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The map label for this gene is trpB2 [H]

Identifier: 74317791

GI number: 74317791

Start: 1872187

End: 1873539

Strand: Reverse

Name: trpB2 [H]

Synonym: Tbd_1773

Alternate gene names: 74317791

Gene position: 1873539-1872187 (Counterclockwise)

Preceding gene: 74317792

Following gene: 74317788

Centisome position: 64.39

GC content: 65.19

Gene sequence:

>1353_bases
ATGCCGCAGACCAAATTTCTACTCGACGAGTCCGAGATTCCGAGCCACTGGTACAACGTCGTCGCCGACATGCCCAAGCC
GCCGGCGCCCCCGCTGGGGCCCGACGGAAAACCGGTGGGCCCCGACGCCCTGAGCGCGATTTTCCCGCAATCGCTGATCG
AGCAGGAGATGTCGGCGGAACGCTGGATCGCGATCCCCGAGAAGGTGCGCGAGATCTATCGACTGTGGCGCCCGTCACCT
TTGTTCCGCGCGCACCGGCTCGAAGCCGCGCTCGGCACGCCGGCGAAGATCTACTACAAGTACGAAGGCGTTTCGCCGGC
CGGCTCGCACAAGGTCAACACCTCGGTCGCGCAGGCCTATTACAACGCTGAGGCTGGGATCAAGCGCATCGCGACCGAGA
CCGGCGCCGGCCAATGGGGTTGTTCGATGGCGCTCGCGGGCCAGATGTTCGGGCTCGAGGTGCGCGTCTACATGGTGAAG
GTCAGTTACAACCAGAAACCCTACCGGCGCTCGATGATGCAGACCTGGGGCGCCGAAGTGTTTGCGAGCCCGAGCACGGA
AACCGCATCGGGGCGCGCGGCGCTGGCCGAAAGCGCCGACAACCCCGGCTCGCTCGGCCTCGCCATCTCCGAGGCGGTCG
AGGACGCAGCGTCGCGCGCCGACACGAGCTATGCGCTCGGGTCGGTCCTCAATCACGTCTTGCTGCACCAGACCGTGATC
GGGCTCGAGGCGAAGAAGCAATTCGAGAAGGCGGGTGATTATCCCGACATGATCTTCGCGCCCTGCGGCGGCGGGTCCAA
CTTCGGCGGCGTCGCCTTTCCTTTTCTCGCCGACAAGGCCGCGGGCAAAAACGTGCGCCTGGTCGCGGTCGAACCGGCGT
CCTGCCCGACGCTCACGCGCGGCCACTATGCCTACGACTTCGGCGACACGGCGAAGCTGACGCCGATGATGCTGATGTAC
ACCCTCGGCCACGACTTCATGCCCCCGGGTATCCACGCGGGTGGCCTGCGCTATCACGGCGACTCCGCGCTCGTTTCGCA
GCTTTACCACGAAAAGCTGCTCGAGGCGGTCGCCGTACAGCAGCTTGCGACCTTCGAGGCCGGCGTACGCTTTGCACGCG
CCGAAGGCATCGTCCCAGCGCCCGAATCCAATCACGCGATCGCGGCATGCATCGACGAAGCCCTGCGTTGTAAGGAAAGC
GGCGAAGCGAAGACGCTGTTTTTCAACCTCTCGGGCCACGGCCATTTCGACATGGCCGCCTACGACAGCTATTTCGCGGG
CAAGCTCGAGGATTTCGCGTATCCCGAAGCGGCGATCCAGGCCGCGCTCGAGCGCCTGCCCAAGGTCGCGTAA

Upstream 100 bases:

>100_bases
ACGCCCGCCCCGGCGCGCCGATGCGGGCCAGGCGAGATCAGGCGCGCTATGATCTGCTGACCGGGGGCGATCCCGGCGCA
GACAATCACGGAGATACCTC

Downstream 100 bases:

>100_bases
GCCCTGTCATTCCGCCGACAAGAGGGCGGCCTCGTGCCGCCCTCAGCGTTGGCCGAATACGCCGCGCCCGCGGAAATACT
GCACCCCCAGCCAGCCGCCG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MPQTKFLLDESEIPSHWYNVVADMPKPPAPPLGPDGKPVGPDALSAIFPQSLIEQEMSAERWIAIPEKVREIYRLWRPSP
LFRAHRLEAALGTPAKIYYKYEGVSPAGSHKVNTSVAQAYYNAEAGIKRIATETGAGQWGCSMALAGQMFGLEVRVYMVK
VSYNQKPYRRSMMQTWGAEVFASPSTETASGRAALAESADNPGSLGLAISEAVEDAASRADTSYALGSVLNHVLLHQTVI
GLEAKKQFEKAGDYPDMIFAPCGGGSNFGGVAFPFLADKAAGKNVRLVAVEPASCPTLTRGHYAYDFGDTAKLTPMMLMY
TLGHDFMPPGIHAGGLRYHGDSALVSQLYHEKLLEAVAVQQLATFEAGVRFARAEGIVPAPESNHAIAACIDEALRCKES
GEAKTLFFNLSGHGHFDMAAYDSYFAGKLEDFAYPEAAIQAALERLPKVA

Sequences:

>Translated_450_residues
MPQTKFLLDESEIPSHWYNVVADMPKPPAPPLGPDGKPVGPDALSAIFPQSLIEQEMSAERWIAIPEKVREIYRLWRPSP
LFRAHRLEAALGTPAKIYYKYEGVSPAGSHKVNTSVAQAYYNAEAGIKRIATETGAGQWGCSMALAGQMFGLEVRVYMVK
VSYNQKPYRRSMMQTWGAEVFASPSTETASGRAALAESADNPGSLGLAISEAVEDAASRADTSYALGSVLNHVLLHQTVI
GLEAKKQFEKAGDYPDMIFAPCGGGSNFGGVAFPFLADKAAGKNVRLVAVEPASCPTLTRGHYAYDFGDTAKLTPMMLMY
TLGHDFMPPGIHAGGLRYHGDSALVSQLYHEKLLEAVAVQQLATFEAGVRFARAEGIVPAPESNHAIAACIDEALRCKES
GEAKTLFFNLSGHGHFDMAAYDSYFAGKLEDFAYPEAAIQAALERLPKVA
>Mature_449_residues
PQTKFLLDESEIPSHWYNVVADMPKPPAPPLGPDGKPVGPDALSAIFPQSLIEQEMSAERWIAIPEKVREIYRLWRPSPL
FRAHRLEAALGTPAKIYYKYEGVSPAGSHKVNTSVAQAYYNAEAGIKRIATETGAGQWGCSMALAGQMFGLEVRVYMVKV
SYNQKPYRRSMMQTWGAEVFASPSTETASGRAALAESADNPGSLGLAISEAVEDAASRADTSYALGSVLNHVLLHQTVIG
LEAKKQFEKAGDYPDMIFAPCGGGSNFGGVAFPFLADKAAGKNVRLVAVEPASCPTLTRGHYAYDFGDTAKLTPMMLMYT
LGHDFMPPGIHAGGLRYHGDSALVSQLYHEKLLEAVAVQQLATFEAGVRFARAEGIVPAPESNHAIAACIDEALRCKESG
EAKTLFFNLSGHGHFDMAAYDSYFAGKLEDFAYPEAAIQAALERLPKVA

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine [H]

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family [H]

Homologues:

Organism=Escherichia coli, GI1787515, Length=368, Percent_Identity=31.25, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321412, Length=400, Percent_Identity=30.25, Blast_Score=106, Evalue=6e-24,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 48615; Mature: 48483

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQTKFLLDESEIPSHWYNVVADMPKPPAPPLGPDGKPVGPDALSAIFPQSLIEQEMSAE
CCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHH
RWIAIPEKVREIYRLWRPSPLFRAHRLEAALGTPAKIYYKYEGVSPAGSHKVNTSVAQAY
HEEECHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHH
YNAEAGIKRIATETGAGQWGCSMALAGQMFGLEVRVYMVKVSYNQKPYRRSMMQTWGAEV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEEEEEEEECCCCHHHHHHHHHCCCCE
FASPSTETASGRAALAESADNPGSLGLAISEAVEDAASRADTSYALGSVLNHVLLHQTVI
EECCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GLEAKKQFEKAGDYPDMIFAPCGGGSNFGGVAFPFLADKAAGKNVRLVAVEPASCPTLTR
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEHHHHHHHCCCCCEEEEEECCCCCCCCCC
GHYAYDFGDTAKLTPMMLMYTLGHDFMPPGIHAGGLRYHGDSALVSQLYHEKLLEAVAVQ
CCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHH
QLATFEAGVRFARAEGIVPAPESNHAIAACIDEALRCKESGEAKTLFFNLSGHGHFDMAA
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHH
YDSYFAGKLEDFAYPEAAIQAALERLPKVA
HHHHHHCCHHHHCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PQTKFLLDESEIPSHWYNVVADMPKPPAPPLGPDGKPVGPDALSAIFPQSLIEQEMSAE
CCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHH
RWIAIPEKVREIYRLWRPSPLFRAHRLEAALGTPAKIYYKYEGVSPAGSHKVNTSVAQAY
HEEECHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCHHHHHHHH
YNAEAGIKRIATETGAGQWGCSMALAGQMFGLEVRVYMVKVSYNQKPYRRSMMQTWGAEV
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEEEEEEEECCCCHHHHHHHHHCCCCE
FASPSTETASGRAALAESADNPGSLGLAISEAVEDAASRADTSYALGSVLNHVLLHQTVI
EECCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
GLEAKKQFEKAGDYPDMIFAPCGGGSNFGGVAFPFLADKAAGKNVRLVAVEPASCPTLTR
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEHHHHHHHCCCCCEEEEEECCCCCCCCCC
GHYAYDFGDTAKLTPMMLMYTLGHDFMPPGIHAGGLRYHGDSALVSQLYHEKLLEAVAVQ
CCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHH
QLATFEAGVRFARAEGIVPAPESNHAIAACIDEALRCKESGEAKTLFFNLSGHGHFDMAA
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCHHH
YDSYFAGKLEDFAYPEAAIQAALERLPKVA
HHHHHHCCHHHHCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]