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The map label for this gene is def [H]

Identifier: 74316798

GI number: 74316798

Start: 823656

End: 824189

Strand: Direct

Name: def [H]

Synonym: Tbd_0780

Alternate gene names: 74316798

Gene position: 823656-824189 (Clockwise)

Preceding gene: 74316797

Following gene: 74316799

Centisome position: 28.31

GC content: 68.54

Gene sequence:

>534_bases
ATGGCCGTCCGCGAAGTCCTGAAAATGGGAGACCCCCGGCTGCTCGCGCCGGCCCGCGCGGTCGAGCGTTTCGCCACCGC
GGAACTCGCGCAGCTCGTCGCCGACATGCACGACACCATGCGCGCGCTGAACGGCGCGGGCCTCGCCGCGCCGCAGATCG
GCGTGTCGCTGCAGGTCGTGATCTTCGAGGTGAACGCCAACCCGCGCTACCCCGACGCCGCCGAGGTTCCGTTGACCGTT
CTGGTCAACCCGGTGGTCACGCCGCTCTCCGACGCGACCGAGGAAGGCTGGGAGGGCTGCCTGTCGGTACCCGGCATGCG
TGGACTGGTGCCGCGCCACGGCGAAATCCACTATCGCGGGTTCGACGCGGCCGGGCGTGCGCTCGAGCGCCGGGTGAGCG
GTTTTCACGCCCGCGTCGTGCAGCACGAAGTCGACCACCTGAATGGCATCCTGTATCCGATGCGGATCCCGGACCTGCGC
AATTTCGGTTTTACCGACACCCTGTTTCCCGGGCAGACGCTGCAGGACGACTGA

Upstream 100 bases:

>100_bases
TGCATCGCGAAGCGGTCGGCGAGTATCCGCTGCCGGACGATCTGCCGCCGGGCGGCTGGCGCTGGCTCGAAGCCGGCGAT
CTGCAACGACTGGAGTGTGC

Downstream 100 bases:

>100_bases
CTACCCCCTTGTTTTGGCAGGCATTTGGCCGACGGCGACCGGCCTAAAGTTGTCGCCGGGGGCGCCGCTAACTTGTTACG
TAACCGTGGGCGAGGTGTCC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MAVREVLKMGDPRLLAPARAVERFATAELAQLVADMHDTMRALNGAGLAAPQIGVSLQVVIFEVNANPRYPDAAEVPLTV
LVNPVVTPLSDATEEGWEGCLSVPGMRGLVPRHGEIHYRGFDAAGRALERRVSGFHARVVQHEVDHLNGILYPMRIPDLR
NFGFTDTLFPGQTLQDD

Sequences:

>Translated_177_residues
MAVREVLKMGDPRLLAPARAVERFATAELAQLVADMHDTMRALNGAGLAAPQIGVSLQVVIFEVNANPRYPDAAEVPLTV
LVNPVVTPLSDATEEGWEGCLSVPGMRGLVPRHGEIHYRGFDAAGRALERRVSGFHARVVQHEVDHLNGILYPMRIPDLR
NFGFTDTLFPGQTLQDD
>Mature_176_residues
AVREVLKMGDPRLLAPARAVERFATAELAQLVADMHDTMRALNGAGLAAPQIGVSLQVVIFEVNANPRYPDAAEVPLTVL
VNPVVTPLSDATEEGWEGCLSVPGMRGLVPRHGEIHYRGFDAAGRALERRVSGFHARVVQHEVDHLNGILYPMRIPDLRN
FGFTDTLFPGQTLQDD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=34.2857142857143, Blast_Score=84, Evalue=9e-17,
Organism=Escherichia coli, GI1789682, Length=153, Percent_Identity=37.9084967320261, Blast_Score=93, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=38.4615384615385, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24645728, Length=171, Percent_Identity=33.3333333333333, Blast_Score=95, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19344; Mature: 19213

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVREVLKMGDPRLLAPARAVERFATAELAQLVADMHDTMRALNGAGLAAPQIGVSLQVV
CCHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
IFEVNANPRYPDAAEVPLTVLVNPVVTPLSDATEEGWEGCLSVPGMRGLVPRHGEIHYRG
EEEECCCCCCCCCCCCCCEEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
FDAAGRALERRVSGFHARVVQHEVDHLNGILYPMRIPDLRNFGFTDTLFPGQTLQDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AVREVLKMGDPRLLAPARAVERFATAELAQLVADMHDTMRALNGAGLAAPQIGVSLQVV
CHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEE
IFEVNANPRYPDAAEVPLTVLVNPVVTPLSDATEEGWEGCLSVPGMRGLVPRHGEIHYRG
EEEECCCCCCCCCCCCCCEEEECCHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
FDAAGRALERRVSGFHARVVQHEVDHLNGILYPMRIPDLRNFGFTDTLFPGQTLQDD
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]