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The map label for this gene is sat/cysC [H]

Identifier: 74316228

GI number: 74316228

Start: 229676

End: 231310

Strand: Direct

Name: sat/cysC [H]

Synonym: Tbd_0210

Alternate gene names: 74316228

Gene position: 229676-231310 (Clockwise)

Preceding gene: 74316227

Following gene: 74316232

Centisome position: 7.89

GC content: 66.79

Gene sequence:

>1635_bases
ATGGTGAATCAACTGATCGAGCCCTACGGCGGCACCCTCGTCAATCTGATCGATCCGGAGAAGCGTGAAGCTCTCAAACA
CGAGGCGCTGAGCCTGCCGTCGCTCGACCTCGATTGGCAGCAGCAGTGCGAGCTCGAAATGCTGATGACCGGCGCCTATT
CGCCGCTGACCGGTTTCATGACGCGCGCGCAATGCGCTCGCGTCGAGTCGGCGCAGCAGCTGGACGACGGCAGCTTCTGG
CCGTCTCCGATCACACTGACGAGCCGGGATCGCGCGCTCGCCGACCGTCGGCCGGGCGAGCGCCTCGCGTTGCGTGACGG
CGAAGGCTACATGCTCGCGATCCTGACGCTCAGCGATGTGTGGAAAGACGGCGAGCGCTGGCATCTGGCGGGCGAAGTCG
AGGGCGCGGCCTTGCCTCCACATCCCGACTTCGTCAGCCTGCGCGCGACGCCCGCCGAATTGCGCGCGCTATTCGTTCGC
CGCGGCTGGCGCCGGATCATCGCCTGGCAGGCGCGCCAACCGATGCACCGTGCGCAATACGAGTTCTGTCTGAAGAGCGC
GATCGAGAACGAGGCCAACCTGCTGCTGCATCCGCAGGTCGGCGGCGACATCACGGAGGCGCCAGCGTATTTCGGCCTGG
TGCGCAGCTTTCTCGCGATCCGCGACCGCTTTCCGGCGGCGACCACGCAGCTCTCATTGTTGCCTGCGCCGCCACCCGAG
GCGAGCGGGCGCGCACTGCTGTTGCGCGCGATCGTCGCGCGCAACTTCGGCTGCAGCCTGCTGATCGCGGGCGGCGAGCA
TCAACCCGACGGCGGCGATTGCCGGCGCGGCGAGGACCTCACGCAGAACCGGGTCGATCCCTCGGTCGCCGAACGCGCGG
AAAAAATCGGCGTGCGGCTCATCGCCTACCCACGCATGGTCTACGTCGAGGATCGCGCCGAGCATCTTCCGGAAGCCGAA
GCCCCGCAGGGCGCACGCTTGCTCACGCTTTCCGGCGAGGAATTCCAGCGCCGCATGCGCGCCGGCCTCAAGATCCCCGA
GTGGTACAGCTTTCCCGAAGTGCTGGCCGAACTGCACCGGCAGACGCCGCCCCGCGAGCGCCAGGGGTTCACGGTGTTTT
TCACCGGCCTGTCGGGCGCCGGGAAATCGACGCTCGCCCGCGCGCTGGCCGCGCGGCTCATGGAAATGGGCGGGCGCTGC
GTGACCTTGCTCGACGGCGATATCGTGCGCCGGCATCTGTCGTCGGAGCTGGGCTTTTCGAAAGCGCACCGTGACGTCAA
CGTGCGCCGGATCGGTTTTGTCGCGAGCGAGATCACCAAGAACCGCGGTATCGCGATCTGTGCGCCCATCGCGCCTTACC
GGCAGACGCGCCGTGACGTGCGGGCGATGATCGAGGCGGTCGGCGGCTTCGTCGAAATTCATGTCGCGACGCCGATCGAA
ACCTGCGAGTCGCGCGATCGCAAAGGTCTCTACGCCAAGGCGCGTGCCGGCCTGATCCCGGAATTCACAGGGGTGTCCGA
CCCCTACGAGGTGCCCGAGACGCCCGAACTCGCGATCGATACGACGGGGCTTGCGATCGATGAAGCCGTGCAGCAGATCC
TGCTCAAGCTCGAACACGAAGGCTATCTGCGCTGA

Upstream 100 bases:

>100_bases
AACTCGACCGATTCATTGCTGTTTTTTTCCAGATTTACAACTAGCTCGTGCGCCTCCTCCCCACGAGCGTGCGCCCTATT
CCATCGGACCTTGGAGCAGC

Downstream 100 bases:

>100_bases
ATCGGCGCGAGACGACCCGGTGAAAAGAAGACGGCGACCCGCGGGTCGCCGTCTTCTTTCTGGGCCAGCGTGAGACTTAG
ACGCGGGTCTTGCGGCGGCG

Product: bifunctional sulfate adenylyltransferase subunit 1/adenylylsulfate kinase protein

Products: NA

Alternate protein names: Sulfate adenylyltransferase; ATP-sulfurylase; Sulfate adenylate transferase; SAT; Adenylyl-sulfate kinase; APS kinase; ATP adenosine-5'-phosphosulfate 3'-phosphotransferase; Adenosine-5'-phosphosulfate kinase [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MVNQLIEPYGGTLVNLIDPEKREALKHEALSLPSLDLDWQQQCELEMLMTGAYSPLTGFMTRAQCARVESAQQLDDGSFW
PSPITLTSRDRALADRRPGERLALRDGEGYMLAILTLSDVWKDGERWHLAGEVEGAALPPHPDFVSLRATPAELRALFVR
RGWRRIIAWQARQPMHRAQYEFCLKSAIENEANLLLHPQVGGDITEAPAYFGLVRSFLAIRDRFPAATTQLSLLPAPPPE
ASGRALLLRAIVARNFGCSLLIAGGEHQPDGGDCRRGEDLTQNRVDPSVAERAEKIGVRLIAYPRMVYVEDRAEHLPEAE
APQGARLLTLSGEEFQRRMRAGLKIPEWYSFPEVLAELHRQTPPRERQGFTVFFTGLSGAGKSTLARALAARLMEMGGRC
VTLLDGDIVRRHLSSELGFSKAHRDVNVRRIGFVASEITKNRGIAICAPIAPYRQTRRDVRAMIEAVGGFVEIHVATPIE
TCESRDRKGLYAKARAGLIPEFTGVSDPYEVPETPELAIDTTGLAIDEAVQQILLKLEHEGYLR

Sequences:

>Translated_544_residues
MVNQLIEPYGGTLVNLIDPEKREALKHEALSLPSLDLDWQQQCELEMLMTGAYSPLTGFMTRAQCARVESAQQLDDGSFW
PSPITLTSRDRALADRRPGERLALRDGEGYMLAILTLSDVWKDGERWHLAGEVEGAALPPHPDFVSLRATPAELRALFVR
RGWRRIIAWQARQPMHRAQYEFCLKSAIENEANLLLHPQVGGDITEAPAYFGLVRSFLAIRDRFPAATTQLSLLPAPPPE
ASGRALLLRAIVARNFGCSLLIAGGEHQPDGGDCRRGEDLTQNRVDPSVAERAEKIGVRLIAYPRMVYVEDRAEHLPEAE
APQGARLLTLSGEEFQRRMRAGLKIPEWYSFPEVLAELHRQTPPRERQGFTVFFTGLSGAGKSTLARALAARLMEMGGRC
VTLLDGDIVRRHLSSELGFSKAHRDVNVRRIGFVASEITKNRGIAICAPIAPYRQTRRDVRAMIEAVGGFVEIHVATPIE
TCESRDRKGLYAKARAGLIPEFTGVSDPYEVPETPELAIDTTGLAIDEAVQQILLKLEHEGYLR
>Mature_544_residues
MVNQLIEPYGGTLVNLIDPEKREALKHEALSLPSLDLDWQQQCELEMLMTGAYSPLTGFMTRAQCARVESAQQLDDGSFW
PSPITLTSRDRALADRRPGERLALRDGEGYMLAILTLSDVWKDGERWHLAGEVEGAALPPHPDFVSLRATPAELRALFVR
RGWRRIIAWQARQPMHRAQYEFCLKSAIENEANLLLHPQVGGDITEAPAYFGLVRSFLAIRDRFPAATTQLSLLPAPPPE
ASGRALLLRAIVARNFGCSLLIAGGEHQPDGGDCRRGEDLTQNRVDPSVAERAEKIGVRLIAYPRMVYVEDRAEHLPEAE
APQGARLLTLSGEEFQRRMRAGLKIPEWYSFPEVLAELHRQTPPRERQGFTVFFTGLSGAGKSTLARALAARLMEMGGRC
VTLLDGDIVRRHLSSELGFSKAHRDVNVRRIGFVASEITKNRGIAICAPIAPYRQTRRDVRAMIEAVGGFVEIHVATPIE
TCESRDRKGLYAKARAGLIPEFTGVSDPYEVPETPELAIDTTGLAIDEAVQQILLKLEHEGYLR

Specific function: Catalyzes The Synthesis Of Activated Sulfate. [C]

COG id: COG2046

COG function: function code P; ATP sulfurylase (sulfate adenylyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the APS kinase family [H]

Homologues:

Organism=Homo sapiens, GI46094058, Length=168, Percent_Identity=45.2380952380952, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI34447231, Length=177, Percent_Identity=41.8079096045198, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI62912492, Length=177, Percent_Identity=41.8079096045198, Blast_Score=130, Evalue=2e-30,
Organism=Escherichia coli, GI1789107, Length=199, Percent_Identity=42.2110552763819, Blast_Score=137, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17542422, Length=177, Percent_Identity=43.5028248587571, Blast_Score=141, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6322469, Length=426, Percent_Identity=32.1596244131455, Blast_Score=217, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6322852, Length=181, Percent_Identity=41.9889502762431, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24667032, Length=177, Percent_Identity=42.9378531073446, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24667028, Length=177, Percent_Identity=42.9378531073446, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24667036, Length=177, Percent_Identity=42.9378531073446, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI24667040, Length=177, Percent_Identity=42.9378531073446, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI116007838, Length=177, Percent_Identity=42.9378531073446, Blast_Score=143, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24667044, Length=177, Percent_Identity=42.9378531073446, Blast_Score=143, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002891
- InterPro:   IPR015947
- InterPro:   IPR014729
- InterPro:   IPR020792
- InterPro:   IPR002650 [H]

Pfam domain/function: PF01583 APS_kinase; PF01747 ATP-sulfurylase [H]

EC number: =2.7.7.4; =2.7.1.25 [H]

Molecular weight: Translated: 60489; Mature: 60489

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNQLIEPYGGTLVNLIDPEKREALKHEALSLPSLDLDWQQQCELEMLMTGAYSPLTGFM
CCCCHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHCCCCHHHHHH
TRAQCARVESAQQLDDGSFWPSPITLTSRDRALADRRPGERLALRDGEGYMLAILTLSDV
HHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHCCCCCEEEEECCCCEEEEEEEHHHH
WKDGERWHLAGEVEGAALPPHPDFVSLRATPAELRALFVRRGWRRIIAWQARQPMHRAQY
HCCCCEEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
EFCLKSAIENEANLLLHPQVGGDITEAPAYFGLVRSFLAIRDRFPAATTQLSLLPAPPPE
HHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
ASGRALLLRAIVARNFGCSLLIAGGEHQPDGGDCRRGEDLTQNRVDPSVAERAEKIGVRL
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHCEEE
IAYPRMVYVEDRAEHLPEAEAPQGARLLTLSGEEFQRRMRAGLKIPEWYSFPEVLAELHR
EECCEEEEEECHHHHCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QTPPRERQGFTVFFTGLSGAGKSTLARALAARLMEMGGRCVTLLDGDIVRRHLSSELGFS
CCCCCHHCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCHH
KAHRDVNVRRIGFVASEITKNRGIAICAPIAPYRQTRRDVRAMIEAVGGFVEIHVATPIE
HHHCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCHH
TCESRDRKGLYAKARAGLIPEFTGVSDPYEVPETPELAIDTTGLAIDEAVQQILLKLEHE
HHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCC
GYLR
CCCC
>Mature Secondary Structure
MVNQLIEPYGGTLVNLIDPEKREALKHEALSLPSLDLDWQQQCELEMLMTGAYSPLTGFM
CCCCHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHCCHHHHHHCCCCHHHHHH
TRAQCARVESAQQLDDGSFWPSPITLTSRDRALADRRPGERLALRDGEGYMLAILTLSDV
HHHHHHHHHHHHHCCCCCCCCCCEEECCCCHHHHHCCCCCEEEEECCCCEEEEEEEHHHH
WKDGERWHLAGEVEGAALPPHPDFVSLRATPAELRALFVRRGWRRIIAWQARQPMHRAQY
HCCCCEEEEEECCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
EFCLKSAIENEANLLLHPQVGGDITEAPAYFGLVRSFLAIRDRFPAATTQLSLLPAPPPE
HHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
ASGRALLLRAIVARNFGCSLLIAGGEHQPDGGDCRRGEDLTQNRVDPSVAERAEKIGVRL
CCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHCEEE
IAYPRMVYVEDRAEHLPEAEAPQGARLLTLSGEEFQRRMRAGLKIPEWYSFPEVLAELHR
EECCEEEEEECHHHHCCCCCCCCCCEEEEECCHHHHHHHHCCCCCCCCCCHHHHHHHHHH
QTPPRERQGFTVFFTGLSGAGKSTLARALAARLMEMGGRCVTLLDGDIVRRHLSSELGFS
CCCCCHHCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCHH
KAHRDVNVRRIGFVASEITKNRGIAICAPIAPYRQTRRDVRAMIEAVGGFVEIHVATPIE
HHHCCCCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEEEEEEECCHH
TCESRDRKGLYAKARAGLIPEFTGVSDPYEVPETPELAIDTTGLAIDEAVQQILLKLEHE
HHHCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCC
GYLR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]