Click here to switch to the map view.

The map label for this gene is rsuA [H]

Identifier: 74316797

GI number: 74316797

Start: 822955

End: 823677

Strand: Direct

Name: rsuA [H]

Synonym: Tbd_0779

Alternate gene names: 74316797

Gene position: 822955-823677 (Clockwise)

Preceding gene: 74316796

Following gene: 74316798

Centisome position: 28.28

GC content: 69.02

Gene sequence:

>723_bases
ATGAAGCTCTATCGTGCGCTGCAGTCGCAGGGGCTCGGCAGCCGCCGCGGCTGCGCGGCGCTTGTGCGCGCCGGCGCGGT
TCTCGCCAACGGACTCGCGTGCGCCGACCCTGAGGCCGAGGTCGACCCGGTCGGTCTCGAACTGACCGTGGACGGCACGG
TGTGGGCGTACCGCGACAAGGCCTACCTCGTGATGCACAAGCCGGAAGGCTACGAATGTTCGCATCATCCGCGCCACCAC
CCGAGCGTCTTCAGCCTGTTGCCTGCGCCGCTCGTGCAACGCGGCGTGCAGTGCGTCGGGCGGCTCGATCAGGACACCAC
CGGCTTGTTGCTGTTTTCCGACGACGGCCAGTTCATCCACCGCATGACCTCGCCGAAAAAGCGCGTCGGCAAGGTGTATC
GCGCGCGCTGTGCGGAGCCGGTGAACGCAGCGACGCTCGCCGCGCTGCGTGCCGGCGTGCAACTCAACGACGAGCCGGCG
CCGCTTGCGGCGCTGGCCTGCGAGGCGGTCGACGACCGTACGCTGCGTCTCGTGCTCGCCGAAGGCAAATACCATCAGGT
CAGGCGCATGATCGCCGCGGCGGGCAATCACGTCGCGCGGCTGCATCGCGAAGCGGTCGGCGAGTATCCGCTGCCGGACG
ATCTGCCGCCGGGCGGCTGGCGCTGGCTCGAAGCCGGCGATCTGCAACGACTGGAGTGTGCATGGCCGTCCGCGAAGTCC
TGA

Upstream 100 bases:

>100_bases
CTTGGCGAGGTCATGCTGCAGGTGCGTCATCTCCTGGAACAGCTCGTGACCAACGACTCGGCCGGACTCTGCACCTTCTG
AAAGCAGGATTGCCGGCGGA

Downstream 100 bases:

>100_bases
AAATGGGAGACCCCCGGCTGCTCGCGCCGGCCCGCGCGGTCGAGCGTTTCGCCACCGCGGAACTCGCGCAGCTCGTCGCC
GACATGCACGACACCATGCG

Product: ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MKLYRALQSQGLGSRRGCAALVRAGAVLANGLACADPEAEVDPVGLELTVDGTVWAYRDKAYLVMHKPEGYECSHHPRHH
PSVFSLLPAPLVQRGVQCVGRLDQDTTGLLLFSDDGQFIHRMTSPKKRVGKVYRARCAEPVNAATLAALRAGVQLNDEPA
PLAALACEAVDDRTLRLVLAEGKYHQVRRMIAAAGNHVARLHREAVGEYPLPDDLPPGGWRWLEAGDLQRLECAWPSAKS

Sequences:

>Translated_240_residues
MKLYRALQSQGLGSRRGCAALVRAGAVLANGLACADPEAEVDPVGLELTVDGTVWAYRDKAYLVMHKPEGYECSHHPRHH
PSVFSLLPAPLVQRGVQCVGRLDQDTTGLLLFSDDGQFIHRMTSPKKRVGKVYRARCAEPVNAATLAALRAGVQLNDEPA
PLAALACEAVDDRTLRLVLAEGKYHQVRRMIAAAGNHVARLHREAVGEYPLPDDLPPGGWRWLEAGDLQRLECAWPSAKS
>Mature_240_residues
MKLYRALQSQGLGSRRGCAALVRAGAVLANGLACADPEAEVDPVGLELTVDGTVWAYRDKAYLVMHKPEGYECSHHPRHH
PSVFSLLPAPLVQRGVQCVGRLDQDTTGLLLFSDDGQFIHRMTSPKKRVGKVYRARCAEPVNAATLAALRAGVQLNDEPA
PLAALACEAVDDRTLRLVLAEGKYHQVRRMIAAAGNHVARLHREAVGEYPLPDDLPPGGWRWLEAGDLQRLECAWPSAKS

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=230, Percent_Identity=36.5217391304348, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1787524, Length=232, Percent_Identity=37.0689655172414, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI87081838, Length=147, Percent_Identity=37.4149659863946, Blast_Score=83, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 26168; Mature: 26168

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLYRALQSQGLGSRRGCAALVRAGAVLANGLACADPEAEVDPVGLELTVDGTVWAYRDK
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCEEEEEECC
AYLVMHKPEGYECSHHPRHHPSVFSLLPAPLVQRGVQCVGRLDQDTTGLLLFSDDGQFIH
EEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHH
RMTSPKKRVGKVYRARCAEPVNAATLAALRAGVQLNDEPAPLAALACEAVDDRTLRLVLA
HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCEECCCCCCHHHHHHHHCCCCEEEEEEE
EGKYHQVRRMIAAAGNHVARLHREAVGEYPLPDDLPPGGWRWLEAGDLQRLECAWPSAKS
CCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCC
>Mature Secondary Structure
MKLYRALQSQGLGSRRGCAALVRAGAVLANGLACADPEAEVDPVGLELTVDGTVWAYRDK
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCEEEEEECC
AYLVMHKPEGYECSHHPRHHPSVFSLLPAPLVQRGVQCVGRLDQDTTGLLLFSDDGQFIH
EEEEEECCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHH
RMTSPKKRVGKVYRARCAEPVNAATLAALRAGVQLNDEPAPLAALACEAVDDRTLRLVLA
HHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCEECCCCCCHHHHHHHHCCCCEEEEEEE
EGKYHQVRRMIAAAGNHVARLHREAVGEYPLPDDLPPGGWRWLEAGDLQRLECAWPSAKS
CCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]