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The map label for this gene is yafC [C]

Identifier: 74317134

GI number: 74317134

Start: 1172671

End: 1173621

Strand: Reverse

Name: yafC [C]

Synonym: Tbd_1116

Alternate gene names: 74317134

Gene position: 1173621-1172671 (Counterclockwise)

Preceding gene: 74317141

Following gene: 74317132

Centisome position: 40.33

GC content: 69.09

Gene sequence:

>951_bases
ATGGACCGCTTAGCGCATATGCAGATGTTCGTCGCCGTCGTCGAAGCCGGCAGCATCACCGCGGCCGCGGAACGCATGAA
CCTCGCCAAGTCGGCGGTCAGCCGGCGTCTTGCTGAACTCGAGGCGGGCCTCGGCGTCTCGCTGATTCAGCGCACGACGC
GTCGCCTCAACCTCACCGAAAGCGGGCGTACCTACTACGCGCGCTGCGTCGCGATCCTCGCCGACGTCGAGGACGCCGAG
GCCGAGGTCTCGCAGGGGCACGGGGCGCTGCGTGGACGCCTCAAGGTCGCGCTACCGCAGGCCTTCGGCCTGCTCCATCT
CGCGCCGCTGATCCAGACCTTCATGAAGCGGCACCCGGACGTCCGCCTCGAACTCGACTTCAACGATCGGCAGGTCGACC
TGATGCAGGAAGGCTTCGACCTCGCCATACGGATCGCCACGCTTGCCGATTCGACCCTGATCGCGCGCCGACTCGCGGCG
ATTCCCCACCGGGTCGTCGCGAGCCCCGGCTACCTGGCGCGACGCGGAACGCCTCGGGCGGCCGCGGATCTGACCCAGCA
TGACTGCCTCGCCTACAGCAACGTGCGCGACCCGGGCCTGTGGAGCTATCGCGATCCGGGCGGCGAGCCCGGCCAGGTGC
GCGTAGTGGTCCGGCTCGCCGCGAGCAGCGGCGAATTCCTGTTGCAGGCGGCGATTGCCGACGAAGGCCTCGTCCTGCTG
CCCGCGTTCTACCTGCACGCCGCGCTGCGATCGGGCCAGTTGAGCGAAGTGCTGGCGGATCATCGATGGCCGGAACCCGC
CGCCTATGCCGTCTATCCGCCGACGCGCCATCTTTCCGCCCGCGCGCACGCTTTCATCGACTTTCTCGCCGGCCATCTCG
GCGGCGCCGGCGAACCGCCGGCTGGCGACGAGGTCGCGCCTACTCTCGCTCCGAATACGTCGGCAGGGTGA

Upstream 100 bases:

>100_bases
AGAAGGGCGTCAAAAAGTGACGCGGTGATGCCATTATTTGTTTTGCCGGATAGAGAATAAAGGCGGTGTTTATGAAATAA
GTGTTGCTAAAATCTGAACA

Downstream 100 bases:

>100_bases
TGCGGAAGGCCATCGCCGCCAGCCGGACGACGAGAACGGTCGCCATCCCCGCCCACGCCGCGGCGACCGGCGACACCGAC
AAGGCCTGCAGCCCGACCAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MDRLAHMQMFVAVVEAGSITAAAERMNLAKSAVSRRLAELEAGLGVSLIQRTTRRLNLTESGRTYYARCVAILADVEDAE
AEVSQGHGALRGRLKVALPQAFGLLHLAPLIQTFMKRHPDVRLELDFNDRQVDLMQEGFDLAIRIATLADSTLIARRLAA
IPHRVVASPGYLARRGTPRAAADLTQHDCLAYSNVRDPGLWSYRDPGGEPGQVRVVVRLAASSGEFLLQAAIADEGLVLL
PAFYLHAALRSGQLSEVLADHRWPEPAAYAVYPPTRHLSARAHAFIDFLAGHLGGAGEPPAGDEVAPTLAPNTSAG

Sequences:

>Translated_316_residues
MDRLAHMQMFVAVVEAGSITAAAERMNLAKSAVSRRLAELEAGLGVSLIQRTTRRLNLTESGRTYYARCVAILADVEDAE
AEVSQGHGALRGRLKVALPQAFGLLHLAPLIQTFMKRHPDVRLELDFNDRQVDLMQEGFDLAIRIATLADSTLIARRLAA
IPHRVVASPGYLARRGTPRAAADLTQHDCLAYSNVRDPGLWSYRDPGGEPGQVRVVVRLAASSGEFLLQAAIADEGLVLL
PAFYLHAALRSGQLSEVLADHRWPEPAAYAVYPPTRHLSARAHAFIDFLAGHLGGAGEPPAGDEVAPTLAPNTSAG
>Mature_316_residues
MDRLAHMQMFVAVVEAGSITAAAERMNLAKSAVSRRLAELEAGLGVSLIQRTTRRLNLTESGRTYYARCVAILADVEDAE
AEVSQGHGALRGRLKVALPQAFGLLHLAPLIQTFMKRHPDVRLELDFNDRQVDLMQEGFDLAIRIATLADSTLIARRLAA
IPHRVVASPGYLARRGTPRAAADLTQHDCLAYSNVRDPGLWSYRDPGGEPGQVRVVVRLAASSGEFLLQAAIADEGLVLL
PAFYLHAALRSGQLSEVLADHRWPEPAAYAVYPPTRHLSARAHAFIDFLAGHLGGAGEPPAGDEVAPTLAPNTSAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=37.6712328767123, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=36.026936026936, Blast_Score=169, Evalue=2e-43,
Organism=Escherichia coli, GI1789440, Length=302, Percent_Identity=30.794701986755, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1789639, Length=298, Percent_Identity=30.2013422818792, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI87081978, Length=287, Percent_Identity=32.7526132404181, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787128, Length=295, Percent_Identity=27.7966101694915, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1787589, Length=294, Percent_Identity=29.5918367346939, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789173, Length=251, Percent_Identity=27.4900398406374, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=123, Percent_Identity=26.8292682926829, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788706, Length=204, Percent_Identity=27.4509803921569, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788481, Length=142, Percent_Identity=31.6901408450704, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI157672245, Length=144, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786713, Length=282, Percent_Identity=26.9503546099291, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1786508, Length=117, Percent_Identity=29.0598290598291, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788297, Length=141, Percent_Identity=30.4964539007092, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=30, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34041; Mature: 34041

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLAHMQMFVAVVEAGSITAAAERMNLAKSAVSRRLAELEAGLGVSLIQRTTRRLNLTE
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
SGRTYYARCVAILADVEDAEAEVSQGHGALRGRLKVALPQAFGLLHLAPLIQTFMKRHPD
CCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEECHHHHHHHHHHHHHHHHHHHCCC
VRLELDFNDRQVDLMQEGFDLAIRIATLADSTLIARRLAAIPHRVVASPGYLARRGTPRA
EEEEECCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHCCCCCHHCCCCCHH
AADLTQHDCLAYSNVRDPGLWSYRDPGGEPGQVRVVVRLAASSGEFLLQAAIADEGLVLL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCHHH
PAFYLHAALRSGQLSEVLADHRWPEPAAYAVYPPTRHLSARAHAFIDFLAGHLGGAGEPP
HHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCC
AGDEVAPTLAPNTSAG
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDRLAHMQMFVAVVEAGSITAAAERMNLAKSAVSRRLAELEAGLGVSLIQRTTRRLNLTE
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
SGRTYYARCVAILADVEDAEAEVSQGHGALRGRLKVALPQAFGLLHLAPLIQTFMKRHPD
CCCHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEECHHHHHHHHHHHHHHHHHHHCCC
VRLELDFNDRQVDLMQEGFDLAIRIATLADSTLIARRLAAIPHRVVASPGYLARRGTPRA
EEEEECCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCHHHHCCCCCHHCCCCCHH
AADLTQHDCLAYSNVRDPGLWSYRDPGGEPGQVRVVVRLAASSGEFLLQAAIADEGLVLL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCHHH
PAFYLHAALRSGQLSEVLADHRWPEPAAYAVYPPTRHLSARAHAFIDFLAGHLGGAGEPP
HHHHHHHHHHCCCHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCC
AGDEVAPTLAPNTSAG
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]