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The map label for this gene is tyrS

Identifier: 74316477

GI number: 74316477

Start: 476495

End: 477685

Strand: Direct

Name: tyrS

Synonym: Tbd_0459

Alternate gene names: 74316477

Gene position: 476495-477685 (Clockwise)

Preceding gene: 74316474

Following gene: 74316478

Centisome position: 16.38

GC content: 62.38

Gene sequence:

>1191_bases
ATGGAGCACGCATTGCAGGAGATCAAGCGTGGCGCGGACGAACTGCTGGTCGAGGCCGAGCTCGTCGAGAAGCTGAAAAC
CGGGCGGCCGTTGCGCATCAAGGCAGGATTCGATCCCACGGCCCCCGATCTCCATCTTGGGCATACGGTCTTATTGAACA
AGTTGCGACAGTTCCAGGTTCTCGGTCACCGGATTGTTTTCCTGATCGGGGATTTCACCGGGATGATCGGCGACCCTACC
GGCAAGAATGCGACCCGCCCGCCGTTGACGCGGGAGGCGGTCGCCGAGAACGCCAAGACCTATCAGGAGCAGGTATTCAA
GGTGCTCGACCCGGCCTTGACCGAGGTGCGCTTCAACTCCGAGTGGATGGAAGGCTTGGGATCGGTCGGGATGATCCGGC
TCGCGGCAACGCATACGGTCGCGAGAATGCTGGAGCGGGACGACTTCCAGAAACGCTACAGCAGCCAGAAGCCGATTGCG
ATCCACGAGTTCCTCTACCCCCTGATCCAGGGTTACGACTCGGTCGAATTGAAGGCCGACGTCGAACTGGGCGGCACCGA
CCAGAAGTTCAACCTGTTGATGGGCCGGGAATTACAGAAGCACTACGGGCAGCCGCCGCAGTGCATTCTGACCATGCCGC
TTCTCGAGGGCACGGACGGCGTCAACAAGATGTCGAAGTCGCTCGGCAATTACATCGGGATCAACGAGGCGCCGGGGCAG
ATTTTCGGCAAGCTCATGTCGATTTCTGACGCGCTGATGTGGCGTTATATCGATCTGCTTTCGTTCGAGTCCATCGCCAG
GATCGAAGGCTGGCGGTCCGACGTCGCGGCCGGCCTGAACCCGCGTGAGGTAAAGGTTGCGTTCGCGCAGGAAATCGTCG
GCAGGTTCCACGGGGACAAGGCCGCACGCGACGCTGTGGCCGAGTTCGAGGCGCGATTCCAGCGTGGGGTCTTGCCAGAC
GATATGCCCGAAGTGGATCTGCACGGCATTGACGGTGGCCTCCCGGTGCCGCAGCTGCTCAAGCAGGCGGGACTCGTTTC
CAGCACCTCGGACGCGATCCGCCAGATCGCCGGCGGCGGGGTCAGGCTCGACGGCGAGCGGGTGACGGACAAAGGCCAGA
GCGTGCCCGCCGGCGCGACGGTCGTCGCGCAGGTCGGCAAGCGGAAATTCGCTCGCGTGACGGTCATTTGA

Upstream 100 bases:

>100_bases
TTGAATTCAAACAGCGGCTTACACACGCGTTTGAGCAAGCGGTGTTGCAGGATTACGGCCAGGATCGAGTGAAACTTGAC
CGGTGCGGGAGAGGAATTTG

Downstream 100 bases:

>100_bases
AAAAAGTTCTGCGATAGCTGTTGACGAAGCTTTTTTGCTCTGTAGAATGCGCACCTCTTCGATGAACAACGCAGTCGTGC
GTCGAAGCAGTAACAAGGAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MEHALQEIKRGADELLVEAELVEKLKTGRPLRIKAGFDPTAPDLHLGHTVLLNKLRQFQVLGHRIVFLIGDFTGMIGDPT
GKNATRPPLTREAVAENAKTYQEQVFKVLDPALTEVRFNSEWMEGLGSVGMIRLAATHTVARMLERDDFQKRYSSQKPIA
IHEFLYPLIQGYDSVELKADVELGGTDQKFNLLMGRELQKHYGQPPQCILTMPLLEGTDGVNKMSKSLGNYIGINEAPGQ
IFGKLMSISDALMWRYIDLLSFESIARIEGWRSDVAAGLNPREVKVAFAQEIVGRFHGDKAARDAVAEFEARFQRGVLPD
DMPEVDLHGIDGGLPVPQLLKQAGLVSSTSDAIRQIAGGGVRLDGERVTDKGQSVPAGATVVAQVGKRKFARVTVI

Sequences:

>Translated_396_residues
MEHALQEIKRGADELLVEAELVEKLKTGRPLRIKAGFDPTAPDLHLGHTVLLNKLRQFQVLGHRIVFLIGDFTGMIGDPT
GKNATRPPLTREAVAENAKTYQEQVFKVLDPALTEVRFNSEWMEGLGSVGMIRLAATHTVARMLERDDFQKRYSSQKPIA
IHEFLYPLIQGYDSVELKADVELGGTDQKFNLLMGRELQKHYGQPPQCILTMPLLEGTDGVNKMSKSLGNYIGINEAPGQ
IFGKLMSISDALMWRYIDLLSFESIARIEGWRSDVAAGLNPREVKVAFAQEIVGRFHGDKAARDAVAEFEARFQRGVLPD
DMPEVDLHGIDGGLPVPQLLKQAGLVSSTSDAIRQIAGGGVRLDGERVTDKGQSVPAGATVVAQVGKRKFARVTVI
>Mature_396_residues
MEHALQEIKRGADELLVEAELVEKLKTGRPLRIKAGFDPTAPDLHLGHTVLLNKLRQFQVLGHRIVFLIGDFTGMIGDPT
GKNATRPPLTREAVAENAKTYQEQVFKVLDPALTEVRFNSEWMEGLGSVGMIRLAATHTVARMLERDDFQKRYSSQKPIA
IHEFLYPLIQGYDSVELKADVELGGTDQKFNLLMGRELQKHYGQPPQCILTMPLLEGTDGVNKMSKSLGNYIGINEAPGQ
IFGKLMSISDALMWRYIDLLSFESIARIEGWRSDVAAGLNPREVKVAFAQEIVGRFHGDKAARDAVAEFEARFQRGVLPD
DMPEVDLHGIDGGLPVPQLLKQAGLVSSTSDAIRQIAGGGVRLDGERVTDKGQSVPAGATVVAQVGKRKFARVTVI

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=416, Percent_Identity=26.2019230769231, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1787925, Length=377, Percent_Identity=29.4429708222812, Blast_Score=140, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25145802, Length=301, Percent_Identity=26.2458471760797, Blast_Score=92, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6325160, Length=329, Percent_Identity=26.4437689969605, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI20130383, Length=402, Percent_Identity=25.1243781094527, Blast_Score=129, Evalue=3e-30,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_THIDA (Q3SLJ8)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314217.1
- ProteinModelPortal:   Q3SLJ8
- SMR:   Q3SLJ8
- STRING:   Q3SLJ8
- GeneID:   3673087
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0459
- NMPDR:   fig|292415.3.peg.1030
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BioCyc:   TDEN292415:TBD_0459-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 43589; Mature: 43589

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 226-226

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHALQEIKRGADELLVEAELVEKLKTGRPLRIKAGFDPTAPDLHLGHTVLLNKLRQFQV
CCHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
LGHRIVFLIGDFTGMIGDPTGKNATRPPLTREAVAENAKTYQEQVFKVLDPALTEVRFNS
HHHHHEEEEECHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EWMEGLGSVGMIRLAATHTVARMLERDDFQKRYSSQKPIAIHEFLYPLIQGYDSVELKAD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEE
VELGGTDQKFNLLMGRELQKHYGQPPQCILTMPLLEGTDGVNKMSKSLGNYIGINEAPGQ
EEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHH
IFGKLMSISDALMWRYIDLLSFESIARIEGWRSDVAAGLNPREVKVAFAQEIVGRFHGDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCH
AARDAVAEFEARFQRGVLPDDMPEVDLHGIDGGLPVPQLLKQAGLVSSTSDAIRQIAGGG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCC
VRLDGERVTDKGQSVPAGATVVAQVGKRKFARVTVI
EEECCCHHCCCCCCCCCCHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MEHALQEIKRGADELLVEAELVEKLKTGRPLRIKAGFDPTAPDLHLGHTVLLNKLRQFQV
CCHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
LGHRIVFLIGDFTGMIGDPTGKNATRPPLTREAVAENAKTYQEQVFKVLDPALTEVRFNS
HHHHHEEEEECHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
EWMEGLGSVGMIRLAATHTVARMLERDDFQKRYSSQKPIAIHEFLYPLIQGYDSVELKAD
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEEEE
VELGGTDQKFNLLMGRELQKHYGQPPQCILTMPLLEGTDGVNKMSKSLGNYIGINEAPGQ
EEECCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCCHH
IFGKLMSISDALMWRYIDLLSFESIARIEGWRSDVAAGLNPREVKVAFAQEIVGRFHGDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCH
AARDAVAEFEARFQRGVLPDDMPEVDLHGIDGGLPVPQLLKQAGLVSSTSDAIRQIAGGG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHCCCC
VRLDGERVTDKGQSVPAGATVVAQVGKRKFARVTVI
EEECCCHHCCCCCCCCCCHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA