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The map label for this gene is gltX

Identifier: 74317752

GI number: 74317752

Start: 1819182

End: 1820600

Strand: Direct

Name: gltX

Synonym: Tbd_1734

Alternate gene names: 74317752

Gene position: 1819182-1820600 (Clockwise)

Preceding gene: 74317748

Following gene: 74317757

Centisome position: 62.52

GC content: 67.02

Gene sequence:

>1419_bases
ATGGCGCCTTTTTCACGCCCGCATCACCCCATGATCCGCACCCGCTTCGCCCCCTCCCCCACCGGCTACCTGCACATCGG
CGGCGCGCGCACCGCGCTTTTCTCGTGGGCGTTCGCGCGTCATCACGGCGGGCAGTTCGTCCTGCGCATCGAGGACACCG
ACATCGCACGCTCGACGCCCGAGGCCGTGCAGGCGATCCTCGACGGCATGGCGTGGCTCGATCTCAATCACGACGAAGGC
CCGTTCTACCAGACCCAGCGCCTGTATCGCTACAAGGAAGTGATCGAGCAGATGCTGGCCAGCGGCGACGCCTATCACTG
CTACTGCAGTACCGAAGAGCTCGACGAAATGCGCGAGGCGCAACGCGCGCGCGGCGAAAAGCCCCGCTACGACGGACGCT
GGCGCCCCGAGCCCGGCAAGACCCTGCCGGTGCCGCCGTCGGGGGTGCAGCCCGTGGTGCGTTTCCGCAACCCGACCGAC
GGAACGGTGGCGTGGAAGGATCTCGTCAAGGGTACGATCGAGTTCTCCAACGCCGAACTCGACGACCTCATCATCGCGCG
CGCCGACGGCACGCCGACCTACAATTTCTGCGTCGTCGTCGACGACTGGGACATGCGGATCACGCACGTCATCCGCGGCG
ACGACCACGTCAACAACACGCCGCGCCAGATCAACATCCTCAAGGCGCTCGGCGCGACCGTCCCCGAGTATGCCCACCTG
TCGATGATCCTCGGCGACGACGGCACCAAGCTCTCCAAGCGCCACGGCGCGGTGTCGGTCATGCAGTACTTCGAGGAAGG
CTATCTGCCCGAGGCGGTGATCAACTATCTCGCCCGCCTCGGCTGGTCTCATGGCGACGCGGAACTGTTCGACCGCGAGC
AGTTCGTCGAGTGGTTCGACCTCGACCACATCACGCCGTCGGCCGCACAGTTCAACACCGAGAAGCTGCGCTGGCTCAAT
CAGCAGTACATCAAGGCGGCCGACGACACCCGTCTGGCCGGCCTCGCGCGTCCCTTTCTGATCCGCAACGGCGCGACGCT
CGACGATGGACCCGATCTCGCTGCGGTCTGTGCCCTGGTCAAGGAGCGGGCGGCAACGATCGAAGCGCTCGCCGACGCCG
CGACGCTCTTCTACCGGGTCCTCCACCCGACGCCCGAGCTGCTCGCCCAGCACGTCACGGCCGAGGTGCGGCCGGCGCTC
GCCGATCTCGCGACGCGCCTCGAAACGCTTGAATGGGAGCGTGGCGCGATCAGCGCGGCGTTCAAGGAGACGCTCGCCGC
GCATGGGCTGAAGATGCCCAAGCTCGCGATGCCGGTGCGCGTGCTCGTGACCGGCGAACCGCAGACGCCGGCGATCGACG
CGACACTCGAATTGCTCGGACGCGAAAAGGTCCTCGACCGCCTCAGGGCGGCGCTCTAG

Upstream 100 bases:

>100_bases
AAGGCGATGCTCTACCGCTGAGCTAACTGCCCCGAAGCGGCGCGAATTAAACCACACCCGCGCCGGCGAGGTCAATTTAG
CGGCGCGCCGATCCGGTAAA

Downstream 100 bases:

>100_bases
CTCGCCACAAGCGGAAACGAATCCGCTATAATGCGCGCTGTTTTTCGCCGGCGTAGCTCAGTTGGTAGAGCAGCGCATTC
GTAATGCGAAGGTCGTCAGT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAPFSRPHHPMIRTRFAPSPTGYLHIGGARTALFSWAFARHHGGQFVLRIEDTDIARSTPEAVQAILDGMAWLDLNHDEG
PFYQTQRLYRYKEVIEQMLASGDAYHCYCSTEELDEMREAQRARGEKPRYDGRWRPEPGKTLPVPPSGVQPVVRFRNPTD
GTVAWKDLVKGTIEFSNAELDDLIIARADGTPTYNFCVVVDDWDMRITHVIRGDDHVNNTPRQINILKALGATVPEYAHL
SMILGDDGTKLSKRHGAVSVMQYFEEGYLPEAVINYLARLGWSHGDAELFDREQFVEWFDLDHITPSAAQFNTEKLRWLN
QQYIKAADDTRLAGLARPFLIRNGATLDDGPDLAAVCALVKERAATIEALADAATLFYRVLHPTPELLAQHVTAEVRPAL
ADLATRLETLEWERGAISAAFKETLAAHGLKMPKLAMPVRVLVTGEPQTPAIDATLELLGREKVLDRLRAAL

Sequences:

>Translated_472_residues
MAPFSRPHHPMIRTRFAPSPTGYLHIGGARTALFSWAFARHHGGQFVLRIEDTDIARSTPEAVQAILDGMAWLDLNHDEG
PFYQTQRLYRYKEVIEQMLASGDAYHCYCSTEELDEMREAQRARGEKPRYDGRWRPEPGKTLPVPPSGVQPVVRFRNPTD
GTVAWKDLVKGTIEFSNAELDDLIIARADGTPTYNFCVVVDDWDMRITHVIRGDDHVNNTPRQINILKALGATVPEYAHL
SMILGDDGTKLSKRHGAVSVMQYFEEGYLPEAVINYLARLGWSHGDAELFDREQFVEWFDLDHITPSAAQFNTEKLRWLN
QQYIKAADDTRLAGLARPFLIRNGATLDDGPDLAAVCALVKERAATIEALADAATLFYRVLHPTPELLAQHVTAEVRPAL
ADLATRLETLEWERGAISAAFKETLAAHGLKMPKLAMPVRVLVTGEPQTPAIDATLELLGREKVLDRLRAAL
>Mature_471_residues
APFSRPHHPMIRTRFAPSPTGYLHIGGARTALFSWAFARHHGGQFVLRIEDTDIARSTPEAVQAILDGMAWLDLNHDEGP
FYQTQRLYRYKEVIEQMLASGDAYHCYCSTEELDEMREAQRARGEKPRYDGRWRPEPGKTLPVPPSGVQPVVRFRNPTDG
TVAWKDLVKGTIEFSNAELDDLIIARADGTPTYNFCVVVDDWDMRITHVIRGDDHVNNTPRQINILKALGATVPEYAHLS
MILGDDGTKLSKRHGAVSVMQYFEEGYLPEAVINYLARLGWSHGDAELFDREQFVEWFDLDHITPSAAQFNTEKLRWLNQ
QYIKAADDTRLAGLARPFLIRNGATLDDGPDLAAVCALVKERAATIEALADAATLFYRVLHPTPELLAQHVTAEVRPALA
DLATRLETLEWERGAISAAFKETLAAHGLKMPKLAMPVRVLVTGEPQTPAIDATLELLGREKVLDRLRAAL

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=329, Percent_Identity=35.5623100303951, Blast_Score=211, Evalue=9e-55,
Organism=Escherichia coli, GI1788743, Length=463, Percent_Identity=54.2116630669546, Blast_Score=508, Evalue=1e-145,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=33.8775510204082, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1786895, Length=125, Percent_Identity=28, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=31.2368972746331, Blast_Score=214, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6324540, Length=337, Percent_Identity=36.7952522255193, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6324742, Length=287, Percent_Identity=27.5261324041812, Blast_Score=80, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6321192, Length=262, Percent_Identity=27.0992366412214, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24665321, Length=498, Percent_Identity=30.9236947791165, Blast_Score=237, Evalue=1e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_THIDA (Q3SI46)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315492.1
- ProteinModelPortal:   Q3SI46
- SMR:   Q3SI46
- STRING:   Q3SI46
- GeneID:   3671318
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1734
- NMPDR:   fig|292415.3.peg.1831
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q3SI46
- ProtClustDB:   PRK01406
- BioCyc:   TDEN292415:TBD_1734-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52880; Mature: 52748

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPFSRPHHPMIRTRFAPSPTGYLHIGGARTALFSWAFARHHGGQFVLRIEDTDIARSTP
CCCCCCCCCCHHEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCH
EAVQAILDGMAWLDLNHDEGPFYQTQRLYRYKEVIEQMLASGDAYHCYCSTEELDEMREA
HHHHHHHCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH
QRARGEKPRYDGRWRPEPGKTLPVPPSGVQPVVRFRNPTDGTVAWKDLVKGTIEFSNAEL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHHHHHCCCCCCC
DDLIIARADGTPTYNFCVVVDDWDMRITHVIRGDDHVNNTPRQINILKALGATVPEYAHL
CCEEEEECCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCHHEE
SMILGDDGTKLSKRHGAVSVMQYFEEGYLPEAVINYLARLGWSHGDAELFDREQFVEWFD
EEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
LDHITPSAAQFNTEKLRWLNQQYIKAADDTRLAGLARPFLIRNGATLDDGPDLAAVCALV
CCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCCEEECCCCCCCCCCHHHHHHHH
KERAATIEALADAATLFYRVLHPTPELLAQHVTAEVRPALADLATRLETLEWERGAISAA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FKETLAAHGLKMPKLAMPVRVLVTGEPQTPAIDATLELLGREKVLDRLRAAL
HHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
APFSRPHHPMIRTRFAPSPTGYLHIGGARTALFSWAFARHHGGQFVLRIEDTDIARSTP
CCCCCCCCCHHEEECCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCH
EAVQAILDGMAWLDLNHDEGPFYQTQRLYRYKEVIEQMLASGDAYHCYCSTEELDEMREA
HHHHHHHCCHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHH
QRARGEKPRYDGRWRPEPGKTLPVPPSGVQPVVRFRNPTDGTVAWKDLVKGTIEFSNAEL
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHHHHHCCCCCCC
DDLIIARADGTPTYNFCVVVDDWDMRITHVIRGDDHVNNTPRQINILKALGATVPEYAHL
CCEEEEECCCCCCEEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCHHEE
SMILGDDGTKLSKRHGAVSVMQYFEEGYLPEAVINYLARLGWSHGDAELFDREQFVEWFD
EEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHC
LDHITPSAAQFNTEKLRWLNQQYIKAADDTRLAGLARPFLIRNGATLDDGPDLAAVCALV
CCCCCCCHHHCCHHHHHHHHHHHHHHCCCCHHHHHHCCCEEECCCCCCCCCCHHHHHHHH
KERAATIEALADAATLFYRVLHPTPELLAQHVTAEVRPALADLATRLETLEWERGAISAA
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FKETLAAHGLKMPKLAMPVRVLVTGEPQTPAIDATLELLGREKVLDRLRAAL
HHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA