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The map label for this gene is rnhA

Identifier: 74317681

GI number: 74317681

Start: 1747503

End: 1747949

Strand: Reverse

Name: rnhA

Synonym: Tbd_1663

Alternate gene names: 74317681

Gene position: 1747949-1747503 (Counterclockwise)

Preceding gene: 74317682

Following gene: 74317680

Centisome position: 60.07

GC content: 63.76

Gene sequence:

>447_bases
GTGACCGCTGACATCATTTATATATATAGCGACGGTGCCTGCAAGGGCAATCCCGGCGCAGGCGGCTGGGGCGCGCTTCT
CGTCGCGGGCGGCCACCGCAAGGAGATAAGCGGCGGCGAGCCGAACACGACCAATAACCGCATGGAGATGACCGCGGTCA
TCCGCGCGCTCGAGCTGCTCAAGCGGCCTTCGACGGTCGAGGTCCACACCGACTCGCAGTACGTGCAGAAAGGGGTCAGC
GAATGGCTGCCCGGGTGGAAACGGCGCAACTGGCGGACGGCCGACGGCAAGCCGGTCAAGAATCAGGACCTCTGGCAACA
GCTCGATGCACTGAGCCAGCAGCATCGCATCGTATGGAAGTGGGTGCGCGGCCACGCCGGTCATCCCGAGAACGAACGCG
CCGACGTACTGGCCAATCAGGGCGTCTTGCAGGCTCGGCAATATTGA

Upstream 100 bases:

>100_bases
GGAATGAACATCATCCAGCCGCAGTGGTCGACGCCCGTGGCACGGCGCCTGCTCTCGCCCGCGGCCAAGGTGATCAATCT
CAAACACTACCGAATACATC

Downstream 100 bases:

>100_bases
TCGAAAGACATGCGTCAAATCATCCTCGATACGGAAACGACCGGGCTCGACCCGGCCCAGGGCCATCGCATCATCGAAGT
CGCGGCGGTGGAAATGGTCA

Product: ribonuclease HI

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MTADIIYIYSDGACKGNPGAGGWGALLVAGGHRKEISGGEPNTTNNRMEMTAVIRALELLKRPSTVEVHTDSQYVQKGVS
EWLPGWKRRNWRTADGKPVKNQDLWQQLDALSQQHRIVWKWVRGHAGHPENERADVLANQGVLQARQY

Sequences:

>Translated_148_residues
MTADIIYIYSDGACKGNPGAGGWGALLVAGGHRKEISGGEPNTTNNRMEMTAVIRALELLKRPSTVEVHTDSQYVQKGVS
EWLPGWKRRNWRTADGKPVKNQDLWQQLDALSQQHRIVWKWVRGHAGHPENERADVLANQGVLQARQY
>Mature_147_residues
TADIIYIYSDGACKGNPGAGGWGALLVAGGHRKEISGGEPNTTNNRMEMTAVIRALELLKRPSTVEVHTDSQYVQKGVSE
WLPGWKRRNWRTADGKPVKNQDLWQQLDALSQQHRIVWKWVRGHAGHPENERADVLANQGVLQARQY

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=152, Percent_Identity=34.8684210526316, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1786408, Length=138, Percent_Identity=56.5217391304348, Blast_Score=167, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI115532542, Length=144, Percent_Identity=37.5, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17505482, Length=130, Percent_Identity=34.6153846153846, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45552509, Length=149, Percent_Identity=34.2281879194631, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137476, Length=149, Percent_Identity=34.2281879194631, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_THIDA (Q3SIB2)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315421.1
- HSSP:   P0A7Y4
- ProteinModelPortal:   Q3SIB2
- STRING:   Q3SIB2
- GeneID:   3673048
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1663
- NMPDR:   fig|292415.3.peg.1760
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- PhylomeDB:   Q3SIB2
- BioCyc:   TDEN292415:TBD_1663-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 16597; Mature: 16466

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADIIYIYSDGACKGNPGAGGWGALLVAGGHRKEISGGEPNTTNNRMEMTAVIRALELL
CCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KRPSTVEVHTDSQYVQKGVSEWLPGWKRRNWRTADGKPVKNQDLWQQLDALSQQHRIVWK
CCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
WVRGHAGHPENERADVLANQGVLQARQY
HHHCCCCCCCCCCHHHHHCCCCHHHCCC
>Mature Secondary Structure 
TADIIYIYSDGACKGNPGAGGWGALLVAGGHRKEISGGEPNTTNNRMEMTAVIRALELL
CCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KRPSTVEVHTDSQYVQKGVSEWLPGWKRRNWRTADGKPVKNQDLWQQLDALSQQHRIVWK
CCCCEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
WVRGHAGHPENERADVLANQGVLQARQY
HHHCCCCCCCCCCHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA