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The map label for this gene is rep [H]

Identifier: 73543009

GI number: 73543009

Start: 3635779

End: 3637893

Strand: Reverse

Name: rep [H]

Synonym: Reut_A3327

Alternate gene names: 73543009

Gene position: 3637893-3635779 (Counterclockwise)

Preceding gene: 73543018

Following gene: 73543008

Centisome position: 95.57

GC content: 63.64

Gene sequence:

>2115_bases
ATGACTCACGGCCTCAACGCTGCCCAATCCGAAGCCGTCCGCTACCTGGACGGACCCTGCCTCGTGCTCGCAGGCGCGGG
TTCGGGCAAGACGCGAGTGATCACGCAGAAGATCGCCCACCTGATCGAAGACAAGGGTTTCGAGCCGCGCCACATCGCCG
CTGTCACCTTCACCAACAAGGCAGCCAAGGAGATGCAGGAGCGCATCGGCAAGCTGATGGAAGGCAAGACCACGCGCGAG
GGCAAGCGCCTGCCGCTCAAGCAGCTCACGGTCTGTACCTTCCACTCGCTGGGCGTGCAGATCCTGCGCATGGAAGCCGA
GCACGTGGGCCTCAAGCCGCAGTTCTCGATCATGGATTCGGACGACTGCTTCGGCCTGATCCAGGAGCAGCTGGGCACCA
CCGACAAGAAGCTGATCCGCGGCGTGCAGAGCACCATCTCCCTGTGGAAGAACGGCATGGTCGATCCCGAAACGGCGATC
GCCCAGGCCGCCAATGCCGACGAACACCAGGCCGCGCTGGTCTTCCGCAACTATGTGGCTACGCTGAACGCCTACCAGGC
AGTCGATTTCGACGACCTGATCCGCTTGCCCGCCGAACTGTTCGCACGCAATGAAACGGTACAGCTCAAGTGGCAGAACC
GCCTGCGCTACTTCCTGGTCGACGAATACCAGGACACCAACGCGTGCCAGTACCAGCTGCTCAAGCTGCTGGCGGGCGGT
TCGCACCTGCGCGCGCCGGCATTCACCGCAGTGGGGGACGACGACCAGGCCATCTACGGCTGGCGCGGCGCCACGCTCGA
CAACCTGCGCCTGCTGCAGACCGATTTCCCCGACCTGAAGGTCGTCAAGCTCGAACAGAACTACCGTTCCACGGTGCGCA
TCCTGGAAGCGGCTAACGCGGTCATCGCCAACAACCCGAAGCTGTTCGACAAGAAGCTGTGGAGCGAGCACGGCATGGGC
GACCCGATCGCCATCAATCCGATGAACGACGAAGAGCACGAAGCCGAGTCGGTCGTGTTCCGCCTGTCCGCGCACAAGTT
TGAACGCCGGGCGCAGTTCCGCGATTACGCCATCCTGTACCGCGGCAACCACCAGGCACGGCTGTTCGAGCAGATCCTGC
GCCGCGAGCGCATTCCATATGTGCTGTCGGGCGGCCAGAGCTTCTTCGACAAGGCCGAGATCAAGGACATCTGCGCCTAT
CTTCGGCTGATCGCCAACCCGGACGACGATCCCGCATTCATCCGCGCGATCACCACGCCGCGCCGCGGCATCGGCAACAC
CACGCTGGAAGTGCTGGGCACGTTCGCGGGTCAAGCGAAGGTTTCGTTGTTCGAGGCCGCGATGATGGGCGGCATCGAAG
CCAAGCTGCAACCACGCCAGCTCGAGCCGCTGCGCGTGTTCTGCGAATCGATGGTGCGGCTGGCCGATCGCGCGGCGAAA
GACCCGGCCACCGTGGTGCTCGATGACCTGATGGAAGGCATCCACTACGAAGCCTATCTCTACGACGCCTTCGACGAACG
GCAGGCGCAGTCGCGCTGGACCAATACGCTCGAATTCCTGGACTGGCTCAAGCGCAAGGGCACCAAGCCCGAGGCGGAAG
CCGAGGGCGAGGCGGAAGCCGAGGGCGAGGCAACCGGCTTCGACAATGCCGACGGCTTTGGCAACGAAGGCAAGAACCTG
CTCGAACTCACCCAGACCGTGGCGCTGATGAGCATGCTCGAAGGCCGCGAGGAAGATCCCGATGCCGTGCGCCTGTCGAC
GCTGCATGCGTCGAAGGGGCTCGAGTATCCCCACGTGTTCCTGGTCGGCGTGGAAGAGGGCATCCTGCCCCATTGCCGCG
AGGACGAGGACATGGGCGACGAGAAGATCGAAGAAGAACGGCGACTGATGTACGTGGGAATTACGCGTGCGCAGCGCAGT
CTGCATATCAGCTGGTGCAAGAAGCGCAAGCGCGCGCGGGAAACCTACTCGTGCGAACCGTCGCGGTTCATCGGCGAGAT
GAAGCTGGAAGAGGCGCCGGCGCCGAAGGACGACATGCCTGCGATGAGTCCGAAGGATCGGCTCGGTGCGCTGAAGGCGT
TGCTCGGGAATGGCGGCAAGGCGGCGCAGGGCTGA

Upstream 100 bases:

>100_bases
AGCGTGTTAGCATGGTCAGCTTTGCGTCAGACGCCAGCCTGTTGCGGCATGTTGCGTTCCCGGCGCCTGCCCCTCTCCAC
GCCTACTTCCTTCCCCGCGC

Downstream 100 bases:

>100_bases
CGCCGGCAGGCAGCATTCAAGATACAAAGCGCTACCAGCGCGACATGACCCCGGCGGCGGGTAGCGCTGCGGACCTCGGG
GCCTCGCCGGCTTGGTTTCA

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MTHGLNAAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFEPRHIAAVTFTNKAAKEMQERIGKLMEGKTTRE
GKRLPLKQLTVCTFHSLGVQILRMEAEHVGLKPQFSIMDSDDCFGLIQEQLGTTDKKLIRGVQSTISLWKNGMVDPETAI
AQAANADEHQAALVFRNYVATLNAYQAVDFDDLIRLPAELFARNETVQLKWQNRLRYFLVDEYQDTNACQYQLLKLLAGG
SHLRAPAFTAVGDDDQAIYGWRGATLDNLRLLQTDFPDLKVVKLEQNYRSTVRILEAANAVIANNPKLFDKKLWSEHGMG
DPIAINPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAY
LRLIANPDDDPAFIRAITTPRRGIGNTTLEVLGTFAGQAKVSLFEAAMMGGIEAKLQPRQLEPLRVFCESMVRLADRAAK
DPATVVLDDLMEGIHYEAYLYDAFDERQAQSRWTNTLEFLDWLKRKGTKPEAEAEGEAEAEGEATGFDNADGFGNEGKNL
LELTQTVALMSMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHCREDEDMGDEKIEEERRLMYVGITRAQRS
LHISWCKKRKRARETYSCEPSRFIGEMKLEEAPAPKDDMPAMSPKDRLGALKALLGNGGKAAQG

Sequences:

>Translated_704_residues
MTHGLNAAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFEPRHIAAVTFTNKAAKEMQERIGKLMEGKTTRE
GKRLPLKQLTVCTFHSLGVQILRMEAEHVGLKPQFSIMDSDDCFGLIQEQLGTTDKKLIRGVQSTISLWKNGMVDPETAI
AQAANADEHQAALVFRNYVATLNAYQAVDFDDLIRLPAELFARNETVQLKWQNRLRYFLVDEYQDTNACQYQLLKLLAGG
SHLRAPAFTAVGDDDQAIYGWRGATLDNLRLLQTDFPDLKVVKLEQNYRSTVRILEAANAVIANNPKLFDKKLWSEHGMG
DPIAINPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAY
LRLIANPDDDPAFIRAITTPRRGIGNTTLEVLGTFAGQAKVSLFEAAMMGGIEAKLQPRQLEPLRVFCESMVRLADRAAK
DPATVVLDDLMEGIHYEAYLYDAFDERQAQSRWTNTLEFLDWLKRKGTKPEAEAEGEAEAEGEATGFDNADGFGNEGKNL
LELTQTVALMSMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHCREDEDMGDEKIEEERRLMYVGITRAQRS
LHISWCKKRKRARETYSCEPSRFIGEMKLEEAPAPKDDMPAMSPKDRLGALKALLGNGGKAAQG
>Mature_703_residues
THGLNAAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFEPRHIAAVTFTNKAAKEMQERIGKLMEGKTTREG
KRLPLKQLTVCTFHSLGVQILRMEAEHVGLKPQFSIMDSDDCFGLIQEQLGTTDKKLIRGVQSTISLWKNGMVDPETAIA
QAANADEHQAALVFRNYVATLNAYQAVDFDDLIRLPAELFARNETVQLKWQNRLRYFLVDEYQDTNACQYQLLKLLAGGS
HLRAPAFTAVGDDDQAIYGWRGATLDNLRLLQTDFPDLKVVKLEQNYRSTVRILEAANAVIANNPKLFDKKLWSEHGMGD
PIAINPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAYL
RLIANPDDDPAFIRAITTPRRGIGNTTLEVLGTFAGQAKVSLFEAAMMGGIEAKLQPRQLEPLRVFCESMVRLADRAAKD
PATVVLDDLMEGIHYEAYLYDAFDERQAQSRWTNTLEFLDWLKRKGTKPEAEAEGEAEAEGEATGFDNADGFGNEGKNLL
ELTQTVALMSMLEGREEDPDAVRLSTLHASKGLEYPHVFLVGVEEGILPHCREDEDMGDEKIEEERRLMYVGITRAQRSL
HISWCKKRKRARETYSCEPSRFIGEMKLEEAPAPKDDMPAMSPKDRLGALKALLGNGGKAAQG

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=684, Percent_Identity=46.7836257309941, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI2367296, Length=678, Percent_Identity=35.8407079646018, Blast_Score=355, Evalue=6e-99,
Organism=Saccharomyces cerevisiae, GI6322369, Length=722, Percent_Identity=28.1163434903047, Blast_Score=199, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78996; Mature: 78865

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHGLNAAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFEPRHIAAVTFTNK
CCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCH
AAKEMQERIGKLMEGKTTREGKRLPLKQLTVCTFHSLGVQILRMEAEHVGLKPQFSIMDS
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHEEEHHHHCCCCCCEEECCC
DDCFGLIQEQLGTTDKKLIRGVQSTISLWKNGMVDPETAIAQAANADEHQAALVFRNYVA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
TLNAYQAVDFDDLIRLPAELFARNETVQLKWQNRLRYFLVDEYQDTNACQYQLLKLLAGG
HHHHHHCCCHHHHHHCCHHHHCCCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCC
SHLRAPAFTAVGDDDQAIYGWRGATLDNLRLLQTDFPDLKVVKLEQNYRSTVRILEAANA
CCCCCCEEEEECCCCCEEEEECCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHCH
VIANNPKLFDKKLWSEHGMGDPIAINPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILY
HCCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
RGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANPDDDPAFIRAITTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
RRGIGNTTLEVLGTFAGQAKVSLFEAAMMGGIEAKLQPRQLEPLRVFCESMVRLADRAAK
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DPATVVLDDLMEGIHYEAYLYDAFDERQAQSRWTNTLEFLDWLKRKGTKPEAEAEGEAEA
CCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EGEATGFDNADGFGNEGKNLLELTQTVALMSMLEGREEDPDAVRLSTLHASKGLEYPHVF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCCCCEEE
LVGVEEGILPHCREDEDMGDEKIEEERRLMYVGITRAQRSLHISWCKKRKRARETYSCEP
EEECCCCCCCCCCCCCCCCHHHHHHHHCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCH
SRFIGEMKLEEAPAPKDDMPAMSPKDRLGALKALLGNGGKAAQG
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
THGLNAAQSEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEDKGFEPRHIAAVTFTNK
CCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCH
AAKEMQERIGKLMEGKTTREGKRLPLKQLTVCTFHSLGVQILRMEAEHVGLKPQFSIMDS
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCHHHEEEHHHHCCCCCCEEECCC
DDCFGLIQEQLGTTDKKLIRGVQSTISLWKNGMVDPETAIAQAANADEHQAALVFRNYVA
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHH
TLNAYQAVDFDDLIRLPAELFARNETVQLKWQNRLRYFLVDEYQDTNACQYQLLKLLAGG
HHHHHHCCCHHHHHHCCHHHHCCCCEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCC
SHLRAPAFTAVGDDDQAIYGWRGATLDNLRLLQTDFPDLKVVKLEQNYRSTVRILEAANA
CCCCCCEEEEECCCCCEEEEECCCCCCCEEEEECCCCCEEEEEECHHHHHHHHHHHHHCH
VIANNPKLFDKKLWSEHGMGDPIAINPMNDEEHEAESVVFRLSAHKFERRAQFRDYAILY
HCCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
RGNHQARLFEQILRRERIPYVLSGGQSFFDKAEIKDICAYLRLIANPDDDPAFIRAITTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
RRGIGNTTLEVLGTFAGQAKVSLFEAAMMGGIEAKLQPRQLEPLRVFCESMVRLADRAAK
CCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
DPATVVLDDLMEGIHYEAYLYDAFDERQAQSRWTNTLEFLDWLKRKGTKPEAEAEGEAEA
CCHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
EGEATGFDNADGFGNEGKNLLELTQTVALMSMLEGREEDPDAVRLSTLHASKGLEYPHVF
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHCCCCCCEEE
LVGVEEGILPHCREDEDMGDEKIEEERRLMYVGITRAQRSLHISWCKKRKRARETYSCEP
EEECCCCCCCCCCCCCCCCHHHHHHHHCEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCH
SRFIGEMKLEEAPAPKDDMPAMSPKDRLGALKALLGNGGKAAQG
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]