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The map label for this gene is pyk [H]

Identifier: 73542978

GI number: 73542978

Start: 3604367

End: 3605830

Strand: Direct

Name: pyk [H]

Synonym: Reut_A3295

Alternate gene names: 73542978

Gene position: 3604367-3605830 (Clockwise)

Preceding gene: 73542977

Following gene: 73542979

Centisome position: 94.69

GC content: 65.71

Gene sequence:

>1464_bases
ATGAGACGCCAGCGCAAAGCCAAGATCGTTGCCACGCTCGGTCCCGCGAGCACCGATATCGCGGTGATCCGACAGCTGTT
CGAAGCCGGGGCCGACGTATTCCGCCTGAACTTCAGCCATGGCACGCATGAAGACCACCGCGCGCGCTACGACGCTGTGC
GCCAGATCGAAGCCGAGACCGGCCGCCCGATCGCGGTGCTGGCCGACCTGCAGGGTCCGAAGCTGCGCATCGGCGTATTC
GCCGCCGGCAAGGTTGCCGTGCGCGCGGGCGACTCGTTCGTGCTCGACAGCGATCCGACCCCTGGCGATGGCACGCGCGT
CCATCTGCCGCATCCGGAACTGTTCCACGCAGCACAGCCGGGCCAGTCGCTGCTGATCGACGATGGCAAGGTGCGCCTTG
CGATCGAATCCGTGGCGAGCGGCAGCATCACCACGCGTGTGGTGAACAACGGCACGCTGTCGGACCGCAAGGGTGTAAAC
GTGCCTGATGCGGTCATCCCTATCCCTGCGCTGACCGAGAAGGACCGCAAGGACCTGGACTTTGCACTGTCGCTGGGTGC
TGACTGGATCGGCCTGTCGTTCGTGCAGCGCCCTTCCGACATCGTCGAAGCGCGCGAGATCGTCGGCACGCGTGCCGGCA
TCCTGTCGAAGATCGAAAAGCCCGCCGCGCTGCAGCAGCTGGAAGAGATCGTGCGTGTGTCGGATTCGGTGATGGTCGCG
CGTGGCGACCTTGGCGTCGAACTGCCGCCCGAGCGCGTGCCCGGCGTGCAAAAGCGCATCCTGCGTGTGTGCCGCCAGCT
GGGCAAGCCCGTGGTGATCGCTACGCAGATGCTTGAGTCGATGATTGACTCGCCGGTGCCGACGCGTGCTGAAGCCTCGG
ACGTGGCGAGTGCGATCTATGAAGGTGCGGATGCGGTGATGCTGTCGGCGGAGTCGGCCAATGGCCGCTACCCCGTGCCG
GCGGTGTCGATGATGAATCGCATCGTCACCGAGGTGGAGCGCGACCCGCTTTATCGCAACCTGCTCGATGCGCAGCATGA
GACGCCGCTGAACACCCGTCAGGATGCGATCTGCGCGGCGCTGCGGGAAGTGACGCAGATCATTGGTGCTGTGGCGACGG
TGACCTACACGTCGTCAGGTGCGACGGCGCTGCGCGCTGCGCGTGAACGGCCTTGCGCGCCGATCGTCAGCATCACGCCG
AACCTCGAGATCGCGCGCCGGCTGGCGATTGCCTGGGGCATTCACTCGACGGTGAGCCCCGACGTGAAGAGCGTTGACGA
GATGGTCGAAGCGGCAACGCGTGCGGCGCTGGTTGAAGGCTATGCCGCGCCGGGTGACCAGATCACGATTGCTGCTGGTA
TGCCGTTTGGGCAGGGCGGCACGACCAACTTGCTGCGCGTGGCGGAAGTCGGCGGCAATGCGGTGGCTGGTGTGGAATCT
GTGCGGGAGAGCGCCACAGTCTGA

Upstream 100 bases:

>100_bases
TTCCGCGCGATCCTTATCGTTTGAGTATCGCGTCCGAACTGAACCGCATTACCGGCGCGCCACCACGGCGCGCCGTTCCG
AGAATGGAAGAGGAGACATC

Downstream 100 bases:

>100_bases
TTTGTCTTGGTAGTTGGGCGCTGGCCGCGCCCGGATGGTGTTGATTGCCGCCCGTCACCCGCCAGTCTCATTGTTGAGGT
CTGGCGGGTGGCGTTTTTTG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MRRQRKAKIVATLGPASTDIAVIRQLFEAGADVFRLNFSHGTHEDHRARYDAVRQIEAETGRPIAVLADLQGPKLRIGVF
AAGKVAVRAGDSFVLDSDPTPGDGTRVHLPHPELFHAAQPGQSLLIDDGKVRLAIESVASGSITTRVVNNGTLSDRKGVN
VPDAVIPIPALTEKDRKDLDFALSLGADWIGLSFVQRPSDIVEAREIVGTRAGILSKIEKPAALQQLEEIVRVSDSVMVA
RGDLGVELPPERVPGVQKRILRVCRQLGKPVVIATQMLESMIDSPVPTRAEASDVASAIYEGADAVMLSAESANGRYPVP
AVSMMNRIVTEVERDPLYRNLLDAQHETPLNTRQDAICAALREVTQIIGAVATVTYTSSGATALRAARERPCAPIVSITP
NLEIARRLAIAWGIHSTVSPDVKSVDEMVEAATRAALVEGYAAPGDQITIAAGMPFGQGGTTNLLRVAEVGGNAVAGVES
VRESATV

Sequences:

>Translated_487_residues
MRRQRKAKIVATLGPASTDIAVIRQLFEAGADVFRLNFSHGTHEDHRARYDAVRQIEAETGRPIAVLADLQGPKLRIGVF
AAGKVAVRAGDSFVLDSDPTPGDGTRVHLPHPELFHAAQPGQSLLIDDGKVRLAIESVASGSITTRVVNNGTLSDRKGVN
VPDAVIPIPALTEKDRKDLDFALSLGADWIGLSFVQRPSDIVEAREIVGTRAGILSKIEKPAALQQLEEIVRVSDSVMVA
RGDLGVELPPERVPGVQKRILRVCRQLGKPVVIATQMLESMIDSPVPTRAEASDVASAIYEGADAVMLSAESANGRYPVP
AVSMMNRIVTEVERDPLYRNLLDAQHETPLNTRQDAICAALREVTQIIGAVATVTYTSSGATALRAARERPCAPIVSITP
NLEIARRLAIAWGIHSTVSPDVKSVDEMVEAATRAALVEGYAAPGDQITIAAGMPFGQGGTTNLLRVAEVGGNAVAGVES
VRESATV
>Mature_487_residues
MRRQRKAKIVATLGPASTDIAVIRQLFEAGADVFRLNFSHGTHEDHRARYDAVRQIEAETGRPIAVLADLQGPKLRIGVF
AAGKVAVRAGDSFVLDSDPTPGDGTRVHLPHPELFHAAQPGQSLLIDDGKVRLAIESVASGSITTRVVNNGTLSDRKGVN
VPDAVIPIPALTEKDRKDLDFALSLGADWIGLSFVQRPSDIVEAREIVGTRAGILSKIEKPAALQQLEEIVRVSDSVMVA
RGDLGVELPPERVPGVQKRILRVCRQLGKPVVIATQMLESMIDSPVPTRAEASDVASAIYEGADAVMLSAESANGRYPVP
AVSMMNRIVTEVERDPLYRNLLDAQHETPLNTRQDAICAALREVTQIIGAVATVTYTSSGATALRAARERPCAPIVSITP
NLEIARRLAIAWGIHSTVSPDVKSVDEMVEAATRAALVEGYAAPGDQITIAAGMPFGQGGTTNLLRVAEVGGNAVAGVES
VRESATV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=492, Percent_Identity=35.5691056910569, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI33286420, Length=492, Percent_Identity=35.5691056910569, Blast_Score=293, Evalue=2e-79,
Organism=Homo sapiens, GI10835121, Length=491, Percent_Identity=36.0488798370672, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI32967597, Length=491, Percent_Identity=36.0488798370672, Blast_Score=290, Evalue=2e-78,
Organism=Homo sapiens, GI33286418, Length=489, Percent_Identity=34.9693251533742, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI310128732, Length=287, Percent_Identity=34.4947735191638, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI310128730, Length=287, Percent_Identity=34.4947735191638, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI310128736, Length=230, Percent_Identity=31.7391304347826, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI310128734, Length=230, Percent_Identity=31.7391304347826, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI310128738, Length=209, Percent_Identity=31.5789473684211, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1788160, Length=484, Percent_Identity=38.6363636363636, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1787965, Length=469, Percent_Identity=36.0341151385927, Blast_Score=303, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=35.8762886597938, Blast_Score=303, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI71984413, Length=485, Percent_Identity=35.4639175257732, Blast_Score=269, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17506829, Length=485, Percent_Identity=35.4639175257732, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71984406, Length=485, Percent_Identity=35.4639175257732, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI17506831, Length=485, Percent_Identity=35.4639175257732, Blast_Score=268, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=32.4435318275154, Blast_Score=238, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6319279, Length=483, Percent_Identity=32.2981366459627, Blast_Score=234, Evalue=2e-62,
Organism=Drosophila melanogaster, GI24648964, Length=489, Percent_Identity=34.1513292433538, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI28571814, Length=489, Percent_Identity=34.1513292433538, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24648966, Length=417, Percent_Identity=36.9304556354916, Blast_Score=251, Evalue=7e-67,
Organism=Drosophila melanogaster, GI24581235, Length=494, Percent_Identity=32.3886639676113, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24646914, Length=240, Percent_Identity=38.75, Blast_Score=151, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51836; Mature: 51836

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRQRKAKIVATLGPASTDIAVIRQLFEAGADVFRLNFSHGTHEDHRARYDAVRQIEAET
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
GRPIAVLADLQGPKLRIGVFAAGKVAVRAGDSFVLDSDPTPGDGTRVHLPHPELFHAAQP
CCCEEEEEECCCCEEEEEEEECCEEEEEECCEEEECCCCCCCCCCEEECCCHHHHHCCCC
GQSLLIDDGKVRLAIESVASGSITTRVVNNGTLSDRKGVNVPDAVIPIPALTEKDRKDLD
CCEEEEECCEEEEEEEHHCCCCEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCH
FALSLGADWIGLSFVQRPSDIVEAREIVGTRAGILSKIEKPAALQQLEEIVRVSDSVMVA
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEE
RGDLGVELPPERVPGVQKRILRVCRQLGKPVVIATQMLESMIDSPVPTRAEASDVASAIY
ECCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
EGADAVMLSAESANGRYPVPAVSMMNRIVTEVERDPLYRNLLDAQHETPLNTRQDAICAA
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHH
LREVTQIIGAVATVTYTSSGATALRAARERPCAPIVSITPNLEIARRLAIAWGIHSTVSP
HHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCC
DVKSVDEMVEAATRAALVEGYAAPGDQITIAAGMPFGQGGTTNLLRVAEVGGNAVAGVES
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHEEEECCCCCHHHHHHH
VRESATV
HHHHCCC
>Mature Secondary Structure
MRRQRKAKIVATLGPASTDIAVIRQLFEAGADVFRLNFSHGTHEDHRARYDAVRQIEAET
CCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
GRPIAVLADLQGPKLRIGVFAAGKVAVRAGDSFVLDSDPTPGDGTRVHLPHPELFHAAQP
CCCEEEEEECCCCEEEEEEEECCEEEEEECCEEEECCCCCCCCCCEEECCCHHHHHCCCC
GQSLLIDDGKVRLAIESVASGSITTRVVNNGTLSDRKGVNVPDAVIPIPALTEKDRKDLD
CCEEEEECCEEEEEEEHHCCCCEEEEEECCCCCCCCCCCCCCCCEECCCCCCCCCCCCCH
FALSLGADWIGLSFVQRPSDIVEAREIVGTRAGILSKIEKPAALQQLEEIVRVSDSVMVA
HHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEE
RGDLGVELPPERVPGVQKRILRVCRQLGKPVVIATQMLESMIDSPVPTRAEASDVASAIY
ECCCCCCCCHHHCCCHHHHHHHHHHHHCCCEEHHHHHHHHHHCCCCCCCCCHHHHHHHHH
EGADAVMLSAESANGRYPVPAVSMMNRIVTEVERDPLYRNLLDAQHETPLNTRQDAICAA
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHH
LREVTQIIGAVATVTYTSSGATALRAARERPCAPIVSITPNLEIARRLAIAWGIHSTVSP
HHHHHHHHHHHHHEEECCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCC
DVKSVDEMVEAATRAALVEGYAAPGDQITIAAGMPFGQGGTTNLLRVAEVGGNAVAGVES
CHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHEEEECCCCCHHHHHHH
VRESATV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA