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The map label for this gene is nuoF [H]

Identifier: 74317165

GI number: 74317165

Start: 1200298

End: 1201578

Strand: Direct

Name: nuoF [H]

Synonym: Tbd_1147

Alternate gene names: 74317165

Gene position: 1200298-1201578 (Clockwise)

Preceding gene: 74317164

Following gene: 74317166

Centisome position: 41.25

GC content: 64.25

Gene sequence:

>1281_bases
ATGAGTCTGCTCACGCCCACGCCGCAGGTCTGCAGCTGGACGCAGACGCTCGACGATCCGGCATCGCTCGCCACCTACGT
CGCCAACGGCGGGTACCAGGCTCTGCGCCGCGTCGTCACCGAAGCGGTCCCGGGTGACGACATCATCGCCGAGATCAAGA
CCAGCGCGTTGCGCGGGCGGGGCGGGGCGGGGTTTCCGACCGGCCTCAAGTGGAGCTTCATGCCGCGTGCGTTTCCGGGC
GACAAGTACATCGTCTGCAATAGCGACGAAGGCGAGCCGGGCACCTTCAAGGACCGCGATATCCTGCGCTACAACCCGCA
CCAGCTGATCGAAGGCATGGCGATCGCCGGGTACGTGCTCGGCGCGCGCGTGGGTTACAACTATATCCACGGAGAGATTT
TCGAGGTCTACCAGATCTTCGAACGCGCGCTCGAGGAAGCACGGCAAGCGGGCTACCTCGGCGCCAACCTGTTCGGAACC
GATTTCTGCTTCGACCTGCACGCCCACCACGGCTACGGTGCGTACATCTGCGGCGAAGAAACGGCCCTGCTCGAGTCGAT
CGAAGGCAAGAAGGGGCAGCCGCGCTTCAAGCCGCCGTTTCCCGCAAGCTTCGGTCTCTACGGCAAGCCGACCACGATCA
ACAATACCGAAACGCTGGCGAGCGTCCCCTGGATCGTGCAGCACGGCGGCCAGGCCTTCCTCGAGCTCGGCAAGCCGAAC
AACGGCGGCGCCAAGCTGTTTTCGGTATCCGGCCATGTCAACCGGCCGGGCAATTACGAGGTGCCGCTCGGCACGCCGTT
CACCGAATTGCTGGAAATGGCCGGCGGCGTGCGCGACGGCCGCAGCCTGAAGGCCGTCATCCCCGGCGGATCTTCGGCGC
CGGTGTTGCCGGGGCACATCATGATGGAGACGACGATGGATTTCGATTCCATCGCCAAGGCCGGCTCGATGCTCGGCTCC
GGGGCCGTCATCGTGATGGACGAAACCGTATGCATGGTGCGCGCGCTCGAGCGGCTCTCGTACTTCTATTACGAGGAGTC
GTGCGGCCAGTGCACGCCCTGCCGCGAGGGGACCGGATGGATGTATCGCGTGATCCATCGCATCGAGAACGGGCAGGGAC
GCCCCGAAGACATGGATCTCCTCAACAGTGTCGCAGGCCAGATCGGCGGACACACGATCTGCGCCCTCGGTGACGCGGCG
GCCATGCCGGTGCAAAGCTTCCTCAAACATTTCGGCCACGAGTTCGAGCATCACATCGAACACAAGCGCTGCATGGTCTG
A

Upstream 100 bases:

>100_bases
TGGGGGCATGCGGCGATGCGCCGATGTGCCTGCACAACAATCACACGATGCACACGCACCTTACGCCAGAGAAGGTCGAC
GAGTTGCTGGACAAGCTGAA

Downstream 100 bases:

>100_bases
CGGTACGACAACGATGGCTACCCTGAATATCGAAATCGATGGGCGCGCGCTGCAGGTCGAAAGCGGCGACACCATCATGG
ACGCCGCGAACCAGGCGGGT

Product: NADH-quinone oxidoreductase subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSLLTPTPQVCSWTQTLDDPASLATYVANGGYQALRRVVTEAVPGDDIIAEIKTSALRGRGGAGFPTGLKWSFMPRAFPG
DKYIVCNSDEGEPGTFKDRDILRYNPHQLIEGMAIAGYVLGARVGYNYIHGEIFEVYQIFERALEEARQAGYLGANLFGT
DFCFDLHAHHGYGAYICGEETALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTETLASVPWIVQHGGQAFLELGKPN
NGGAKLFSVSGHVNRPGNYEVPLGTPFTELLEMAGGVRDGRSLKAVIPGGSSAPVLPGHIMMETTMDFDSIAKAGSMLGS
GAVIVMDETVCMVRALERLSYFYYEESCGQCTPCREGTGWMYRVIHRIENGQGRPEDMDLLNSVAGQIGGHTICALGDAA
AMPVQSFLKHFGHEFEHHIEHKRCMV

Sequences:

>Translated_426_residues
MSLLTPTPQVCSWTQTLDDPASLATYVANGGYQALRRVVTEAVPGDDIIAEIKTSALRGRGGAGFPTGLKWSFMPRAFPG
DKYIVCNSDEGEPGTFKDRDILRYNPHQLIEGMAIAGYVLGARVGYNYIHGEIFEVYQIFERALEEARQAGYLGANLFGT
DFCFDLHAHHGYGAYICGEETALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTETLASVPWIVQHGGQAFLELGKPN
NGGAKLFSVSGHVNRPGNYEVPLGTPFTELLEMAGGVRDGRSLKAVIPGGSSAPVLPGHIMMETTMDFDSIAKAGSMLGS
GAVIVMDETVCMVRALERLSYFYYEESCGQCTPCREGTGWMYRVIHRIENGQGRPEDMDLLNSVAGQIGGHTICALGDAA
AMPVQSFLKHFGHEFEHHIEHKRCMV
>Mature_425_residues
SLLTPTPQVCSWTQTLDDPASLATYVANGGYQALRRVVTEAVPGDDIIAEIKTSALRGRGGAGFPTGLKWSFMPRAFPGD
KYIVCNSDEGEPGTFKDRDILRYNPHQLIEGMAIAGYVLGARVGYNYIHGEIFEVYQIFERALEEARQAGYLGANLFGTD
FCFDLHAHHGYGAYICGEETALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTETLASVPWIVQHGGQAFLELGKPNN
GGAKLFSVSGHVNRPGNYEVPLGTPFTELLEMAGGVRDGRSLKAVIPGGSSAPVLPGHIMMETTMDFDSIAKAGSMLGSG
AVIVMDETVCMVRALERLSYFYYEESCGQCTPCREGTGWMYRVIHRIENGQGRPEDMDLLNSVAGQIGGHTICALGDAAA
MPVQSFLKHFGHEFEHHIEHKRCMV

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI20149568, Length=379, Percent_Identity=53.0343007915567, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI260656005, Length=379, Percent_Identity=53.0343007915567, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1788620, Length=427, Percent_Identity=43.3255269320843, Blast_Score=353, Evalue=9e-99,
Organism=Caenorhabditis elegans, GI17531727, Length=408, Percent_Identity=49.2647058823529, Blast_Score=410, Evalue=1e-115,
Organism=Drosophila melanogaster, GI19920794, Length=403, Percent_Identity=50.1240694789082, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI45550452, Length=404, Percent_Identity=47.2772277227723, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24653819, Length=377, Percent_Identity=38.1962864721485, Blast_Score=256, Evalue=1e-68,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=36.6197183098592, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 46368; Mature: 46237

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLLTPTPQVCSWTQTLDDPASLATYVANGGYQALRRVVTEAVPGDDIIAEIKTSALRGR
CCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
GGAGFPTGLKWSFMPRAFPGDKYIVCNSDEGEPGTFKDRDILRYNPHQLIEGMAIAGYVL
CCCCCCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHH
GARVGYNYIHGEIFEVYQIFERALEEARQAGYLGANLFGTDFCFDLHAHHGYGAYICGEE
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCH
TALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTETLASVPWIVQHGGQAFLELGKPN
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHCCCCEEEEECCCC
NGGAKLFSVSGHVNRPGNYEVPLGTPFTELLEMAGGVRDGRSLKAVIPGGSSAPVLPGHI
CCCEEEEEECCCCCCCCCEECCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCE
MMETTMDFDSIAKAGSMLGSGAVIVMDETVCMVRALERLSYFYYEESCGQCTPCREGTGW
EEEECCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
MYRVIHRIENGQGRPEDMDLLNSVAGQIGGHTICALGDAAAMPVQSFLKHFGHEFEHHIE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCHHHHHHHH
HKRCMV
HHHCCC
>Mature Secondary Structure 
SLLTPTPQVCSWTQTLDDPASLATYVANGGYQALRRVVTEAVPGDDIIAEIKTSALRGR
CCCCCCHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
GGAGFPTGLKWSFMPRAFPGDKYIVCNSDEGEPGTFKDRDILRYNPHQLIEGMAIAGYVL
CCCCCCCCCEECCCCCCCCCCEEEEECCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHH
GARVGYNYIHGEIFEVYQIFERALEEARQAGYLGANLFGTDFCFDLHAHHGYGAYICGEE
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCEEEECCH
TALLESIEGKKGQPRFKPPFPASFGLYGKPTTINNTETLASVPWIVQHGGQAFLELGKPN
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHCCCCEEEEECCCC
NGGAKLFSVSGHVNRPGNYEVPLGTPFTELLEMAGGVRDGRSLKAVIPGGSSAPVLPGHI
CCCEEEEEECCCCCCCCCEECCCCCCHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCE
MMETTMDFDSIAKAGSMLGSGAVIVMDETVCMVRALERLSYFYYEESCGQCTPCREGTGW
EEEECCCHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
MYRVIHRIENGQGRPEDMDLLNSVAGQIGGHTICALGDAAAMPVQSFLKHFGHEFEHHIE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCHHHHHHHH
HKRCMV
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]