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The map label for this gene is gph2 [H]

Identifier: 74316963

GI number: 74316963

Start: 1005769

End: 1006449

Strand: Reverse

Name: gph2 [H]

Synonym: Tbd_0945

Alternate gene names: 74316963

Gene position: 1006449-1005769 (Counterclockwise)

Preceding gene: 74316964

Following gene: 74316962

Centisome position: 34.59

GC content: 72.69

Gene sequence:

>681_bases
GTGAAGCCTGAGCACGCGCGGCTCGACGGCATCCGCGCAGTCCTGTTCGACCTCGACGGCACGCTGGTCGACACGGCGCC
CGACCTCGGCAACGCGCTCAACCTGCAGCGCGCGCGCCACGGCCTCGCCCCGCTTGCCGCCGACGTCATCCGCCCGCAAG
CCTCGCACGGCGCGCGCGGGCTGCTCGCCCTCGGTTTCGACCTCAAGCCCGACGACCCGCGCTTCGCGGCCATGCGCGAA
GAATTCCTGCAGCTCTACGCCGACAACATCTGTCAGGCCTCGCGCCCGTTTCCCGGCGTGCCCGAGCTGCTCGACGCCCT
CGAAGCGCGCGGCTACAAATGGGGTGTCGTCACCAACAAGCCGGCGCGCTTCACTGAACCGCTGATGTCGGTGCTCGACC
TCGCCGAGCGCGCGGCCTGCATCGTCTCCGGCGACAGCTGCCCGCAGCCCAAGCCCCATCCGGCGCCCATGCTCGTCGCC
GCCCGGCGCTGCGACGCGCTACCCGCGCAATGCCTCTACCTCGGCGACGCCGAGCGTGACGTCCAGGCCGCGACCGCCGC
GGGCATGCCCGCGCTCGTCGCCGCCTGGGGCTATCTGGGCGCCGAGGACGCGCCGCACGCCTGGGGCGCGCACGCGCAGA
TCCACCACCCGCTCGACACGCTCGACTATCTGCCTGCCTGA

Upstream 100 bases:

>100_bases
CTCGAAACCGAGGAGCTGACCGGCATGACCTACAACCCGCTGACCAAGGTTTACCGGCTCGAAGCCGACACCGACGTCAA
CTACCTGATGGCGACCCGCC

Downstream 100 bases:

>100_bases
CCGTGGCCTACACGCTGATCAAACAGATCCATCTCGCCACGATCGCGATCACGCTCGCGCTCTTCCTCTTGCGCGGCTTC
TGGATGATGGCCGAGTCGAA

Product: putative 2-phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKPEHARLDGIRAVLFDLDGTLVDTAPDLGNALNLQRARHGLAPLAADVIRPQASHGARGLLALGFDLKPDDPRFAAMRE
EFLQLYADNICQASRPFPGVPELLDALEARGYKWGVVTNKPARFTEPLMSVLDLAERAACIVSGDSCPQPKPHPAPMLVA
ARRCDALPAQCLYLGDAERDVQAATAAGMPALVAAWGYLGAEDAPHAWGAHAQIHHPLDTLDYLPA

Sequences:

>Translated_226_residues
MKPEHARLDGIRAVLFDLDGTLVDTAPDLGNALNLQRARHGLAPLAADVIRPQASHGARGLLALGFDLKPDDPRFAAMRE
EFLQLYADNICQASRPFPGVPELLDALEARGYKWGVVTNKPARFTEPLMSVLDLAERAACIVSGDSCPQPKPHPAPMLVA
ARRCDALPAQCLYLGDAERDVQAATAAGMPALVAAWGYLGAEDAPHAWGAHAQIHHPLDTLDYLPA
>Mature_226_residues
MKPEHARLDGIRAVLFDLDGTLVDTAPDLGNALNLQRARHGLAPLAADVIRPQASHGARGLLALGFDLKPDDPRFAAMRE
EFLQLYADNICQASRPFPGVPELLDALEARGYKWGVVTNKPARFTEPLMSVLDLAERAACIVSGDSCPQPKPHPAPMLVA
ARRCDALPAQCLYLGDAERDVQAATAAGMPALVAAWGYLGAEDAPHAWGAHAQIHHPLDTLDYLPA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=214, Percent_Identity=35.981308411215, Blast_Score=117, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 24247; Mature: 24247

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPEHARLDGIRAVLFDLDGTLVDTAPDLGNALNLQRARHGLAPLAADVIRPQASHGARG
CCCCHHHHHHHHHHEECCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCE
LLALGFDLKPDDPRFAAMREEFLQLYADNICQASRPFPGVPELLDALEARGYKWGVVTNK
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCC
PARFTEPLMSVLDLAERAACIVSGDSCPQPKPHPAPMLVAARRCDALPAQCLYLGDAERD
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHH
VQAATAAGMPALVAAWGYLGAEDAPHAWGAHAQIHHPLDTLDYLPA
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCHHHHHCCCC
>Mature Secondary Structure
MKPEHARLDGIRAVLFDLDGTLVDTAPDLGNALNLQRARHGLAPLAADVIRPQASHGARG
CCCCHHHHHHHHHHEECCCCCEEECCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCE
LLALGFDLKPDDPRFAAMREEFLQLYADNICQASRPFPGVPELLDALEARGYKWGVVTNK
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCEEEEECCC
PARFTEPLMSVLDLAERAACIVSGDSCPQPKPHPAPMLVAARRCDALPAQCLYLGDAERD
CHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEECCCCHH
VQAATAAGMPALVAAWGYLGAEDAPHAWGAHAQIHHPLDTLDYLPA
HHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]