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The map label for this gene is rsmE [H]

Identifier: 74316173

GI number: 74316173

Start: 167369

End: 168097

Strand: Reverse

Name: rsmE [H]

Synonym: Tbd_0155

Alternate gene names: 74316173

Gene position: 168097-167369 (Counterclockwise)

Preceding gene: 74316183

Following gene: 74316172

Centisome position: 5.78

GC content: 71.74

Gene sequence:

>729_bases
ATGTCCTCCCCGCGCTTCTTCCTCGACCAGACGCTGAGCCTCGGCGCCCGCTTCGGCCTGCCCGCCGGCCCGGCGCGTCA
CGCCGCCCGCGCGCTGCGGCTCGCGGCAGGCGACGCGCTCACGCTGTTCAACGGTCGCGGCGGCGAATACACGGCCAGTG
TCGAGCGCATCACCCGGGACGAGGTTGCGGTCGCGATCACGGGCTTTCGCGAGATCGAACGCGAATCGCGTTTGGCAATC
ACGCTGGCGCAGGGGATATCGAGCGGCGAGCGCATGGACTACACGCTGCAAAAGGCGACGGAACTCGGCGTGACGGCGAT
CCAGCCGATCGCCGCCAAGCGCAGCGTCGTCAAGCTCGCCGGTGAGCGCGCCGAGAAACGCGTCGCGCACTGGCAGGGCG
TCGTCGCGAGCGCCTGCGAGCAGTGCGGGCGCAACCGGCTGCCGTCGATCGCGGCGCCGTTGCCGCTCGCCCAGTGGCTC
GGCACGGGCACCGACGAGCGTCTGCTGTTCCTCTCGCCGCTTGCGCAGGCCCGACTCGCCGATCTCGCCGCGCCGAGCGG
ACGCGACGTCCTGATCGCGGGCCCGGAAGGCGGTTTCGAAGCCGACGAAATCGCGGCGCTACATGCGGCCGGCGCCGTGC
CGATCCGCCTCGGCCCGCGCGTGCTGCGCACCGAAACCGCTGCACTCGCGGCGCTGGCTGCGATGCAGACGCTCTGGGGC
GATTTCTGA

Upstream 100 bases:

>100_bases
CTGAAATCCGGGGCTTTCCAGCCGAAATCGCATACCGGCGTAGTGAGGGAAACCATGAGCGCTCCACAGAGTTACGGGAC
AACCCCGGCATAATAGCAAG

Downstream 100 bases:

>100_bases
AGCCGCCCTCCGCTTCGTCACGGGCTTTTGCGCGCGAGCCCGATCCCGCCGACGGGGCGGGATTTTTCGGTATCCTTCTC
CTCCCTACCCAGCCCTCCGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MSSPRFFLDQTLSLGARFGLPAGPARHAARALRLAAGDALTLFNGRGGEYTASVERITRDEVAVAITGFREIERESRLAI
TLAQGISSGERMDYTLQKATELGVTAIQPIAAKRSVVKLAGERAEKRVAHWQGVVASACEQCGRNRLPSIAAPLPLAQWL
GTGTDERLLFLSPLAQARLADLAAPSGRDVLIAGPEGGFEADEIAALHAAGAVPIRLGPRVLRTETAALAALAAMQTLWG
DF

Sequences:

>Translated_242_residues
MSSPRFFLDQTLSLGARFGLPAGPARHAARALRLAAGDALTLFNGRGGEYTASVERITRDEVAVAITGFREIERESRLAI
TLAQGISSGERMDYTLQKATELGVTAIQPIAAKRSVVKLAGERAEKRVAHWQGVVASACEQCGRNRLPSIAAPLPLAQWL
GTGTDERLLFLSPLAQARLADLAAPSGRDVLIAGPEGGFEADEIAALHAAGAVPIRLGPRVLRTETAALAALAAMQTLWG
DF
>Mature_241_residues
SSPRFFLDQTLSLGARFGLPAGPARHAARALRLAAGDALTLFNGRGGEYTASVERITRDEVAVAITGFREIERESRLAIT
LAQGISSGERMDYTLQKATELGVTAIQPIAAKRSVVKLAGERAEKRVAHWQGVVASACEQCGRNRLPSIAAPLPLAQWLG
TGTDERLLFLSPLAQARLADLAAPSGRDVLIAGPEGGFEADEIAALHAAGAVPIRLGPRVLRTETAALAALAAMQTLWGD
F

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=242, Percent_Identity=40.0826446280992, Blast_Score=177, Evalue=8e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25575; Mature: 25443

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS01072 SLH_DOMAIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPRFFLDQTLSLGARFGLPAGPARHAARALRLAAGDALTLFNGRGGEYTASVERITRD
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
EVAVAITGFREIERESRLAITLAQGISSGERMDYTLQKATELGVTAIQPIAAKRSVVKLA
HHEEEEHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GERAEKRVAHWQGVVASACEQCGRNRLPSIAAPLPLAQWLGTGTDERLLFLSPLAQARLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCEEEEECHHHHHHHH
DLAAPSGRDVLIAGPEGGFEADEIAALHAAGAVPIRLGPRVLRTETAALAALAAMQTLWG
HHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHC
DF
CC
>Mature Secondary Structure 
SSPRFFLDQTLSLGARFGLPAGPARHAARALRLAAGDALTLFNGRGGEYTASVERITRD
CCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
EVAVAITGFREIERESRLAITLAQGISSGERMDYTLQKATELGVTAIQPIAAKRSVVKLA
HHEEEEHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GERAEKRVAHWQGVVASACEQCGRNRLPSIAAPLPLAQWLGTGTDERLLFLSPLAQARLA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCEEEEECHHHHHHHH
DLAAPSGRDVLIAGPEGGFEADEIAALHAAGAVPIRLGPRVLRTETAALAALAAMQTLWG
HHCCCCCCEEEEECCCCCCCHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHC
DF
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]