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The map label for this gene is serS

Identifier: 74317247

GI number: 74317247

Start: 1287218

End: 1288501

Strand: Direct

Name: serS

Synonym: Tbd_1229

Alternate gene names: 74317247

Gene position: 1287218-1288501 (Clockwise)

Preceding gene: 74317246

Following gene: 74317250

Centisome position: 44.24

GC content: 66.43

Gene sequence:

>1284_bases
ATGCTTGATATCCAACTGCTGCGCAAAGACGCGGCCCTCGTCGCCGAGCGCCTCGCGGCGCGGGGCTTTGCCTTCGATGC
CGCGCGCTTCGATGCGCTCGAAGCCGAGCGCAAGACGATCCAGACCCGTACCCAGGATGCCCAGAGCCGTCGCAACACGC
TCTCGAAACAGATCGGCATGTTGAAAGGCAAGGGCGAGGACACGACGGCGGTGATGGCCGAGGTCGGCGGGCTCGGGGAC
GAGCTGAAGCAGCTGGAGACCCGGCTTTCCGAACTGCAGGCCGAGCTCAACGATTTCCTGATGGGCATTCCGAACCTGCC
GCACGAATCCGTTGCGCCGGGAAAGGACGAAACCGCCAATGTCGAAGTCAGCCGCTGGGGGACACCGCGTCAGTTCGATT
TCGCGGTGCGTGACCACGTCGACCTCGGCGAGGGGCTCGGCCAGCTCGACTTCGCCGCCGCAGTCAAGATCACCGGCAGC
CGCTTCAGCGTGATGCGGGGCGGCCTGGCGCGGCTGCACCGCGCGCTCGCGCAGTTCATGCTCGACCTGCACACGACCGA
GCACGGCTACACCGAGGTCTACGTCCCTTACGTCGTCAACGCCGAGTCGATGCGCGGCACCGGCCAGCTGCCGAAGTTCG
AGGAGGACTTGTTCCACGTTCCGCGCAGCGATGCCGACAGGCTCTACCTGATCCCGACGGCCGAAGTCCCCGTCACCAAC
CTCGTGCGCGACGAGATCGTTGCGGCCGAAGCGCTGCCGCTGAAATTCGTCGCCCACACCCCGTGCTTCCGCTCGGAGGC
CGGCTCCTACGGCCGCGACACGCGCGGTATGATCCGCCAGCACCAGTTCGACAAGGTCGAGCTCGTGCAGATGGTCAGGC
CCGAGGACTCGTACGCCGCGCTGGAAGGACTGGCTGCGCACGCCGAGACGGTCCTGCAGCGGCTCGGCCTGCCTTACCGC
AAGATGGCGCTGTGCAGCGGCGACATGGGCTTCTCGGCGGCCAAGACCTACGACCTCGAAGTCTGGCTGCCGGCGCAGAA
TACCTATCGCGAAATTTCGTCGTGCTCGAACTTCGAAGCCTTCCAGGCGCGGCGCATGCAGGCGCGGTTCCGCGAGGGGC
AGGGCAAACCCGAACTTCTGCATACCTTGAACGGGTCGGGGCTCGCGGTCGGGCGCACGCTCGTCGCGATCCTCGAAAAC
TATCAGAACGCCGACGGCAGCATCACGGTCCCCGAGGCCTTGCGTCCGTGGATGGGCGGTGTCGAGCGGCTGACGCCGGC
CTGA

Upstream 100 bases:

>100_bases
GCGGTCGGCATGGTCGAAGCGGCCTGATTCGGTTCCGCCCCTAGCGGGGAACCCGCGCGATCGGCGAAAATGGCGCCTTT
CGCGCACCGATCCCGAGACC

Downstream 100 bases:

>100_bases
GGTCTCAGCCCGGCGGTGGCAGGCCGTCTTCGCCTCGCCCGAGATTGATCAAGGGATCGTATTCGGGCTCGAGCCGCAGC
AGGATGTCGTAGTAGTTGCG

Product: seryl-tRNA synthetase

Products: NA

Alternate protein names: Serine--tRNA ligase; SerRS; Seryl-tRNA(Ser/Sec) synthetase

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MLDIQLLRKDAALVAERLAARGFAFDAARFDALEAERKTIQTRTQDAQSRRNTLSKQIGMLKGKGEDTTAVMAEVGGLGD
ELKQLETRLSELQAELNDFLMGIPNLPHESVAPGKDETANVEVSRWGTPRQFDFAVRDHVDLGEGLGQLDFAAAVKITGS
RFSVMRGGLARLHRALAQFMLDLHTTEHGYTEVYVPYVVNAESMRGTGQLPKFEEDLFHVPRSDADRLYLIPTAEVPVTN
LVRDEIVAAEALPLKFVAHTPCFRSEAGSYGRDTRGMIRQHQFDKVELVQMVRPEDSYAALEGLAAHAETVLQRLGLPYR
KMALCSGDMGFSAAKTYDLEVWLPAQNTYREISSCSNFEAFQARRMQARFREGQGKPELLHTLNGSGLAVGRTLVAILEN
YQNADGSITVPEALRPWMGGVERLTPA

Sequences:

>Translated_427_residues
MLDIQLLRKDAALVAERLAARGFAFDAARFDALEAERKTIQTRTQDAQSRRNTLSKQIGMLKGKGEDTTAVMAEVGGLGD
ELKQLETRLSELQAELNDFLMGIPNLPHESVAPGKDETANVEVSRWGTPRQFDFAVRDHVDLGEGLGQLDFAAAVKITGS
RFSVMRGGLARLHRALAQFMLDLHTTEHGYTEVYVPYVVNAESMRGTGQLPKFEEDLFHVPRSDADRLYLIPTAEVPVTN
LVRDEIVAAEALPLKFVAHTPCFRSEAGSYGRDTRGMIRQHQFDKVELVQMVRPEDSYAALEGLAAHAETVLQRLGLPYR
KMALCSGDMGFSAAKTYDLEVWLPAQNTYREISSCSNFEAFQARRMQARFREGQGKPELLHTLNGSGLAVGRTLVAILEN
YQNADGSITVPEALRPWMGGVERLTPA
>Mature_427_residues
MLDIQLLRKDAALVAERLAARGFAFDAARFDALEAERKTIQTRTQDAQSRRNTLSKQIGMLKGKGEDTTAVMAEVGGLGD
ELKQLETRLSELQAELNDFLMGIPNLPHESVAPGKDETANVEVSRWGTPRQFDFAVRDHVDLGEGLGQLDFAAAVKITGS
RFSVMRGGLARLHRALAQFMLDLHTTEHGYTEVYVPYVVNAESMRGTGQLPKFEEDLFHVPRSDADRLYLIPTAEVPVTN
LVRDEIVAAEALPLKFVAHTPCFRSEAGSYGRDTRGMIRQHQFDKVELVQMVRPEDSYAALEGLAAHAETVLQRLGLPYR
KMALCSGDMGFSAAKTYDLEVWLPAQNTYREISSCSNFEAFQARRMQARFREGQGKPELLHTLNGSGLAVGRTLVAILEN
YQNADGSITVPEALRPWMGGVERLTPA

Specific function: Catalyzes the attachment of serine to tRNA(Ser). Is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec)

COG id: COG0172

COG function: function code J; Seryl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Type-1 seryl-tRNA synthetase subfamily

Homologues:

Organism=Homo sapiens, GI16306548, Length=477, Percent_Identity=34.5911949685535, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI8923421, Length=348, Percent_Identity=30.4597701149425, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI225579074, Length=342, Percent_Identity=30.9941520467836, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI1787120, Length=429, Percent_Identity=64.3356643356643, Blast_Score=565, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17539260, Length=336, Percent_Identity=34.8214285714286, Blast_Score=207, Evalue=7e-54,
Organism=Caenorhabditis elegans, GI17542084, Length=333, Percent_Identity=29.7297297297297, Blast_Score=155, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6320226, Length=452, Percent_Identity=31.1946902654867, Blast_Score=212, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6321799, Length=392, Percent_Identity=30.8673469387755, Blast_Score=190, Evalue=4e-49,
Organism=Drosophila melanogaster, GI24581334, Length=469, Percent_Identity=32.409381663113, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI17737985, Length=341, Percent_Identity=31.6715542521994, Blast_Score=148, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYS_THIDA (Q3SJH9)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314987.1
- HSSP:   Q9N0F3
- ProteinModelPortal:   Q3SJH9
- SMR:   Q3SJH9
- STRING:   Q3SJH9
- GeneID:   3673318
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_1229
- NMPDR:   fig|292415.3.peg.1249
- eggNOG:   COG0172
- HOGENOM:   HBG629391
- OMA:   AHWDLGP
- PhylomeDB:   Q3SJH9
- BioCyc:   TDEN292415:TBD_1229-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00176
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR002317
- InterPro:   IPR015866
- InterPro:   IPR010978
- Gene3D:   G3DSA:1.10.287.40
- PANTHER:   PTHR11778
- PIRSF:   PIRSF001529
- PRINTS:   PR00981
- TIGRFAMs:   TIGR00414

Pfam domain/function: PF02403 Seryl_tRNA_N; PF00587 tRNA-synt_2b; SSF46589 tRNA_binding_arm

EC number: =6.1.1.11

Molecular weight: Translated: 47308; Mature: 47308

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: BINDING 287-287 BINDING 386-386

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIQLLRKDAALVAERLAARGFAFDAARFDALEAERKTIQTRTQDAQSRRNTLSKQIGM
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGKGEDTTAVMAEVGGLGDELKQLETRLSELQAELNDFLMGIPNLPHESVAPGKDETAN
HCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
VEVSRWGTPRQFDFAVRDHVDLGEGLGQLDFAAAVKITGSRFSVMRGGLARLHRALAQFM
EEEECCCCCCEECHHHHHCCCHHCCCCCHHHHEEEEECCCHHHHHHCCHHHHHHHHHHHH
LDLHTTEHGYTEVYVPYVVNAESMRGTGQLPKFEEDLFHVPRSDADRLYLIPTAEVPVTN
HHHHCCCCCCEEEEEEEEEECHHCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCHHH
LVRDEIVAAEALPLKFVAHTPCFRSEAGSYGRDTRGMIRQHQFDKVELVQMVRPEDSYAA
HHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHH
LEGLAAHAETVLQRLGLPYRKMALCSGDMGFSAAKTYDLEVWLPAQNTYREISSCSNFEA
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHCCCCHHH
FQARRMQARFREGQGKPELLHTLNGSGLAVGRTLVAILENYQNADGSITVPEALRPWMGG
HHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHCC
VERLTPA
HHHCCCC
>Mature Secondary Structure
MLDIQLLRKDAALVAERLAARGFAFDAARFDALEAERKTIQTRTQDAQSRRNTLSKQIGM
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKGKGEDTTAVMAEVGGLGDELKQLETRLSELQAELNDFLMGIPNLPHESVAPGKDETAN
HCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
VEVSRWGTPRQFDFAVRDHVDLGEGLGQLDFAAAVKITGSRFSVMRGGLARLHRALAQFM
EEEECCCCCCEECHHHHHCCCHHCCCCCHHHHEEEEECCCHHHHHHCCHHHHHHHHHHHH
LDLHTTEHGYTEVYVPYVVNAESMRGTGQLPKFEEDLFHVPRSDADRLYLIPTAEVPVTN
HHHHCCCCCCEEEEEEEEEECHHCCCCCCCCCHHHHHHCCCCCCCCEEEEEECCCCCHHH
LVRDEIVAAEALPLKFVAHTPCFRSEAGSYGRDTRGMIRQHQFDKVELVQMVRPEDSYAA
HHHHHHHHHHCCCCHHHHCCCCHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHCCCCHHHH
LEGLAAHAETVLQRLGLPYRKMALCSGDMGFSAAKTYDLEVWLPAQNTYREISSCSNFEA
HHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEEECCCHHHHHHHCCCCHHH
FQARRMQARFREGQGKPELLHTLNGSGLAVGRTLVAILENYQNADGSITVPEALRPWMGG
HHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCEECHHHHHHHHCC
VERLTPA
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA