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The map label for this gene is mnmG [H]

Identifier: 73543039

GI number: 73543039

Start: 3673585

End: 3675525

Strand: Reverse

Name: mnmG [H]

Synonym: Reut_A3357

Alternate gene names: 73543039

Gene position: 3675525-3673585 (Counterclockwise)

Preceding gene: 73543040

Following gene: 73543038

Centisome position: 96.56

GC content: 64.04

Gene sequence:

>1941_bases
ATGCTTTACCCGAAAGAATTCGATGTCATTGTCGTCGGCGGTGGTCACGCCGGCACGGAAGCCGCCCTCGCCGCTGCCCG
GATGGGCTGCCAGACCCTGCTGCTGACCCATAACATCGAGACCCTCGGGCAGATGAGCTGCAATCCGTCGATCGGCGGCA
TTGGCAAGGGGCATCTGGTCAAGGAGGTGGACGCGATGGGCGGCGCCATGGCTGCCGCCACGGACGAGGCGGGCATCCAG
TTCCGTATCCTGAACTCCAGCAAGGGGCCGGCCGTGCGAGCGACGCGTGCCCAGGCTGACCGCGTGCTATACCGCAAGGC
TATCCGTACCCGGTTGGAAAACCAGCCGAACCTGATGCTGTTCCAGCAAGCCGTGGACGATCTGCTGGTGGAAGGGGATC
GCGTTGTCGGCGCCGTCACGCAGGTCGGGATCGCTTTTCGCGCCCGAGCGGTGGTACTCACTGCAGGCACCTTCCTGGAC
GGCAAGATCCACGTCGGCCTGGACAACTACACCGGAGGTCGCGCAGGCGACCCGGCGTCGGTGTCACTGTCGGCTCGCCT
GAAGGAATTGAAGCTGCCTCAGGGCCGCCTGAAGACCGGCACGCCGCCGCGCATCGACGGCCGTACCATCGACTTTTCGG
TGATGGAAGAACAACCTGGCGACCTCGATCCGGTGCCGGTGTTCTCGTTCCTGGGCCGTCCGGAGCAGCACCCGCAACAG
CTGCCTTGCTGGATCAGCCATACCAATAGCCGTACCCACGACATCATCCGGGGCGGCCTCGATCGTTCGCCGATGTACAC
CGGCGTGATTGAAGGGGTAGGGCCACGCTACTGCCCGAGCATCGAGGACAAGATCCATCGCTTTGCCAGCAAGGACAGCC
ATCAGATCTTCCTGGAACCGGAGGGGCTGACCACCAACGAGTTCTACCCGAACGGCATCTCGACGAGTCTGCCTTTCGAC
GTCCAACTTGAACTCGTGCACTCGATCCGCGGGCTGGAGAACGCGCACATCCTGCGCCCGGGCTACGCAATCGAGTACGA
CTACTTCGACCCGCGCGCGCTGAAGGCTTCGCTGGAAAGCAAGGCGATCAGCGGGCTGTTCTTTGCCGGCCAGATCAACG
GCACGACCGGCTATGAAGAAGCGGCGGCACAGGGCCTGCTGGCTGGCATCAATGCTGGCTGCTACGTGCGCGAGCGCGAT
GCCTGGACGCCGCGCCGCGACCAGGCATACCTTGGTGTGCTGGTCGACGATCTCATCACGCGCGGCGTGACCGAGCCGTA
CCGCATGTTCACGAGCCGTGCTGAATTCCGCCTGAGCCTGCGTGAAGACAACGCCGACATGCGCCTGACCGAGGTCGGCC
GCGAGCTTGGTGTGGTGGATGACGTGCGCTGGGATGCTTTCAACCGCAAGCGCGACGCTGTTTCACGTGAAACAGAGCGG
CTGAAGAGCACGTGGGTCAATCCGACCATCCTGCCGGCTGAAGATGCCGTGCCGCTGCTGGGCAAGCCGATCGAGCGCGA
GTACAGCCTGGCTGATCTGCTGCGTCGCCCGGAAGTCGTCTACAACGATCTGATGGCGCTGCAGGGCGGACGTCATGCGC
CGGAAGTTCCGCTGGACGGGGACCCGCTGCTGATGGACCAGATCCGCGAGCAGATCGAGATCGGCGTGAAATATCACGGC
TACATCGCCCGTCAGGCTGCCGAGGTGGACAAGCTAGAAGCCAACGAATCGACGCGACTGCCGGAAGGGCTCGACTACAC
CGAAGTGCGCGGGCTGGGCTTCGAGGTCAGCCAGAAGCTGAACCAGCATCGGCCGGAGACGCTTGGCCAGGCCTCGCGTA
TTTCGGGCGTGACCCCGGCGGCGATCTCGCTGCTGCTGGTTCACCTGAAGAAGAAGGGGCTGGGGCGCACCCGTTCCGGC
AACTCGCAAGAGGCTGCCTGA

Upstream 100 bases:

>100_bases
TTCCACGTGAAACAACGCCACCCCCAAAAATCCCCCAGTCGATTGCCGCTATAATTCGACATTCAGCTTTTTCCCGTCAG
CCTCCCGGAGGAGGTGTCCC

Downstream 100 bases:

>100_bases
GTGGGCGGCCAACGACACACCATGATCGACGACGCGGCACAGCGTCGTCGCCTCGAGGCGGGCCTGGCCGAGATCGGCCT
GGCCTTGCCGCCGGATCGGA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 646; Mature: 646

Protein sequence:

>646_residues
MLYPKEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDAMGGAMAAATDEAGIQ
FRILNSSKGPAVRATRAQADRVLYRKAIRTRLENQPNLMLFQQAVDDLLVEGDRVVGAVTQVGIAFRARAVVLTAGTFLD
GKIHVGLDNYTGGRAGDPASVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVMEEQPGDLDPVPVFSFLGRPEQHPQQ
LPCWISHTNSRTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEPEGLTTNEFYPNGISTSLPFD
VQLELVHSIRGLENAHILRPGYAIEYDYFDPRALKASLESKAISGLFFAGQINGTTGYEEAAAQGLLAGINAGCYVRERD
AWTPRRDQAYLGVLVDDLITRGVTEPYRMFTSRAEFRLSLREDNADMRLTEVGRELGVVDDVRWDAFNRKRDAVSRETER
LKSTWVNPTILPAEDAVPLLGKPIEREYSLADLLRRPEVVYNDLMALQGGRHAPEVPLDGDPLLMDQIREQIEIGVKYHG
YIARQAAEVDKLEANESTRLPEGLDYTEVRGLGFEVSQKLNQHRPETLGQASRISGVTPAAISLLLVHLKKKGLGRTRSG
NSQEAA

Sequences:

>Translated_646_residues
MLYPKEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDAMGGAMAAATDEAGIQ
FRILNSSKGPAVRATRAQADRVLYRKAIRTRLENQPNLMLFQQAVDDLLVEGDRVVGAVTQVGIAFRARAVVLTAGTFLD
GKIHVGLDNYTGGRAGDPASVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVMEEQPGDLDPVPVFSFLGRPEQHPQQ
LPCWISHTNSRTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEPEGLTTNEFYPNGISTSLPFD
VQLELVHSIRGLENAHILRPGYAIEYDYFDPRALKASLESKAISGLFFAGQINGTTGYEEAAAQGLLAGINAGCYVRERD
AWTPRRDQAYLGVLVDDLITRGVTEPYRMFTSRAEFRLSLREDNADMRLTEVGRELGVVDDVRWDAFNRKRDAVSRETER
LKSTWVNPTILPAEDAVPLLGKPIEREYSLADLLRRPEVVYNDLMALQGGRHAPEVPLDGDPLLMDQIREQIEIGVKYHG
YIARQAAEVDKLEANESTRLPEGLDYTEVRGLGFEVSQKLNQHRPETLGQASRISGVTPAAISLLLVHLKKKGLGRTRSG
NSQEAA
>Mature_646_residues
MLYPKEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLVKEVDAMGGAMAAATDEAGIQ
FRILNSSKGPAVRATRAQADRVLYRKAIRTRLENQPNLMLFQQAVDDLLVEGDRVVGAVTQVGIAFRARAVVLTAGTFLD
GKIHVGLDNYTGGRAGDPASVSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVMEEQPGDLDPVPVFSFLGRPEQHPQQ
LPCWISHTNSRTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEPEGLTTNEFYPNGISTSLPFD
VQLELVHSIRGLENAHILRPGYAIEYDYFDPRALKASLESKAISGLFFAGQINGTTGYEEAAAQGLLAGINAGCYVRERD
AWTPRRDQAYLGVLVDDLITRGVTEPYRMFTSRAEFRLSLREDNADMRLTEVGRELGVVDDVRWDAFNRKRDAVSRETER
LKSTWVNPTILPAEDAVPLLGKPIEREYSLADLLRRPEVVYNDLMALQGGRHAPEVPLDGDPLLMDQIREQIEIGVKYHG
YIARQAAEVDKLEANESTRLPEGLDYTEVRGLGFEVSQKLNQHRPETLGQASRISGVTPAAISLLLVHLKKKGLGRTRSG
NSQEAA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=647, Percent_Identity=44.3585780525502, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI19882217, Length=672, Percent_Identity=42.7083333333333, Blast_Score=472, Evalue=1e-133,
Organism=Homo sapiens, GI183227703, Length=687, Percent_Identity=41.7758369723435, Blast_Score=467, Evalue=1e-131,
Organism=Escherichia coli, GI2367273, Length=636, Percent_Identity=70.5974842767296, Blast_Score=913, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=634, Percent_Identity=41.1671924290221, Blast_Score=469, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6321202, Length=633, Percent_Identity=45.1816745655608, Blast_Score=505, Evalue=1e-144,
Organism=Drosophila melanogaster, GI24658174, Length=656, Percent_Identity=45.1219512195122, Blast_Score=504, Evalue=1e-143,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 70916; Mature: 70916

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPKEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHHHCHHEEEEECCHHHHHCCCCCCCCCCCCCCHHH
KEVDAMGGAMAAATDEAGIQFRILNSSKGPAVRATRAQADRVLYRKAIRTRLENQPNLML
HHHHHCCCCEEECCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEH
FQQAVDDLLVEGDRVVGAVTQVGIAFRARAVVLTAGTFLDGKIHVGLDNYTGGRAGDPAS
HHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEECEEECCEEEEEECCCCCCCCCCCCC
VSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVMEEQPGDLDPVPVFSFLGRPEQHPQQ
EEHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCCCCC
LPCWISHTNSRTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEP
CCEEEECCCCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECC
EGLTTNEFYPNGISTSLPFDVQLELVHSIRGLENAHILRPGYAIEYDYFDPRALKASLES
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCEEEEECCCCCHHHHHHHHH
KAISGLFFAGQINGTTGYEEAAAQGLLAGINAGCYVRERDAWTPRRDQAYLGVLVDDLIT
HHHCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
RGVTEPYRMFTSRAEFRLSLREDNADMRLTEVGRELGVVDDVRWDAFNRKRDAVSRETER
HCCCHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LKSTWVNPTILPAEDAVPLLGKPIEREYSLADLLRRPEVVYNDLMALQGGRHAPEVPLDG
HHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
DPLLMDQIREQIEIGVKYHGYIARQAAEVDKLEANESTRLPEGLDYTEVRGLGFEVSQKL
CCHHHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHH
NQHRPETLGQASRISGVTPAAISLLLVHLKKKGLGRTRSGNSQEAA
HHCCCHHHCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLYPKEFDVIVVGGGHAGTEAALAAARMGCQTLLLTHNIETLGQMSCNPSIGGIGKGHLV
CCCCCCCCEEEEECCCCCHHHHHHHHHHCHHEEEEECCHHHHHCCCCCCCCCCCCCCHHH
KEVDAMGGAMAAATDEAGIQFRILNSSKGPAVRATRAQADRVLYRKAIRTRLENQPNLML
HHHHHCCCCEEECCCCCCEEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEH
FQQAVDDLLVEGDRVVGAVTQVGIAFRARAVVLTAGTFLDGKIHVGLDNYTGGRAGDPAS
HHHHHHHHHHCCCHHHHHHHHHHHHHHEEEEEEEECEEECCEEEEEECCCCCCCCCCCCC
VSLSARLKELKLPQGRLKTGTPPRIDGRTIDFSVMEEQPGDLDPVPVFSFLGRPEQHPQQ
EEHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCCCCC
LPCWISHTNSRTHDIIRGGLDRSPMYTGVIEGVGPRYCPSIEDKIHRFASKDSHQIFLEP
CCEEEECCCCCHHHHHHCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECC
EGLTTNEFYPNGISTSLPFDVQLELVHSIRGLENAHILRPGYAIEYDYFDPRALKASLES
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEECCCEEEEECCCCCHHHHHHHHH
KAISGLFFAGQINGTTGYEEAAAQGLLAGINAGCYVRERDAWTPRRDQAYLGVLVDDLIT
HHHCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHH
RGVTEPYRMFTSRAEFRLSLREDNADMRLTEVGRELGVVDDVRWDAFNRKRDAVSRETER
HCCCHHHHHHHCCCCEEEEEECCCCCEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LKSTWVNPTILPAEDAVPLLGKPIEREYSLADLLRRPEVVYNDLMALQGGRHAPEVPLDG
HHHHCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
DPLLMDQIREQIEIGVKYHGYIARQAAEVDKLEANESTRLPEGLDYTEVRGLGFEVSQKL
CCHHHHHHHHHHHCCCEECCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCHHHHHHH
NQHRPETLGQASRISGVTPAAISLLLVHLKKKGLGRTRSGNSQEAA
HHCCCHHHCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA