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The map label for this gene is adh1 [H]

Identifier: 74317785

GI number: 74317785

Start: 1866050

End: 1867237

Strand: Direct

Name: adh1 [H]

Synonym: Tbd_1767

Alternate gene names: 74317785

Gene position: 1866050-1867237 (Clockwise)

Preceding gene: 74317781

Following gene: 74317786

Centisome position: 64.13

GC content: 67.68

Gene sequence:

>1188_bases
ATGCACGTCACTTCTTTCTCGCTCTCCCGTCTGCCGCGTATCGAGTTCGGGGCGGGCAGTATCGGTCGACTTCCAGCGAT
GGTCCGGCAGTATGGGACGCGCGCCCTCCTCGTGACGGGAGGCGCCTCGTTCGAGCGGACGGCCGCCTGGCCGGCGCTGC
TGGCCGGCCTGCGCGCACACGGCGTCGAATGGTCGCGCATCCGTGTGGCTGGGGAGCCTTCGCCGGATCTCATCGACCGC
GCGGTGCGCGACCTCCAGTCCGAAAACGTGGAGGTGGTGATCGGGATCGGGGGCGGCAGCGCGCTCGATGCAGCCAAGGC
AATTGCCGGGCTCCTCCGGACCGGGAACTCGGTCATGGATCATCTGGAGGGCGTCGGTCCGGAGTTGCCCTACACCGGCC
CGGCCGTTCCCTTCATCGCGGTCCCGACCACCGCGGGTACCGGCTCCGAGGCGACCAAGAATGCGGTCTTGAGCGTGCAA
GGGACGCACGGCTTCAAGAAGTCCTTCCGCGACGAGAAACTTGTCGCCGAATTCGCCGTGGTCGACCCTGACCTCCTCGC
GACCTGTCCACCTGCGGTCGTCGCGGCAAACGGCATGGACGCGTTCACGCAGTTGCTCGAGTCTTTCATTTCGAGCCGGG
CCGCGCCTTTTACCGACTCGATCGCCTGGGGCGGGATGAAGGCTGCGCGCGACGGCTTGCTCGCGCTGCACGCCGACCAG
GGCGACGCGGCCGCTCGCGAGCGCATGGCTTACGCCGCGCTCGCATCGGGGATCACGCTCGCGCAGGCCGGCCTCGGCTC
GGTGCACGGGCTGGCTGCTCCGCTCGGCGCCTTCTTCCCGATTCCGCACGGCCTCGCCTGCGGCACGCTCCTCGCGGAGG
CGACGGAGATGAACCTCGGCGCGCTACGTGCCCGCGCGCCTTCGCACCCGGCCCTCGGGAAGTACGCCCAGGTCGGTCGT
CTACTCGGCAAAGACGGCCGACTCGCAGATGCTGATGCCCAAAATCGCCTGGTTTCGATACTCCGCGCGTGGACCGAAAC
GCTCGGCTTGCCCACCCTGGGCCACTACGGCGTGACCGCCTCCGACATTCCTCGCATCGTCGCGAATAGTCGCGGAAGCA
GTATGAGGACCAATCCGGTCGTTCTCGACGACGGAGAGATTGCAGGAATCCTCGAAGCTCGGATCTGA

Upstream 100 bases:

>100_bases
CGACGCCGCTCCCGGGCTTTACCAGAGCACTTTTCCGAAAAAAACATCAGCCATTGAAACGAACTACCATGGACCTCTTC
ATTAAACCGTGAGTCTGCGA

Downstream 100 bases:

>100_bases
GCCGTCGCGCGCCTGATCGCAGCGTCGCCGACCCGCGCCGAGTACGCGGTTTCCGAACTCAGGACCCACGAGGACGAGCG
AGAAGCGTCGCGCATTGAGG

Product: iron-containing alcohol dehydrogenase

Products: aldehyde; ketone; NADH; H+

Alternate protein names: BDH [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MHVTSFSLSRLPRIEFGAGSIGRLPAMVRQYGTRALLVTGGASFERTAAWPALLAGLRAHGVEWSRIRVAGEPSPDLIDR
AVRDLQSENVEVVIGIGGGSALDAAKAIAGLLRTGNSVMDHLEGVGPELPYTGPAVPFIAVPTTAGTGSEATKNAVLSVQ
GTHGFKKSFRDEKLVAEFAVVDPDLLATCPPAVVAANGMDAFTQLLESFISSRAAPFTDSIAWGGMKAARDGLLALHADQ
GDAAARERMAYAALASGITLAQAGLGSVHGLAAPLGAFFPIPHGLACGTLLAEATEMNLGALRARAPSHPALGKYAQVGR
LLGKDGRLADADAQNRLVSILRAWTETLGLPTLGHYGVTASDIPRIVANSRGSSMRTNPVVLDDGEIAGILEARI

Sequences:

>Translated_395_residues
MHVTSFSLSRLPRIEFGAGSIGRLPAMVRQYGTRALLVTGGASFERTAAWPALLAGLRAHGVEWSRIRVAGEPSPDLIDR
AVRDLQSENVEVVIGIGGGSALDAAKAIAGLLRTGNSVMDHLEGVGPELPYTGPAVPFIAVPTTAGTGSEATKNAVLSVQ
GTHGFKKSFRDEKLVAEFAVVDPDLLATCPPAVVAANGMDAFTQLLESFISSRAAPFTDSIAWGGMKAARDGLLALHADQ
GDAAARERMAYAALASGITLAQAGLGSVHGLAAPLGAFFPIPHGLACGTLLAEATEMNLGALRARAPSHPALGKYAQVGR
LLGKDGRLADADAQNRLVSILRAWTETLGLPTLGHYGVTASDIPRIVANSRGSSMRTNPVVLDDGEIAGILEARI
>Mature_395_residues
MHVTSFSLSRLPRIEFGAGSIGRLPAMVRQYGTRALLVTGGASFERTAAWPALLAGLRAHGVEWSRIRVAGEPSPDLIDR
AVRDLQSENVEVVIGIGGGSALDAAKAIAGLLRTGNSVMDHLEGVGPELPYTGPAVPFIAVPTTAGTGSEATKNAVLSVQ
GTHGFKKSFRDEKLVAEFAVVDPDLLATCPPAVVAANGMDAFTQLLESFISSRAAPFTDSIAWGGMKAARDGLLALHADQ
GDAAARERMAYAALASGITLAQAGLGSVHGLAAPLGAFFPIPHGLACGTLLAEATEMNLGALRARAPSHPALGKYAQVGR
LLGKDGRLADADAQNRLVSILRAWTETLGLPTLGHYGVTASDIPRIVANSRGSSMRTNPVVLDDGEIAGILEARI

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082107, Length=340, Percent_Identity=31.7647058823529, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789163, Length=354, Percent_Identity=30.5084745762712, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI48994951, Length=389, Percent_Identity=29.8200514138817, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787493, Length=379, Percent_Identity=30.8707124010554, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789386, Length=395, Percent_Identity=28.8607594936709, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17537053, Length=390, Percent_Identity=26.4102564102564, Blast_Score=99, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321181, Length=325, Percent_Identity=30.7692307692308, Blast_Score=153, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24657991, Length=395, Percent_Identity=26.3291139240506, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1

Molecular weight: Translated: 40915; Mature: 40915

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHVTSFSLSRLPRIEFGAGSIGRLPAMVRQYGTRALLVTGGASFERTAAWPALLAGLRAH
CCCCCCHHHHCCCEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHC
GVEWSRIRVAGEPSPDLIDRAVRDLQSENVEVVIGIGGGSALDAAKAIAGLLRTGNSVMD
CCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHH
HLEGVGPELPYTGPAVPFIAVPTTAGTGSEATKNAVLSVQGTHGFKKSFRDEKLVAEFAV
HHHCCCCCCCCCCCCCCEEEECCCCCCCCCHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
VDPDLLATCPPAVVAANGMDAFTQLLESFISSRAAPFTDSIAWGGMKAARDGLLALHADQ
CCCHHHHCCCCHHEECCCHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHCCCEEEEECCC
GDAAARERMAYAALASGITLAQAGLGSVHGLAAPLGAFFPIPHGLACGTLLAEATEMNLG
CCHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHH
ALRARAPSHPALGKYAQVGRLLGKDGRLADADAQNRLVSILRAWTETLGLPTLGHYGVTA
HHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
SDIPRIVANSRGSSMRTNPVVLDDGEIAGILEARI
HHHHHHHHCCCCCCCCCCCEEEECCCEECCEECCC
>Mature Secondary Structure
MHVTSFSLSRLPRIEFGAGSIGRLPAMVRQYGTRALLVTGGASFERTAAWPALLAGLRAH
CCCCCCHHHHCCCEEECCCCHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHC
GVEWSRIRVAGEPSPDLIDRAVRDLQSENVEVVIGIGGGSALDAAKAIAGLLRTGNSVMD
CCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHH
HLEGVGPELPYTGPAVPFIAVPTTAGTGSEATKNAVLSVQGTHGFKKSFRDEKLVAEFAV
HHHCCCCCCCCCCCCCCEEEECCCCCCCCCHHCCCEEEEECCCHHHHHHHHHHHHHHHHH
VDPDLLATCPPAVVAANGMDAFTQLLESFISSRAAPFTDSIAWGGMKAARDGLLALHADQ
CCCHHHHCCCCHHEECCCHHHHHHHHHHHHHHCCCCCCCCHHHCCHHHHCCCEEEEECCC
GDAAARERMAYAALASGITLAQAGLGSVHGLAAPLGAFFPIPHGLACGTLLAEATEMNLG
CCHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHH
ALRARAPSHPALGKYAQVGRLLGKDGRLADADAQNRLVSILRAWTETLGLPTLGHYGVTA
HHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
SDIPRIVANSRGSSMRTNPVVLDDGEIAGILEARI
HHHHHHHHCCCCCCCCCCCEEEECCCEECCEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+; NADH [C]

Metal ions: Co2+ Zn2+-depleted [C]

Kcat value (1/min): 150 [C]

Specific activity: 19

Km value (mM): 0.2 {tryptophol}} 0.0074 {NAD+}} 0.0069 {NAD+}} 13 {ethylene} 6 {methanol}} 27 {pentanol}} 0.44 {1-butanol}} 120 {ethanol}} 0.91 {acetaldehyde}} 0.1 {acetaldehyde}} 0.005 {1-octanol}} 0.004 {benzyl} 0.005 {cyclohe

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: 2, 2'-Bipyridyl; 4-Methyl pyrazole acetaldehyde; 8-Amino-6-methoxyquinoline; 8-Hydroxy quinoline-5-sulfonic acid; Acetaldehyde; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]