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The map label for this gene is moaA [H]

Identifier: 74317041

GI number: 74317041

Start: 1091225

End: 1092208

Strand: Reverse

Name: moaA [H]

Synonym: Tbd_1023

Alternate gene names: 74317041

Gene position: 1092208-1091225 (Counterclockwise)

Preceding gene: 74317052

Following gene: 74317039

Centisome position: 37.54

GC content: 67.38

Gene sequence:

>984_bases
ATGAACGCGCCCGCCCTCGTCGACCAGTTTGGCCGCCACATCGATTATGTGCGGCTTTCCGTGACCGACCGCTGCGACCT
GCGCTGCAGCTACTGCATGCCCGAGGGCTTCAAGGGTTTCGAGGAGCCCGCCGACTGGCTCGACTTCGACGAGATCGAGC
GTCTGCTCGGCGCATTTGCCCGGCTCGGCGTGCGCCGCGTGCGCCTGACCGGCGGCGAGCCCCTGCTGCGCCGCGACATC
AGCGGACTCGCGCGGCGCATCGCGTCCCTGCCCGGGATCGAGGACCTCTCGCTTTCCACCAACGCGACCCAGCTCGACAG
GCACGCCGCCGCGCTGAAGGCCGCGGGCGTGACGCGTCTGAACGTCAGCCTCGATTCGCTGCAGCAGGCACGGGTCGAGC
GGATCAACGGCCGCGACGTGCTGGCAAAGGTGATGACGGGCCTTGCCGCGGCGCAGGAAGCGGGCTTTGCGCCGATCAAG
CTCAACATGGTCGCGATGCGCGGCAGCAACGACGACGAAATCGACGAGATGGTCGCCTTCTGCATGGACCGCGGCTTCGT
CCTGCGCCTCATCGAGGCGATGCCGATGGGTGCGACCGGCCGCAACACCGAGTACCTGGACCTGCAGCCGGTAAAGGAAC
GCCTGCGCGCACGCTTCGGCCTCGTCGAAACCGCGCTGCCGGGCGGCGGGCCTGCGCGTTATCTCGGCACTGCCGACGGC
GGCTTCAACGTCGGCTTCATCACGCCGATCTCGCAGCATTTCTGCGAAAGCTGCAACCGCATCCGCATCGCGGTCGACGG
CACGGCATACATGTGCCTCGGGCAGGACGAGAAATTCGAGTTCCGCCCGCTTCTGCGCGACGGCTGTTCGGACGCCGAGC
TCGACGCGGCGATCGTCGAGGCAATCAACCTCAAGCCCGAACGCCACGAATTCCGCGAGGCGCCGCACAAGCTCGTGCGC
TTCATGTCGATGACCGGCGGTTAG

Upstream 100 bases:

>100_bases
CGCTGTAGCCGGCGATGCCGAAGACTTTCATGCCGTTGCCATCGGAAAGGAAGAGGGTTATATTTTTTCATATATAACCC
GTCGTTGCCAATCACCAGCT

Downstream 100 bases:

>100_bases
TCGCCTACTCGGCGCTCGCGGTGCGAAACCCGGCGTAAGGATCGCGGCGGTGCGGCAGATAGAAATTGCGAAACCCGGGT
CGCTTGAGCGCGGCGTCGGT

Product: GTP cyclohydrolase subunit MoaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNAPALVDQFGRHIDYVRLSVTDRCDLRCSYCMPEGFKGFEEPADWLDFDEIERLLGAFARLGVRRVRLTGGEPLLRRDI
SGLARRIASLPGIEDLSLSTNATQLDRHAAALKAAGVTRLNVSLDSLQQARVERINGRDVLAKVMTGLAAAQEAGFAPIK
LNMVAMRGSNDDEIDEMVAFCMDRGFVLRLIEAMPMGATGRNTEYLDLQPVKERLRARFGLVETALPGGGPARYLGTADG
GFNVGFITPISQHFCESCNRIRIAVDGTAYMCLGQDEKFEFRPLLRDGCSDAELDAAIVEAINLKPERHEFREAPHKLVR
FMSMTGG

Sequences:

>Translated_327_residues
MNAPALVDQFGRHIDYVRLSVTDRCDLRCSYCMPEGFKGFEEPADWLDFDEIERLLGAFARLGVRRVRLTGGEPLLRRDI
SGLARRIASLPGIEDLSLSTNATQLDRHAAALKAAGVTRLNVSLDSLQQARVERINGRDVLAKVMTGLAAAQEAGFAPIK
LNMVAMRGSNDDEIDEMVAFCMDRGFVLRLIEAMPMGATGRNTEYLDLQPVKERLRARFGLVETALPGGGPARYLGTADG
GFNVGFITPISQHFCESCNRIRIAVDGTAYMCLGQDEKFEFRPLLRDGCSDAELDAAIVEAINLKPERHEFREAPHKLVR
FMSMTGG
>Mature_327_residues
MNAPALVDQFGRHIDYVRLSVTDRCDLRCSYCMPEGFKGFEEPADWLDFDEIERLLGAFARLGVRRVRLTGGEPLLRRDI
SGLARRIASLPGIEDLSLSTNATQLDRHAAALKAAGVTRLNVSLDSLQQARVERINGRDVLAKVMTGLAAAQEAGFAPIK
LNMVAMRGSNDDEIDEMVAFCMDRGFVLRLIEAMPMGATGRNTEYLDLQPVKERLRARFGLVETALPGGGPARYLGTADG
GFNVGFITPISQHFCESCNRIRIAVDGTAYMCLGQDEKFEFRPLLRDGCSDAELDAAIVEAINLKPERHEFREAPHKLVR
FMSMTGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=311, Percent_Identity=35.6913183279743, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI22027536, Length=311, Percent_Identity=35.6913183279743, Blast_Score=192, Evalue=4e-49,
Organism=Escherichia coli, GI1786999, Length=329, Percent_Identity=35.258358662614, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI32566329, Length=329, Percent_Identity=34.6504559270517, Blast_Score=195, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI32566331, Length=310, Percent_Identity=34.8387096774194, Blast_Score=194, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI17561210, Length=326, Percent_Identity=34.9693251533742, Blast_Score=190, Evalue=9e-49,
Organism=Drosophila melanogaster, GI28574490, Length=312, Percent_Identity=38.7820512820513, Blast_Score=203, Evalue=2e-52,
Organism=Drosophila melanogaster, GI28574488, Length=312, Percent_Identity=38.7820512820513, Blast_Score=202, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 36083; Mature: 36083

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS01305 MOAA_NIFB_PQQE ; PS00194 THIOREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAPALVDQFGRHIDYVRLSVTDRCDLRCSYCMPEGFKGFEEPADWLDFDEIERLLGAFA
CCCCHHHHHHCCCEEEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RLGVRRVRLTGGEPLLRRDISGLARRIASLPGIEDLSLSTNATQLDRHAAALKAAGVTRL
HHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
NVSLDSLQQARVERINGRDVLAKVMTGLAAAQEAGFAPIKLNMVAMRGSNDDEIDEMVAF
ECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHH
CMDRGFVLRLIEAMPMGATGRNTEYLDLQPVKERLRARFGLVETALPGGGPARYLGTADG
HHCCHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
GFNVGFITPISQHFCESCNRIRIAVDGTAYMCLGQDEKFEFRPLLRDGCSDAELDAAIVE
CCCEEEECHHHHHHHHCCCEEEEEECCEEEEEECCCCCCEECHHHHCCCCCCHHHHHHHH
AINLKPERHEFREAPHKLVRFMSMTGG
HHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNAPALVDQFGRHIDYVRLSVTDRCDLRCSYCMPEGFKGFEEPADWLDFDEIERLLGAFA
CCCCHHHHHHCCCEEEEEEEECCCCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RLGVRRVRLTGGEPLLRRDISGLARRIASLPGIEDLSLSTNATQLDRHAAALKAAGVTRL
HHCCEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEE
NVSLDSLQQARVERINGRDVLAKVMTGLAAAQEAGFAPIKLNMVAMRGSNDDEIDEMVAF
ECCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHH
CMDRGFVLRLIEAMPMGATGRNTEYLDLQPVKERLRARFGLVETALPGGGPARYLGTADG
HHCCHHHHHHHHHCCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCC
GFNVGFITPISQHFCESCNRIRIAVDGTAYMCLGQDEKFEFRPLLRDGCSDAELDAAIVE
CCCEEEECHHHHHHHHCCCEEEEEECCEEEEEECCCCCCEECHHHHCCCCCCHHHHHHHH
AINLKPERHEFREAPHKLVRFMSMTGG
HHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA