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The map label for this gene is hcaE [H]

Identifier: 74316900

GI number: 74316900

Start: 931817

End: 932929

Strand: Direct

Name: hcaE [H]

Synonym: Tbd_0882

Alternate gene names: 74316900

Gene position: 931817-932929 (Clockwise)

Preceding gene: 74316895

Following gene: 74316901

Centisome position: 32.02

GC content: 64.42

Gene sequence:

>1113_bases
GTGAGCGATCTCGCCGAATCCAGCGCCTTGTCGCTAACTTCGCCTCAACTGCCAGTTTCCTGGTACTTCGATCCGGTCAT
CTACGACCTGGAATTGACCCATCTATTCAAGCGCGGGCCGGGCTACGCCGGGCACCAGCTCATGGTGCCCGAGCCGGGCG
ACTATCATGCGCTCGAAATGTTCGACGGCGGCAAGATGCTCGTCAACAGCGGATCGGGTGTCGAACTCCTCTCTAACGTC
TGCCGCCATCGCCAGGCGCTGATGCTCACGGGCCGCGGCAAGCTGCCCTCCGACAACATCGTCTGCCCGGTGCATCGCTG
GACCTACGATCGGCAGGGCCAACTGCTGGGTGCGCCGGAGTTTCCCGGCAACCCCTGTCTGAACCTCGGCCGGACCGGCC
TGCAGAACTGGCAGGGACTGCTGTTCGCCGGCGAGCGCGACGTCAAGGCCGATCTCGCCGGCATGCAGGTCGCGCGCGAA
CTGGACTTTTCCGGATTCATGCTCGACCGCGTGCAGGTGACGCATTACGCATGCAACTGGAAGACCTTCATCGAGGTTTA
CCTCGAGGACTACCACGTCGCCCCCTATCACCCGGGCCTCGGTCACTTCGTCACCTGCGACGACCTCAAGTGGGAATACG
GAGACTGGTGGTCGGTGCAGACCGTGGGCGTGAACCGCGGCCTCGCCAAGCCGGGCTCGCCGGCCTACCGGAAATGGCAC
GAGCAGGTGCTGGCCTATGATCGCGGCGCGATCCCGGCGCACGGCTCGATCTGGCTGACCTACTATCCCGGCCTCATGGT
CGAGTGGTACCCGCACGTGCTCGTCGTGTCGTCGATCGTGCCGACCGGCGTCGAGTCGTGCAGCAACGTCGTCGAGTTCT
ACTACCCGGAGGACATCGTGCTGTTCGAGCGTGCCTTCGTCGAGGCCGAGCAGGCCGCGTACGAGGAAACGGCAGTCGAG
GACGAGGACATCTGCGCGCGTATGCACGCCGGGCGGCGCGCGCTGTACAAGCAGGGAATCTCGGAGACCGGCCCCTACCA
GTCGCCGCTCGAGGACGGCATGATGCATTTCCATGCCTTCCTGCGGCGCGAGATCGAACCTCACCTCGGATGA

Upstream 100 bases:

>100_bases
CCCTGGCTCGGAAGCCGGGGGTTCACCCGGTAAATCCGGTGGGTCACACTTGCCGCTCCGGGAATTCCTGGAGGGGGCTC
GGTTCTGAGTAGGGTACGCC

Downstream 100 bases:

>100_bases
GCGCACGCACGAACCCGGACGGGCGGCCGTTGCCGCCCGTTTTTTTCGTCCAGGCGACATGACGTCGGGCTGGATGCTCG
TCGCGGCGGCGCTGTTCGCG

Product: Rieske iron-sulfur protein 2Fe-2S subunit

Products: NA

Alternate protein names: Digoxigenin subunit alpha [H]

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MSDLAESSALSLTSPQLPVSWYFDPVIYDLELTHLFKRGPGYAGHQLMVPEPGDYHALEMFDGGKMLVNSGSGVELLSNV
CRHRQALMLTGRGKLPSDNIVCPVHRWTYDRQGQLLGAPEFPGNPCLNLGRTGLQNWQGLLFAGERDVKADLAGMQVARE
LDFSGFMLDRVQVTHYACNWKTFIEVYLEDYHVAPYHPGLGHFVTCDDLKWEYGDWWSVQTVGVNRGLAKPGSPAYRKWH
EQVLAYDRGAIPAHGSIWLTYYPGLMVEWYPHVLVVSSIVPTGVESCSNVVEFYYPEDIVLFERAFVEAEQAAYEETAVE
DEDICARMHAGRRALYKQGISETGPYQSPLEDGMMHFHAFLRREIEPHLG

Sequences:

>Translated_370_residues
MSDLAESSALSLTSPQLPVSWYFDPVIYDLELTHLFKRGPGYAGHQLMVPEPGDYHALEMFDGGKMLVNSGSGVELLSNV
CRHRQALMLTGRGKLPSDNIVCPVHRWTYDRQGQLLGAPEFPGNPCLNLGRTGLQNWQGLLFAGERDVKADLAGMQVARE
LDFSGFMLDRVQVTHYACNWKTFIEVYLEDYHVAPYHPGLGHFVTCDDLKWEYGDWWSVQTVGVNRGLAKPGSPAYRKWH
EQVLAYDRGAIPAHGSIWLTYYPGLMVEWYPHVLVVSSIVPTGVESCSNVVEFYYPEDIVLFERAFVEAEQAAYEETAVE
DEDICARMHAGRRALYKQGISETGPYQSPLEDGMMHFHAFLRREIEPHLG
>Mature_369_residues
SDLAESSALSLTSPQLPVSWYFDPVIYDLELTHLFKRGPGYAGHQLMVPEPGDYHALEMFDGGKMLVNSGSGVELLSNVC
RHRQALMLTGRGKLPSDNIVCPVHRWTYDRQGQLLGAPEFPGNPCLNLGRTGLQNWQGLLFAGERDVKADLAGMQVAREL
DFSGFMLDRVQVTHYACNWKTFIEVYLEDYHVAPYHPGLGHFVTCDDLKWEYGDWWSVQTVGVNRGLAKPGSPAYRKWHE
QVLAYDRGAIPAHGSIWLTYYPGLMVEWYPHVLVVSSIVPTGVESCSNVVEFYYPEDIVLFERAFVEAEQAAYEETAVED
EDICARMHAGRRALYKQGISETGPYQSPLEDGMMHFHAFLRREIEPHLG

Specific function: Part of the multicomponent 3-phenylpropionate dioxygenase. Converts 3-phenylpropionic acid (PP) and cinnamic acid (CI) into 3-phenylpropionate-dihydrodiol (PP-dihydrodiol) and cinnamic acid-dihydrodiol (CI-dihydrodiol), respectively [H]

COG id: COG4638

COG function: function code PR; Phenylpropionate dioxygenase and related ring-hydroxylating dioxygenases, large terminal subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Rieske domain [H]

Homologues:

Organism=Escherichia coli, GI1788103, Length=364, Percent_Identity=22.8021978021978, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788888, Length=217, Percent_Identity=27.6497695852535, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020875
- InterPro:   IPR017941
- InterPro:   IPR015881
- InterPro:   IPR015879
- InterPro:   IPR001663 [H]

Pfam domain/function: PF00355 Rieske; PF00848 Ring_hydroxyl_A [H]

EC number: =1.14.12.19 [H]

Molecular weight: Translated: 41777; Mature: 41646

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLAESSALSLTSPQLPVSWYFDPVIYDLELTHLFKRGPGYAGHQLMVPEPGDYHALEM
CCCCCCCCCCEECCCCCCEEEEECCEEEEHHHHHHHHCCCCCCCCEEECCCCCCCEEEEE
FDGGKMLVNSGSGVELLSNVCRHRQALMLTGRGKLPSDNIVCPVHRWTYDRQGQLLGAPE
ECCCEEEEECCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEECHHHCCCCCCCCEEECCC
FPGNPCLNLGRTGLQNWQGLLFAGERDVKADLAGMQVARELDFSGFMLDRVQVTHYACNW
CCCCCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCH
KTFIEVYLEDYHVAPYHPGLGHFVTCDDLKWEYGDWWSVQTVGVNRGLAKPGSPAYRKWH
HHHHHHHHHHCEECCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHH
EQVLAYDRGAIPAHGSIWLTYYPGLMVEWYPHVLVVSSIVPTGVESCSNVVEFYYPEDIV
HHHHHHCCCCCCCCCCEEEEECCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEE
LFERAFVEAEQAAYEETAVEDEDICARMHAGRRALYKQGISETGPYQSPLEDGMMHFHAF
EHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
LRREIEPHLG
HHHHCCCCCC
>Mature Secondary Structure 
SDLAESSALSLTSPQLPVSWYFDPVIYDLELTHLFKRGPGYAGHQLMVPEPGDYHALEM
CCCCCCCCCEECCCCCCEEEEECCEEEEHHHHHHHHCCCCCCCCEEECCCCCCCEEEEE
FDGGKMLVNSGSGVELLSNVCRHRQALMLTGRGKLPSDNIVCPVHRWTYDRQGQLLGAPE
ECCCEEEEECCCCHHHHHHHHHCCEEEEEECCCCCCCCCEEECHHHCCCCCCCCEEECCC
FPGNPCLNLGRTGLQNWQGLLFAGERDVKADLAGMQVARELDFSGFMLDRVQVTHYACNW
CCCCCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCH
KTFIEVYLEDYHVAPYHPGLGHFVTCDDLKWEYGDWWSVQTVGVNRGLAKPGSPAYRKWH
HHHHHHHHHHCEECCCCCCCCCEEEECCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHH
EQVLAYDRGAIPAHGSIWLTYYPGLMVEWYPHVLVVSSIVPTGVESCSNVVEFYYPEDIV
HHHHHHCCCCCCCCCCEEEEECCCEEEECCCHHHHHHHHCCCCHHHHHHHHHHHCCCCEE
LFERAFVEAEQAAYEETAVEDEDICARMHAGRRALYKQGISETGPYQSPLEDGMMHFHAF
EHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
LRREIEPHLG
HHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]