Click here to switch to the map view.

The map label for this gene is argA [H]

Identifier: 74316172

GI number: 74316172

Start: 165928

End: 167256

Strand: Reverse

Name: argA [H]

Synonym: Tbd_0154

Alternate gene names: 74316172

Gene position: 167256-165928 (Counterclockwise)

Preceding gene: 74316173

Following gene: 74316171

Centisome position: 5.75

GC content: 67.04

Gene sequence:

>1329_bases
TTGAAAGACACGACGAATTTCGTTCACTGGTTTCGCTCCGCCGCGCCCTATATCCACGGCTTCCGCGGCAAGACCTTCGT
CATCGCCTTCGGCGGCGAACTGGTCGCGGAGGGAGGCTTCGTCCAGTTGGCCCACGACGTCAACCTGCTCAACAGCCTCG
GCGTGCGCCTCGTGCTCGTCCACGGCGTGCGGCCGCAGGTCGAGGCGCGGCTGACCGAAAACGGCACCGACATCCGCTAC
GTGAGCGGCCTGCGCGTGACCGACGACGCCGCGCTGGCCTGCGTCAAGGAAGCGGCGGGCACGGTACGCGTCGAGATCGA
GGCGCTGTTGTCGCTCGGCATCGCCAATACCCCGATGGCGGGCGCCGACATCCGCGTCGCCTCGGGCAACTTCGTCACTG
CGCAGCCGCTCGGCGTGCGCGACGGCGTCGACCTGCTGCATACCGGCGAAGTCCGCAAGATCGACGCGGCCGCGATCCGG
CGGCGCCTCGACCAGCACGACATCGTGCTGCTGTCGCCGATCGGCTATTCACCGACCGGCGAGATATTCAACCTGAGCCT
CGAAGACGTCGCCACCCAGGCGGCGGTCGAACTCGCTGCCGACAAGCTGATCTTCCTGATGAACACCGAGGGCGTTCCGG
GCAAGGGCCGCACGATCCACAACGCGCTGACCGTCAACGAAGCCCGCCAGGTGCTCGACAAGGCCGGCCGTGCAAAAGCG
CGCGCGCTGCCCGAGGACGTCACCTACTACCTGCCGTGCGCGGTTACCGCCTGCACCCAGGGCGTGAAGCGCGCGCACCT
CATCAGCCGCCACCGCGACGGCGCACTGCTCTCGGAGCTCTTCACGCGCGACGGCGTCGGCACGCTGATCACGCCCGCGC
CGCTCGAAACGCTGCGTCCGGCGACCATCGACGACGTCGGCGGCATCCTCGGCATCATCGAGCCGCTCGAGCGCGACGGC
ATCCTCGTGCGACGCTCGCGCGAACTGCTCGAAATCGAGATCGAGCGCTTTCTGGTGCTCGAGTCCGACGGCGTCATCGC
CGGCTGCGCGGCGCTCTATCCGTTTCCCGAAGAGCAGGCGGCCGAGCTCGCGTGTCTGGCGGTCGCGGCCGACTTCCGAG
GCCGCGGATTCGGCGAGATGCTGCTCGAGGCCGCCGAGAAGACCGGCAAGAAATCCGGCTTCAGGAAGCTCTTCGTGCTG
ACGACCCGCACCGAGCACTGGTTCGAGGAACGCGGCTTCGTCGACAGCAGTCCCGACCACCTGCCCAAGCGTAAACAGGC
GCTTTACAACTACAACCGCAGATCGAAGGTCCTGCAGAAAAAACTCTAG

Upstream 100 bases:

>100_bases
CTTCGTCACGGGCTTTTGCGCGCGAGCCCGATCCCGCCGACGGGGCGGGATTTTTCGGTATCCTTCTCCTCCCTACCCAG
CCCTCCGCCAGGCCAGTCCC

Downstream 100 bases:

>100_bases
GAAACGGCCTTGCACCGACTCTACCGACGGCACCGAGACCGCCCCGCCCTGCCTGCCGGTCGCGTTGGCTGCGCTGCCAC
GTCGCGCGCCGCGCCCCGGA

Product: N-acetylglutamate synthase

Products: NA

Alternate protein names: N-acetylglutamate synthase; AGS; NAGS [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MKDTTNFVHWFRSAAPYIHGFRGKTFVIAFGGELVAEGGFVQLAHDVNLLNSLGVRLVLVHGVRPQVEARLTENGTDIRY
VSGLRVTDDAALACVKEAAGTVRVEIEALLSLGIANTPMAGADIRVASGNFVTAQPLGVRDGVDLLHTGEVRKIDAAAIR
RRLDQHDIVLLSPIGYSPTGEIFNLSLEDVATQAAVELAADKLIFLMNTEGVPGKGRTIHNALTVNEARQVLDKAGRAKA
RALPEDVTYYLPCAVTACTQGVKRAHLISRHRDGALLSELFTRDGVGTLITPAPLETLRPATIDDVGGILGIIEPLERDG
ILVRRSRELLEIEIERFLVLESDGVIAGCAALYPFPEEQAAELACLAVAADFRGRGFGEMLLEAAEKTGKKSGFRKLFVL
TTRTEHWFEERGFVDSSPDHLPKRKQALYNYNRRSKVLQKKL

Sequences:

>Translated_442_residues
MKDTTNFVHWFRSAAPYIHGFRGKTFVIAFGGELVAEGGFVQLAHDVNLLNSLGVRLVLVHGVRPQVEARLTENGTDIRY
VSGLRVTDDAALACVKEAAGTVRVEIEALLSLGIANTPMAGADIRVASGNFVTAQPLGVRDGVDLLHTGEVRKIDAAAIR
RRLDQHDIVLLSPIGYSPTGEIFNLSLEDVATQAAVELAADKLIFLMNTEGVPGKGRTIHNALTVNEARQVLDKAGRAKA
RALPEDVTYYLPCAVTACTQGVKRAHLISRHRDGALLSELFTRDGVGTLITPAPLETLRPATIDDVGGILGIIEPLERDG
ILVRRSRELLEIEIERFLVLESDGVIAGCAALYPFPEEQAAELACLAVAADFRGRGFGEMLLEAAEKTGKKSGFRKLFVL
TTRTEHWFEERGFVDSSPDHLPKRKQALYNYNRRSKVLQKKL
>Mature_442_residues
MKDTTNFVHWFRSAAPYIHGFRGKTFVIAFGGELVAEGGFVQLAHDVNLLNSLGVRLVLVHGVRPQVEARLTENGTDIRY
VSGLRVTDDAALACVKEAAGTVRVEIEALLSLGIANTPMAGADIRVASGNFVTAQPLGVRDGVDLLHTGEVRKIDAAAIR
RRLDQHDIVLLSPIGYSPTGEIFNLSLEDVATQAAVELAADKLIFLMNTEGVPGKGRTIHNALTVNEARQVLDKAGRAKA
RALPEDVTYYLPCAVTACTQGVKRAHLISRHRDGALLSELFTRDGVGTLITPAPLETLRPATIDDVGGILGIIEPLERDG
ILVRRSRELLEIEIERFLVLESDGVIAGCAALYPFPEEQAAELACLAVAADFRGRGFGEMLLEAAEKTGKKSGFRKLFVL
TTRTEHWFEERGFVDSSPDHLPKRKQALYNYNRRSKVLQKKL

Specific function: Arginine biosynthesis; first step. [C]

COG id: COG0548

COG function: function code E; Acetylglutamate kinase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1789181, Length=444, Percent_Identity=49.7747747747748, Blast_Score=429, Evalue=1e-121,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR001048
- InterPro:   IPR010167 [H]

Pfam domain/function: PF00696 AA_kinase; PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.1 [H]

Molecular weight: Translated: 48218; Mature: 48218

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDTTNFVHWFRSAAPYIHGFRGKTFVIAFGGELVAEGGFVQLAHDVNLLNSLGVRLVLV
CCCCHHHHHHHHHCCCHHCCCCCCEEEEEECCCEEECCCEEEEEHHHHHHHHCCEEEEEE
HGVRPQVEARLTENGTDIRYVSGLRVTDDAALACVKEAAGTVRVEIEALLSLGIANTPMA
ECCCCCEEEEECCCCCEEEEEECCEECCHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCC
GADIRVASGNFVTAQPLGVRDGVDLLHTGEVRKIDAAAIRRRLDQHDIVLLSPIGYSPTG
CCEEEEECCCEEEECCCCCCCCCCCEECCCCEEECHHHHHHHCCCCCEEEEECCCCCCCC
EIFNLSLEDVATQAAVELAADKLIFLMNTEGVPGKGRTIHNALTVNEARQVLDKAGRAKA
CEEEEEHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHH
RALPEDVTYYLPCAVTACTQGVKRAHLISRHRDGALLSELFTRDGVGTLITPAPLETLRP
HCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCHHHCCC
ATIDDVGGILGIIEPLERDGILVRRSRELLEIEIERFLVLESDGVIAGCAALYPFPEEQA
CCHHHHHHHHHHHCHHCCCCEEEECCHHHHHEEEEEEEEEECCCCEEEHHHHCCCCHHHH
AELACLAVAADFRGRGFGEMLLEAAEKTGKKSGFRKLFVLTTRTEHWFEERGFVDSSPDH
HHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHCCCCCCCCCC
LPKRKQALYNYNRRSKVLQKKL
CCHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MKDTTNFVHWFRSAAPYIHGFRGKTFVIAFGGELVAEGGFVQLAHDVNLLNSLGVRLVLV
CCCCHHHHHHHHHCCCHHCCCCCCEEEEEECCCEEECCCEEEEEHHHHHHHHCCEEEEEE
HGVRPQVEARLTENGTDIRYVSGLRVTDDAALACVKEAAGTVRVEIEALLSLGIANTPMA
ECCCCCEEEEECCCCCEEEEEECCEECCHHHHHHHHHCCCEEEEEHHHHHHCCCCCCCCC
GADIRVASGNFVTAQPLGVRDGVDLLHTGEVRKIDAAAIRRRLDQHDIVLLSPIGYSPTG
CCEEEEECCCEEEECCCCCCCCCCCEECCCCEEECHHHHHHHCCCCCEEEEECCCCCCCC
EIFNLSLEDVATQAAVELAADKLIFLMNTEGVPGKGRTIHNALTVNEARQVLDKAGRAKA
CEEEEEHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHCCHHH
RALPEDVTYYLPCAVTACTQGVKRAHLISRHRDGALLSELFTRDGVGTLITPAPLETLRP
HCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCEECCCCHHHCCC
ATIDDVGGILGIIEPLERDGILVRRSRELLEIEIERFLVLESDGVIAGCAALYPFPEEQA
CCHHHHHHHHHHHCHHCCCCEEEECCHHHHHEEEEEEEEEECCCCEEEHHHHCCCCHHHH
AELACLAVAADFRGRGFGEMLLEAAEKTGKKSGFRKLFVLTTRTEHWFEERGFVDSSPDH
HHHHHHHEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECCHHHHHHCCCCCCCCCC
LPKRKQALYNYNRRSKVLQKKL
CCHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]