Click here to switch to the map view.

The map label for this gene is hupB [H]

Identifier: 74316025

GI number: 74316025

Start: 6865

End: 7161

Strand: Reverse

Name: hupB [H]

Synonym: Tbd_0007

Alternate gene names: 74316025

Gene position: 7161-6865 (Counterclockwise)

Preceding gene: 74316026

Following gene: 74316024

Centisome position: 0.25

GC content: 64.65

Gene sequence:

>297_bases
ATGAACAAATCTGAATTGATCGACTTCGCTGCCGCCGAGGCTGACATTAGCAAGGCCGCCGCGGGTCGCGCGCTCGACGC
CATCCTGGCCGCCGTGACGAAAGCCGTTGCCAAGGGCGACTCGGTGACCCTGGTCGGCTTCGGTACCTTCAAATCCTCCA
AGCGCGCAGCGCGCACCGGTCGCAACCCCCAGACCGGCAATGAGATCAAGATCGCCGCGACGACCGTGCCGCGCTTCACC
GCAGGCGCCGGCTTCAAGGCCGCGGTTGCCGGCAAGAAGGCGGCCAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
CCATAAGGCGGTACAGCGGGGCGCGACCTAAACAAGGGTAGTGATGGACGCTGGACCGTCCCAGGCTTCTCGTTTTTCTC
AATCAACCATAGGGATACTC

Downstream 100 bases:

>100_bases
TTCTTCGTACCGCGCCGGGCATCGCCCAGACAAAGACCGAGGGCCTCGAAAGAGGCCCTCGTCTTTTTCCGCATGCTACG
GCGTCGCAGCGGCCCGGGTT

Product: histone-like DNA-binding protein

Products: NA

Alternate protein names: HU-1; NS1 [H]

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MNKSELIDFAAAEADISKAAAGRALDAILAAVTKAVAKGDSVTLVGFGTFKSSKRAARTGRNPQTGNEIKIAATTVPRFT
AGAGFKAAVAGKKAAKKK

Sequences:

>Translated_98_residues
MNKSELIDFAAAEADISKAAAGRALDAILAAVTKAVAKGDSVTLVGFGTFKSSKRAARTGRNPQTGNEIKIAATTVPRFT
AGAGFKAAVAGKKAAKKK
>Mature_98_residues
MNKSELIDFAAAEADISKAAAGRALDAILAAVTKAVAKGDSVTLVGFGTFKSSKRAARTGRNPQTGNEIKIAATTVPRFT
AGAGFKAAVAGKKAAKKK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=69.6629213483146, Blast_Score=117, Evalue=2e-28,
Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=58.4269662921348, Blast_Score=106, Evalue=3e-25,

Paralogues:

None

Copy number: 860 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). 2040 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 9922; Mature: 9922

Theoretical pI: Translated: 11.27; Mature: 11.27

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSELIDFAAAEADISKAAAGRALDAILAAVTKAVAKGDSVTLVGFGTFKSSKRAARTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHCC
RNPQTGNEIKIAATTVPRFTAGAGFKAAVAGKKAAKKK
CCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCCCCCCC
>Mature Secondary Structure
MNKSELIDFAAAEADISKAAAGRALDAILAAVTKAVAKGDSVTLVGFGTFKSSKRAARTG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHCC
RNPQTGNEIKIAATTVPRFTAGAGFKAAVAGKKAAKKK
CCCCCCCEEEEEEECCCCCCCCCCCCHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]