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The map label for this gene is trmJ [H]

Identifier: 74317179

GI number: 74317179

Start: 1215819

End: 1216589

Strand: Direct

Name: trmJ [H]

Synonym: Tbd_1161

Alternate gene names: 74317179

Gene position: 1215819-1216589 (Clockwise)

Preceding gene: 74317175

Following gene: 74317180

Centisome position: 41.78

GC content: 68.09

Gene sequence:

>771_bases
ATGACATCGAGCGATCCTAAACTTCTCGCCGACATTCGCGTGGTGCTCGCGCGCACGAGTCACCCAGGCAACATCGGCGC
CGCGGCACGCGCGATGAAGACGATGGGATTGTCGCAACTGGTTCTGGTTGATCCCGCGGTATTTCCCAACAGCCAGGCCG
ACGCGATGGCCTCGGGCGCCGTCGACGTGCTTGCCGGCGCACGCGTTTGCCCGACCCTGACCGAAGCCCTCGCCGACACC
ACGCTCGCGCTCGGCGTCTCGGCGCGCCGACGCGACATCGTCGCCGAGGTGCTGAATCCCGCGGAGGCAGCGCTTCGGCT
GCTCGGCGAGGCGCAGCGCGCGCCCGTCGCGCTGGTGTTCGGCAACGAGACCAGCGGACTGTCGAACGAGGAGCTGAGTC
TGTGCCAAGGGCTCGTGACGATCCCGGCCAACCCCGACTACAGTTCCCTCAACCTCGCCGCAGCGGTCCAGGTGCTGAGC
TACGAGATCCGCCAGGCCTGGCTCGACCGTCCGTCCTGGCCGGCGCCCGAGATCGACGCCGCGAGCGGAGACGAAGTCGA
GAAGTTCTACGGCCATCTCGAGGCCGCGCTCGCCGACCTCGAATTCCTCAATCCCGGCTCGCCGGGCAAGCTCATGCTCA
AGCTCCGGCGCCTGTTCGCACGCACCCGCCTGGCGAAGGAGGAAGTCAACATCCTGCGCGGCATCCTCAGCGCGGCGCAG
GACGCCAAGCGGGCCGCGCGCCGCAGCGGAACCGTCGGGCATAATAGTTGA

Upstream 100 bases:

>100_bases
TGATCGCCCCCGCCTTGCGTGCGGCTTTCACCGCCGTATTGAGCATGGGATGCATGATGTCTGGCAATGGATTCAAAGAA
CGAGCGCGCCATTTTACTTG

Downstream 100 bases:

>100_bases
CTAAAATACTCGGAAATAGCATCCTCATCGCATGAGCCTGTTCAGCCAACTGAAGGAAGACATCGCGGTCGTCTTCGACC
GCGATCCCGCGGCGCGCTCG

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTSSDPKLLADIRVVLARTSHPGNIGAAARAMKTMGLSQLVLVDPAVFPNSQADAMASGAVDVLAGARVCPTLTEALADT
TLALGVSARRRDIVAEVLNPAEAALRLLGEAQRAPVALVFGNETSGLSNEELSLCQGLVTIPANPDYSSLNLAAAVQVLS
YEIRQAWLDRPSWPAPEIDAASGDEVEKFYGHLEAALADLEFLNPGSPGKLMLKLRRLFARTRLAKEEVNILRGILSAAQ
DAKRAARRSGTVGHNS

Sequences:

>Translated_256_residues
MTSSDPKLLADIRVVLARTSHPGNIGAAARAMKTMGLSQLVLVDPAVFPNSQADAMASGAVDVLAGARVCPTLTEALADT
TLALGVSARRRDIVAEVLNPAEAALRLLGEAQRAPVALVFGNETSGLSNEELSLCQGLVTIPANPDYSSLNLAAAVQVLS
YEIRQAWLDRPSWPAPEIDAASGDEVEKFYGHLEAALADLEFLNPGSPGKLMLKLRRLFARTRLAKEEVNILRGILSAAQ
DAKRAARRSGTVGHNS
>Mature_255_residues
TSSDPKLLADIRVVLARTSHPGNIGAAARAMKTMGLSQLVLVDPAVFPNSQADAMASGAVDVLAGARVCPTLTEALADTT
LALGVSARRRDIVAEVLNPAEAALRLLGEAQRAPVALVFGNETSGLSNEELSLCQGLVTIPANPDYSSLNLAAAVQVLSY
EIRQAWLDRPSWPAPEIDAASGDEVEKFYGHLEAALADLEFLNPGSPGKLMLKLRRLFARTRLAKEEVNILRGILSAAQD
AKRAARRSGTVGHNS

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=249, Percent_Identity=48.995983935743, Blast_Score=231, Evalue=3e-62,
Organism=Escherichia coli, GI1790865, Length=154, Percent_Identity=40.9090909090909, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27056; Mature: 26925

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSDPKLLADIRVVLARTSHPGNIGAAARAMKTMGLSQLVLVDPAVFPNSQADAMASGA
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHEEEECCCCCCCCCHHHHHHCH
VDVLAGARVCPTLTEALADTTLALGVSARRRDIVAEVLNPAEAALRLLGEAQRAPVALVF
HHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEE
GNETSGLSNEELSLCQGLVTIPANPDYSSLNLAAAVQVLSYEIRQAWLDRPSWPAPEIDA
CCCCCCCCHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ASGDEVEKFYGHLEAALADLEFLNPGSPGKLMLKLRRLFARTRLAKEEVNILRGILSAAQ
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAKRAARRSGTVGHNS
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TSSDPKLLADIRVVLARTSHPGNIGAAARAMKTMGLSQLVLVDPAVFPNSQADAMASGA
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHEEEECCCCCCCCCHHHHHHCH
VDVLAGARVCPTLTEALADTTLALGVSARRRDIVAEVLNPAEAALRLLGEAQRAPVALVF
HHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEEEEE
GNETSGLSNEELSLCQGLVTIPANPDYSSLNLAAAVQVLSYEIRQAWLDRPSWPAPEIDA
CCCCCCCCHHHHHHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
ASGDEVEKFYGHLEAALADLEFLNPGSPGKLMLKLRRLFARTRLAKEEVNILRGILSAAQ
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DAKRAARRSGTVGHNS
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA