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The map label for this gene is dmlR [H]

Identifier: 73542969

GI number: 73542969

Start: 3593344

End: 3594255

Strand: Reverse

Name: dmlR [H]

Synonym: Reut_A3286

Alternate gene names: 73542969

Gene position: 3594255-3593344 (Counterclockwise)

Preceding gene: 73542970

Following gene: 73542968

Centisome position: 94.42

GC content: 65.46

Gene sequence:

>912_bases
ATGGACAAACTCAAGCAAATCGAAGCCTTTATCGCGGTGGTCGAGCACGGCAGCATGGCCGCTGCCGCGCTCACGCAGGA
CGTCACGCCCGTGATGATCGGGCGCCGCATCAACGCGCTGGAAGCGCGAATCGGCGTCAAGCTGCTGCATCGCTCGACAC
GCCGCATCGTGGTCACCGAGCAGGGCGCCGTATTCATGGAGCAGTGCAAGAAGGCGCTGGCCGACCTGGACCGCGCCGAG
ATGCTGATCGCCGAAGGCAAGCACAAGGCGACGGGCCACCTGATCGTGTCGGCCCCGGCCGCGTTCGGCCGCAAGCACGT
GGCGCCGCATGCGCCGGCCTTCCTGGCCGCGAACCCGGAAGTGCAGATTTCGTTCAACCTGACCGACCGCGTGGTCGATC
TCGTGCGCGAAGGGTATGACGTCGGCATCCGCATCGGCGGCGCGATCGACCCGAACTTCGTCGCCATCAAGCTGGCCACC
AACAAGCGCGTGGTGTGCGGTACGCCCGCGTACTTTGCCAAGCACGGCGTGCCGCTGACGCTGGACGACCTGGAGCACCA
CAACTGCCTGGCCTTCAACCTGCAGGGCGGCCAGCAGCGCGGCTGGTATTTCCAGCAGAACGGCAAGACCGTGACCGTGC
GCGTCAATGGCAATCTCGACTGCAACGATGGCGAATTGCTGCACCGCTGGACCGGCGAGCACCTGGGCCTCGGCTGGCGC
TCCACGTGGGAGATCTTGCCGCAACTGGAGAGCGGCGAATTGGTCACCGTGCTCGACGAGTACGCATTGCCGGATTACGA
CATCCTCGCCGTGTACCCGCAGCAGCGTCCGGTGCCGGCCAAGATCCGCTTCTTCATCGAGCATCTGAAAGCGCAATTCG
CGCAGCCGGGCTACTGGACGGCGCGCGGCTGA

Upstream 100 bases:

>100_bases
CTGGCCACCATGCGCAAGTCCCGATCCGCTTCATAAGTGCCGGCACGATGTCACGGCGCCGCCGATGCCGCACAATGGCG
GGCAGGAGACGCAACACCAT

Downstream 100 bases:

>100_bases
CCCGCGCAAGGGGAGGGCGCTGATGGCGAGGTGGCTGGCGGACCTGGTGGTCGTGGTCCACTTCCTGTTCATCGCCTTCG
TGGTCGGCGGCGCGCTGCTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MDKLKQIEAFIAVVEHGSMAAAALTQDVTPVMIGRRINALEARIGVKLLHRSTRRIVVTEQGAVFMEQCKKALADLDRAE
MLIAEGKHKATGHLIVSAPAAFGRKHVAPHAPAFLAANPEVQISFNLTDRVVDLVREGYDVGIRIGGAIDPNFVAIKLAT
NKRVVCGTPAYFAKHGVPLTLDDLEHHNCLAFNLQGGQQRGWYFQQNGKTVTVRVNGNLDCNDGELLHRWTGEHLGLGWR
STWEILPQLESGELVTVLDEYALPDYDILAVYPQQRPVPAKIRFFIEHLKAQFAQPGYWTARG

Sequences:

>Translated_303_residues
MDKLKQIEAFIAVVEHGSMAAAALTQDVTPVMIGRRINALEARIGVKLLHRSTRRIVVTEQGAVFMEQCKKALADLDRAE
MLIAEGKHKATGHLIVSAPAAFGRKHVAPHAPAFLAANPEVQISFNLTDRVVDLVREGYDVGIRIGGAIDPNFVAIKLAT
NKRVVCGTPAYFAKHGVPLTLDDLEHHNCLAFNLQGGQQRGWYFQQNGKTVTVRVNGNLDCNDGELLHRWTGEHLGLGWR
STWEILPQLESGELVTVLDEYALPDYDILAVYPQQRPVPAKIRFFIEHLKAQFAQPGYWTARG
>Mature_303_residues
MDKLKQIEAFIAVVEHGSMAAAALTQDVTPVMIGRRINALEARIGVKLLHRSTRRIVVTEQGAVFMEQCKKALADLDRAE
MLIAEGKHKATGHLIVSAPAAFGRKHVAPHAPAFLAANPEVQISFNLTDRVVDLVREGYDVGIRIGGAIDPNFVAIKLAT
NKRVVCGTPAYFAKHGVPLTLDDLEHHNCLAFNLQGGQQRGWYFQQNGKTVTVRVNGNLDCNDGELLHRWTGEHLGLGWR
STWEILPQLESGELVTVLDEYALPDYDILAVYPQQRPVPAKIRFFIEHLKAQFAQPGYWTARG

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=292, Percent_Identity=35.6164383561644, Blast_Score=183, Evalue=1e-47,
Organism=Escherichia coli, GI1789440, Length=291, Percent_Identity=34.3642611683849, Blast_Score=177, Evalue=7e-46,
Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=29.020979020979, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787589, Length=297, Percent_Identity=25.9259259259259, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=27.3972602739726, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789639, Length=297, Percent_Identity=25.2525252525253, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1787128, Length=292, Percent_Identity=25.6849315068493, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790011, Length=293, Percent_Identity=20.8191126279863, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=22.5589225589226, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=161, Percent_Identity=29.8136645962733, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1789173, Length=207, Percent_Identity=26.0869565217391, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33570; Mature: 33570

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKLKQIEAFIAVVEHGSMAAAALTQDVTPVMIGRRINALEARIGVKLLHRSTRRIVVTE
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEE
QGAVFMEQCKKALADLDRAEMLIAEGKHKATGHLIVSAPAAFGRKHVAPHAPAFLAANPE
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCHHHCCCCCCCCCCEEEECCCC
VQISFNLTDRVVDLVREGYDVGIRIGGAIDPNFVAIKLATNKRVVCGTPAYFAKHGVPLT
EEEEECCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCCHHHHHCCCCEE
LDDLEHHNCLAFNLQGGQQRGWYFQQNGKTVTVRVNGNLDCNDGELLHRWTGEHLGLGWR
ECCCCCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCC
STWEILPQLESGELVTVLDEYALPDYDILAVYPQQRPVPAKIRFFIEHLKAQFAQPGYWT
CHHHHCCCCCCCCEEEEEHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEE
ARG
CCC
>Mature Secondary Structure
MDKLKQIEAFIAVVEHGSMAAAALTQDVTPVMIGRRINALEARIGVKLLHRSTRRIVVTE
CCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEE
QGAVFMEQCKKALADLDRAEMLIAEGKHKATGHLIVSAPAAFGRKHVAPHAPAFLAANPE
CCHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEECCHHHCCCCCCCCCCEEEECCCC
VQISFNLTDRVVDLVREGYDVGIRIGGAIDPNFVAIKLATNKRVVCGTPAYFAKHGVPLT
EEEEECCHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEECCCEEEECCCHHHHHCCCCEE
LDDLEHHNCLAFNLQGGQQRGWYFQQNGKTVTVRVNGNLDCNDGELLHRWTGEHLGLGWR
ECCCCCCCEEEEEECCCCCCCEEEECCCCEEEEEECCCCCCCCCCEEEEECCCCCCCCCC
STWEILPQLESGELVTVLDEYALPDYDILAVYPQQRPVPAKIRFFIEHLKAQFAQPGYWT
CHHHHCCCCCCCCEEEEEHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCEEE
ARG
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]