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The map label for this gene is pilD [H]

Identifier: 73542646

GI number: 73542646

Start: 3248441

End: 3249328

Strand: Direct

Name: pilD [H]

Synonym: Reut_A2962

Alternate gene names: 73542646

Gene position: 3248441-3249328 (Clockwise)

Preceding gene: 73542645

Following gene: 73542647

Centisome position: 85.34

GC content: 68.58

Gene sequence:

>888_bases
ATGAATCCGGCATTCCCCGCCGGCGGCGCCTTTGCGCTGCCGGCGCTGGCGGCACTGCCGCCGTCGTTCCTGGTGGCGGC
AGCCGCCGTGCTCGGCCTGCTGGTCGGCAGCTTCCTGAACGTGGTGATCCACCGGCTGCCGCGCATGATGGAACACGACG
AGGCCAACTACATCGCCGAAGTGCGCAGCGACCCGCTGCCCTACCCCGACCGCTACAACCTGATGGTGCCGCGCTCGGCC
TGCCCCCACTGCGGCCATGCGATCGCGGCCTGGGAGAATATTCCGGTTATCAGCTACCTGTTCCTGCGCGGACGCTGCTC
GTCATGCAAGGCACCGATCGGCATGCGCTATCCGCTAGTGGAGCTGGCGACCGGCGTGCTCAGCGCGCTGGCCGCTTGGC
ACTTCGGCGCGACCTGGCAGGGCGTGGCCGCGCTGGTGCTGCTGTGGGCGCTGATGGCACTGACGATGATCGACGCCGAT
ACGCAGTTGCTGCCCGACCAGATCACGCTGCCGCTGGTGTGGCTGGGCCTGCTGCTGAACCTGGCCGGGCTGTTCGTGCC
GATCGCCGATGCCGTGATCGGCGCCGTGGCCGGCTACCTGCTGCTGTGGAGCGCCTACTGGCTGTTCAAGCTCGTGCGCG
GCAAGGAAGGCATGGGCTACGGCGATTTCAAGCTGATGGCCGCGCTGGGCGCCTGGTTCGGCTGGCAGGCCTTGCCGTCG
CTGGTGCTGCTGTCGTCGGTGGTCGGCGTGGTGTTCGGCGTCGCCAATATCGCGCTGCGCCGCCAGGGACGGGACACGCC
GTTCCCGTTCGGCCCATTCATTGCGGGCGCCGGCGGGCTGGTGCTGTTCTTCGGCCCGGGTGTGCTGCCGCTGCTATTCA
TGCCGTAA

Upstream 100 bases:

>100_bases
GATCGAGCCGCTCATCATCGTGGTGCTGGGCGTGCTCATCGGCGGTATGGTGGTGGCAATGTACCTGCCGATCTTCAAGC
TGGGACAGGTGGTGTAATTG

Downstream 100 bases:

>100_bases
GCGGCGCCAGAGGACCGGAGAAGCGCCAAGCTGGTATCGTTTGGGGCATTGCACCCACGGAACCTGACATGCTGGAGATC
GGACTGACTGGCGGTATCGG

Product: prepilin peptidase

Products: NA

Alternate protein names: Protein pilD; Protein secretion protein XCPA; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MNPAFPAGGAFALPALAALPPSFLVAAAAVLGLLVGSFLNVVIHRLPRMMEHDEANYIAEVRSDPLPYPDRYNLMVPRSA
CPHCGHAIAAWENIPVISYLFLRGRCSSCKAPIGMRYPLVELATGVLSALAAWHFGATWQGVAALVLLWALMALTMIDAD
TQLLPDQITLPLVWLGLLLNLAGLFVPIADAVIGAVAGYLLLWSAYWLFKLVRGKEGMGYGDFKLMAALGAWFGWQALPS
LVLLSSVVGVVFGVANIALRRQGRDTPFPFGPFIAGAGGLVLFFGPGVLPLLFMP

Sequences:

>Translated_295_residues
MNPAFPAGGAFALPALAALPPSFLVAAAAVLGLLVGSFLNVVIHRLPRMMEHDEANYIAEVRSDPLPYPDRYNLMVPRSA
CPHCGHAIAAWENIPVISYLFLRGRCSSCKAPIGMRYPLVELATGVLSALAAWHFGATWQGVAALVLLWALMALTMIDAD
TQLLPDQITLPLVWLGLLLNLAGLFVPIADAVIGAVAGYLLLWSAYWLFKLVRGKEGMGYGDFKLMAALGAWFGWQALPS
LVLLSSVVGVVFGVANIALRRQGRDTPFPFGPFIAGAGGLVLFFGPGVLPLLFMP
>Mature_295_residues
MNPAFPAGGAFALPALAALPPSFLVAAAAVLGLLVGSFLNVVIHRLPRMMEHDEANYIAEVRSDPLPYPDRYNLMVPRSA
CPHCGHAIAAWENIPVISYLFLRGRCSSCKAPIGMRYPLVELATGVLSALAAWHFGATWQGVAALVLLWALMALTMIDAD
TQLLPDQITLPLVWLGLLLNLAGLFVPIADAVIGAVAGYLLLWSAYWLFKLVRGKEGMGYGDFKLMAALGAWFGWQALPS
LVLLSSVVGVVFGVANIALRRQGRDTPFPFGPFIAGAGGLVLFFGPGVLPLLFMP

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Processes the pilin precursor during membrane translocation. Required for the assembly of type IV pili and for secretion of most proteins [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=265, Percent_Identity=38.1132075471698, Blast_Score=160, Evalue=9e-41,
Organism=Escherichia coli, GI1789732, Length=125, Percent_Identity=50.4, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31552; Mature: 31552

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPAFPAGGAFALPALAALPPSFLVAAAAVLGLLVGSFLNVVIHRLPRMMEHDEANYIAE
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRSDPLPYPDRYNLMVPRSACPHCGHAIAAWENIPVISYLFLRGRCSSCKAPIGMRYPLV
HHCCCCCCCCCCCEEECCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHH
ELATGVLSALAAWHFGATWQGVAALVLLWALMALTMIDADTQLLPDQITLPLVWLGLLLN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHH
LAGLFVPIADAVIGAVAGYLLLWSAYWLFKLVRGKEGMGYGDFKLMAALGAWFGWQALPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVLLSSVVGVVFGVANIALRRQGRDTPFPFGPFIAGAGGLVLFFGPGVLPLLFMP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEECCCHHHHHCCC
>Mature Secondary Structure
MNPAFPAGGAFALPALAALPPSFLVAAAAVLGLLVGSFLNVVIHRLPRMMEHDEANYIAE
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRSDPLPYPDRYNLMVPRSACPHCGHAIAAWENIPVISYLFLRGRCSSCKAPIGMRYPLV
HHCCCCCCCCCCCEEECCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHH
ELATGVLSALAAWHFGATWQGVAALVLLWALMALTMIDADTQLLPDQITLPLVWLGLLLN
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHH
LAGLFVPIADAVIGAVAGYLLLWSAYWLFKLVRGKEGMGYGDFKLMAALGAWFGWQALPS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LVLLSSVVGVVFGVANIALRRQGRDTPFPFGPFIAGAGGLVLFFGPGVLPLLFMP
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCEEEEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7905475 [H]