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The map label for this gene is oprM [H]

Identifier: 73543124

GI number: 73543124

Start: 3785065

End: 3786555

Strand: Direct

Name: oprM [H]

Synonym: Reut_A3442

Alternate gene names: 73543124

Gene position: 3785065-3786555 (Clockwise)

Preceding gene: 73543123

Following gene: 73543129

Centisome position: 99.44

GC content: 66.2

Gene sequence:

>1491_bases
ATGACCAAGACACTGACCACACTGCTGCTGGTGGCCGGCGTTCTGACGGGCTGCACGCTGGCGCCGAAGTATGAGCGTCC
GGTGCCGCCGGTGGCCGCTGGTTTTCCGGGTGCGCCGGAAGGCTACGCCACGGCCGAAGTGAAGAGCGGCGAGACGCGTC
GTGCCACCGACATCGGCTGGCGCGAGTTCTTCCGCGACCCGCGCCTGCAAACGCTGATTGCGACTTCGCTCGAAAACAAC
CGCGACCTGCGTCAGGCTGCGCTACGCATTGAAGAGGCGCGTTCGCTGTACCAGATCCAGCGTGCCGACCTGCTGCCGAC
GGTGAATGCAAACGCCGGCTACACCCGGGCCCGCACCTCGGCCGCCCAGACGGCGACCGTGCTGGGGCAGCCTTCGGTGA
GCGACCTGTATTCGGTTGGCCTGGGCATCTCGTCCTACGAACTGGACTTCTTCGGACGCGTGCGCAGCCTGTCGAACGCG
GCACTGGCGCAGTACTTCGCCACCGAGGAGGCGCATCGTTCGGCGTATATCTCGCTCGTGTCGGAAGTGGCCAAGGCCTA
CCTGTCCGAGCGCTCGTTCGCCGAGCAGTACGAGATCGCGCGTGACACGCTGAAGGCGCGCGAGAGCACGTATGCACTGG
CCAAGCAGCGCTTCGACGTCGGCGCCACGTCCGCGCTGGACCTGCGCGACAACGAATCGCTGGTGGCGCAGGCACGCGTC
TCCGCCGCGCAGCTGGCACGCCAGCGCGCGCAGGCGCAGAACGCGCTCGAAGTGCTGGTGGGCAAGCCCATCGGCTCGAT
CGAAAATCTGCCAGAGCCGATGCGGCTGTCGGACGAACGCATCATCAGCGATATCCCCGAAGGCCTGCCTTCGGATCTGC
TGGAGCAGCGCCCCGACATCCGCCAGGCCGAGCAGCTGCTGCTGTCGGCTAATGCGAATATCGGTGCGGCACGTGCGGCG
TTCTTCCCGCGCATTACGCTGACCACCAACATCGGCACCATCAGCCCGACGTTCTCGGGCCTGTTCGACGCTGGCACCAA
GGCTTGGTCGTTCGCGCCGCAGCTCACGCTGCCGATCTTCGACTACGGCCGTAACAAGTCGAACCTGGACCTCGCCAACG
TGCGCAAGAACATCGAGGTGGCCAACTACGAGAAGACCATCCAGACGGCCTTCGCGGAAGTGGCGGACGCGCTGGTGGCA
CGCGGCACGCTGGAAGACCAGGTGGCCGGCCAGGAACAGGTGCGCAATGCGGAAGCGGCGCGTTATGAGCTGGCGCGCCT
GCGGTTCCGTAGTGGTGTGGCCAGCTATCTGGATGAGCTCGATGCACAGCGCCAGCTGTTCACCGCCGAACAGGCGCTGA
TTCAGGCGCGGCAGCTGCGGCTGAACAACTCGATCGATCTCTACCGATCGCTTGGTGGCGGGCTGAAGGAGACCGGCGAG
GTGGCGCAGGCGCCGGCTCCGGCTGCGCCGGCGGGGACGGTTACGCAGTAA

Upstream 100 bases:

>100_bases
CCGGTGTTCTTCGTGGTGGTGCGCAAGCGCTTCAAGGGCAGCGAGCGCCAGCACGAATTCGAACGTGCGCGCCTGTCCGC
AGCGCAGGAGGACAATTGAT

Downstream 100 bases:

>100_bases
GGGTCTACAGGTCGGTTCAGGAACGGCGCACTGTGGTGCGCCGTTTTTTTTTAAGCTTCTTGCCGAAGAACGATACCCGC
CGCACTTCGGCGAAGACCGC

Product: RND efflux system, outer membrane lipoprotein, NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MTKTLTTLLLVAGVLTGCTLAPKYERPVPPVAAGFPGAPEGYATAEVKSGETRRATDIGWREFFRDPRLQTLIATSLENN
RDLRQAALRIEEARSLYQIQRADLLPTVNANAGYTRARTSAAQTATVLGQPSVSDLYSVGLGISSYELDFFGRVRSLSNA
ALAQYFATEEAHRSAYISLVSEVAKAYLSERSFAEQYEIARDTLKARESTYALAKQRFDVGATSALDLRDNESLVAQARV
SAAQLARQRAQAQNALEVLVGKPIGSIENLPEPMRLSDERIISDIPEGLPSDLLEQRPDIRQAEQLLLSANANIGAARAA
FFPRITLTTNIGTISPTFSGLFDAGTKAWSFAPQLTLPIFDYGRNKSNLDLANVRKNIEVANYEKTIQTAFAEVADALVA
RGTLEDQVAGQEQVRNAEAARYELARLRFRSGVASYLDELDAQRQLFTAEQALIQARQLRLNNSIDLYRSLGGGLKETGE
VAQAPAPAAPAGTVTQ

Sequences:

>Translated_496_residues
MTKTLTTLLLVAGVLTGCTLAPKYERPVPPVAAGFPGAPEGYATAEVKSGETRRATDIGWREFFRDPRLQTLIATSLENN
RDLRQAALRIEEARSLYQIQRADLLPTVNANAGYTRARTSAAQTATVLGQPSVSDLYSVGLGISSYELDFFGRVRSLSNA
ALAQYFATEEAHRSAYISLVSEVAKAYLSERSFAEQYEIARDTLKARESTYALAKQRFDVGATSALDLRDNESLVAQARV
SAAQLARQRAQAQNALEVLVGKPIGSIENLPEPMRLSDERIISDIPEGLPSDLLEQRPDIRQAEQLLLSANANIGAARAA
FFPRITLTTNIGTISPTFSGLFDAGTKAWSFAPQLTLPIFDYGRNKSNLDLANVRKNIEVANYEKTIQTAFAEVADALVA
RGTLEDQVAGQEQVRNAEAARYELARLRFRSGVASYLDELDAQRQLFTAEQALIQARQLRLNNSIDLYRSLGGGLKETGE
VAQAPAPAAPAGTVTQ
>Mature_495_residues
TKTLTTLLLVAGVLTGCTLAPKYERPVPPVAAGFPGAPEGYATAEVKSGETRRATDIGWREFFRDPRLQTLIATSLENNR
DLRQAALRIEEARSLYQIQRADLLPTVNANAGYTRARTSAAQTATVLGQPSVSDLYSVGLGISSYELDFFGRVRSLSNAA
LAQYFATEEAHRSAYISLVSEVAKAYLSERSFAEQYEIARDTLKARESTYALAKQRFDVGATSALDLRDNESLVAQARVS
AAQLARQRAQAQNALEVLVGKPIGSIENLPEPMRLSDERIISDIPEGLPSDLLEQRPDIRQAEQLLLSANANIGAARAAF
FPRITLTTNIGTISPTFSGLFDAGTKAWSFAPQLTLPIFDYGRNKSNLDLANVRKNIEVANYEKTIQTAFAEVADALVAR
GTLEDQVAGQEQVRNAEAARYELARLRFRSGVASYLDELDAQRQLFTAEQALIQARQLRLNNSIDLYRSLGGGLKETGEV
AQAPAPAAPAGTVTQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=467, Percent_Identity=40.2569593147752, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI2367348, Length=495, Percent_Identity=25.2525252525253, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53989; Mature: 53858

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTLTTLLLVAGVLTGCTLAPKYERPVPPVAAGFPGAPEGYATAEVKSGETRRATDIGW
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHH
REFFRDPRLQTLIATSLENNRDLRQAALRIEEARSLYQIQRADLLPTVNANAGYTRARTS
HHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
AAQTATVLGQPSVSDLYSVGLGISSYELDFFGRVRSLSNAALAQYFATEEAHRSAYISLV
HHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEVAKAYLSERSFAEQYEIARDTLKARESTYALAKQRFDVGATSALDLRDNESLVAQARV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHH
SAAQLARQRAQAQNALEVLVGKPIGSIENLPEPMRLSDERIISDIPEGLPSDLLEQRPDI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCH
RQAEQLLLSANANIGAARAAFFPRITLTTNIGTISPTFSGLFDAGTKAWSFAPQLTLPIF
HHHHHHHHHCCCCCCHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCHHCCCCCEECCHH
DYGRNKSNLDLANVRKNIEVANYEKTIQTAFAEVADALVARGTLEDQVAGQEQVRNAEAA
HCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHH
RYELARLRFRSGVASYLDELDAQRQLFTAEQALIQARQLRLNNSIDLYRSLGGGLKETGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCH
VAQAPAPAAPAGTVTQ
HHCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKTLTTLLLVAGVLTGCTLAPKYERPVPPVAAGFPGAPEGYATAEVKSGETRRATDIGW
CHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCHHHHHH
REFFRDPRLQTLIATSLENNRDLRQAALRIEEARSLYQIQRADLLPTVNANAGYTRARTS
HHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
AAQTATVLGQPSVSDLYSVGLGISSYELDFFGRVRSLSNAALAQYFATEEAHRSAYISLV
HHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SEVAKAYLSERSFAEQYEIARDTLKARESTYALAKQRFDVGATSALDLRDNESLVAQARV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHH
SAAQLARQRAQAQNALEVLVGKPIGSIENLPEPMRLSDERIISDIPEGLPSDLLEQRPDI
HHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCH
RQAEQLLLSANANIGAARAAFFPRITLTTNIGTISPTFSGLFDAGTKAWSFAPQLTLPIF
HHHHHHHHHCCCCCCHHHHHHCCEEEEEECCCCCCCHHHHHHHCCCCHHCCCCCEECCHH
DYGRNKSNLDLANVRKNIEVANYEKTIQTAFAEVADALVARGTLEDQVAGQEQVRNAEAA
HCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHH
RYELARLRFRSGVASYLDELDAQRQLFTAEQALIQARQLRLNNSIDLYRSLGGGLKETGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHCCH
VAQAPAPAAPAGTVTQ
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]