHHHHCEEEECCCCEEEECCCCEEECCCC
PGRADLAWASFPCQDLSLAGAGAGLKGERSGTFWPFWKLVKALGDEDRAPRLVVLENVYG
CCCCCEEECCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHH
ALSSHGGKDFAAICAALAGAGYRFGAVIVNAVHFLPQSRPRLFIIGALKSMPVPLGLVAG
HHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHCCCCCEEEEEC
GPAGEWHPAALVEAYGKLSRRSQGAWVWWDLPAPPIRASIFADLVEDDPQGVTWHTPAET
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEECCCHHH
KKLLALMNPHNLAKVEAAKKAGRRMVGTIYKRTRADGPNGEKAQRAEIRFDDIAGCLRTP
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHCCC
VGGSSRQTIMVIEGKRVRSRLLSPREAARLMGLPDTYMLPKNYNEAYHLAGDGVAVPVVR
CCCCCCCEEEEEECHHHHHHCCCHHHHHHHCCCCCCEECCCCCCCEEEECCCCHHHHHHH
FLADSVLEPLLAAALATEKKAA
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSFYEFFAGGGMARAGLGAEWDCLFANDFDPKKAASYAVNWDGDELRVGDVGTLTVPDL
CCHHHHHCCCCCEECCCCCCEEEEECCCCCCHHHHCEEEECCCCEEEECCCCEEECCCC
PGRADLAWASFPCQDLSLAGAGAGLKGERSGTFWPFWKLVKALGDEDRAPRLVVLENVYG
CCCCCEEECCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEHHHHH
ALSSHGGKDFAAICAALAGAGYRFGAVIVNAVHFLPQSRPRLFIIGALKSMPVPLGLVAG
HHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEEEECHHCCCCCEEEEEC
GPAGEWHPAALVEAYGKLSRRSQGAWVWWDLPAPPIRASIFADLVEDDPQGVTWHTPAET
CCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHCCCCCCCEECCCHHH
KKLLALMNPHNLAKVEAAKKAGRRMVGTIYKRTRADGPNGEKAQRAEIRFDDIAGCLRTP
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHCCC
VGGSSRQTIMVIEGKRVRSRLLSPREAARLMGLPDTYMLPKNYNEAYHLAGDGVAVPVVR
CCCCCCCEEEEEECHHHHHHCCCHHHHHHHCCCCCCEECCCCCCCEEEECCCCHHHHHHH
FLADSVLEPLLAAALATEKKAA
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2108858 [H]