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The map label for this gene is gidB

Identifier: 73543038

GI number: 73543038

Start: 3672877

End: 3673584

Strand: Reverse

Name: gidB

Synonym: Reut_A3356

Alternate gene names: 73543038

Gene position: 3673584-3672877 (Counterclockwise)

Preceding gene: 73543039

Following gene: 73543037

Centisome position: 96.51

GC content: 65.82

Gene sequence:

>708_bases
GTGGGCGGCCAACGACACACCATGATCGACGACGCGGCACAGCGTCGTCGCCTCGAGGCGGGCCTGGCCGAGATCGGCCT
GGCCTTGCCGCCGGATCGGATCGACACCCTGTTCGCATACCTGGCGCTGCTGCGCAAGTGGAACGGCGTCTATAACCTGA
CTGCGATCCGTCATCCCGATGAAATGCTGACGCACCATCTGCTCGATTCGCTGGCCGCCGTGCCGGCGCTGGCGGAGATG
GCGCGCTCGTCGGAGGTTGGCGGTGCTGCGCGGGGCAGGGTGCTCGATGTAGGCTCGGGCGGCGGCATGCCCGGCCTTCC
GCTGGCGATTTCGTGCCCCGACGTGTCGGTGCTGATGGTCGACATCGTCCAGAAGAAGACCGCGTTCCTGACGCAGTGCC
GGGCGCAGCTCGGCTTGTCGAATGCGGCCGCCCATTGGGGCCCGGTCGAGAGGTTGGAAGATCCAGATAAATTTGCGGTG
ATTACGTCACGTGCGTTTGCGGAACTGACGGACTTCGTCAACCTGTCCGGTCATCTGCTGGCACCGCACGGCAGGCTCAT
CGCGATGAAGGGCGTGTACCCGCAGGCTGAACTGGATCGCATGGAAGCGGCGGGGCTGATGGCGGCGTGGCAGGTCGAGG
AAGTGCGGCGCCTCACTGTCCCGGGGCTCGATGCCGAGCGCCACCTGGTGCTGTTGTCGCGTAAATGA

Upstream 100 bases:

>100_bases
TTCGGGCGTGACCCCGGCGGCGATCTCGCTGCTGCTGGTTCACCTGAAGAAGAAGGGGCTGGGGCGCACCCGTTCCGGCA
ACTCGCAAGAGGCTGCCTGA

Downstream 100 bases:

>100_bases
TGGTAGCGGCGCGTGCGCCGCGAACCGGGCGGGCCAGCGGGCCATGTCCGGATAAGGAATTCAATCGGGAGCCTCAGAAG
CATATGGCCAAGGTATTCGT

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 235; Mature: 234

Protein sequence:

>235_residues
MGGQRHTMIDDAAQRRRLEAGLAEIGLALPPDRIDTLFAYLALLRKWNGVYNLTAIRHPDEMLTHHLLDSLAAVPALAEM
ARSSEVGGAARGRVLDVGSGGGMPGLPLAISCPDVSVLMVDIVQKKTAFLTQCRAQLGLSNAAAHWGPVERLEDPDKFAV
ITSRAFAELTDFVNLSGHLLAPHGRLIAMKGVYPQAELDRMEAAGLMAAWQVEEVRRLTVPGLDAERHLVLLSRK

Sequences:

>Translated_235_residues
MGGQRHTMIDDAAQRRRLEAGLAEIGLALPPDRIDTLFAYLALLRKWNGVYNLTAIRHPDEMLTHHLLDSLAAVPALAEM
ARSSEVGGAARGRVLDVGSGGGMPGLPLAISCPDVSVLMVDIVQKKTAFLTQCRAQLGLSNAAAHWGPVERLEDPDKFAV
ITSRAFAELTDFVNLSGHLLAPHGRLIAMKGVYPQAELDRMEAAGLMAAWQVEEVRRLTVPGLDAERHLVLLSRK
>Mature_234_residues
GGQRHTMIDDAAQRRRLEAGLAEIGLALPPDRIDTLFAYLALLRKWNGVYNLTAIRHPDEMLTHHLLDSLAAVPALAEMA
RSSEVGGAARGRVLDVGSGGGMPGLPLAISCPDVSVLMVDIVQKKTAFLTQCRAQLGLSNAAAHWGPVERLEDPDKFAVI
TSRAFAELTDFVNLSGHLLAPHGRLIAMKGVYPQAELDRMEAAGLMAAWQVEEVRRLTVPGLDAERHLVLLSRK

Specific function: Specifically methylates the N7 position of guanosine in position 527 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=218, Percent_Identity=37.6146788990826, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_CUPPJ (Q46VX0)

Other databases:

- EMBL:   CP000090
- RefSeq:   YP_297558.1
- ProteinModelPortal:   Q46VX0
- SMR:   Q46VX0
- GeneID:   3612061
- GenomeReviews:   CP000090_GR
- KEGG:   reu:Reut_A3356
- NMPDR:   fig|264198.3.peg.3843
- HOGENOM:   HBG686577
- OMA:   HIMDSIV
- ProtClustDB:   PRK00107
- BioCyc:   REUT264198:REUT_A3356-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: =2.1.1.170

Molecular weight: Translated: 25466; Mature: 25335

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGQRHTMIDDAAQRRRLEAGLAEIGLALPPDRIDTLFAYLALLRKWNGVYNLTAIRHPD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCH
EMLTHHLLDSLAAVPALAEMARSSEVGGAARGRVLDVGSGGGMPGLPLAISCPDVSVLMV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEECCCHHHHHH
DIVQKKTAFLTQCRAQLGLSNAAAHWGPVERLEDPDKFAVITSRAFAELTDFVNLSGHLL
HHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHCCCCCCEEEEHHHHHHHHHHHHCCCCCEE
APHGRLIAMKGVYPQAELDRMEAAGLMAAWQVEEVRRLTVPGLDAERHLVLLSRK
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
>Mature Secondary Structure 
GGQRHTMIDDAAQRRRLEAGLAEIGLALPPDRIDTLFAYLALLRKWNGVYNLTAIRHPD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCH
EMLTHHLLDSLAAVPALAEMARSSEVGGAARGRVLDVGSGGGMPGLPLAISCPDVSVLMV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCEEEECCCHHHHHH
DIVQKKTAFLTQCRAQLGLSNAAAHWGPVERLEDPDKFAVITSRAFAELTDFVNLSGHLL
HHHHHHHHHHHHHHHHHCCCCCHHCCCCHHHCCCCCCEEEEHHHHHHHHHHHHCCCCCEE
APHGRLIAMKGVYPQAELDRMEAAGLMAAWQVEEVRRLTVPGLDAERHLVLLSRK
CCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA