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The map label for this gene is purB [H]

Identifier: 74316536

GI number: 74316536

Start: 547610

End: 548980

Strand: Direct

Name: purB [H]

Synonym: Tbd_0518

Alternate gene names: 74316536

Gene position: 547610-548980 (Clockwise)

Preceding gene: 74316535

Following gene: 74316537

Centisome position: 18.82

GC content: 65.86

Gene sequence:

>1371_bases
ATGGAACTGACCGCACTGACCGCCCTTTCCCCCCTCGACGGCCGCTACGGCGGCAAGACTGCCGCGCTCCGCGACTTCTT
CAGCGAGTACGCGCTGATCAAGTACCGCGTCATCGTCGAGATCGAATGGCTGAAGGCGCTCGCGGCCGAACCCGCGATCA
CGGAGGTCCCGGCCTTCTCCGACGGTGCGATCGCGCTGCTCGACGCCATCGCCGACCGTTTTTCTGTCGCGGACGCGGAG
CGCGTGAAGGCGATCGAGGCGACGACCAACCACGACGTCAAGGCGGTCGAATATTTCCTCAAGGAAAAAACCCGGGACAA
CGCCGAGATCGGCGCGGTCTCCGAGTTCATCCATTTCGCGTGCACCTCGGAAGACATCAACAACCTGTCGCACGCGCTGA
TGCTCAAGGGCGCGCGCGACGCGGTCCTGCTGCCCGCGCTGGAGAAGCTCATCGCGCGCCTGAGCGACCTCGCCCACGAA
CTCGCCGACCTGCCGATGCTGTCGCGGACCCACGGCCAGCCGGCGTCGCCGACCACGGTCGGCAAGGAGCTCGCCAACGT
CGTCTACCGTCTGCGGCGCGCCTGGGACGGCATCGGCGCGGTCGAGCTGCTCGGCAAGATCAACGGCGCCGTCGGCAATT
ACAACGCCCATCTGTCGGCCTACCCCGACCTCGATTGGGAAGGCTTCGCGAGCGATTTTGTCGGCCATCTCGGGCTCAGC
TTCAATCCCTATACCGTACAGATTGAGCCGCACGACGCGATGGCCGAGCTTTACGACGCGATCGCGCGCACCAACACCGT
ACTCATCGACCTCGACCGCGACGTCTGGGGCTACATCTCGCTCGGCTACTTCAAGCAGAAGACCAAGGCCGGCGAGGTCG
GGTCGTCGACGATGCCGCACAAGGTCAACCCGATCGACTTCGAGAACAGCGAGGGCAACCTCGGTCTTGCCAACGCAGTC
CTGCGCCATCTCGCTGAAAAGCTCCCGGTCTCGCGCTGGCAGCGCGACCTCACCGACTCGACCGTGCTGCGCAACATGGG
GGTCGGCTTCGGCTACAGCCTGCTCGCCTACGAATCCTGCCTGCGCGGCCTTGGCAAACTCGAGGCGAACCCCGCGGCGC
TGGCGACCGACCTCGACGCCAACTGGGAAGTGCTCGCCGAGCCCATCCAGACCGTCATGCGCCGCTACGGCGTCGCCAAC
CCCTACGAGCAGTTGAAGGACCTCACGCGCGGCAAAGGCGGCATGAGCCGCGAGACGCTGCATGCGTTCATCGACGGCTT
GGCGATCCCCGCGGGCGAGAAGGACCGCCTGAAAGCCATGTCACCGGCGTCCTACACGGGCAAGGCGGCGGAGCTGGCGC
GGCAGATCTGA

Upstream 100 bases:

>100_bases
AGGCGGTGCGCGACCGCCGCGCGCTGTAGGCGGCGCCGCACCGCGACGCCCCCGGGCGACGCAGGATAAAAAACGGACAC
ACTCACTTTCGGATGACGCA

Downstream 100 bases:

>100_bases
TTCGCGACAATCGAAACGGAGGCCTCATGTCTGAACATGCTCTCGACGTCGGCCTGGCCGACTTTACCCAATGGGTGCTC
GAGGAGTCGAAGCGCCGCCC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELTALTALSPLDGRYGGKTAALRDFFSEYALIKYRVIVEIEWLKALAAEPAITEVPAFSDGAIALLDAIADRFSVADAE
RVKAIEATTNHDVKAVEYFLKEKTRDNAEIGAVSEFIHFACTSEDINNLSHALMLKGARDAVLLPALEKLIARLSDLAHE
LADLPMLSRTHGQPASPTTVGKELANVVYRLRRAWDGIGAVELLGKINGAVGNYNAHLSAYPDLDWEGFASDFVGHLGLS
FNPYTVQIEPHDAMAELYDAIARTNTVLIDLDRDVWGYISLGYFKQKTKAGEVGSSTMPHKVNPIDFENSEGNLGLANAV
LRHLAEKLPVSRWQRDLTDSTVLRNMGVGFGYSLLAYESCLRGLGKLEANPAALATDLDANWEVLAEPIQTVMRRYGVAN
PYEQLKDLTRGKGGMSRETLHAFIDGLAIPAGEKDRLKAMSPASYTGKAAELARQI

Sequences:

>Translated_456_residues
MELTALTALSPLDGRYGGKTAALRDFFSEYALIKYRVIVEIEWLKALAAEPAITEVPAFSDGAIALLDAIADRFSVADAE
RVKAIEATTNHDVKAVEYFLKEKTRDNAEIGAVSEFIHFACTSEDINNLSHALMLKGARDAVLLPALEKLIARLSDLAHE
LADLPMLSRTHGQPASPTTVGKELANVVYRLRRAWDGIGAVELLGKINGAVGNYNAHLSAYPDLDWEGFASDFVGHLGLS
FNPYTVQIEPHDAMAELYDAIARTNTVLIDLDRDVWGYISLGYFKQKTKAGEVGSSTMPHKVNPIDFENSEGNLGLANAV
LRHLAEKLPVSRWQRDLTDSTVLRNMGVGFGYSLLAYESCLRGLGKLEANPAALATDLDANWEVLAEPIQTVMRRYGVAN
PYEQLKDLTRGKGGMSRETLHAFIDGLAIPAGEKDRLKAMSPASYTGKAAELARQI
>Mature_456_residues
MELTALTALSPLDGRYGGKTAALRDFFSEYALIKYRVIVEIEWLKALAAEPAITEVPAFSDGAIALLDAIADRFSVADAE
RVKAIEATTNHDVKAVEYFLKEKTRDNAEIGAVSEFIHFACTSEDINNLSHALMLKGARDAVLLPALEKLIARLSDLAHE
LADLPMLSRTHGQPASPTTVGKELANVVYRLRRAWDGIGAVELLGKINGAVGNYNAHLSAYPDLDWEGFASDFVGHLGLS
FNPYTVQIEPHDAMAELYDAIARTNTVLIDLDRDVWGYISLGYFKQKTKAGEVGSSTMPHKVNPIDFENSEGNLGLANAV
LRHLAEKLPVSRWQRDLTDSTVLRNMGVGFGYSLLAYESCLRGLGKLEANPAALATDLDANWEVLAEPIQTVMRRYGVAN
PYEQLKDLTRGKGGMSRETLHAFIDGLAIPAGEKDRLKAMSPASYTGKAAELARQI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=67.1052631578947, Blast_Score=647, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323391, Length=352, Percent_Identity=25, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 49789; Mature: 49789

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELTALTALSPLDGRYGGKTAALRDFFSEYALIKYRVIVEIEWLKALAAEPAITEVPAFS
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEHHHHHHHCCCCHHCCCCCC
DGAIALLDAIADRFSVADAERVKAIEATTNHDVKAVEYFLKEKTRDNAEIGAVSEFIHFA
CCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
CTSEDINNLSHALMLKGARDAVLLPALEKLIARLSDLAHELADLPMLSRTHGQPASPTTV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHH
GKELANVVYRLRRAWDGIGAVELLGKINGAVGNYNAHLSAYPDLDWEGFASDFVGHLGLS
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCC
FNPYTVQIEPHDAMAELYDAIARTNTVLIDLDRDVWGYISLGYFKQKTKAGEVGSSTMPH
CCCEEEEECCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
KVNPIDFENSEGNLGLANAVLRHLAEKLPVSRWQRDLTDSTVLRNMGVGFGYSLLAYESC
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LRGLGKLEANPAALATDLDANWEVLAEPIQTVMRRYGVANPYEQLKDLTRGKGGMSRETL
HHHHHCCCCCCHHEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHH
HAFIDGLAIPAGEKDRLKAMSPASYTGKAAELARQI
HHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MELTALTALSPLDGRYGGKTAALRDFFSEYALIKYRVIVEIEWLKALAAEPAITEVPAFS
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEHHHHHHHCCCCHHCCCCCC
DGAIALLDAIADRFSVADAERVKAIEATTNHDVKAVEYFLKEKTRDNAEIGAVSEFIHFA
CCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHH
CTSEDINNLSHALMLKGARDAVLLPALEKLIARLSDLAHELADLPMLSRTHGQPASPTTV
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCHHH
GKELANVVYRLRRAWDGIGAVELLGKINGAVGNYNAHLSAYPDLDWEGFASDFVGHLGLS
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHCCCC
FNPYTVQIEPHDAMAELYDAIARTNTVLIDLDRDVWGYISLGYFKQKTKAGEVGSSTMPH
CCCEEEEECCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
KVNPIDFENSEGNLGLANAVLRHLAEKLPVSRWQRDLTDSTVLRNMGVGFGYSLLAYESC
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
LRGLGKLEANPAALATDLDANWEVLAEPIQTVMRRYGVANPYEQLKDLTRGKGGMSRETL
HHHHHCCCCCCHHEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHH
HAFIDGLAIPAGEKDRLKAMSPASYTGKAAELARQI
HHHHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]