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The map label for this gene is hmeA [H]

Identifier: 74317757

GI number: 74317757

Start: 1825436

End: 1826173

Strand: Direct

Name: hmeA [H]

Synonym: Tbd_1739

Alternate gene names: 74317757

Gene position: 1825436-1826173 (Clockwise)

Preceding gene: 74317752

Following gene: 74317758

Centisome position: 62.73

GC content: 68.16

Gene sequence:

>738_bases
ATGTCCGACAACCCTTCCCCCTCCCGCCGCGCCTTCCTGCAGGCGATCCCGGCGATCACCGCGGGTCTCGCGCTGCCGGC
GACCGCACGCGCGGCGCCCCACAAACACCACTGGGCGATGCTGGTCGACGTCCGCAAGTGTATCGGCTGTCAGGCCTGCA
CCGTCGCGTGCATCATGGAAAACGCGCTACCGGAAAACAGTTTCCGCACGCTCGCCTCCACCTACGTCGTGCAGCAGGAG
ACCGAGGGCCGGACCACGGCCGGCACCTACATGCTGCCGCGCCTGTGCAACCACTGCGAGAATCCGCCCTGCGTGCCGGT
CTGCCCGGTCGGCGCGACGTTCAAGCGTGACGACGGCATCGTCGTCGTCGACGGCGACCGCTGCGTCGGCTGCGCCTACT
GCGTCCAGGCCTGCCCCTACGACGCGCGCTTCATCAACCACGAAACGAACAAGGCCGACAAGTGCACCTTCTGTGCGCAC
CGGGTCGACGCGGGCCTGCTGCCGGCCTGCGTCGAAACCTGCGTCGGCGGCGCCCGCGTCTTCGGCGATCTGAACGACGC
CAAGGGCGAATTGCGGCGCAGCCTCAACGCGGCGAAGAAGAACGGCGACGCCATCAAGGTGCTCAAGCCCGAAGCCGGCA
CCCGCCCCAATGTTTTCTACGTCGGTCTCGACGACCGGTTCCAGGGACGCGTCGAGGGCGAGGCAGCCCTCTGGCAACCC
ACTGCCCACGGAGTCTGA

Upstream 100 bases:

>100_bases
GACTACGAACAAGTACCTAGCGCGATAGAGAATCTACCCACTGTTGGAAGGGTCTTGGCGGGCTTAGTCTGTAATCGCAC
GCTCAACCAGGAGGCCGACC

Downstream 100 bases:

>100_bases
TCATGAACCCATCCATCGTCGAAGTCGTCAACGTGTCGCGCGAAGTCGCGTGGCTGCCCTGGGCCGTCCAGTATTTCTTC
CTGATCGGTCTCTCGGTCGG

Product: putative tetrathionate reductase subunit B

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSDNPSPSRRAFLQAIPAITAGLALPATARAAPHKHHWAMLVDVRKCIGCQACTVACIMENALPENSFRTLASTYVVQQE
TEGRTTAGTYMLPRLCNHCENPPCVPVCPVGATFKRDDGIVVVDGDRCVGCAYCVQACPYDARFINHETNKADKCTFCAH
RVDAGLLPACVETCVGGARVFGDLNDAKGELRRSLNAAKKNGDAIKVLKPEAGTRPNVFYVGLDDRFQGRVEGEAALWQP
TAHGV

Sequences:

>Translated_245_residues
MSDNPSPSRRAFLQAIPAITAGLALPATARAAPHKHHWAMLVDVRKCIGCQACTVACIMENALPENSFRTLASTYVVQQE
TEGRTTAGTYMLPRLCNHCENPPCVPVCPVGATFKRDDGIVVVDGDRCVGCAYCVQACPYDARFINHETNKADKCTFCAH
RVDAGLLPACVETCVGGARVFGDLNDAKGELRRSLNAAKKNGDAIKVLKPEAGTRPNVFYVGLDDRFQGRVEGEAALWQP
TAHGV
>Mature_244_residues
SDNPSPSRRAFLQAIPAITAGLALPATARAAPHKHHWAMLVDVRKCIGCQACTVACIMENALPENSFRTLASTYVVQQET
EGRTTAGTYMLPRLCNHCENPPCVPVCPVGATFKRDDGIVVVDGDRCVGCAYCVQACPYDARFINHETNKADKCTFCAHR
VDAGLLPACVETCVGGARVFGDLNDAKGELRRSLNAAKKNGDAIKVLKPEAGTRPNVFYVGLDDRFQGRVEGEAALWQPT
AHGV

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=223, Percent_Identity=39.0134529147982, Blast_Score=127, Evalue=7e-31,
Organism=Escherichia coli, GI226510944, Length=182, Percent_Identity=36.2637362637363, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1787122, Length=188, Percent_Identity=39.8936170212766, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1787872, Length=189, Percent_Identity=39.6825396825397, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1787749, Length=222, Percent_Identity=32.4324324324324, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787740, Length=111, Percent_Identity=45.945945945946, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787478, Length=99, Percent_Identity=49.4949494949495, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790326, Length=222, Percent_Identity=32.8828828828829, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789370, Length=219, Percent_Identity=33.7899543378995, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1789067, Length=181, Percent_Identity=32.0441988950276, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788811, Length=147, Percent_Identity=32.6530612244898, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI2367245, Length=164, Percent_Identity=32.3170731707317, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI87082180, Length=163, Percent_Identity=33.7423312883436, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082179, Length=139, Percent_Identity=33.0935251798561, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1787963, Length=134, Percent_Identity=30.5970149253731, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 26374; Mature: 26243

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
6.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNPSPSRRAFLQAIPAITAGLALPATARAAPHKHHWAMLVDVRKCIGCQACTVACIME
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
NALPENSFRTLASTYVVQQETEGRTTAGTYMLPRLCNHCENPPCVPVCPVGATFKRDDGI
HCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCEECCCCE
VVVDGDRCVGCAYCVQACPYDARFINHETNKADKCTFCAHRVDAGLLPACVETCVGGARV
EEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHE
FGDLNDAKGELRRSLNAAKKNGDAIKVLKPEAGTRPNVFYVGLDDRFQGRVEGEAALWQP
ECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCCEECCCEEEECC
TAHGV
CCCCC
>Mature Secondary Structure 
SDNPSPSRRAFLQAIPAITAGLALPATARAAPHKHHWAMLVDVRKCIGCQACTVACIME
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHH
NALPENSFRTLASTYVVQQETEGRTTAGTYMLPRLCNHCENPPCVPVCPVGATFKRDDGI
HCCCCHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCEECCCCE
VVVDGDRCVGCAYCVQACPYDARFINHETNKADKCTFCAHRVDAGLLPACVETCVGGARV
EEECCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHE
FGDLNDAKGELRRSLNAAKKNGDAIKVLKPEAGTRPNVFYVGLDDRFQGRVEGEAALWQP
ECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCCCEECCCEEEECC
TAHGV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]