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The map label for this gene is epsE [H]

Identifier: 74317452

GI number: 74317452

Start: 1522373

End: 1524049

Strand: Direct

Name: epsE [H]

Synonym: Tbd_1434

Alternate gene names: 74317452

Gene position: 1522373-1524049 (Clockwise)

Preceding gene: 74317451

Following gene: 74317453

Centisome position: 52.32

GC content: 69.17

Gene sequence:

>1677_bases
ATGAACGCGCCGCAAACGCCGCTCAGCGCCGACGTGCTCGCCGGCTTGCGTCGCGAGGCGCAGGCGGCCGGCCAGAGCAT
CGTGCTGCGCGTGCAGGAGCAGTTCGGCCTCGACGCCGACACGGCCTTGCGCATGGTCGCCGCGCAGACCCGGTTGCCGG
CCTTCGACATGGCGGCACTGAATGCGCTCACCCCCGACTTCAACCGCCTGGGCTTCAGCGAGGCCGCACGGCGGCATTGC
GTCGCGGCACGCGACGGGACGTCGAAGCTCTGGCTGCTGCTGACCGATCCCTGGGACAGCGCGGCCTCGGCGTACGCCGC
GCACGTGCTCGGCGAGCCTTTCCGGACCGGCCTTGCGCACCCCGCCGATCTCGCCGCGCTGCTCGCTCGCCACGAAGACG
GCCTGCGCGCGATGGAGGGCGTGGGCGGCGGCAAAGTCCAGAACGCCGCCGACCCCGGCATCGAGTCGCTGTCTTTCGCC
AGCATCGCCGAGGACGCGAGCCCGGTCGTGCGGCTCGTGCGCTCGACGCTCTACGACGCGCTCAAGGCCGGCGCGTCGGA
CATCCACCTCGAGACCGACGCGCGCGGGCTCACGGTCAAATACCGCATCGACGGCGTACTCTCGCACGTCGCGCAGATCG
CCGGAACAGACGTCGCCGAGCAGGCGGTCTCGCGGATCAAGGTCATGTCCGAGCTCGACATCGCCGAGCGCCGCGTGCCG
CAGGACGGCCGTTTCAAGGTCGCGATGCAAGGCCGCGAAGTCGACGTCCGCGTGTCGGTGATGCCGAGCGTGTTCGGCGA
GGACGCGGTACTGCGCATCCTCGACCGTCAGGCGCTGTCCGAGGAACTGGCGGGCTTGACCCTCGAAGGCCTCGGCTACG
CCGGGACCTCGCTCGCCCGAATTCGCAAGCTCGCGGCCGAGCCCTACGGCATGCTGCTCGTCACCGGCCCGACGGGATCC
GGAAAGACGACGACCCTGTATGCCGCGATTTCGGAGATCAACCACGGCCGCGACAAGATCGTCACGATCGAGGACCCCGT
CGAATACCAGCTCCCGGGCGTCCTGCAGATTCCGGTCAACGAGAAAAAGGGGCTCAGCTTCGCGCGCGGCCTGCGCTCGA
TCCTGCGCCACGATCCCGACAAGATCATGGTCGGTGAAATCCGCGACGCCGACACGGCCCAGATCGCCGTCCAGTCGGCG
CTTACCGGGCACCTCGTGTTCACGACCGTGCACGCGAACAACGTCTTCGACGTCATCGGCCGTTTCATCCACATGGGGGT
CGACCCCTACAGCTTCGTCTCGGCGCTCAACGGCATCGTCGCGCAACGGCTCGTGCGCATGACTTGCCAGCACTGCGCGC
AAGCCGCGACGCCGAACGCGGAACTGCTCGAGCTGTCCGGGCTCAGTGTCGACGACACGCGCGACTACAACTTCCGCCAG
GGCGCGGGCTGCGGCCATTGCCGCGGCAGCGGCTACAAGGGCCGCCGAGCGGTCGCCGAGATCCTGCTCCTCACCGACAC
CCTGCGCGAACTCGTCGTCGCCCGCGCCCCGATCGGCCAGATCAAGGAAGCCGCGACACGCGGCGGCACGCGCAACCTGC
GCGACGAAGCCCTCGCGCTGGTGAAGGCCGGCGACACCACCCTCGAAGAGATCAACCGTGTCACGCTGGTCGGCTGA

Upstream 100 bases:

>100_bases
GGCTCTTCGAACCGCTTTTGATGGTGTTCATCGGGCTCGTCATCGGGTCGATCGTGGTCCTGCTCTACATGCCTATTTTC
GAACTCGCGGGGTCGCTCGA

Downstream 100 bases:

>100_bases
ACGGCTTCGCATCGGACTCTCCCCGGGCGAAGCAGCCTTGCGGCGCGGCGCGCAAACCCTGCGGCTGCGCGCCGCGGAAC
GCGGACCGGGCGCGCTGCTC

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 558; Mature: 558

Protein sequence:

>558_residues
MNAPQTPLSADVLAGLRREAQAAGQSIVLRVQEQFGLDADTALRMVAAQTRLPAFDMAALNALTPDFNRLGFSEAARRHC
VAARDGTSKLWLLLTDPWDSAASAYAAHVLGEPFRTGLAHPADLAALLARHEDGLRAMEGVGGGKVQNAADPGIESLSFA
SIAEDASPVVRLVRSTLYDALKAGASDIHLETDARGLTVKYRIDGVLSHVAQIAGTDVAEQAVSRIKVMSELDIAERRVP
QDGRFKVAMQGREVDVRVSVMPSVFGEDAVLRILDRQALSEELAGLTLEGLGYAGTSLARIRKLAAEPYGMLLVTGPTGS
GKTTTLYAAISEINHGRDKIVTIEDPVEYQLPGVLQIPVNEKKGLSFARGLRSILRHDPDKIMVGEIRDADTAQIAVQSA
LTGHLVFTTVHANNVFDVIGRFIHMGVDPYSFVSALNGIVAQRLVRMTCQHCAQAATPNAELLELSGLSVDDTRDYNFRQ
GAGCGHCRGSGYKGRRAVAEILLLTDTLRELVVARAPIGQIKEAATRGGTRNLRDEALALVKAGDTTLEEINRVTLVG

Sequences:

>Translated_558_residues
MNAPQTPLSADVLAGLRREAQAAGQSIVLRVQEQFGLDADTALRMVAAQTRLPAFDMAALNALTPDFNRLGFSEAARRHC
VAARDGTSKLWLLLTDPWDSAASAYAAHVLGEPFRTGLAHPADLAALLARHEDGLRAMEGVGGGKVQNAADPGIESLSFA
SIAEDASPVVRLVRSTLYDALKAGASDIHLETDARGLTVKYRIDGVLSHVAQIAGTDVAEQAVSRIKVMSELDIAERRVP
QDGRFKVAMQGREVDVRVSVMPSVFGEDAVLRILDRQALSEELAGLTLEGLGYAGTSLARIRKLAAEPYGMLLVTGPTGS
GKTTTLYAAISEINHGRDKIVTIEDPVEYQLPGVLQIPVNEKKGLSFARGLRSILRHDPDKIMVGEIRDADTAQIAVQSA
LTGHLVFTTVHANNVFDVIGRFIHMGVDPYSFVSALNGIVAQRLVRMTCQHCAQAATPNAELLELSGLSVDDTRDYNFRQ
GAGCGHCRGSGYKGRRAVAEILLLTDTLRELVVARAPIGQIKEAATRGGTRNLRDEALALVKAGDTTLEEINRVTLVG
>Mature_558_residues
MNAPQTPLSADVLAGLRREAQAAGQSIVLRVQEQFGLDADTALRMVAAQTRLPAFDMAALNALTPDFNRLGFSEAARRHC
VAARDGTSKLWLLLTDPWDSAASAYAAHVLGEPFRTGLAHPADLAALLARHEDGLRAMEGVGGGKVQNAADPGIESLSFA
SIAEDASPVVRLVRSTLYDALKAGASDIHLETDARGLTVKYRIDGVLSHVAQIAGTDVAEQAVSRIKVMSELDIAERRVP
QDGRFKVAMQGREVDVRVSVMPSVFGEDAVLRILDRQALSEELAGLTLEGLGYAGTSLARIRKLAAEPYGMLLVTGPTGS
GKTTTLYAAISEINHGRDKIVTIEDPVEYQLPGVLQIPVNEKKGLSFARGLRSILRHDPDKIMVGEIRDADTAQIAVQSA
LTGHLVFTTVHANNVFDVIGRFIHMGVDPYSFVSALNGIVAQRLVRMTCQHCAQAATPNAELLELSGLSVDDTRDYNFRQ
GAGCGHCRGSGYKGRRAVAEILLLTDTLRELVVARAPIGQIKEAATRGGTRNLRDEALALVKAGDTTLEEINRVTLVG

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=441, Percent_Identity=42.1768707482993, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI1786296, Length=398, Percent_Identity=38.1909547738693, Blast_Score=265, Evalue=8e-72,
Organism=Escherichia coli, GI87082188, Length=167, Percent_Identity=40.1197604790419, Blast_Score=98, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 59757; Mature: 59757

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00687 ALDEHYDE_DEHYDR_GLU ; PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPQTPLSADVLAGLRREAQAAGQSIVLRVQEQFGLDADTALRMVAAQTRLPAFDMAAL
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHHHHHCCCHHHHHHH
NALTPDFNRLGFSEAARRHCVAARDGTSKLWLLLTDPWDSAASAYAAHVLGEPFRTGLAH
HHCCCCHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHCCCC
PADLAALLARHEDGLRAMEGVGGGKVQNAADPGIESLSFASIAEDASPVVRLVRSTLYDA
HHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LKAGASDIHLETDARGLTVKYRIDGVLSHVAQIAGTDVAEQAVSRIKVMSELDIAERRVP
HHCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
QDGRFKVAMQGREVDVRVSVMPSVFGEDAVLRILDRQALSEELAGLTLEGLGYAGTSLAR
CCCCEEEEEECCEEEEEEEECCHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
IRKLAAEPYGMLLVTGPTGSGKTTTLYAAISEINHGRDKIVTIEDPVEYQLPGVLQIPVN
HHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCC
EKKGLSFARGLRSILRHDPDKIMVGEIRDADTAQIAVQSALTGHLVFTTVHANNVFDVIG
CCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEEEECCCHHHHHH
RFIHMGVDPYSFVSALNGIVAQRLVRMTCQHCAQAATPNAELLELSGLSVDDTRDYNFRQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCC
GAGCGHCRGSGYKGRRAVAEILLLTDTLRELVVARAPIGQIKEAATRGGTRNLRDEALAL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHH
VKAGDTTLEEINRVTLVG
HHCCCHHHHHCCEEEECC
>Mature Secondary Structure
MNAPQTPLSADVLAGLRREAQAAGQSIVLRVQEQFGLDADTALRMVAAQTRLPAFDMAAL
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHHHHHHHHHHHCCCHHHHHHH
NALTPDFNRLGFSEAARRHCVAARDGTSKLWLLLTDPWDSAASAYAAHVLGEPFRTGLAH
HHCCCCHHHCCHHHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHCCCC
PADLAALLARHEDGLRAMEGVGGGKVQNAADPGIESLSFASIAEDASPVVRLVRSTLYDA
HHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LKAGASDIHLETDARGLTVKYRIDGVLSHVAQIAGTDVAEQAVSRIKVMSELDIAERRVP
HHCCCCCEEEEECCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
QDGRFKVAMQGREVDVRVSVMPSVFGEDAVLRILDRQALSEELAGLTLEGLGYAGTSLAR
CCCCEEEEEECCEEEEEEEECCHHHCCHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
IRKLAAEPYGMLLVTGPTGSGKTTTLYAAISEINHGRDKIVTIEDPVEYQLPGVLQIPVN
HHHHHCCCCCEEEEECCCCCCCCEEHHHHHHHHCCCCCEEEEEECCCCCCCCCEEEECCC
EKKGLSFARGLRSILRHDPDKIMVGEIRDADTAQIAVQSALTGHLVFTTVHANNVFDVIG
CCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCEEEEEEEECCCHHHHHH
RFIHMGVDPYSFVSALNGIVAQRLVRMTCQHCAQAATPNAELLELSGLSVDDTRDYNFRQ
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCC
GAGCGHCRGSGYKGRRAVAEILLLTDTLRELVVARAPIGQIKEAATRGGTRNLRDEALAL
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHH
VKAGDTTLEEINRVTLVG
HHCCCHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]