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The map label for this gene is tilS [H]

Identifier: 74316607

GI number: 74316607

Start: 617863

End: 619200

Strand: Direct

Name: tilS [H]

Synonym: Tbd_0589

Alternate gene names: 74316607

Gene position: 617863-619200 (Clockwise)

Preceding gene: 74316606

Following gene: 74316608

Centisome position: 21.23

GC content: 73.09

Gene sequence:

>1338_bases
GTGGGCGACGCGCTCGCCCGCCATGTCTTCTCCGGCGCGCGGCTCGTGCTCGCGCTGTCGGGCGGCCTCGATTCGGTGGT
TCTGCTGCACGTGCTCGCGTCCCTGCGCGGGCGACACCGCTTCGCGCTCGCCGCCGTTCATGTCCACCATGGCCTCTCGC
CGCATGCAAGCGACTGGGCCGATTTTTGCGAGCGGCTCTGTGCCGCCCACGCGCTCGACCTGCAGGTCGAGCGCGTGCAC
ATCGCCGGCGACGACCCGGCTGGCACCGAGGCCGCGGCCCGCCGGGAACGACATCGCGTCTTCGCGCGCCTCGACGCGGA
TTTCGTGCTGACTGCGCACCACCTGGACGATCAGGCCGAAACCGTGCTCCTTCAACTGTTGCGCGGCGCCGGCCCGAAGG
GCCTGGCGGCGATGCCGCAGTTGCGACGGCCGGCCGGCTGGCGTGCGGCGCAGTTGCGGCCGCTTCTCGATGTATCGCGC
CGCGCGATCCGGGCGCATGCGCAACGGCATGGGCTGGCCTGGGTCGAGGATGAGAGCAATCTCGACCTGCGCTATCGCCG
CAACGCGCTGCGCCGGGACATCATGCCGCGGCTCGCGGCGCACTTTCCCGGCAGCGCGCCGACCCTCGCGCGCGCCGCCG
CGCTACAGGCCGAGGCCGCGGGCCTGCTCGACGATCTCGCCGCGCTCGATGCACAGGGCGCAGTCCGGGGAGACCGGCTC
GACTGTGCCGCACTCGCCGCGCTGTCGGCGCCGCGCGCGCGCAATCTCCTGCGCCACTTCATCGAAGGGCGGGGGTGTCC
GTTGCCGAGCGCGCGCGTCCTCGACGAGGCGCGCCTGCAACTCGTCGATGCGCGTCACGACGCGCGCGTACGTGTCAACC
TCAAGGCGGCCGAGTTGTGGCGTTTTCGGGGTCACGCCTATGTCGTGTCGCCGGCGCCCGCGCCGGCCCCAGCCGTGTAC
TGGCACGGCGAACAGAGCCTGTGGGTGCCGGGCGCCGGCTGCCGGGTCGAGTTCCGCCCGACAACGGGGCACGGGCTCAA
GCGCGGGCTTCTGACTTCCGGCACCGTCACGCTCGACGTGCGCCGGGGCGGCGAGCGCCTGAGGCTTCACCCGGGCGGGC
CGCATCGCAGCCTCAAGAACCTGCTGCAGGAGGCGGCCGTGCCGCCGTGGCAGCGCGCGCGGCTGCCGCTTCTGTACCAG
GACGGGCAGCTCGTGTGGGCGGCCGGGATCGGTTTCGACCCCGCCTTCGGCGCCGCGCCGGGCGAAGCGGGGGTGATGCC
GCATGTCGTCTCGCAAGCTGCCGCGCAAGACAGCGGCGGCTGCGGCGTGCTATCCTAG

Upstream 100 bases:

>100_bases
AAGCAGCCGACGGAGGCCATGCTCGGCGCGCGGTATCAGCGCCTGCGGGCGTATGGCAGCTTCAAGGAAGCCCCTGCCCG
GTAAGGCGCCCGCCGACGCG

Downstream 100 bases:

>100_bases
CGGGCTGATTTTCATCGTTTTTTGAACGTTTTCACTCATCGTCCGGAGCACACACATGGCTGTTCAGCGCACTTTCTCCA
TCATCAAACCCGACGCGGTC

Product: cell cycle protein MesJ

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MGDALARHVFSGARLVLALSGGLDSVVLLHVLASLRGRHRFALAAVHVHHGLSPHASDWADFCERLCAAHALDLQVERVH
IAGDDPAGTEAAARRERHRVFARLDADFVLTAHHLDDQAETVLLQLLRGAGPKGLAAMPQLRRPAGWRAAQLRPLLDVSR
RAIRAHAQRHGLAWVEDESNLDLRYRRNALRRDIMPRLAAHFPGSAPTLARAAALQAEAAGLLDDLAALDAQGAVRGDRL
DCAALAALSAPRARNLLRHFIEGRGCPLPSARVLDEARLQLVDARHDARVRVNLKAAELWRFRGHAYVVSPAPAPAPAVY
WHGEQSLWVPGAGCRVEFRPTTGHGLKRGLLTSGTVTLDVRRGGERLRLHPGGPHRSLKNLLQEAAVPPWQRARLPLLYQ
DGQLVWAAGIGFDPAFGAAPGEAGVMPHVVSQAAAQDSGGCGVLS

Sequences:

>Translated_445_residues
MGDALARHVFSGARLVLALSGGLDSVVLLHVLASLRGRHRFALAAVHVHHGLSPHASDWADFCERLCAAHALDLQVERVH
IAGDDPAGTEAAARRERHRVFARLDADFVLTAHHLDDQAETVLLQLLRGAGPKGLAAMPQLRRPAGWRAAQLRPLLDVSR
RAIRAHAQRHGLAWVEDESNLDLRYRRNALRRDIMPRLAAHFPGSAPTLARAAALQAEAAGLLDDLAALDAQGAVRGDRL
DCAALAALSAPRARNLLRHFIEGRGCPLPSARVLDEARLQLVDARHDARVRVNLKAAELWRFRGHAYVVSPAPAPAPAVY
WHGEQSLWVPGAGCRVEFRPTTGHGLKRGLLTSGTVTLDVRRGGERLRLHPGGPHRSLKNLLQEAAVPPWQRARLPLLYQ
DGQLVWAAGIGFDPAFGAAPGEAGVMPHVVSQAAAQDSGGCGVLS
>Mature_444_residues
GDALARHVFSGARLVLALSGGLDSVVLLHVLASLRGRHRFALAAVHVHHGLSPHASDWADFCERLCAAHALDLQVERVHI
AGDDPAGTEAAARRERHRVFARLDADFVLTAHHLDDQAETVLLQLLRGAGPKGLAAMPQLRRPAGWRAAQLRPLLDVSRR
AIRAHAQRHGLAWVEDESNLDLRYRRNALRRDIMPRLAAHFPGSAPTLARAAALQAEAAGLLDDLAALDAQGAVRGDRLD
CAALAALSAPRARNLLRHFIEGRGCPLPSARVLDEARLQLVDARHDARVRVNLKAAELWRFRGHAYVVSPAPAPAPAVYW
HGEQSLWVPGAGCRVEFRPTTGHGLKRGLLTSGTVTLDVRRGGERLRLHPGGPHRSLKNLLQEAAVPPWQRARLPLLYQD
GQLVWAAGIGFDPAFGAAPGEAGVMPHVVSQAAAQDSGGCGVLS

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=443, Percent_Identity=35.8916478555305, Blast_Score=201, Evalue=9e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 47975; Mature: 47843

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDALARHVFSGARLVLALSGGLDSVVLLHVLASLRGRHRFALAAVHVHHGLSPHASDWA
CCHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCHHH
DFCERLCAAHALDLQVERVHIAGDDPAGTEAAARRERHRVFARLDADFVLTAHHLDDQAE
HHHHHHHHHHHHCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHCCCHHH
TVLLQLLRGAGPKGLAAMPQLRRPAGWRAAQLRPLLDVSRRAIRAHAQRHGLAWVEDESN
HHHHHHHHCCCCCCCHHCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
LDLRYRRNALRRDIMPRLAAHFPGSAPTLARAAALQAEAAGLLDDLAALDAQGAVRGDRL
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DCAALAALSAPRARNLLRHFIEGRGCPLPSARVLDEARLQLVDARHDARVRVNLKAAELW
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHH
RFRGHAYVVSPAPAPAPAVYWHGEQSLWVPGAGCRVEFRPTTGHGLKRGLLTSGTVTLDV
HCCCCEEEECCCCCCCCEEEECCCCEEEECCCCCEEEEECCCCCHHHHCCCCCCEEEEEE
RRGGERLRLHPGGPHRSLKNLLQEAAVPPWQRARLPLLYQDGQLVWAAGIGFDPAFGAAP
ECCCCEEEECCCCCHHHHHHHHHHHCCCCHHHCCCCEEEECCCEEEEECCCCCCCCCCCC
GEAGVMPHVVSQAAAQDSGGCGVLS
CCCCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
GDALARHVFSGARLVLALSGGLDSVVLLHVLASLRGRHRFALAAVHVHHGLSPHASDWA
CHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCCCCCHHH
DFCERLCAAHALDLQVERVHIAGDDPAGTEAAARRERHRVFARLDADFVLTAHHLDDQAE
HHHHHHHHHHHHCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHCCCHHH
TVLLQLLRGAGPKGLAAMPQLRRPAGWRAAQLRPLLDVSRRAIRAHAQRHGLAWVEDESN
HHHHHHHHCCCCCCCHHCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCC
LDLRYRRNALRRDIMPRLAAHFPGSAPTLARAAALQAEAAGLLDDLAALDAQGAVRGDRL
CCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
DCAALAALSAPRARNLLRHFIEGRGCPLPSARVLDEARLQLVDARHDARVRVNLKAAELW
HHHHHHHHCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEHHHHH
RFRGHAYVVSPAPAPAPAVYWHGEQSLWVPGAGCRVEFRPTTGHGLKRGLLTSGTVTLDV
HCCCCEEEECCCCCCCCEEEECCCCEEEECCCCCEEEEECCCCCHHHHCCCCCCEEEEEE
RRGGERLRLHPGGPHRSLKNLLQEAAVPPWQRARLPLLYQDGQLVWAAGIGFDPAFGAAP
ECCCCEEEECCCCCHHHHHHHHHHHCCCCHHHCCCCEEEECCCEEEEECCCCCCCCCCCC
GEAGVMPHVVSQAAAQDSGGCGVLS
CCCCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]