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The map label for this gene is paaE [H]

Identifier: 73542894

GI number: 73542894

Start: 3522664

End: 3523752

Strand: Direct

Name: paaE [H]

Synonym: Reut_A3210

Alternate gene names: 73542894

Gene position: 3522664-3523752 (Clockwise)

Preceding gene: 73542893

Following gene: 73542895

Centisome position: 92.54

GC content: 67.22

Gene sequence:

>1089_bases
ATGACCCCACAGTTCCATCCGCTGCGCGTGGCGCAGGTGCGCCCCGAGACGGCCGACACGATTTCCATCGCGTTCGAAGT
GCCCGAGACCCTGCGCGACGCCTACCGCTTCACACAGGGGCAGTTCCTGACGCTGAAGGCCCCGGTCGATGGCCAGGACC
TGCGCCGCTCGTACTCGATCTGCTGCGGCGTGCAGGACTATGCCGAGCGCGGTGAGCTGCGCGTGGCAGTAAAACTCGTC
GAGGATGGCGTGTTTTCGAGCCACCTCCATGACACGCTGGCCCCGGGCCAGCTTATCGATGTGATGACGCCGGACGGCCG
CTTCCATGTGCCGCTCGACGCGGGCGCGGCACGCCACTATGTCGCCTTTGCCGCGGGCAGCGGCATCACGCCGGTGCTGT
CGCTGATCCGCACCGCGCTGGCGGCCGAACCGCAGAGCCGCTTCACGCTGGTCTATGGCAACCGCAATGTCGACAGCATC
ATTTTTTCCGAAGCGCTCGAAGACCTGAAGAACCAGTACCTGTCGCGCTTCACGCTGTACCACGTGCTGTCGCGCCAGCC
GCAGGAAGTCGACCTGCTGCACGGGCGCCTCGACCACGCCCGGGTCACGGCCTTCCTGCAGACGCTGATCCCGGTGGACG
GCATCGATGCGGCGTTCGTGTGCGGACCGGCCTCGATGATCGACGAGGTCGAAACCGCGCTGCGCGATGCCGGGTTGGAT
CCGCACCGCATCCACGCCGAGCGGTTCGGTGTGCCGGTGGACCGCCAAAAGCGCGCGCCCGCCCACGCCCATGCCGACGA
CGCCGGCACGGCCGAGCTGGTCGTGGTGCTCGATGGCAAGCAGCACACCATGCGCCTGCCGATGGAGGACGGCAAAGTGC
TCGACACGGCGCTGGCCGCCGGGCTGGACCTGCCCTATGCGTGCAAGGGCGGCGTGTGTTGCACCTGCCGGGCCAAGGTG
CTGGAAGGCCAGGTCGAGATGGAAAAGAACTACACGCTGGAGCCGTGGGAAATGGAAAAGGGCTTCGTCCTGACCTGCCA
GGCGCGCGCGCTCACGCCCCGCGTGGTGGTCAGCTACGACGAACGCTGA

Upstream 100 bases:

>100_bases
GCCTGCAAGGCGCTTTACCGTTGCCTCGATTGCCGCGAACCGTTCGACTACTTCAAACCCTACTAACCGTTACCAACCCC
GCCGTCCCGAGCGAGCTGCC

Downstream 100 bases:

>100_bases
GCCGCCGCGCCAAACGTGCGCCGGGCCGCGCGCGGTGTCTGGCACGCCCGGCTGCCCAAGTCCCGCCGTGATTACAATGG
GAAGGGAGCGCGTTGCGGAC

Product: phenylacetate-CoA oxygenase/reductase, PaaK subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MTPQFHPLRVAQVRPETADTISIAFEVPETLRDAYRFTQGQFLTLKAPVDGQDLRRSYSICCGVQDYAERGELRVAVKLV
EDGVFSSHLHDTLAPGQLIDVMTPDGRFHVPLDAGAARHYVAFAAGSGITPVLSLIRTALAAEPQSRFTLVYGNRNVDSI
IFSEALEDLKNQYLSRFTLYHVLSRQPQEVDLLHGRLDHARVTAFLQTLIPVDGIDAAFVCGPASMIDEVETALRDAGLD
PHRIHAERFGVPVDRQKRAPAHAHADDAGTAELVVVLDGKQHTMRLPMEDGKVLDTALAAGLDLPYACKGGVCCTCRAKV
LEGQVEMEKNYTLEPWEMEKGFVLTCQARALTPRVVVSYDER

Sequences:

>Translated_362_residues
MTPQFHPLRVAQVRPETADTISIAFEVPETLRDAYRFTQGQFLTLKAPVDGQDLRRSYSICCGVQDYAERGELRVAVKLV
EDGVFSSHLHDTLAPGQLIDVMTPDGRFHVPLDAGAARHYVAFAAGSGITPVLSLIRTALAAEPQSRFTLVYGNRNVDSI
IFSEALEDLKNQYLSRFTLYHVLSRQPQEVDLLHGRLDHARVTAFLQTLIPVDGIDAAFVCGPASMIDEVETALRDAGLD
PHRIHAERFGVPVDRQKRAPAHAHADDAGTAELVVVLDGKQHTMRLPMEDGKVLDTALAAGLDLPYACKGGVCCTCRAKV
LEGQVEMEKNYTLEPWEMEKGFVLTCQARALTPRVVVSYDER
>Mature_361_residues
TPQFHPLRVAQVRPETADTISIAFEVPETLRDAYRFTQGQFLTLKAPVDGQDLRRSYSICCGVQDYAERGELRVAVKLVE
DGVFSSHLHDTLAPGQLIDVMTPDGRFHVPLDAGAARHYVAFAAGSGITPVLSLIRTALAAEPQSRFTLVYGNRNVDSII
FSEALEDLKNQYLSRFTLYHVLSRQPQEVDLLHGRLDHARVTAFLQTLIPVDGIDAAFVCGPASMIDEVETALRDAGLDP
HRIHAERFGVPVDRQKRAPAHAHADDAGTAELVVVLDGKQHTMRLPMEDGKVLDTALAAGLDLPYACKGGVCCTCRAKVL
EGQVEMEKNYTLEPWEMEKGFVLTCQARALTPRVVVSYDER

Specific function: May be part of a multicomponent oxygenase involved in phenylacetyl-CoA hydroxylation [H]

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=358, Percent_Identity=46.927374301676, Blast_Score=319, Evalue=2e-88,
Organism=Escherichia coli, GI1788104, Length=361, Percent_Identity=24.6537396121884, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788903, Length=185, Percent_Identity=28.1081081081081, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR001709
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR011884
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39932; Mature: 39801

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPQFHPLRVAQVRPETADTISIAFEVPETLRDAYRFTQGQFLTLKAPVDGQDLRRSYSI
CCCCCCCEEEEEECCCCCCEEEEEEECCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
CCGVQDYAERGELRVAVKLVEDGVFSSHLHDTLAPGQLIDVMTPDGRFHVPLDAGAARHY
EECCHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCEE
VAFAAGSGITPVLSLIRTALAAEPQSRFTLVYGNRNVDSIIFSEALEDLKNQYLSRFTLY
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
HVLSRQPQEVDLLHGRLDHARVTAFLQTLIPVDGIDAAFVCGPASMIDEVETALRDAGLD
HHHCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCC
PHRIHAERFGVPVDRQKRAPAHAHADDAGTAELVVVLDGKQHTMRLPMEDGKVLDTALAA
HHEEHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHH
GLDLPYACKGGVCCTCRAKVLEGQVEMEKNYTLEPWEMEKGFVLTCQARALTPRVVVSYD
CCCCCCCCCCCEEEEEEHHHHCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEC
ER
CC
>Mature Secondary Structure 
TPQFHPLRVAQVRPETADTISIAFEVPETLRDAYRFTQGQFLTLKAPVDGQDLRRSYSI
CCCCCCEEEEEECCCCCCEEEEEEECCHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHH
CCGVQDYAERGELRVAVKLVEDGVFSSHLHDTLAPGQLIDVMTPDGRFHVPLDAGAARHY
EECCHHHHCCCCEEEEEEEECCCCHHHHHHHHCCCCCEEEEECCCCCEEEEECCCCCCEE
VAFAAGSGITPVLSLIRTALAAEPQSRFTLVYGNRNVDSIIFSEALEDLKNQYLSRFTLY
EEEECCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
HVLSRQPQEVDLLHGRLDHARVTAFLQTLIPVDGIDAAFVCGPASMIDEVETALRDAGLD
HHHCCCCCHHHHHHCCCHHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCC
PHRIHAERFGVPVDRQKRAPAHAHADDAGTAELVVVLDGKQHTMRLPMEDGKVLDTALAA
HHEEHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCCCCHHHHHHHH
GLDLPYACKGGVCCTCRAKVLEGQVEMEKNYTLEPWEMEKGFVLTCQARALTPRVVVSYD
CCCCCCCCCCCEEEEEEHHHHCCEEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEC
ER
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]