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The map label for this gene is pyrE

Identifier: 74316273

GI number: 74316273

Start: 276669

End: 277307

Strand: Direct

Name: pyrE

Synonym: Tbd_0255

Alternate gene names: 74316273

Gene position: 276669-277307 (Clockwise)

Preceding gene: 74316269

Following gene: 74316274

Centisome position: 9.51

GC content: 61.97

Gene sequence:

>639_bases
ATGTCCGATTACAGAGCCGAGTTCGTCGAGTTTGCCGTCGCCTCGCAGGTGTTGTGTTTTGGCGAGTTCAAGACCAAGGC
GGGCCGCTTGAGCCCGTATTTTTTCAACGCGGGATTGTTCAACGACGGCGAGAAGCTCAAGCGTCTCGGCGAATTTTACG
CGAAAGCCATCGTCGACTCGGGCATCGCGTTCGACGTGATGTTCGGCCCGGCTTACAAGGGAATTCCGCTCGCCGCGAGC
ATCGTGATCGCACTCGCCGGAATGGGTCGGAACGTGCCGTTCGCGTTCAATCGCAAGGAGGCGAAGGACCACGGCGAAGG
CGGCACGGTGGTCGGTGCGCCCTTGCAGGGGCGCGTGCTGATCGTCGACGACGTGATCTCGGCGGGCACGTCGGTGCGCG
AGTCGGTCGACCTGATCCGAGCGTCGGACGCTACACCCGCCGGCGTCGTGATCGCGCTGGATCGGATGGAACGGGGCCAG
GGCGACAAGTCCGCGGTCCAGGAAGTGCGAGAGCAATACGATATTCCGGTCGTGGCCGTGGTCACGCTGGACAACCTGGT
GGAGTTTCTGGAACGTGATGCGAACCGACGTACGGAATTGCAGGCCGTGGCGAATTACCGCGAAAACTACGGCGTCTAG

Upstream 100 bases:

>100_bases
GCAGCCAGTCGAAGAAGCCCTTGGTGGCCGCCGAACGGATGCCGTTGAGGTTGGCCGATATAATTCGCATCGCGTTTATC
TACTCAAGAAAAGTCCGGAA

Downstream 100 bases:

>100_bases
GCTGCTGGCTGTCGCCCTGGCGCTGGCAGCGGGCGGCGCGCAGGCCGGGATGTACCGCTGGGTCGACGGCAACGGCCGCG
TGCAGTACAGCGACACGCCG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSDYRAEFVEFAVASQVLCFGEFKTKAGRLSPYFFNAGLFNDGEKLKRLGEFYAKAIVDSGIAFDVMFGPAYKGIPLAAS
IVIALAGMGRNVPFAFNRKEAKDHGEGGTVVGAPLQGRVLIVDDVISAGTSVRESVDLIRASDATPAGVVIALDRMERGQ
GDKSAVQEVREQYDIPVVAVVTLDNLVEFLERDANRRTELQAVANYRENYGV

Sequences:

>Translated_212_residues
MSDYRAEFVEFAVASQVLCFGEFKTKAGRLSPYFFNAGLFNDGEKLKRLGEFYAKAIVDSGIAFDVMFGPAYKGIPLAAS
IVIALAGMGRNVPFAFNRKEAKDHGEGGTVVGAPLQGRVLIVDDVISAGTSVRESVDLIRASDATPAGVVIALDRMERGQ
GDKSAVQEVREQYDIPVVAVVTLDNLVEFLERDANRRTELQAVANYRENYGV
>Mature_211_residues
SDYRAEFVEFAVASQVLCFGEFKTKAGRLSPYFFNAGLFNDGEKLKRLGEFYAKAIVDSGIAFDVMFGPAYKGIPLAASI
VIALAGMGRNVPFAFNRKEAKDHGEGGTVVGAPLQGRVLIVDDVISAGTSVRESVDLIRASDATPAGVVIALDRMERGQG
DKSAVQEVREQYDIPVVAVVTLDNLVEFLERDANRRTELQAVANYRENYGV

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=179, Percent_Identity=29.608938547486, Blast_Score=78, Evalue=5e-15,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=57.2769953051643, Blast_Score=258, Evalue=3e-70,
Organism=Caenorhabditis elegans, GI17508631, Length=146, Percent_Identity=31.5068493150685, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323530, Length=220, Percent_Identity=46.8181818181818, Blast_Score=200, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323927, Length=223, Percent_Identity=44.8430493273543, Blast_Score=192, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_THIDA (Q3SM42)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_314013.1
- ProteinModelPortal:   Q3SM42
- SMR:   Q3SM42
- STRING:   Q3SM42
- GeneID:   3671397
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_0255
- NMPDR:   fig|292415.3.peg.362
- eggNOG:   COG0461
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- PhylomeDB:   Q3SM42
- ProtClustDB:   PRK00455
- BioCyc:   TDEN292415:TBD_0255-MONOMER
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23018; Mature: 22887

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 98-98 BINDING 99-99 BINDING 102-102 BINDING 104-104 BINDING 127-127 BINDING 155-155

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDYRAEFVEFAVASQVLCFGEFKTKAGRLSPYFFNAGLFNDGEKLKRLGEFYAKAIVDS
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHC
GIAFDVMFGPAYKGIPLAASIVIALAGMGRNVPFAFNRKEAKDHGEGGTVVGAPLQGRVL
CCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECCCHHHCCCCCCCEEECCCCCCEE
IVDDVISAGTSVRESVDLIRASDATPAGVVIALDRMERGQGDKSAVQEVREQYDIPVVAV
EEECHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECHHCCCCCHHHHHHHHHHHCCCEEEE
VTLDNLVEFLERDANRRTELQAVANYRENYGV
EEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDYRAEFVEFAVASQVLCFGEFKTKAGRLSPYFFNAGLFNDGEKLKRLGEFYAKAIVDS
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHC
GIAFDVMFGPAYKGIPLAASIVIALAGMGRNVPFAFNRKEAKDHGEGGTVVGAPLQGRVL
CCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEECCCHHHCCCCCCCEEECCCCCCEE
IVDDVISAGTSVRESVDLIRASDATPAGVVIALDRMERGQGDKSAVQEVREQYDIPVVAV
EEECHHHCCCHHHHHHHHHHCCCCCCCEEEEEEECHHCCCCCHHHHHHHHHHHCCCEEEE
VTLDNLVEFLERDANRRTELQAVANYRENYGV
EEHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA