Click here to switch to the map view.

The map label for this gene is wcaJ [H]

Identifier: 74316217

GI number: 74316217

Start: 215666

End: 217060

Strand: Reverse

Name: wcaJ [H]

Synonym: Tbd_0199

Alternate gene names: 74316217

Gene position: 217060-215666 (Counterclockwise)

Preceding gene: 74316218

Following gene: 74316216

Centisome position: 7.46

GC content: 65.23

Gene sequence:

>1395_bases
GTGATCCGATTTTTCAGGCACTACGTTCCGCTGAATTTGCTGCTGCTGGTCGCGCTCGAGGCGCTGATCCTCGGCGGCGC
GATCTACGTCGGCGTCAGCGCGCGCTTTCTCGACGCCGGCGCGGTGCCCGAGGATCTGCAGCCGCTCATGCCCAAGGCGC
TCACCTTCGCCGGCGTCATGCTGGCGCTGATGACCGCGAGCGGGCTGTACGATCTCGAGTGGCAAGGCGGCGTCCGCACC
CTGCTCCATCGTCTCGGCCTGAGCTTCGGCCTCGGGCTCGTGACGATGAGCCTGCTGTTCTATTTCGTGCCCGTCCTGCT
CGTCGGGCGTGGCGCCTTCCTGCTTTCCTTCGGCATCGCGCTTCTCGGCATCCTGCTCAGCCGTGCGCTTTTCCTGCGCT
GGGCGCGCGTCGGCGCACTCAAGACGCGCACGCTCGTCATCGGCACGGGCAGCCGCGCGGCCCATATCGAGGCCTTGCTC
ACACGGCGCGGCCACGCCAGCAACGTTCACGTGGTCGGCTATCTGCCCGTCGGCGGCAGCCACCATTTCGTCGACCATGC
GCGCATTCTCGACAGCGACGAGTCGCTGCCACTGCTCGCGCGACGCCTGCACATCGGCGAGATCGTTCTGGCGGTCCGCG
ACCGCCGCGGCGGCGGCATGCCCGTGCAGGACCTCCTCGCCTGCAAACTGCTCGGCATCCGCGTCCTCGAACTGTCGAGC
TTCTTCGAGCGCGAAAACGGCCACCTGCAGCTCGACTCGCTGAACGCGAGCTGGATGATCCTGTCGGAGGGGTTTCATCA
AGGCATGCTCCGGGACACCGCGAAGCGCATGTTCGACCTCCTGGTCAGCAGCGCGATGCTCGCGGTGTGCCTGCCGATCA
TGGCGCTGACCGCGCTCCTCATCAAGCTCGAAAGCCCGGGGCCGGTCCTGTATCGGCAGGAACGTGTCGGTCAGGGCGGA
CGCAACTTCACCATCCTCAAATTCCGCAGCATGTGCGTCGACGCCGAGAAGGACGGGCAGCCGCGCTGGGCGGGGCAGAA
CGACGATCGCGTGACCTGTACCGGGCGCTTCATCCGGCGCACCCGCATTGATGAACTGCCGCAGATATTCAACGTCTTTT
TCGGCGAGATGAGCTTCGTGGGGCCCCGCCCAGAGCGCCCGTATTTCGTGCAGGATCTCACGCAGAAAATCCAGTATTAC
GGCGTGCGCCACATCGTCAAGCCGGGCATCACGGGGTGGGCGCAGGTGCGTTATCCCTACGGCGCGTCCGACGAGGACGC
GATGCACAAACTGCAGTACGACCTCTACTACGTCAAGAACCACAGCCTGTTTCTCGACCTCATGATCCTCTCCCAGACGG
CGCAGGTCGTGCTCTGGGGAAAGGGCGTGCGCTAA

Upstream 100 bases:

>100_bases
AACTACAAAATAATAAGTAGGCATAAGCGACATATAGGGGGAGGAAACTTGGCATGGAAGCTGCTGGCACACCTGCGTCG
ACATTGATCGGCCAGACACT

Downstream 100 bases:

>100_bases
GCGCGCCCAAGTCGCGATGGCCGTCACGCTGCTCCTCCTGTCCGCGCTCGGCCATGGACTCGCGGCGCTCGCCTTCCTGG
CCCTGTCGGGCCTGATCGTC

Product: putative glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MIRFFRHYVPLNLLLLVALEALILGGAIYVGVSARFLDAGAVPEDLQPLMPKALTFAGVMLALMTASGLYDLEWQGGVRT
LLHRLGLSFGLGLVTMSLLFYFVPVLLVGRGAFLLSFGIALLGILLSRALFLRWARVGALKTRTLVIGTGSRAAHIEALL
TRRGHASNVHVVGYLPVGGSHHFVDHARILDSDESLPLLARRLHIGEIVLAVRDRRGGGMPVQDLLACKLLGIRVLELSS
FFERENGHLQLDSLNASWMILSEGFHQGMLRDTAKRMFDLLVSSAMLAVCLPIMALTALLIKLESPGPVLYRQERVGQGG
RNFTILKFRSMCVDAEKDGQPRWAGQNDDRVTCTGRFIRRTRIDELPQIFNVFFGEMSFVGPRPERPYFVQDLTQKIQYY
GVRHIVKPGITGWAQVRYPYGASDEDAMHKLQYDLYYVKNHSLFLDLMILSQTAQVVLWGKGVR

Sequences:

>Translated_464_residues
MIRFFRHYVPLNLLLLVALEALILGGAIYVGVSARFLDAGAVPEDLQPLMPKALTFAGVMLALMTASGLYDLEWQGGVRT
LLHRLGLSFGLGLVTMSLLFYFVPVLLVGRGAFLLSFGIALLGILLSRALFLRWARVGALKTRTLVIGTGSRAAHIEALL
TRRGHASNVHVVGYLPVGGSHHFVDHARILDSDESLPLLARRLHIGEIVLAVRDRRGGGMPVQDLLACKLLGIRVLELSS
FFERENGHLQLDSLNASWMILSEGFHQGMLRDTAKRMFDLLVSSAMLAVCLPIMALTALLIKLESPGPVLYRQERVGQGG
RNFTILKFRSMCVDAEKDGQPRWAGQNDDRVTCTGRFIRRTRIDELPQIFNVFFGEMSFVGPRPERPYFVQDLTQKIQYY
GVRHIVKPGITGWAQVRYPYGASDEDAMHKLQYDLYYVKNHSLFLDLMILSQTAQVVLWGKGVR
>Mature_464_residues
MIRFFRHYVPLNLLLLVALEALILGGAIYVGVSARFLDAGAVPEDLQPLMPKALTFAGVMLALMTASGLYDLEWQGGVRT
LLHRLGLSFGLGLVTMSLLFYFVPVLLVGRGAFLLSFGIALLGILLSRALFLRWARVGALKTRTLVIGTGSRAAHIEALL
TRRGHASNVHVVGYLPVGGSHHFVDHARILDSDESLPLLARRLHIGEIVLAVRDRRGGGMPVQDLLACKLLGIRVLELSS
FFERENGHLQLDSLNASWMILSEGFHQGMLRDTAKRMFDLLVSSAMLAVCLPIMALTALLIKLESPGPVLYRQERVGQGG
RNFTILKFRSMCVDAEKDGQPRWAGQNDDRVTCTGRFIRRTRIDELPQIFNVFFGEMSFVGPRPERPYFVQDLTQKIQYY
GVRHIVKPGITGWAQVRYPYGASDEDAMHKLQYDLYYVKNHSLFLDLMILSQTAQVVLWGKGVR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=178, Percent_Identity=43.8202247191011, Blast_Score=150, Evalue=2e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 51785; Mature: 51785

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFFRHYVPLNLLLLVALEALILGGAIYVGVSARFLDAGAVPEDLQPLMPKALTFAGVM
CCCHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCEEEECCCCCHHHHHHHHHHHHHHHHH
LALMTASGLYDLEWQGGVRTLLHRLGLSFGLGLVTMSLLFYFVPVLLVGRGAFLLSFGIA
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLGILLSRALFLRWARVGALKTRTLVIGTGSRAAHIEALLTRRGHASNVHVVGYLPVGGS
HHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEECCCC
HHFVDHARILDSDESLPLLARRLHIGEIVLAVRDRRGGGMPVQDLLACKLLGIRVLELSS
CCHHHHHHHCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
FFERENGHLQLDSLNASWMILSEGFHQGMLRDTAKRMFDLLVSSAMLAVCLPIMALTALL
HHHCCCCCEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKLESPGPVLYRQERVGQGGRNFTILKFRSMCVDAEKDGQPRWAGQNDDRVTCTGRFIRR
HEECCCCCCEEEHHHCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHH
TRIDELPQIFNVFFGEMSFVGPRPERPYFVQDLTQKIQYYGVRHIVKPGITGWAQVRYPY
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
GASDEDAMHKLQYDLYYVKNHSLFLDLMILSQTAQVVLWGKGVR
CCCCHHHHHHHHHEEEEEECCCEEEHHHHHHCCCEEEEECCCCC
>Mature Secondary Structure
MIRFFRHYVPLNLLLLVALEALILGGAIYVGVSARFLDAGAVPEDLQPLMPKALTFAGVM
CCCHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCEEEECCCCCHHHHHHHHHHHHHHHHH
LALMTASGLYDLEWQGGVRTLLHRLGLSFGLGLVTMSLLFYFVPVLLVGRGAFLLSFGIA
HHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LLGILLSRALFLRWARVGALKTRTLVIGTGSRAAHIEALLTRRGHASNVHVVGYLPVGGS
HHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHHHHHHHHCCCCCCCEEEEEEEECCCC
HHFVDHARILDSDESLPLLARRLHIGEIVLAVRDRRGGGMPVQDLLACKLLGIRVLELSS
CCHHHHHHHCCCCCCCHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHH
FFERENGHLQLDSLNASWMILSEGFHQGMLRDTAKRMFDLLVSSAMLAVCLPIMALTALL
HHHCCCCCEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IKLESPGPVLYRQERVGQGGRNFTILKFRSMCVDAEKDGQPRWAGQNDDRVTCTGRFIRR
HEECCCCCCEEEHHHCCCCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHH
TRIDELPQIFNVFFGEMSFVGPRPERPYFVQDLTQKIQYYGVRHIVKPGITGWAQVRYPY
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
GASDEDAMHKLQYDLYYVKNHSLFLDLMILSQTAQVVLWGKGVR
CCCCHHHHHHHHHEEEEEECCCEEEHHHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]