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The map label for this gene is greB [H]

Identifier: 74316538

GI number: 74316538

Start: 549958

End: 550521

Strand: Reverse

Name: greB [H]

Synonym: Tbd_0520

Alternate gene names: 74316538

Gene position: 550521-549958 (Counterclockwise)

Preceding gene: 74316539

Following gene: 74316526

Centisome position: 18.92

GC content: 67.91

Gene sequence:

>564_bases
ATGAACAAAGCTTTCGTCAAGGAAACCGACGCCGACGACGCGGACGACGGCGCCGAGATCGCCGCACCGGCCCTGCCGGC
TGGCGCCAAGAACTATATGACGCCGGCCGGCTACGCGCGGCTCGACGCCGAATTCAATCAGCTGTGGAAGGTCGAGCGCC
CCAAGCTCGTCGAAACGATCTCGTGGGCGGCAAGCAACGGCGATCGCTCGGAAAACGGCGACTACATCTATGGCAAGAAG
CGTCTGCGCGAAGTCGACCGCCGCATCCGCTTCCTGTCCAAGCGGCTCGAGCACGCCGAGGTCGTCGACCCGGCGACGCG
CGCGGCGACCGACCAGGTGTTTTTCGGCGCGACCGTCACCGTCGCCGACGCGGACGGCAGCGAATCCACCTACGCGATCG
TCGGCCTCGACGAAGCCGACGCGAGCCGCGGGCGCATCGCCTGGATGTCGCCGATGGCGCGCGCACTGCTGAAGGCGCGC
GAGGGCGACACCGTCGTGGTGCAGACGCCCGACGGGCGTCGCGAGGTCGAGATCATCGAGGTGCGCTACGTCGCGCTGGA
GTAG

Upstream 100 bases:

>100_bases
ACGTGCGCATCGCGCGCGGCCGCCGGACATTCTACTTCCCCTTCACCAGCGTCATTCTCTCGTCGCTGCTGCTCTCCCTG
CTTGCTTACCTACTGGGCCG

Downstream 100 bases:

>100_bases
GGGTCGCGGGTCAGGGGTCAGGGGTCGGGATTCAGGGCCGGTGAGGCCCAGGCCCTGTTGATTTCGTGGCCTCCCGGCCG
CGCATCGCCCGAGCCCTACC

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MNKAFVKETDADDADDGAEIAAPALPAGAKNYMTPAGYARLDAEFNQLWKVERPKLVETISWAASNGDRSENGDYIYGKK
RLREVDRRIRFLSKRLEHAEVVDPATRAATDQVFFGATVTVADADGSESTYAIVGLDEADASRGRIAWMSPMARALLKAR
EGDTVVVQTPDGRREVEIIEVRYVALE

Sequences:

>Translated_187_residues
MNKAFVKETDADDADDGAEIAAPALPAGAKNYMTPAGYARLDAEFNQLWKVERPKLVETISWAASNGDRSENGDYIYGKK
RLREVDRRIRFLSKRLEHAEVVDPATRAATDQVFFGATVTVADADGSESTYAIVGLDEADASRGRIAWMSPMARALLKAR
EGDTVVVQTPDGRREVEIIEVRYVALE
>Mature_187_residues
MNKAFVKETDADDADDGAEIAAPALPAGAKNYMTPAGYARLDAEFNQLWKVERPKLVETISWAASNGDRSENGDYIYGKK
RLREVDRRIRFLSKRLEHAEVVDPATRAATDQVFFGATVTVADADGSESTYAIVGLDEADASRGRIAWMSPMARALLKAR
EGDTVVVQTPDGRREVEIIEVRYVALE

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=152, Percent_Identity=47.3684210526316, Blast_Score=137, Evalue=3e-34,
Organism=Escherichia coli, GI87082225, Length=153, Percent_Identity=38.562091503268, Blast_Score=103, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20609; Mature: 20609

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAFVKETDADDADDGAEIAAPALPAGAKNYMTPAGYARLDAEFNQLWKVERPKLVETI
CCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
SWAASNGDRSENGDYIYGKKRLREVDRRIRFLSKRLEHAEVVDPATRAATDQVFFGATVT
HHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECEEEE
VADADGSESTYAIVGLDEADASRGRIAWMSPMARALLKAREGDTVVVQTPDGRREVEIIE
EEECCCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEE
VRYVALE
EEEEEEC
>Mature Secondary Structure
MNKAFVKETDADDADDGAEIAAPALPAGAKNYMTPAGYARLDAEFNQLWKVERPKLVETI
CCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHH
SWAASNGDRSENGDYIYGKKRLREVDRRIRFLSKRLEHAEVVDPATRAATDQVFFGATVT
HHHCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEECEEEE
VADADGSESTYAIVGLDEADASRGRIAWMSPMARALLKAREGDTVVVQTPDGRREVEIIE
EEECCCCCCEEEEEEECCCCCCCCEEEEHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEE
VRYVALE
EEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]