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The map label for this gene is lysS

Identifier: 74316911

GI number: 74316911

Start: 942698

End: 944203

Strand: Reverse

Name: lysS

Synonym: Tbd_0893

Alternate gene names: 74316911

Gene position: 944203-942698 (Counterclockwise)

Preceding gene: 74316912

Following gene: 74316910

Centisome position: 32.45

GC content: 65.41

Gene sequence:

>1506_bases
ATGACCGACCCCACCAACGCCCCCGCCCTCGACGAAAACCAGATCATCGCCGAGCGCCGCGCCAAGCTCGCCGCGATCCG
CGAGGCGGGCGTCGCCTTCCCCAACGACTTCGAGCGCCGCGACTACGCCGGCAGGCTCGCGCACGAACACGGCGACAAAT
CCAAGGAAGCGCTCGAAGCCGAGGCCGTCGACGTGCAGCTGGCGGGCCGCATGATGCTCAAGCGCGTGATGGGCAAGGCG
AGCTTCGCGACGCTGCAGGACATGAGCGGCCGCATCCAGGTCTACGTCTCGAACGACCTGACCGGGCTCGACGCGCACGA
GGCGTTCAAGCGCTGGGACCTCGGCGACTTCGTCGGCGTGAGCGGCACGCTGTTCAAGACCAACAAGGGCGAACTGACGA
TCCAGGCAAAATCGGTCCGCTTGCTGTCGAAGGCGCTGCGTCCGCTGCCGGAAAAATTCCACGGGCTCGCGGACCAGGAA
CAGAAGTACCGCCAGCGCTATCTCGACCTCATCACCAACGACGACGCGCGGTCGACCTTCGTGCGCCGCTCGAAGATCAT
CCAGGCGATCCGCGAATTCATGACCGGCCACGGCTTTCTCGAAGTCGAGACGCCGATGATGCACCCGATCCCCGGCGGCG
CCGCGGCCAAGCCCTTCGTCACCCACCACAACGCGCTCGACATGGAGCTCTTCCTGCGCATCGCGCCCGAGCTCTATCTC
AAGCGGCTCGTCGTCGGCGGCTTCGAGAAGGTGTTCGAGATCAACCGCAACTTCCGCAACGAGGGCTTGAGCACGCGCCA
CAACCCCGAGTTCACGATGATGGAGTTCTACGAGGCCTACCGCGAATATCGCTACCTCATGGACTTCACCGAGGCGCTGA
TCCGCCACACCGCGGTCGCCGCGACCGGCTCGACGACGATTTCCTACCAGGGAAGCACGATCGAACTCGGCACGCCCTTC
GACCGCCTGACCATCGTCGAGGCGGTGCGCAAATACCACCCCGAGTACACCGTCGAGCAACTCAACGATCGCGACTGGCT
GACCGCGCAATTCACGGCGATGAAGGCCAAGTACCGCGAGCACGACGGGCTCGGCGGCCTGCAGCTGACCTTCTTCGAGG
AGACGACCGAGGCGCTGCTCGTGCAGCCGACCTTCATTGTCGACTACCCGGCCGAAGTCTCGCCGCTGGCGCGTCGCTCC
GACACGCAGCCCGAGATCACCGAGCGCTTCGAACTCTTCATCACCGGCCGTGAAATGGCGAACGGCTTTTCCGAATTGAA
CGACGCCGAGGACCAGGCCGAACGCTTCATGGAGCAGGTGCGCGCCAAGGAAGCCGGCGACGAGGAGGCGATGCACTACG
ACGCCGACTTCATCCGCGCGCTCGAACACGGCCTGCCGCCGACCGGCGGCTGCGGCATCGGCATCGACCGCCTCGTGATG
CTGCTCACCGATTCGCCGTCGATCCGCGACGTCATCCTCTTCCCGCAGATGCGGCGCGAGGCCTGA

Upstream 100 bases:

>100_bases
GCCATTACGGTGCAGTCAGTTTCGCCACCGCCGACAAGCATCAAGCCGACGCGGCCAGAGCGCTCGGTATCCCCGTTTTC
ACCTTTTATTGAATGGCATC

Downstream 100 bases:

>100_bases
GCCGGGCTCGCCGGCGGCGCGGCTGAAGCAGACATGGGCCAATATCTGCTCAAGATCGCCCTCTCGGCGTTCGTCGTCGT
CGCGGTCGCCGAGATCGCCA

Product: lysyl-tRNA synthetase, class-2

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTDPTNAPALDENQIIAERRAKLAAIREAGVAFPNDFERRDYAGRLAHEHGDKSKEALEAEAVDVQLAGRMMLKRVMGKA
SFATLQDMSGRIQVYVSNDLTGLDAHEAFKRWDLGDFVGVSGTLFKTNKGELTIQAKSVRLLSKALRPLPEKFHGLADQE
QKYRQRYLDLITNDDARSTFVRRSKIIQAIREFMTGHGFLEVETPMMHPIPGGAAAKPFVTHHNALDMELFLRIAPELYL
KRLVVGGFEKVFEINRNFRNEGLSTRHNPEFTMMEFYEAYREYRYLMDFTEALIRHTAVAATGSTTISYQGSTIELGTPF
DRLTIVEAVRKYHPEYTVEQLNDRDWLTAQFTAMKAKYREHDGLGGLQLTFFEETTEALLVQPTFIVDYPAEVSPLARRS
DTQPEITERFELFITGREMANGFSELNDAEDQAERFMEQVRAKEAGDEEAMHYDADFIRALEHGLPPTGGCGIGIDRLVM
LLTDSPSIRDVILFPQMRREA

Sequences:

>Translated_501_residues
MTDPTNAPALDENQIIAERRAKLAAIREAGVAFPNDFERRDYAGRLAHEHGDKSKEALEAEAVDVQLAGRMMLKRVMGKA
SFATLQDMSGRIQVYVSNDLTGLDAHEAFKRWDLGDFVGVSGTLFKTNKGELTIQAKSVRLLSKALRPLPEKFHGLADQE
QKYRQRYLDLITNDDARSTFVRRSKIIQAIREFMTGHGFLEVETPMMHPIPGGAAAKPFVTHHNALDMELFLRIAPELYL
KRLVVGGFEKVFEINRNFRNEGLSTRHNPEFTMMEFYEAYREYRYLMDFTEALIRHTAVAATGSTTISYQGSTIELGTPF
DRLTIVEAVRKYHPEYTVEQLNDRDWLTAQFTAMKAKYREHDGLGGLQLTFFEETTEALLVQPTFIVDYPAEVSPLARRS
DTQPEITERFELFITGREMANGFSELNDAEDQAERFMEQVRAKEAGDEEAMHYDADFIRALEHGLPPTGGCGIGIDRLVM
LLTDSPSIRDVILFPQMRREA
>Mature_500_residues
TDPTNAPALDENQIIAERRAKLAAIREAGVAFPNDFERRDYAGRLAHEHGDKSKEALEAEAVDVQLAGRMMLKRVMGKAS
FATLQDMSGRIQVYVSNDLTGLDAHEAFKRWDLGDFVGVSGTLFKTNKGELTIQAKSVRLLSKALRPLPEKFHGLADQEQ
KYRQRYLDLITNDDARSTFVRRSKIIQAIREFMTGHGFLEVETPMMHPIPGGAAAKPFVTHHNALDMELFLRIAPELYLK
RLVVGGFEKVFEINRNFRNEGLSTRHNPEFTMMEFYEAYREYRYLMDFTEALIRHTAVAATGSTTISYQGSTIELGTPFD
RLTIVEAVRKYHPEYTVEQLNDRDWLTAQFTAMKAKYREHDGLGGLQLTFFEETTEALLVQPTFIVDYPAEVSPLARRSD
TQPEITERFELFITGREMANGFSELNDAEDQAERFMEQVRAKEAGDEEAMHYDADFIRALEHGLPPTGGCGIGIDRLVML
LTDSPSIRDVILFPQMRREA

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI5031815, Length=522, Percent_Identity=41.1877394636015, Blast_Score=360, Evalue=1e-99,
Organism=Homo sapiens, GI194272210, Length=522, Percent_Identity=41.1877394636015, Blast_Score=360, Evalue=2e-99,
Organism=Homo sapiens, GI40789249, Length=282, Percent_Identity=25.177304964539, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789256, Length=491, Percent_Identity=60.4887983706721, Blast_Score=615, Evalue=1e-177,
Organism=Escherichia coli, GI1790571, Length=492, Percent_Identity=60.9756097560976, Blast_Score=614, Evalue=1e-177,
Organism=Escherichia coli, GI87082379, Length=329, Percent_Identity=31.9148936170213, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1788173, Length=305, Percent_Identity=26.5573770491803, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17535925, Length=518, Percent_Identity=39.5752895752896, Blast_Score=335, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17535927, Length=518, Percent_Identity=39.5752895752896, Blast_Score=334, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI71994340, Length=506, Percent_Identity=40.1185770750988, Blast_Score=333, Evalue=8e-92,
Organism=Caenorhabditis elegans, GI32566633, Length=211, Percent_Identity=26.5402843601896, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=522, Percent_Identity=40.2298850574713, Blast_Score=347, Evalue=2e-96,
Organism=Saccharomyces cerevisiae, GI6324256, Length=385, Percent_Identity=32.2077922077922, Blast_Score=172, Evalue=1e-43,
Organism=Drosophila melanogaster, GI24640849, Length=521, Percent_Identity=41.4587332053743, Blast_Score=353, Evalue=1e-97,
Organism=Drosophila melanogaster, GI24640851, Length=521, Percent_Identity=41.4587332053743, Blast_Score=353, Evalue=2e-97,
Organism=Drosophila melanogaster, GI24584738, Length=332, Percent_Identity=28.3132530120482, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYK_THIDA (Q3SKD9)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314651.1
- ProteinModelPortal:   Q3SKD9
- SMR:   Q3SKD9
- STRING:   Q3SKD9
- GeneID:   3672684
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0893
- NMPDR:   fig|292415.3.peg.402
- eggNOG:   COG1190
- HOGENOM:   HBG631383
- OMA:   VDMIANP
- PhylomeDB:   Q3SKD9
- BioCyc:   TDEN292415:TBD_0893-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00252
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF4
- PRINTS:   PR00982
- TIGRFAMs:   TIGR00499

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.6

Molecular weight: Translated: 56921; Mature: 56790

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPTNAPALDENQIIAERRAKLAAIREAGVAFPNDFERRDYAGRLAHEHGDKSKEALEA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
EAVDVQLAGRMMLKRVMGKASFATLQDMSGRIQVYVSNDLTGLDAHEAFKRWDLGDFVGV
HHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCC
SGTLFKTNKGELTIQAKSVRLLSKALRPLPEKFHGLADQEQKYRQRYLDLITNDDARSTF
CCEEEECCCCCEEEEHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHH
VRRSKIIQAIREFMTGHGFLEVETPMMHPIPGGAAAKPFVTHHNALDMELFLRIAPELYL
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
KRLVVGGFEKVFEINRNFRNEGLSTRHNPEFTMMEFYEAYREYRYLMDFTEALIRHTAVA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
ATGSTTISYQGSTIELGTPFDRLTIVEAVRKYHPEYTVEQLNDRDWLTAQFTAMKAKYRE
ECCCEEEEECCCEEECCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCEEHHHHHHHHHHHH
HDGLGGLQLTFFEETTEALLVQPTFIVDYPAEVSPLARRSDTQPEITERFELFITGREMA
CCCCCCEEEEEECCCCHHEEECCEEEEECCCCCCHHHHCCCCCCHHHHHEEEEEECHHHH
NGFSELNDAEDQAERFMEQVRAKEAGDEEAMHYDADFIRALEHGLPPTGGCGIGIDRLVM
HHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEE
LLTDSPSIRDVILFPQMRREA
EECCCCCCCEEEECCHHHHCC
>Mature Secondary Structure 
TDPTNAPALDENQIIAERRAKLAAIREAGVAFPNDFERRDYAGRLAHEHGDKSKEALEA
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
EAVDVQLAGRMMLKRVMGKASFATLQDMSGRIQVYVSNDLTGLDAHEAFKRWDLGDFVGV
HHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCCCCC
SGTLFKTNKGELTIQAKSVRLLSKALRPLPEKFHGLADQEQKYRQRYLDLITNDDARSTF
CCEEEECCCCCEEEEHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHCCCCHHHHH
VRRSKIIQAIREFMTGHGFLEVETPMMHPIPGGAAAKPFVTHHNALDMELFLRIAPELYL
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHH
KRLVVGGFEKVFEINRNFRNEGLSTRHNPEFTMMEFYEAYREYRYLMDFTEALIRHTAVA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
ATGSTTISYQGSTIELGTPFDRLTIVEAVRKYHPEYTVEQLNDRDWLTAQFTAMKAKYRE
ECCCEEEEECCCEEECCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCEEHHHHHHHHHHHH
HDGLGGLQLTFFEETTEALLVQPTFIVDYPAEVSPLARRSDTQPEITERFELFITGREMA
CCCCCCEEEEEECCCCHHEEECCEEEEECCCCCCHHHHCCCCCCHHHHHEEEEEECHHHH
NGFSELNDAEDQAERFMEQVRAKEAGDEEAMHYDADFIRALEHGLPPTGGCGIGIDRLVM
HHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHEE
LLTDSPSIRDVILFPQMRREA
EECCCCCCCEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA