Click here to switch to the map view.

The map label for this gene is hemB [H]

Identifier: 74316207

GI number: 74316207

Start: 201632

End: 202642

Strand: Direct

Name: hemB [H]

Synonym: Tbd_0189

Alternate gene names: 74316207

Gene position: 201632-202642 (Clockwise)

Preceding gene: 74316206

Following gene: 74316208

Centisome position: 6.93

GC content: 66.07

Gene sequence:

>1011_bases
ATGCTGCCCTTTGGCCAATTTCCGGGGCGGCGCATGCGCCGCATGCGCCGCATGCGCCGCGACGAATTCTCGCGTCGCTT
GATGCGCGAGAACGTGCTCACCGCGCAGGATCTGATTTATCCGCTGTTCGTTCTCGACGGCGAGAACCGCAGCGAGGCGG
TTGGCTCGATGCCGGGGGTCGAGCGGATGTCGCTCGATCGCCTGCTTCCGGTCGCCGAGGAGTGTGTCGAGCTCGGGATT
CCCGCCCTCGCCCTTTTTCCGGTGATCGACGCCGGGCTCAAGAGTGCCGGTGCCGAAGAGGCCGCCAACCCCGAAGGCCT
CGTTCCGCGTGTGGTCGCCGCGCTCAAGGCTCGCTTTCCGGAACTCGGCGTGATCACGGACGTCGCGCTCGATCCCTACA
CCAGCCACGGCCAGGACGGACTCGTCGACGCGTCGGGCTATGTGATGAACGACGAGACCGTGAGCATGCTCGTGCGGCAG
GCCCTGGTTCACGCGGGCGCCGGGGCGGACGTGGTCGCGCCTTCGGACATGATGGACGGCCGGGTCGCCGCCATCCGGCA
AGCGCTCGAGGGCGCGGGTCATGTTCACACGCGCATCCTCGCCTATGCTGCCAAATACGCGTCGAGCTTTTACGGGCCAT
TCCGCGACGCGGTCGGCTCGGCCGCGAACTTGGGCAAGGGCGACAAGTCGACCTATCAGATGGACCCCGCCAATAGCGAC
GAGGCGCTGTGGGAGGTAGGGATGGATTTGCAGGAGGGCGCGGACATGATCATGGTCAAGCCGGGAATGCCGTACCTCGA
CGTCATCCGGCGGGTCAAGGACGCGTTCGGCGCGCCGACCTATGCTTATCAGGTTAGCGGCGAATATGCGATGTTGAAAG
CGGCCGCGCAGAACGGCTGGCTCGACGAGCGTGCCTGCGTGCTCGAAGCCCTGCTCGCATTCAAGCGCGCCGGGGCCGAT
GGCGTGCTGACGTATTTCGCGCGCGACGCCGCGCGTTGGTTACGAAGCTAA

Upstream 100 bases:

>100_bases
GGAGGAGAAGCGGGAGACGGTCCAGGACCGTCTGCTCTACAGAGCAATGCCGCTGCGAAATAGTTCCACGCGAATTCACG
AATTTTTGGAGATGTCTGTG

Downstream 100 bases:

>100_bases
ATTTCGCTGCGCGGGGGGCCGATAAAAGCACGTGGGACCGCCTTTTCCAACTGACGAATCGGGAGTGGCAAGGTGACGCT
GGATTTGCAAAAACTGATCG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MLPFGQFPGRRMRRMRRMRRDEFSRRLMRENVLTAQDLIYPLFVLDGENRSEAVGSMPGVERMSLDRLLPVAEECVELGI
PALALFPVIDAGLKSAGAEEAANPEGLVPRVVAALKARFPELGVITDVALDPYTSHGQDGLVDASGYVMNDETVSMLVRQ
ALVHAGAGADVVAPSDMMDGRVAAIRQALEGAGHVHTRILAYAAKYASSFYGPFRDAVGSAANLGKGDKSTYQMDPANSD
EALWEVGMDLQEGADMIMVKPGMPYLDVIRRVKDAFGAPTYAYQVSGEYAMLKAAAQNGWLDERACVLEALLAFKRAGAD
GVLTYFARDAARWLRS

Sequences:

>Translated_336_residues
MLPFGQFPGRRMRRMRRMRRDEFSRRLMRENVLTAQDLIYPLFVLDGENRSEAVGSMPGVERMSLDRLLPVAEECVELGI
PALALFPVIDAGLKSAGAEEAANPEGLVPRVVAALKARFPELGVITDVALDPYTSHGQDGLVDASGYVMNDETVSMLVRQ
ALVHAGAGADVVAPSDMMDGRVAAIRQALEGAGHVHTRILAYAAKYASSFYGPFRDAVGSAANLGKGDKSTYQMDPANSD
EALWEVGMDLQEGADMIMVKPGMPYLDVIRRVKDAFGAPTYAYQVSGEYAMLKAAAQNGWLDERACVLEALLAFKRAGAD
GVLTYFARDAARWLRS
>Mature_336_residues
MLPFGQFPGRRMRRMRRMRRDEFSRRLMRENVLTAQDLIYPLFVLDGENRSEAVGSMPGVERMSLDRLLPVAEECVELGI
PALALFPVIDAGLKSAGAEEAANPEGLVPRVVAALKARFPELGVITDVALDPYTSHGQDGLVDASGYVMNDETVSMLVRQ
ALVHAGAGADVVAPSDMMDGRVAAIRQALEGAGHVHTRILAYAAKYASSFYGPFRDAVGSAANLGKGDKSTYQMDPANSD
EALWEVGMDLQEGADMIMVKPGMPYLDVIRRVKDAFGAPTYAYQVSGEYAMLKAAAQNGWLDERACVLEALLAFKRAGAD
GVLTYFARDAARWLRS

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=44.4805194805195, Blast_Score=238, Evalue=9e-63,
Organism=Escherichia coli, GI87081728, Length=320, Percent_Identity=44.0625, Blast_Score=232, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6321398, Length=314, Percent_Identity=39.8089171974522, Blast_Score=206, Evalue=6e-54,
Organism=Drosophila melanogaster, GI21358291, Length=296, Percent_Identity=42.2297297297297, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36524; Mature: 36524

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPFGQFPGRRMRRMRRMRRDEFSRRLMRENVLTAQDLIYPLFVLDGENRSEAVGSMPGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCCH
ERMSLDRLLPVAEECVELGIPALALFPVIDAGLKSAGAEEAANPEGLVPRVVAALKARFP
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHCCC
ELGVITDVALDPYTSHGQDGLVDASGYVMNDETVSMLVRQALVHAGAGADVVAPSDMMDG
CCCCHHHHCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHHCH
RVAAIRQALEGAGHVHTRILAYAAKYASSFYGPFRDAVGSAANLGKGDKSTYQMDPANSD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHCCCCCCCCEEECCCCCH
EALWEVGMDLQEGADMIMVKPGMPYLDVIRRVKDAFGAPTYAYQVSGEYAMLKAAAQNGW
HHHHHHCCCHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCC
LDERACVLEALLAFKRAGADGVLTYFARDAARWLRS
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLPFGQFPGRRMRRMRRMRRDEFSRRLMRENVLTAQDLIYPLFVLDGENRSEAVGSMPGV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHCCCCCH
ERMSLDRLLPVAEECVELGIPALALFPVIDAGLKSAGAEEAANPEGLVPRVVAALKARFP
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHCCCCCCHHHHHHHHHHHCCC
ELGVITDVALDPYTSHGQDGLVDASGYVMNDETVSMLVRQALVHAGAGADVVAPSDMMDG
CCCCHHHHCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHHCH
RVAAIRQALEGAGHVHTRILAYAAKYASSFYGPFRDAVGSAANLGKGDKSTYQMDPANSD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHCCCCCCCCEEECCCCCH
EALWEVGMDLQEGADMIMVKPGMPYLDVIRRVKDAFGAPTYAYQVSGEYAMLKAAAQNGW
HHHHHHCCCHHCCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHCCC
LDERACVLEALLAFKRAGADGVLTYFARDAARWLRS
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9529530; 10984043; 10356331; 12079382 [H]