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The map label for this gene is speE2 [H]

Identifier: 73542987

GI number: 73542987

Start: 3612918

End: 3614429

Strand: Direct

Name: speE2 [H]

Synonym: Reut_A3304

Alternate gene names: 73542987

Gene position: 3612918-3614429 (Clockwise)

Preceding gene: 73542979

Following gene: 73542988

Centisome position: 94.91

GC content: 64.35

Gene sequence:

>1512_bases
ATGCGCGACCGTACCCTCGTTATTTCGGTGTTAATCGTGGCCTCCTGCGGGCTTGGCTACGAACTGATCGCCGGCGCCCT
GTCCAGCTATCTGCTGGGCGATTCCATCCTCCAGTTCTCGTCCATCATCGGCTGCTATCTGTTCGCCATGGGCGTAGGGT
CGTGGCTGTCGCGCTATGTGAAGGACGAGGACGTGCTCGCCCGCTTCATCGACATCGAGATACTGGTCGGCCTGCTCGGC
GGCATCTCGGCGGCGCTGCTGTTCGTGGTGTTCGCGTGGCTGTCGGCGCCGTTCCGCACGGCACTGTACGCCCTGGTGTT
CCTGACCGGCGTGCTCGTGGGCATGGAGATCCCGCTGGTCATGCGTATCCTGAACGCGCGCCGTGCGGCCTTCAGCGATC
TGGTGAGCCGTGTGTTGACATTCGACTATCTGGGCGCGCTGGCGGTGTCGCTGGTCTTCCCGCTGGTGCTCGCTCCAAGG
CTTGGTCTGTCGCGCACCGGCTTCCTGTTCGGCATGCTCAATGCGGCGGTGGCGCTGTACACCACGCGTCTGTTTCGCGA
CGAGCTGCCGCAGGTGCCGCTGCGCACGATGCGCGCGTTGGTGGTGATCGCGCTGCTTGGTGCTGGCTTTGCCGGGTCGG
ACCGGCTCACGCACTGGGCCGAACGCGGCCTGTTCGGCGACGAGATTATCCACAGTGAATCGACGCCATACCAGCGGCTG
GTGATCACGCGCTGGAAGGACGACCTGCGCCTGTACATCAACGGCAACCTGCAGTTCTCGTCACGCGACGAGCACCGCTA
TCACGAGGCCCTTGTGCATCCGGTAATGCAAGCGCTGCCGTGGGCGCGCCGCGTGCTGGTGATCGGCGGCGGCGATGGGC
TCGCTCTGCGCGAGATCCTGCGACACCGCAATATCGAGCATGTGACACTGGTCGATCTCGATCCGGCCATGACCACGCTG
TTCTCGCAGAGCGCCCCGCTGCGCGCGCTCAACCGGGACTCGCTGCGCGATGCGCGCGTGACGGTGTTCAACGCCGATGC
CGCGCAATGGATGGAGCAGAACGCCGAGGTATTCGACGCCATCATCGTCGACCTGCCCGACCCGTCCAACTTCGGTCTGG
GCAAGCTGTATTCGGTGCCGATGTACCGGCTGCTGTCGCGCCATCTGGCCGAGAAGGGTTATGCGGTGGTGCAGTCGACT
TCGCCGTACTACGCGCCGCGCTCGTTCTGGGGCATCGATGCAACGTTGCACGAGGCCGGCCTGCATACGTGGCCCTACCA
TGCCTATGTGCCGTCGTTCGGGGAATGGGGTTTCATCCTCGCCGGCAAACGCAGCGACTACACGCCGCCGACCCGCTATG
ACATGCCGATGAAATTCCTGGATGCGGACAGCACCGCGCTGATGTTCCGCTTCCCGCCCGACATGGCGCCGCGCGCGGGC
CATGCAAACCGCCTCAACGAGCAATCGCTCGTGCATGACTTCGAGCAGGACTGGGGCAATGTCATTCGCTGA

Upstream 100 bases:

>100_bases
ACGATTGCGCCGGCAGCGCGCGAATGCGCCGCAAGTATATCCGCAGTCTCATCCACCAGTTCCCCCCGAACCGTTCAGAA
CCCCGCCGGACTGCCGCCTG

Downstream 100 bases:

>100_bases
TACGCCGACGAAAGCACAGTGATGGATCGCCGCACGTTCCTGATCGGCGCGTCCGCCGCGCTGGCCGGCTGCGACCGGCT
TGGCGATGCGTCGCGCCGCT

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase 2; PAPT 2; SPDSY 2 [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MRDRTLVISVLIVASCGLGYELIAGALSSYLLGDSILQFSSIIGCYLFAMGVGSWLSRYVKDEDVLARFIDIEILVGLLG
GISAALLFVVFAWLSAPFRTALYALVFLTGVLVGMEIPLVMRILNARRAAFSDLVSRVLTFDYLGALAVSLVFPLVLAPR
LGLSRTGFLFGMLNAAVALYTTRLFRDELPQVPLRTMRALVVIALLGAGFAGSDRLTHWAERGLFGDEIIHSESTPYQRL
VITRWKDDLRLYINGNLQFSSRDEHRYHEALVHPVMQALPWARRVLVIGGGDGLALREILRHRNIEHVTLVDLDPAMTTL
FSQSAPLRALNRDSLRDARVTVFNADAAQWMEQNAEVFDAIIVDLPDPSNFGLGKLYSVPMYRLLSRHLAEKGYAVVQST
SPYYAPRSFWGIDATLHEAGLHTWPYHAYVPSFGEWGFILAGKRSDYTPPTRYDMPMKFLDADSTALMFRFPPDMAPRAG
HANRLNEQSLVHDFEQDWGNVIR

Sequences:

>Translated_503_residues
MRDRTLVISVLIVASCGLGYELIAGALSSYLLGDSILQFSSIIGCYLFAMGVGSWLSRYVKDEDVLARFIDIEILVGLLG
GISAALLFVVFAWLSAPFRTALYALVFLTGVLVGMEIPLVMRILNARRAAFSDLVSRVLTFDYLGALAVSLVFPLVLAPR
LGLSRTGFLFGMLNAAVALYTTRLFRDELPQVPLRTMRALVVIALLGAGFAGSDRLTHWAERGLFGDEIIHSESTPYQRL
VITRWKDDLRLYINGNLQFSSRDEHRYHEALVHPVMQALPWARRVLVIGGGDGLALREILRHRNIEHVTLVDLDPAMTTL
FSQSAPLRALNRDSLRDARVTVFNADAAQWMEQNAEVFDAIIVDLPDPSNFGLGKLYSVPMYRLLSRHLAEKGYAVVQST
SPYYAPRSFWGIDATLHEAGLHTWPYHAYVPSFGEWGFILAGKRSDYTPPTRYDMPMKFLDADSTALMFRFPPDMAPRAG
HANRLNEQSLVHDFEQDWGNVIR
>Mature_503_residues
MRDRTLVISVLIVASCGLGYELIAGALSSYLLGDSILQFSSIIGCYLFAMGVGSWLSRYVKDEDVLARFIDIEILVGLLG
GISAALLFVVFAWLSAPFRTALYALVFLTGVLVGMEIPLVMRILNARRAAFSDLVSRVLTFDYLGALAVSLVFPLVLAPR
LGLSRTGFLFGMLNAAVALYTTRLFRDELPQVPLRTMRALVVIALLGAGFAGSDRLTHWAERGLFGDEIIHSESTPYQRL
VITRWKDDLRLYINGNLQFSSRDEHRYHEALVHPVMQALPWARRVLVIGGGDGLALREILRHRNIEHVTLVDLDPAMTTL
FSQSAPLRALNRDSLRDARVTVFNADAAQWMEQNAEVFDAIIVDLPDPSNFGLGKLYSVPMYRLLSRHLAEKGYAVVQST
SPYYAPRSFWGIDATLHEAGLHTWPYHAYVPSFGEWGFILAGKRSDYTPPTRYDMPMKFLDADSTALMFRFPPDMAPRAG
HANRLNEQSLVHDFEQDWGNVIR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=249, Percent_Identity=26.9076305220884, Blast_Score=96, Evalue=8e-20,
Organism=Escherichia coli, GI1786312, Length=175, Percent_Identity=34.2857142857143, Blast_Score=99, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI212645616, Length=267, Percent_Identity=27.7153558052434, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25154106, Length=215, Percent_Identity=28.8372093023256, Blast_Score=69, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6325326, Length=255, Percent_Identity=27.843137254902, Blast_Score=93, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6323175, Length=235, Percent_Identity=26.8085106382979, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24645443, Length=237, Percent_Identity=28.2700421940928, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 56260; Mature: 56260

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDRTLVISVLIVASCGLGYELIAGALSSYLLGDSILQFSSIIGCYLFAMGVGSWLSRYV
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KDEDVLARFIDIEILVGLLGGISAALLFVVFAWLSAPFRTALYALVFLTGVLVGMEIPLV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
MRILNARRAAFSDLVSRVLTFDYLGALAVSLVFPLVLAPRLGLSRTGFLFGMLNAAVALY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
TTRLFRDELPQVPLRTMRALVVIALLGAGFAGSDRLTHWAERGLFGDEIIHSESTPYQRL
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHCCCCCCHHHH
VITRWKDDLRLYINGNLQFSSRDEHRYHEALVHPVMQALPWARRVLVIGGGDGLALREIL
EEEEECCCEEEEEECCEEECCCHHHHHHHHHHHHHHHHCCHHCEEEEEECCCCHHHHHHH
RHRNIEHVTLVDLDPAMTTLFSQSAPLRALNRDSLRDARVTVFNADAAQWMEQNAEVFDA
HHCCCCEEEEEECCHHHHHHHCCCCCCHHCCCCCCCCCEEEEEECHHHHHHHHCHHEEHH
IIVDLPDPSNFGLGKLYSVPMYRLLSRHLAEKGYAVVQSTSPYYAPRSFWGIDATLHEAG
HEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCHHHHHCC
LHTWPYHAYVPSFGEWGFILAGKRSDYTPPTRYDMPMKFLDADSTALMFRFPPDMAPRAG
CCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHEECCCCCEEEEECCCCCCCCCC
HANRLNEQSLVHDFEQDWGNVIR
CCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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CCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]