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The map label for this gene is epsE [H]

Identifier: 74317313

GI number: 74317313

Start: 1366935

End: 1368635

Strand: Direct

Name: epsE [H]

Synonym: Tbd_1295

Alternate gene names: 74317313

Gene position: 1366935-1368635 (Clockwise)

Preceding gene: 74317312

Following gene: 74317314

Centisome position: 46.98

GC content: 67.9

Gene sequence:

>1701_bases
ATGAGCATCGCGCAACAGGTTGCGGCCGACACCGCGTTTCCGATCAGTGCCGGGCAGTTCGGCGCGGCGCGTGCCGAGGC
GGCGCGCAGCGGGCGCCCGCTGCTGAACGTGCTCGACGAACAGCTCGGCCTGCCGGCGCAGGACTTCGTGCGCCGCCTCG
CGCATACCGTGCATTACCCCGTGCTCGACGCGCACGCGCTGCGTTCGCTCACGCCGGCCTTCGATCTCGTGCCCTACGCC
GAGGCGTTCGCGCACGACTGTCTGGTGCTGCGCAACGCGGGCGGGCAGCTTTACCTGGTTCTGGCCGATCCGTTCGCGCC
GGACCTCGTTGCCTGGGCCGAAGGCCGGCTCGACCGGCCCTTTACGCCGGCGCTCGTCGCGGCGGCCGATCTCGCGGCGC
TGCTCGCGCATCACGAAGACCAGCTGCGCGCGATGGACGCGATGGTGCTGTCCGAGACTGCGCCGGACCGCTCGGCGGAG
GAGATCGAGGACCTCTCGCTCAAGGCGATCAGCGAGGACAGCAGCCCGGTCGTCAAGCTCGTGCGCTCGACGCTCTACGA
CGCGCTCAAGAGCGGCGCGAGCGACGTCCACCTGGAAACCGGCGCGACCGGGCTCGCGATCAAATACCGGATCGACGGCG
TGCTCAGCGCGGTCGGCAACGCAGCCGGGCAGGACACGGCTGAGCAGGTCATTTCGCGCATCAAGGTGCTTGCCGAGCTC
GACATCTCCGAGCGGCGCGTGCCGCAGGACGGCCGCTTCAAGGTCGCGGTGCGCGGACGCGAAGTCGATTTCCGCGTCTC
GATCATGCCGAGCGTGTTCGGCGAGGACGCCGTCCTGCGCGTGCTCGACAAGCAGACGCTCTCCGACCAGATGCAGGGCC
TGCGGCTCGACAGCCTCGGCTTCGACGCCGACAGCATGGCGATGCTGCGGCGCATGGCGCGCGAACCCTACGGAATGATC
CTGGTCACCGGCCCGACCGGTTCTGGCAAGACGACGACGCTTTACGCCGCGCTGTCGGAAGTGAATACCGGCCTCGACAA
GATCATCACGATCGAGGACCCCGTCGAATACCACCTGCCCGGGGTGCTGCAGATCCCGGTCAACGAGAAGAAGGGCCTCA
CTTTCGCGCGCGGCCTGCGCTCGGTGCTGCGGCACGACCCCGACAAGATCATGGTCGGCGAAATCCGCGATCCAGAGACC
GCGCAGATCGCGGTTCAGTCCGCGCTGACCGGCCACCTCGTGTTCACGACCGTGCACGCCAACAACGTCTTCGACGTGCT
CGGCCGCTTCGTCCACATGGGCGTCGACGTATACAGCTTCGTCGCGGCGCTGAACGGCATCTCGGCGCAGCGCCTCGTGC
GCCAGATCTGCAGCCACTGCGCCGAGCTATATGTGCCGAGCGCCGAACTGCTCGCCGAGTCGCGGCTCGCCGCCGACGCC
GTCGCCGGGTTCGACTTCCGCATCGGCCGCGGTTGCGAACACTGCCGCGGCTCGGGTTACCGCGGGCGGATCGCGATCGC
CGAAATTCTCCTGCTCAACGACGAGCTGCGCGAACTCATTCTCGAGCGCGCACCGATCCGGCAACTGAAGGAGGCCGCAC
GCCGCAACGGCGTGCGCTTCCTGCGCGATGCCGCGCTCGAACTCGTGCGCAAAGGGCAGAGCACCTTACAGGAAATCAAT
CGTGTCACTTTTGTCGCCTGA

Upstream 100 bases:

>100_bases
AGCTGTTCGAGCCCTTGCTGATGGCCGTGATCGGGGTCGTCATCGGCGCACTCGTGGTCCTGATGTACATGCCGATCTTC
GAACTCGCGGGGAGCATTCA

Downstream 100 bases:

>100_bases
CGCGCTCTACGTCGCCCTCGCGCCCGAGCACGTCGCCCTCCTGCACGTGCGCGGCGGCCTGCGCCCGCGCGTGCTCGCGC
GCGAGTCGGTCGCCGTGGAT

Product: type II secretion system protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 566; Mature: 565

Protein sequence:

>566_residues
MSIAQQVAADTAFPISAGQFGAARAEAARSGRPLLNVLDEQLGLPAQDFVRRLAHTVHYPVLDAHALRSLTPAFDLVPYA
EAFAHDCLVLRNAGGQLYLVLADPFAPDLVAWAEGRLDRPFTPALVAAADLAALLAHHEDQLRAMDAMVLSETAPDRSAE
EIEDLSLKAISEDSSPVVKLVRSTLYDALKSGASDVHLETGATGLAIKYRIDGVLSAVGNAAGQDTAEQVISRIKVLAEL
DISERRVPQDGRFKVAVRGREVDFRVSIMPSVFGEDAVLRVLDKQTLSDQMQGLRLDSLGFDADSMAMLRRMAREPYGMI
LVTGPTGSGKTTTLYAALSEVNTGLDKIITIEDPVEYHLPGVLQIPVNEKKGLTFARGLRSVLRHDPDKIMVGEIRDPET
AQIAVQSALTGHLVFTTVHANNVFDVLGRFVHMGVDVYSFVAALNGISAQRLVRQICSHCAELYVPSAELLAESRLAADA
VAGFDFRIGRGCEHCRGSGYRGRIAIAEILLLNDELRELILERAPIRQLKEAARRNGVRFLRDAALELVRKGQSTLQEIN
RVTFVA

Sequences:

>Translated_566_residues
MSIAQQVAADTAFPISAGQFGAARAEAARSGRPLLNVLDEQLGLPAQDFVRRLAHTVHYPVLDAHALRSLTPAFDLVPYA
EAFAHDCLVLRNAGGQLYLVLADPFAPDLVAWAEGRLDRPFTPALVAAADLAALLAHHEDQLRAMDAMVLSETAPDRSAE
EIEDLSLKAISEDSSPVVKLVRSTLYDALKSGASDVHLETGATGLAIKYRIDGVLSAVGNAAGQDTAEQVISRIKVLAEL
DISERRVPQDGRFKVAVRGREVDFRVSIMPSVFGEDAVLRVLDKQTLSDQMQGLRLDSLGFDADSMAMLRRMAREPYGMI
LVTGPTGSGKTTTLYAALSEVNTGLDKIITIEDPVEYHLPGVLQIPVNEKKGLTFARGLRSVLRHDPDKIMVGEIRDPET
AQIAVQSALTGHLVFTTVHANNVFDVLGRFVHMGVDVYSFVAALNGISAQRLVRQICSHCAELYVPSAELLAESRLAADA
VAGFDFRIGRGCEHCRGSGYRGRIAIAEILLLNDELRELILERAPIRQLKEAARRNGVRFLRDAALELVRKGQSTLQEIN
RVTFVA
>Mature_565_residues
SIAQQVAADTAFPISAGQFGAARAEAARSGRPLLNVLDEQLGLPAQDFVRRLAHTVHYPVLDAHALRSLTPAFDLVPYAE
AFAHDCLVLRNAGGQLYLVLADPFAPDLVAWAEGRLDRPFTPALVAAADLAALLAHHEDQLRAMDAMVLSETAPDRSAEE
IEDLSLKAISEDSSPVVKLVRSTLYDALKSGASDVHLETGATGLAIKYRIDGVLSAVGNAAGQDTAEQVISRIKVLAELD
ISERRVPQDGRFKVAVRGREVDFRVSIMPSVFGEDAVLRVLDKQTLSDQMQGLRLDSLGFDADSMAMLRRMAREPYGMIL
VTGPTGSGKTTTLYAALSEVNTGLDKIITIEDPVEYHLPGVLQIPVNEKKGLTFARGLRSVLRHDPDKIMVGEIRDPETA
QIAVQSALTGHLVFTTVHANNVFDVLGRFVHMGVDVYSFVAALNGISAQRLVRQICSHCAELYVPSAELLAESRLAADAV
AGFDFRIGRGCEHCRGSGYRGRIAIAEILLLNDELRELILERAPIRQLKEAARRNGVRFLRDAALELVRKGQSTLQEINR
VTFVA

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=429, Percent_Identity=43.3566433566434, Blast_Score=331, Evalue=6e-92,
Organism=Escherichia coli, GI1786296, Length=401, Percent_Identity=38.9027431421446, Blast_Score=272, Evalue=4e-74,
Organism=Escherichia coli, GI87082188, Length=252, Percent_Identity=34.1269841269841, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 61533; Mature: 61402

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAQQVAADTAFPISAGQFGAARAEAARSGRPLLNVLDEQLGLPAQDFVRRLAHTVHYP
CCHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCC
VLDAHALRSLTPAFDLVPYAEAFAHDCLVLRNAGGQLYLVLADPFAPDLVAWAEGRLDRP
CHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCHHHHHHCCCCCCC
FTPALVAAADLAALLAHHEDQLRAMDAMVLSETAPDRSAEEIEDLSLKAISEDSSPVVKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
VRSTLYDALKSGASDVHLETGATGLAIKYRIDGVLSAVGNAAGQDTAEQVISRIKVLAEL
HHHHHHHHHHCCCCCEEEECCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
DISERRVPQDGRFKVAVRGREVDFRVSIMPSVFGEDAVLRVLDKQTLSDQMQGLRLDSLG
CHHHCCCCCCCCEEEEEECCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHCCCEECCCC
FDADSMAMLRRMAREPYGMILVTGPTGSGKTTTLYAALSEVNTGLDKIITIEDPVEYHLP
CCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHEEEECCCHHHCCC
GVLQIPVNEKKGLTFARGLRSVLRHDPDKIMVGEIRDPETAQIAVQSALTGHLVFTTVHA
CEEEECCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCEEEEEEEEC
NNVFDVLGRFVHMGVDVYSFVAALNGISAQRLVRQICSHCAELYVPSAELLAESRLAADA
CHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VAGFDFRIGRGCEHCRGSGYRGRIAIAEILLLNDELRELILERAPIRQLKEAARRNGVRF
HCCCCEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHH
LRDAALELVRKGQSTLQEINRVTFVA
HHHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIAQQVAADTAFPISAGQFGAARAEAARSGRPLLNVLDEQLGLPAQDFVRRLAHTVHYP
CHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCC
VLDAHALRSLTPAFDLVPYAEAFAHDCLVLRNAGGQLYLVLADPFAPDLVAWAEGRLDRP
CHHHHHHHHCCCHHHHHHHHHHHHHHHHEEECCCCEEEEEEECCCCCHHHHHHCCCCCCC
FTPALVAAADLAALLAHHEDQLRAMDAMVLSETAPDRSAEEIEDLSLKAISEDSSPVVKL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHH
VRSTLYDALKSGASDVHLETGATGLAIKYRIDGVLSAVGNAAGQDTAEQVISRIKVLAEL
HHHHHHHHHHCCCCCEEEECCCCCEEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHC
DISERRVPQDGRFKVAVRGREVDFRVSIMPSVFGEDAVLRVLDKQTLSDQMQGLRLDSLG
CHHHCCCCCCCCEEEEEECCCEEEEEEECCHHHCCHHHHHHHHHHHHHHHHCCCEECCCC
FDADSMAMLRRMAREPYGMILVTGPTGSGKTTTLYAALSEVNTGLDKIITIEDPVEYHLP
CCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCHHEEEECCCHHHCCC
GVLQIPVNEKKGLTFARGLRSVLRHDPDKIMVGEIRDPETAQIAVQSALTGHLVFTTVHA
CEEEECCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCEEEEEEEEC
NNVFDVLGRFVHMGVDVYSFVAALNGISAQRLVRQICSHCAELYVPSAELLAESRLAADA
CHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
VAGFDFRIGRGCEHCRGSGYRGRIAIAEILLLNDELRELILERAPIRQLKEAARRNGVRF
HCCCCEECCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHH
LRDAALELVRKGQSTLQEINRVTFVA
HHHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]