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The map label for this gene is asnB [H]

Identifier: 74316310

GI number: 74316310

Start: 310921

End: 312840

Strand: Direct

Name: asnB [H]

Synonym: Tbd_0292

Alternate gene names: 74316310

Gene position: 310921-312840 (Clockwise)

Preceding gene: 74316309

Following gene: 74316311

Centisome position: 10.69

GC content: 66.46

Gene sequence:

>1920_bases
ATGTGCGGCATCACCGGGATTTTCGACACCAGCGGCGTCAACGCGATTTCACGCGACCTGCTGCAGCGCATGAACGAGAC
CCAGCACCACCGCGGTCCCGACGAAGGCGGCCTGCATCTCGAACCTGGACTCGGGCTCGGGCATCGGCGCCTGTCGATCA
TCGACCTGTCGAGCGGTCAGCAGCCCTTGGCGAACGAGGACGGCAGCGTCGTCGTCGTGTTCAACGGCGAGATCTACAAC
TTCCAGGAACTCGTCCCAGAGCTCGAGGCGCTCGGCCATGTCTTCCGCACCCATTCCGACACCGAAGTCATCGTCCACGC
GTGGGAGGCCTGGGGCGAGGCATGCGTCGCGCGCTTTCGCGGCATGTTCGCGTTCGTGCTGTGGGATCGCAACCAGGAAA
CGCTCTTCGTGGTGCGCGACCGCTTCGGCGTCAAACCGCTCTATTACGCCTTCCTCGACACCGGCGAGTTCATTTTCGGT
TCCGAGCTCAAGTCGCTGATGGTCCACCCGAGTCTCGGGCGCGAGGTCGACCCGCTCGCGGTCGAGGACTACTTCGCCTA
CGGCTACGTGCCCGAGCCGCGCACCATCTTCAAGCGCGTGCACAAGCTGCCGCCCGGCTTCACGCTGACGCTGAAGCGCG
GTGCGCCGCGCGCGCTGCCGAAGCAATACTGGGACATGCCCTTCGCGCCGGTCGCGGTGAAGGACGAAGCCGGCGCGATG
GACGAGCTGGTCGAACGCATGAAGGAATCGATCCGCCTGCGCATGATCGCCGACGTGCCGCTCGGCGCCTTCCTCTCGGG
CGGCGTCGACTCGAGCGCCGTCGTCGCGATGATGGCGACCCAGTCGTCGACGCCGGTCAACACCTGCTCGATCGGCTTCG
ACGACCCGGCGTTCAACGAGATCGAGTACGCGCAGCAGGTCGCCGACCGCTACCACACCCGGCACCACGTCGAGACCGTC
GCCGCCGACGACTTCAGCCTGGTTGGCAAGCTCGCCCAGGTCTACGACGAGCCCTACGCTGATTCGTCGGCGCTGCCGAC
CTATCGCGTCTGCGAACTCGCGCGCAAGCACGTCAAGGTCGCGCTGTCGGGCGACGGCGGCGACGAGCATTTCGCCGGCT
ACCGGCGTTACCGCTGGTTCCGCTACGAGGAACGCATGCGCTCCATCATCCCGCTCGGTCTGCGCCGCCCGCTGTTCGGA
ACGCTCGGCCTGCTCTACCCGAAGGCCGACTGGGCACCGAAGATGTTTCGCGCCAAGACGACTTTCGAGGCGCTCGCGCG
CGACACTGTCGAAGGCTATTTCCATGGCGTCTCGCTGCTCTCGAACGCCCAGCGCGCCCAGCTTTTTTCGCCGGCGTTCC
GGCGTGAGCTGCAGGGCTACCAGGCGGTCGACGTGCTGCGACGCCACGCCGCGGTGGCGCCGACCGACGACCCGCTGTCG
CTTGTGCAGTATCTCGACATGAAGACCTATCTCGTCGGCGACATCCTGACCAAGGTCGACCGCGCGAGCATGGCGCATGC
GCTCGAAGTGCGCGAGCCGCTGCTCGATCACGAGCTGATGGGCTGGGTCTCCGGTCTTCCGGTCGACCTCAAGCTGCGCG
GCAGCGAAGGCAAGTACCTGCTCAAGAAGGCGATGGAGCCCTACCTGCCGCACGACGTGTTGTACCGGAAGAAAATGGGC
TTCTCGGTACCGCTCGCGGCCTGGTTCCGCGGCCCGCTCGCGGCGACGATCCGCACGGTCGTGCTCGGCGAGCGGCTCGC
CGGGACCGGCATGTTCAACTGCGAATTCCTCCGCGAGGTGGTCGAGGCGCACCAGTCCGGCCGCCGCGATTTTTCGGTGA
TCCTGTGGACCGTCCTGATGTTCGACGCCTTCCTCGGCCAGGTGCTGGGCCTCGACGACACGGCGCGCGAGGCGGCATGA

Upstream 100 bases:

>100_bases
GGCGCGGGCCTACGCCGACGTTTACGAACAGACGCTTGGCAGGCGACGCCAAACGGCCGTCGACCGGCCCGAATAATCGA
CAGAACAGGATTCGACACGC

Downstream 100 bases:

>100_bases
AGATCCTGCACGTATTCGACCACACGCTGCCGCTGCATTCCGGCTACACCTTCCGCAGCGCGGCGATCCTGCGCAACCAG
CAGAAGCTGGGCTGGCAGAC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 639; Mature: 639

Protein sequence:

>639_residues
MCGITGIFDTSGVNAISRDLLQRMNETQHHRGPDEGGLHLEPGLGLGHRRLSIIDLSSGQQPLANEDGSVVVVFNGEIYN
FQELVPELEALGHVFRTHSDTEVIVHAWEAWGEACVARFRGMFAFVLWDRNQETLFVVRDRFGVKPLYYAFLDTGEFIFG
SELKSLMVHPSLGREVDPLAVEDYFAYGYVPEPRTIFKRVHKLPPGFTLTLKRGAPRALPKQYWDMPFAPVAVKDEAGAM
DELVERMKESIRLRMIADVPLGAFLSGGVDSSAVVAMMATQSSTPVNTCSIGFDDPAFNEIEYAQQVADRYHTRHHVETV
AADDFSLVGKLAQVYDEPYADSSALPTYRVCELARKHVKVALSGDGGDEHFAGYRRYRWFRYEERMRSIIPLGLRRPLFG
TLGLLYPKADWAPKMFRAKTTFEALARDTVEGYFHGVSLLSNAQRAQLFSPAFRRELQGYQAVDVLRRHAAVAPTDDPLS
LVQYLDMKTYLVGDILTKVDRASMAHALEVREPLLDHELMGWVSGLPVDLKLRGSEGKYLLKKAMEPYLPHDVLYRKKMG
FSVPLAAWFRGPLAATIRTVVLGERLAGTGMFNCEFLREVVEAHQSGRRDFSVILWTVLMFDAFLGQVLGLDDTAREAA

Sequences:

>Translated_639_residues
MCGITGIFDTSGVNAISRDLLQRMNETQHHRGPDEGGLHLEPGLGLGHRRLSIIDLSSGQQPLANEDGSVVVVFNGEIYN
FQELVPELEALGHVFRTHSDTEVIVHAWEAWGEACVARFRGMFAFVLWDRNQETLFVVRDRFGVKPLYYAFLDTGEFIFG
SELKSLMVHPSLGREVDPLAVEDYFAYGYVPEPRTIFKRVHKLPPGFTLTLKRGAPRALPKQYWDMPFAPVAVKDEAGAM
DELVERMKESIRLRMIADVPLGAFLSGGVDSSAVVAMMATQSSTPVNTCSIGFDDPAFNEIEYAQQVADRYHTRHHVETV
AADDFSLVGKLAQVYDEPYADSSALPTYRVCELARKHVKVALSGDGGDEHFAGYRRYRWFRYEERMRSIIPLGLRRPLFG
TLGLLYPKADWAPKMFRAKTTFEALARDTVEGYFHGVSLLSNAQRAQLFSPAFRRELQGYQAVDVLRRHAAVAPTDDPLS
LVQYLDMKTYLVGDILTKVDRASMAHALEVREPLLDHELMGWVSGLPVDLKLRGSEGKYLLKKAMEPYLPHDVLYRKKMG
FSVPLAAWFRGPLAATIRTVVLGERLAGTGMFNCEFLREVVEAHQSGRRDFSVILWTVLMFDAFLGQVLGLDDTAREAA
>Mature_639_residues
MCGITGIFDTSGVNAISRDLLQRMNETQHHRGPDEGGLHLEPGLGLGHRRLSIIDLSSGQQPLANEDGSVVVVFNGEIYN
FQELVPELEALGHVFRTHSDTEVIVHAWEAWGEACVARFRGMFAFVLWDRNQETLFVVRDRFGVKPLYYAFLDTGEFIFG
SELKSLMVHPSLGREVDPLAVEDYFAYGYVPEPRTIFKRVHKLPPGFTLTLKRGAPRALPKQYWDMPFAPVAVKDEAGAM
DELVERMKESIRLRMIADVPLGAFLSGGVDSSAVVAMMATQSSTPVNTCSIGFDDPAFNEIEYAQQVADRYHTRHHVETV
AADDFSLVGKLAQVYDEPYADSSALPTYRVCELARKHVKVALSGDGGDEHFAGYRRYRWFRYEERMRSIIPLGLRRPLFG
TLGLLYPKADWAPKMFRAKTTFEALARDTVEGYFHGVSLLSNAQRAQLFSPAFRRELQGYQAVDVLRRHAAVAPTDDPLS
LVQYLDMKTYLVGDILTKVDRASMAHALEVREPLLDHELMGWVSGLPVDLKLRGSEGKYLLKKAMEPYLPHDVLYRKKMG
FSVPLAAWFRGPLAATIRTVVLGERLAGTGMFNCEFLREVVEAHQSGRRDFSVILWTVLMFDAFLGQVLGLDDTAREAA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=418, Percent_Identity=26.555023923445, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI168229252, Length=418, Percent_Identity=26.555023923445, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI168229250, Length=418, Percent_Identity=26.555023923445, Blast_Score=105, Evalue=2e-22,
Organism=Homo sapiens, GI296010848, Length=389, Percent_Identity=26.4781491002571, Blast_Score=99, Evalue=1e-20,
Organism=Escherichia coli, GI1786889, Length=392, Percent_Identity=30.3571428571429, Blast_Score=159, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI71993933, Length=390, Percent_Identity=31.5384615384615, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI25147557, Length=390, Percent_Identity=31.5384615384615, Blast_Score=142, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI25147560, Length=355, Percent_Identity=30.4225352112676, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325403, Length=419, Percent_Identity=27.6849642004773, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6321563, Length=418, Percent_Identity=27.9904306220096, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI45553209, Length=396, Percent_Identity=29.040404040404, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 71746; Mature: 71746

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGIFDTSGVNAISRDLLQRMNETQHHRGPDEGGLHLEPGLGLGHRRLSIIDLSSGQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEECCCCC
QPLANEDGSVVVVFNGEIYNFQELVPELEALGHVFRTHSDTEVIVHAWEAWGEACVARFR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
GMFAFVLWDRNQETLFVVRDRFGVKPLYYAFLDTGEFIFGSELKSLMVHPSLGREVDPLA
HHEEEEEEECCCCEEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCC
VEDYFAYGYVPEPRTIFKRVHKLPPGFTLTLKRGAPRALPKQYWDMPFAPVAVKDEAGAM
HHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCCCCEEECCCCCCH
DELVERMKESIRLRMIADVPLGAFLSGGVDSSAVVAMMATQSSTPVNTCSIGFDDPAFNE
HHHHHHHHHHHEEEEEECCCHHHHHCCCCCCHHHEEEEECCCCCCCCEEECCCCCCCCHH
IEYAQQVADRYHTRHHVETVAADDFSLVGKLAQVYDEPYADSSALPTYRVCELARKHVKV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEE
ALSGDGGDEHFAGYRRYRWFRYEERMRSIIPLGLRRPLFGTLGLLYPKADWAPKMFRAKT
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHCCCCCCCHHHHHHHH
TFEALARDTVEGYFHGVSLLSNAQRAQLFSPAFRRELQGYQAVDVLRRHAAVAPTDDPLS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCCHHH
LVQYLDMKTYLVGDILTKVDRASMAHALEVREPLLDHELMGWVSGLPVDLKLRGSEGKYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
LKKAMEPYLPHDVLYRKKMGFSVPLAAWFRGPLAATIRTVVLGERLAGTGMFNCEFLREV
HHHHCCCCCCHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VEAHQSGRRDFSVILWTVLMFDAFLGQVLGLDDTAREAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure
MCGITGIFDTSGVNAISRDLLQRMNETQHHRGPDEGGLHLEPGLGLGHRRLSIIDLSSGQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCEEEEEECCCCC
QPLANEDGSVVVVFNGEIYNFQELVPELEALGHVFRTHSDTEVIVHAWEAWGEACVARFR
CCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHH
GMFAFVLWDRNQETLFVVRDRFGVKPLYYAFLDTGEFIFGSELKSLMVHPSLGREVDPLA
HHEEEEEEECCCCEEEEEECCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCC
VEDYFAYGYVPEPRTIFKRVHKLPPGFTLTLKRGAPRALPKQYWDMPFAPVAVKDEAGAM
HHHHHHCCCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCCCCCCEEECCCCCCH
DELVERMKESIRLRMIADVPLGAFLSGGVDSSAVVAMMATQSSTPVNTCSIGFDDPAFNE
HHHHHHHHHHHEEEEEECCCHHHHHCCCCCCHHHEEEEECCCCCCCCEEECCCCCCCCHH
IEYAQQVADRYHTRHHVETVAADDFSLVGKLAQVYDEPYADSSALPTYRVCELARKHVKV
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHEEE
ALSGDGGDEHFAGYRRYRWFRYEERMRSIIPLGLRRPLFGTLGLLYPKADWAPKMFRAKT
EEECCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHCCCCCCCHHHHHHHH
TFEALARDTVEGYFHGVSLLSNAQRAQLFSPAFRRELQGYQAVDVLRRHAAVAPTDDPLS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHCCHHHHHHHHHHCCCCCCCCHHH
LVQYLDMKTYLVGDILTKVDRASMAHALEVREPLLDHELMGWVSGLPVDLKLRGSEGKYL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHH
LKKAMEPYLPHDVLYRKKMGFSVPLAAWFRGPLAATIRTVVLGERLAGTGMFNCEFLREV
HHHHCCCCCCHHHHHHHHCCCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VEAHQSGRRDFSVILWTVLMFDAFLGQVLGLDDTAREAA
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]