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The map label for this gene is ecaIM [H]

Identifier: 74317509

GI number: 74317509

Start: 1578754

End: 1581093

Strand: Reverse

Name: ecaIM [H]

Synonym: Tbd_1491

Alternate gene names: 74317509

Gene position: 1581093-1578754 (Counterclockwise)

Preceding gene: 74317510

Following gene: 74317508

Centisome position: 54.34

GC content: 56.54

Gene sequence:

>2340_bases
ATGGCCCGCCAGCCCAAGGAAGCCTACGACCTGACGGACGCCGAAAAGCGCGATCTCATACAGTTGATTCAGGAAGGCAA
AGCCTTGCCCGAGAAATACCGCTTCATCCTGTTCGAGGACAAGCGCGAAGTGGAACTGGTGTGGAACGGCAAGAGCCGCG
AGGTCTGCACCACCGTGCTGCCATTTCAGTCGCTGGAGCATGTGGACGAGCCGCGTCTGGAAAAACCGGAACTCGGTACC
ATGGACCTGTTCGACACCCGCGGCCGCCAGCTTAAGGGCTGGACCAACAAGCTCATCTGGGGTGATAACAAACTAATTCT
TTCATCGCTTAAGAGCGGCGCGCTACGCCAGCAGATCGAGGATGCAGGTGGCTTGAAGCTGATTTACATCGACCCGCCCT
TCGATGTGGGAGCCGATTTCTCTATGGACATCGAGATCGGCGGCGAGACCTTCCACAAGGAGCCCAACCTGCTGGAACAG
ATAGCCTACCGCGACACTTGGGGGCGCGGGGCGGATTCCTTTATCTCGATGGTCTATGAGCGCTTGATTTTGATGCGGGA
TTTGCTGGCGGACGATGGCTCAATATATGTACACATGGGAAAGACTGTTGCTCACTATGTCAGGCTTGCTTGTGAGGAGG
TGTTTGGATCAGCGAACATCGTCAATGAGATTACGTGGAAGCGCTCTCATGCACACGGAGATACTGGCCAGGGGGCGTCC
CATTTTGGAAGGACTACCGAGACAATTCTTCTTTTCCGGAAGAGCGAAAAAGGGATATGGAATCCACAACACAAACCCTA
CACGGATGACGTGCTCGCTAGGGATTACAAATACACGGAAGAAAAGACTGGCGAGCGGTATCGTCTGATGCCAGTCGATG
GCCCTGGCGGGGCGGCAAAAGGTAACCCTTATTACGATTTTCTGGGCGTTAAGGGCTATTGGCGGTATTCATTTGAAACA
ATGACCAAGCTTTATGAGCAGGGTGAGATCGTACTTTCTTCAACCGGGAAGAGTCTTAGTCGTAAGAAATTCCTCAAAGA
TGCGGAAGGCACGCCGGTAGTCGATTTATGGGACGAAGTGAACCGCATCAGTCCAACTTCGTCGGAGAGACTAGACTACC
CCACCCAAAAACCAGAAGCTCTGCTCGAACGTATTATCAAAGCCAGTAGCAACGAAGGTGATTTGGTGGCGGACTTTTTT
TGTGGCAGTGGTACGACCGCTGCAGTGGCAGAAAAGCTAGGCCGCAAGTGGATAGCAACCGATCTCGGCAAGTTTGGTGT
TCACACCACGCGCAAGCGCATGATTGGGGTGCAGCGGCAACTGAAGGACTCCGGCAAGCCGTTCCGCGCCTTCGAGGTGT
TGAACCTGGGTCGGTATGAGCGCCAGGCTTATCTCAATGTTGGTGGGCGCTTGAACGGCCATCAGAAGGAACAGCTACTG
GCACAAAAAGAGCGCGAGTTCCGTGATCTCATCCTGCGCGCCTACAAGGCCCAGCCGCTGGAGGGCGAAAGCTTCTTCCA
TGGCAAGAACGCGGGGCGGCTGGTCGTGATCGGCCCCATCAACCTGCCCGTGGGCCGGCTGTTCGTGGAGGAAGTCATCA
CCGAGTGTCGCAAGCGCGGCGCCTCGCGCGTGGACATGCTCGCCTTCGAGTTCGAGATGGGCCTGTTCCCGGCAGTGCTG
GAGGAGGCCAAGCAGAAGGGTATCGACCTGGTGCCAAAGCAGATTCCGCCCGAAGTGTTCGACAAGCGCGCGGTCGAAAA
AGGCCAGGTGGTGTTCTTCGATATCAGCTTCGTCGAGGCCACGCCGCGCTACGCCAAGAAAGACAAGTTCACGCTGCAGA
TCGAGCTGACGGATTTTTCCGTCTACTACACCCAGGGCGCGGCCGAGGCCGCTGCCGCTAGCCTGAAAAATGGCAAGAGC
CAGGTTGTTTGCGAACAAGGCCAGCTTATCAAGTTGAGCAAGGACAAGGACGGCGTCGTCACCCGCGAGATTCTTACCAA
GAACTGGACCGACTGGGTGGACTACTGGGCGGTGGACTTCGACTACATGAGCCGCAAGGAAATCATCAAGGTCGCCAAGG
GCGCAGGCATTGACGGCAGCCTTCCCGGCTTCGAGCCGCCGCAGGGCGAGTTGACCCTGCCGGAGTTCGAGGAACGCTGG
ACCGGCGGCTACATCTTTGAAAACGAGTGGCAGAGCTTCCGCACCCGCAAGAGTCGCGACCTGGAAATGATTTCCGCGCC
GCACACCTACACCCAGGCTGGCCGCTATACCGTGGCCGTAAAGGTGATTGATATCTTCGGCAACGACACCATGGTGCTGG
TGCCGGTGAGCGTGGGCTAA

Upstream 100 bases:

>100_bases
ACCGCTTCGTATACGTGGACCAACAAGGCTATGAGCGCAACCCGCCTAAGACCTTCGCCGCGCTCGCCGCGAGCTTTACC
GAATTTCAGGAGATCTGAAC

Downstream 100 bases:

>100_bases
GCGCCATGGCGGTGCCAAATCCTGTCCGCCTGTTTCACATCACGGCAATTGCCAATTTGCCGGCAATTTGTGCGGCAGGC
GCGTTGCTGTCCAAGAATCA

Product: adenine specific DNA-methyltransferase

Products: NA

Alternate protein names: M.EcaI; Adenine-specific methyltransferase EcaI [H]

Number of amino acids: Translated: 779; Mature: 778

Protein sequence:

>779_residues
MARQPKEAYDLTDAEKRDLIQLIQEGKALPEKYRFILFEDKREVELVWNGKSREVCTTVLPFQSLEHVDEPRLEKPELGT
MDLFDTRGRQLKGWTNKLIWGDNKLILSSLKSGALRQQIEDAGGLKLIYIDPPFDVGADFSMDIEIGGETFHKEPNLLEQ
IAYRDTWGRGADSFISMVYERLILMRDLLADDGSIYVHMGKTVAHYVRLACEEVFGSANIVNEITWKRSHAHGDTGQGAS
HFGRTTETILLFRKSEKGIWNPQHKPYTDDVLARDYKYTEEKTGERYRLMPVDGPGGAAKGNPYYDFLGVKGYWRYSFET
MTKLYEQGEIVLSSTGKSLSRKKFLKDAEGTPVVDLWDEVNRISPTSSERLDYPTQKPEALLERIIKASSNEGDLVADFF
CGSGTTAAVAEKLGRKWIATDLGKFGVHTTRKRMIGVQRQLKDSGKPFRAFEVLNLGRYERQAYLNVGGRLNGHQKEQLL
AQKEREFRDLILRAYKAQPLEGESFFHGKNAGRLVVIGPINLPVGRLFVEEVITECRKRGASRVDMLAFEFEMGLFPAVL
EEAKQKGIDLVPKQIPPEVFDKRAVEKGQVVFFDISFVEATPRYAKKDKFTLQIELTDFSVYYTQGAAEAAAASLKNGKS
QVVCEQGQLIKLSKDKDGVVTREILTKNWTDWVDYWAVDFDYMSRKEIIKVAKGAGIDGSLPGFEPPQGELTLPEFEERW
TGGYIFENEWQSFRTRKSRDLEMISAPHTYTQAGRYTVAVKVIDIFGNDTMVLVPVSVG

Sequences:

>Translated_779_residues
MARQPKEAYDLTDAEKRDLIQLIQEGKALPEKYRFILFEDKREVELVWNGKSREVCTTVLPFQSLEHVDEPRLEKPELGT
MDLFDTRGRQLKGWTNKLIWGDNKLILSSLKSGALRQQIEDAGGLKLIYIDPPFDVGADFSMDIEIGGETFHKEPNLLEQ
IAYRDTWGRGADSFISMVYERLILMRDLLADDGSIYVHMGKTVAHYVRLACEEVFGSANIVNEITWKRSHAHGDTGQGAS
HFGRTTETILLFRKSEKGIWNPQHKPYTDDVLARDYKYTEEKTGERYRLMPVDGPGGAAKGNPYYDFLGVKGYWRYSFET
MTKLYEQGEIVLSSTGKSLSRKKFLKDAEGTPVVDLWDEVNRISPTSSERLDYPTQKPEALLERIIKASSNEGDLVADFF
CGSGTTAAVAEKLGRKWIATDLGKFGVHTTRKRMIGVQRQLKDSGKPFRAFEVLNLGRYERQAYLNVGGRLNGHQKEQLL
AQKEREFRDLILRAYKAQPLEGESFFHGKNAGRLVVIGPINLPVGRLFVEEVITECRKRGASRVDMLAFEFEMGLFPAVL
EEAKQKGIDLVPKQIPPEVFDKRAVEKGQVVFFDISFVEATPRYAKKDKFTLQIELTDFSVYYTQGAAEAAAASLKNGKS
QVVCEQGQLIKLSKDKDGVVTREILTKNWTDWVDYWAVDFDYMSRKEIIKVAKGAGIDGSLPGFEPPQGELTLPEFEERW
TGGYIFENEWQSFRTRKSRDLEMISAPHTYTQAGRYTVAVKVIDIFGNDTMVLVPVSVG
>Mature_778_residues
ARQPKEAYDLTDAEKRDLIQLIQEGKALPEKYRFILFEDKREVELVWNGKSREVCTTVLPFQSLEHVDEPRLEKPELGTM
DLFDTRGRQLKGWTNKLIWGDNKLILSSLKSGALRQQIEDAGGLKLIYIDPPFDVGADFSMDIEIGGETFHKEPNLLEQI
AYRDTWGRGADSFISMVYERLILMRDLLADDGSIYVHMGKTVAHYVRLACEEVFGSANIVNEITWKRSHAHGDTGQGASH
FGRTTETILLFRKSEKGIWNPQHKPYTDDVLARDYKYTEEKTGERYRLMPVDGPGGAAKGNPYYDFLGVKGYWRYSFETM
TKLYEQGEIVLSSTGKSLSRKKFLKDAEGTPVVDLWDEVNRISPTSSERLDYPTQKPEALLERIIKASSNEGDLVADFFC
GSGTTAAVAEKLGRKWIATDLGKFGVHTTRKRMIGVQRQLKDSGKPFRAFEVLNLGRYERQAYLNVGGRLNGHQKEQLLA
QKEREFRDLILRAYKAQPLEGESFFHGKNAGRLVVIGPINLPVGRLFVEEVITECRKRGASRVDMLAFEFEMGLFPAVLE
EAKQKGIDLVPKQIPPEVFDKRAVEKGQVVFFDISFVEATPRYAKKDKFTLQIELTDFSVYYTQGAAEAAAASLKNGKSQ
VVCEQGQLIKLSKDKDGVVTREILTKNWTDWVDYWAVDFDYMSRKEIIKVAKGAGIDGSLPGFEPPQGELTLPEFEERWT
GGYIFENEWQSFRTRKSRDLEMISAPHTYTQAGRYTVAVKVIDIFGNDTMVLVPVSVG

Specific function: This methylase recognizes the double-stranded sequence GGTNACC, causes specific methylation on A-5 on both strands and protects the DNA from cleavage by the EcaI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=80, Percent_Identity=46.25, Blast_Score=66, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 88357; Mature: 88225

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00092 N6_MTASE ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQPKEAYDLTDAEKRDLIQLIQEGKALPEKYRFILFEDKREVELVWNGKSREVCTTVL
CCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHH
PFQSLEHVDEPRLEKPELGTMDLFDTRGRQLKGWTNKLIWGDNKLILSSLKSGALRQQIE
CHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHH
DAGGLKLIYIDPPFDVGADFSMDIEIGGETFHKEPNLLEQIAYRDTWGRGADSFISMVYE
HCCCEEEEEECCCCCCCCCEEEEEEECCHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
RLILMRDLLADDGSIYVHMGKTVAHYVRLACEEVFGSANIVNEITWKRSHAHGDTGQGAS
HHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHH
HFGRTTETILLFRKSEKGIWNPQHKPYTDDVLARDYKYTEEKTGERYRLMPVDGPGGAAK
HCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCHHCCCCEEEEEEECCCCCCCC
GNPYYDFLGVKGYWRYSFETMTKLYEQGEIVLSSTGKSLSRKKFLKDAEGTPVVDLWDEV
CCCCEEEECCCEEEEEHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCEEHHHHHH
NRISPTSSERLDYPTQKPEALLERIIKASSNEGDLVADFFCGSGTTAAVAEKLGRKWIAT
HCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHH
DLGKFGVHTTRKRMIGVQRQLKDSGKPFRAFEVLNLGRYERQAYLNVGGRLNGHQKEQLL
HHHHHCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCHHHHEEEECCCCCCCHHHHHHH
AQKEREFRDLILRAYKAQPLEGESFFHGKNAGRLVVIGPINLPVGRLFVEEVITECRKRG
HHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
ASRVDMLAFEFEMGLFPAVLEEAKQKGIDLVPKQIPPEVFDKRAVEKGQVVFFDISFVEA
CCHHEEEEEEHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEEEC
TPRYAKKDKFTLQIELTDFSVYYTQGAAEAAAASLKNGKSQVVCEQGQLIKLSKDKDGVV
CCCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCH
TREILTKNWTDWVDYWAVDFDYMSRKEIIKVAKGAGIDGSLPGFEPPQGELTLPEFEERW
HHHHHHCCCHHHHHHHEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHC
TGGYIFENEWQSFRTRKSRDLEMISAPHTYTQAGRYTVAVKVIDIFGNDTMVLVPVSVG
CCCEEECCCHHHHHHHCCCCCEEEECCCCHHCCCCEEEEEEEEEEECCCEEEEEEECCC
>Mature Secondary Structure 
ARQPKEAYDLTDAEKRDLIQLIQEGKALPEKYRFILFEDKREVELVWNGKSREVCTTVL
CCCCCHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEEEECCCEEEEEECCCCCCHHHHHH
PFQSLEHVDEPRLEKPELGTMDLFDTRGRQLKGWTNKLIWGDNKLILSSLKSGALRQQIE
CHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHH
DAGGLKLIYIDPPFDVGADFSMDIEIGGETFHKEPNLLEQIAYRDTWGRGADSFISMVYE
HCCCEEEEEECCCCCCCCCEEEEEEECCHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
RLILMRDLLADDGSIYVHMGKTVAHYVRLACEEVFGSANIVNEITWKRSHAHGDTGQGAS
HHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCHH
HFGRTTETILLFRKSEKGIWNPQHKPYTDDVLARDYKYTEEKTGERYRLMPVDGPGGAAK
HCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHHCCCCCCHHCCCCEEEEEEECCCCCCCC
GNPYYDFLGVKGYWRYSFETMTKLYEQGEIVLSSTGKSLSRKKFLKDAEGTPVVDLWDEV
CCCCEEEECCCEEEEEHHHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCEEHHHHHH
NRISPTSSERLDYPTQKPEALLERIIKASSNEGDLVADFFCGSGTTAAVAEKLGRKWIAT
HCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHCCHHHHH
DLGKFGVHTTRKRMIGVQRQLKDSGKPFRAFEVLNLGRYERQAYLNVGGRLNGHQKEQLL
HHHHHCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHCCCHHHHEEEECCCCCCCHHHHHHH
AQKEREFRDLILRAYKAQPLEGESFFHGKNAGRLVVIGPINLPVGRLFVEEVITECRKRG
HHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCC
ASRVDMLAFEFEMGLFPAVLEEAKQKGIDLVPKQIPPEVFDKRAVEKGQVVFFDISFVEA
CCHHEEEEEEHHHCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEEEEEEEEEC
TPRYAKKDKFTLQIELTDFSVYYTQGAAEAAAASLKNGKSQVVCEQGQLIKLSKDKDGVV
CCCCCCCCCEEEEEEEEEEEEEEECCHHHHHHHHHHCCCCEEEECCCCEEEECCCCCCCH
TREILTKNWTDWVDYWAVDFDYMSRKEIIKVAKGAGIDGSLPGFEPPQGELTLPEFEERW
HHHHHHCCCHHHHHHHEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHC
TGGYIFENEWQSFRTRKSRDLEMISAPHTYTQAGRYTVAVKVIDIFGNDTMVLVPVSVG
CCCEEECCCHHHHHHHCCCCCEEEECCCCHHCCCCEEEEEEEEEEECCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2183182 [H]