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The map label for this gene is bioB

Identifier: 74316337

GI number: 74316337

Start: 342970

End: 343923

Strand: Direct

Name: bioB

Synonym: Tbd_0319

Alternate gene names: 74316337

Gene position: 342970-343923 (Clockwise)

Preceding gene: 74316336

Following gene: 74316338

Centisome position: 11.79

GC content: 67.82

Gene sequence:

>954_bases
ATGAATGACATGACTCCCCCCGTTCCGCGCCGCTCGGTCTCCGAGATCGAAGCGTTGTTTGCCCTGCCCTTCGCCGACCT
GATGTATCAGGCGCAGGGCGTGCACCGTGCACACTTCGACCCAAATCGCATCCAGCTTTCCACGCTGCTGTCGATCAAGA
CCGGGGGCTGCTCCGAGGACTGCGGCTATTGCCCGCAGTCGGTCCACTACGACGCCGGCGTCGAAAGCCAGGGGCTGCTC
GACCTCGGCGACGTGCTCAAGGCCGCCCGCGCGGCGAAGGACGCCGGGGCCTCGCGCTTCTGCATGGGCGCCGCATGGCG
CGGTCCGAAGCAGCGTGAACTGGAACCGGTGCTCGCGATGGTGCGCGAGGTCAAGGCGCTCGGGCTCGAGACCTGCGCCA
CGCTCGGCATGCTCAAGGACGGGCAGGCCGAGCAGCTCAAGGAGGCCGGACTCGACTACTACAACCACAACCTCGACACC
GCACCCGAGTTCTACGGCGAGATCATCACCACCCGCGACTACCAGGACCGCCTCGACACGCTCGAGCGCGTGCGTCGCGC
CGACCTGCACGTGTGCTGCGGCGGCATCGTCGGCATGGGCGAGTCCCGCACCCAGCGCGCGGGCCTCATCGCGCAACTGG
CCGCGCTCGATCCGCAGCCCGAGTCGGTGCCGATCAACCTGCTGGTCCGCGTCGAGGGCACGCCGCTCGCGGAGACCGAG
GCGCTGGAGCCGCTCGAATTCGTGCGTACCATCGCCGTGACCCGGCTTTGCATGCCGAAGAGCTTCGTCCGGCTGTCGGC
GGGTCGGCAGCAGATGAGCGACGCGGTGCAGGCCCTGTGTTTCCTCGCCGGCGCCAATTCGATCTTCTACGGCGAAAAGC
TCCTGACCACGGGCAACCCCGAGTGGGAGCGCGATCAGCGTCTGTTCGACAGCCTGGGCGTGACGGCGTTGTGA

Upstream 100 bases:

>100_bases
GGACCGACGTGACGATCGATGCGGAACCGGGGCCGGGCGGCACGTGAGACTCGATGACCGACAACAACAAGCAATGACGC
GCCAATGACTAGGCAACACG

Downstream 100 bases:

>100_bases
CCGACGGCGCGCTGACCCGCCTGGAACACGGGCTGGCGGCGCTGAAGGCCGATCATCTCGCGCGGCGGCGACCGCTGCTC
GACGGTGCGCAGGGCGCGCA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MNDMTPPVPRRSVSEIEALFALPFADLMYQAQGVHRAHFDPNRIQLSTLLSIKTGGCSEDCGYCPQSVHYDAGVESQGLL
DLGDVLKAARAAKDAGASRFCMGAAWRGPKQRELEPVLAMVREVKALGLETCATLGMLKDGQAEQLKEAGLDYYNHNLDT
APEFYGEIITTRDYQDRLDTLERVRRADLHVCCGGIVGMGESRTQRAGLIAQLAALDPQPESVPINLLVRVEGTPLAETE
ALEPLEFVRTIAVTRLCMPKSFVRLSAGRQQMSDAVQALCFLAGANSIFYGEKLLTTGNPEWERDQRLFDSLGVTAL

Sequences:

>Translated_317_residues
MNDMTPPVPRRSVSEIEALFALPFADLMYQAQGVHRAHFDPNRIQLSTLLSIKTGGCSEDCGYCPQSVHYDAGVESQGLL
DLGDVLKAARAAKDAGASRFCMGAAWRGPKQRELEPVLAMVREVKALGLETCATLGMLKDGQAEQLKEAGLDYYNHNLDT
APEFYGEIITTRDYQDRLDTLERVRRADLHVCCGGIVGMGESRTQRAGLIAQLAALDPQPESVPINLLVRVEGTPLAETE
ALEPLEFVRTIAVTRLCMPKSFVRLSAGRQQMSDAVQALCFLAGANSIFYGEKLLTTGNPEWERDQRLFDSLGVTAL
>Mature_317_residues
MNDMTPPVPRRSVSEIEALFALPFADLMYQAQGVHRAHFDPNRIQLSTLLSIKTGGCSEDCGYCPQSVHYDAGVESQGLL
DLGDVLKAARAAKDAGASRFCMGAAWRGPKQRELEPVLAMVREVKALGLETCATLGMLKDGQAEQLKEAGLDYYNHNLDT
APEFYGEIITTRDYQDRLDTLERVRRADLHVCCGGIVGMGESRTQRAGLIAQLAALDPQPESVPINLLVRVEGTPLAETE
ALEPLEFVRTIAVTRLCMPKSFVRLSAGRQQMSDAVQALCFLAGANSIFYGEKLLTTGNPEWERDQRLFDSLGVTAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=307, Percent_Identity=59.9348534201954, Blast_Score=399, Evalue=1e-112,
Organism=Saccharomyces cerevisiae, GI6321725, Length=316, Percent_Identity=44.620253164557, Blast_Score=301, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_THIDA (Q3SLY0)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314077.1
- ProteinModelPortal:   Q3SLY0
- SMR:   Q3SLY0
- STRING:   Q3SLY0
- GeneID:   3672105
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0319
- NMPDR:   fig|292415.3.peg.551
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q3SLY0
- BioCyc:   TDEN292415:TBD_0319-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 34720; Mature: 34720

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDMTPPVPRRSVSEIEALFALPFADLMYQAQGVHRAHFDPNRIQLSTLLSIKTGGCSED
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEHEEEECCCCCCCC
CGYCPQSVHYDAGVESQGLLDLGDVLKAARAAKDAGASRFCMGAAWRGPKQRELEPVLAM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHH
VREVKALGLETCATLGMLKDGQAEQLKEAGLDYYNHNLDTAPEFYGEIITTRDYQDRLDT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCHHHHHHH
LERVRRADLHVCCGGIVGMGESRTQRAGLIAQLAALDPQPESVPINLLVRVEGTPLAETE
HHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHH
ALEPLEFVRTIAVTRLCMPKSFVRLSAGRQQMSDAVQALCFLAGANSIFYGEKLLTTGNP
HCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEECCCC
EWERDQRLFDSLGVTAL
CHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNDMTPPVPRRSVSEIEALFALPFADLMYQAQGVHRAHFDPNRIQLSTLLSIKTGGCSED
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCEEEEHEEEECCCCCCCC
CGYCPQSVHYDAGVESQGLLDLGDVLKAARAAKDAGASRFCMGAAWRGPKQRELEPVLAM
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHH
VREVKALGLETCATLGMLKDGQAEQLKEAGLDYYNHNLDTAPEFYGEIITTRDYQDRLDT
HHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHCCCCCCCHHHHHHHHCCCCHHHHHHH
LERVRRADLHVCCGGIVGMGESRTQRAGLIAQLAALDPQPESVPINLLVRVEGTPLAETE
HHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHH
ALEPLEFVRTIAVTRLCMPKSFVRLSAGRQQMSDAVQALCFLAGANSIFYGEKLLTTGNP
HCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEECCCC
EWERDQRLFDSLGVTAL
CHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA