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The map label for this gene is btuF [H]

Identifier: 74317140

GI number: 74317140

Start: 1177570

End: 1178433

Strand: Direct

Name: btuF [H]

Synonym: Tbd_1122

Alternate gene names: 74317140

Gene position: 1177570-1178433 (Clockwise)

Preceding gene: 74317139

Following gene: 74317142

Centisome position: 40.47

GC content: 70.72

Gene sequence:

>864_bases
GTGACCCGGCGGGGCATCCTCGCGCTGTGCCTGCTGCTCGCCGGGGTGACGCACGCCGCCCCGGTCCGCGTCGTCGACGA
CGCCGGGCTCACGCTGGAGCTTGCCCGAACGCCGCAACGCATCGTTTCCCTGACGCCGCACCTGACCGAGCTGTTGTTCG
CGGTCGGCGCCGGCGAACAGTTGGTGGGGGTCGACAGCGCGAGCGACTTCCCGGAAGCGGCACGCGGTCTGCCGCGCGTC
GGCGACTACAGCCGCGTCAGCTTCGAGCGCATCCTCGCGCTCAAGCCTGATCTCGTGCTTGTCTGGCTCGGTGGCAATCG
CCCGGTCGACATCCACGGCCTGAAAGCCATGAAGCTGCCGCTCCTGCATACGCGTGCGGCGAAGCTCGACGACGTCGCGC
GCCTGCTGCGCCTGCTCGGACGGGCGAGCGGGCATCCAGTGCAAGGGGAGCGGGCGGCGCAACAGTTCGCGCAGCGGCTT
GCAGTGCTCGAGGAGCGCTACGCAAGCAGGCGGCCGATCCCGGTCTTCTACCAGGTATGGGACCGCCCGTTGATGACGGT
GGGCGGCGGGCACTGGATCAGTGAGGCGCTGACAGTGTGCGGCGCGCGCAACGTCTTCGCCGACCTGGCGGCGCTCTCGC
CGGTGGTGTCGCGCGAGGCGGTGCTGACGCGTGGGCCCGAAGTGATCGTCAGCGGCAGCGACGCGCCGGCCGTCCGATCC
CAGTGGGAGCGCTATGCGGAATTGCCGGCGGCCAGGACGGGCGCCTTTCTGCGGATAGACGCCGACCGGCTGCACCGCCC
GAGCCCGCGTCTGCTCGACGGGGTCGTGCAGTTATGTGAGGCGATCGCCCCTTACGGCCGCTGA

Upstream 100 bases:

>100_bases
AGGCCTACCGGATCGAAGTGGCGAATGCCTCGTTCACGCGTCTGCGTTGCGAGGGCGGCCGATCGACACTGGTTTTTCAC
GGCAGCGCGCGCGTCGAGGC

Downstream 100 bases:

>100_bases
CGGTCGACGGACTTGCGGTTGGGGCAGTTTTCCTTGATGCAATCGGCGTAGATCTGCAGCGAGTGGTCGTGGATCGCGAA
GCCGCGTTCCTTTGCGATGC

Product: substrate-binding periplasmic (PBP) ABC transporter protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MTRRGILALCLLLAGVTHAAPVRVVDDAGLTLELARTPQRIVSLTPHLTELLFAVGAGEQLVGVDSASDFPEAARGLPRV
GDYSRVSFERILALKPDLVLVWLGGNRPVDIHGLKAMKLPLLHTRAAKLDDVARLLRLLGRASGHPVQGERAAQQFAQRL
AVLEERYASRRPIPVFYQVWDRPLMTVGGGHWISEALTVCGARNVFADLAALSPVVSREAVLTRGPEVIVSGSDAPAVRS
QWERYAELPAARTGAFLRIDADRLHRPSPRLLDGVVQLCEAIAPYGR

Sequences:

>Translated_287_residues
MTRRGILALCLLLAGVTHAAPVRVVDDAGLTLELARTPQRIVSLTPHLTELLFAVGAGEQLVGVDSASDFPEAARGLPRV
GDYSRVSFERILALKPDLVLVWLGGNRPVDIHGLKAMKLPLLHTRAAKLDDVARLLRLLGRASGHPVQGERAAQQFAQRL
AVLEERYASRRPIPVFYQVWDRPLMTVGGGHWISEALTVCGARNVFADLAALSPVVSREAVLTRGPEVIVSGSDAPAVRS
QWERYAELPAARTGAFLRIDADRLHRPSPRLLDGVVQLCEAIAPYGR
>Mature_286_residues
TRRGILALCLLLAGVTHAAPVRVVDDAGLTLELARTPQRIVSLTPHLTELLFAVGAGEQLVGVDSASDFPEAARGLPRVG
DYSRVSFERILALKPDLVLVWLGGNRPVDIHGLKAMKLPLLHTRAAKLDDVARLLRLLGRASGHPVQGERAAQQFAQRLA
VLEERYASRRPIPVFYQVWDRPLMTVGGGHWISEALTVCGARNVFADLAALSPVVSREAVLTRGPEVIVSGSDAPAVRSQ
WERYAELPAARTGAFLRIDADRLHRPSPRLLDGVVQLCEAIAPYGR

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=247, Percent_Identity=35.2226720647773, Blast_Score=142, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 31194; Mature: 31062

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRGILALCLLLAGVTHAAPVRVVDDAGLTLELARTPQRIVSLTPHLTELLFAVGAGEQ
CCCHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCHHHHHHCCHHHHHHHHHHCCCCC
LVGVDSASDFPEAARGLPRVGDYSRVSFERILALKPDLVLVWLGGNRPVDIHGLKAMKLP
EEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEECCCHHHHCC
LLHTRAAKLDDVARLLRLLGRASGHPVQGERAAQQFAQRLAVLEERYASRRPIPVFYQVW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
DRPLMTVGGGHWISEALTVCGARNVFADLAALSPVVSREAVLTRGPEVIVSGSDAPAVRS
CCCCEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
QWERYAELPAARTGAFLRIDADRLHRPSPRLLDGVVQLCEAIAPYGR
HHHHHHHCCCHHCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TRRGILALCLLLAGVTHAAPVRVVDDAGLTLELARTPQRIVSLTPHLTELLFAVGAGEQ
CCHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCCHHHHHHCCHHHHHHHHHHCCCCC
LVGVDSASDFPEAARGLPRVGDYSRVSFERILALKPDLVLVWLGGNRPVDIHGLKAMKLP
EEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCEEECCCHHHHCC
LLHTRAAKLDDVARLLRLLGRASGHPVQGERAAQQFAQRLAVLEERYASRRPIPVFYQVW
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
DRPLMTVGGGHWISEALTVCGARNVFADLAALSPVVSREAVLTRGPEVIVSGSDAPAVRS
CCCCEECCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
QWERYAELPAARTGAFLRIDADRLHRPSPRLLDGVVQLCEAIAPYGR
HHHHHHHCCCHHCCCEEEEEHHHCCCCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]