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The map label for this gene is cobA [H]

Identifier: 73542376

GI number: 73542376

Start: 2953509

End: 2954312

Strand: Reverse

Name: cobA [H]

Synonym: Reut_A2691

Alternate gene names: 73542376

Gene position: 2954312-2953509 (Counterclockwise)

Preceding gene: 73542377

Following gene: 73542368

Centisome position: 77.61

GC content: 69.15

Gene sequence:

>804_bases
ATGGACAAGGCAGGCAAATCGACCGGCAAGGTCTACCTGATCGGGGCGGGCCCCGGGGCGGCGGACCTCATTACGGTGCG
CGGTGCACGGTTGTTGGGCGAAGCCCAGGTCGTGCTGCATGACGCACTGGTGTCGCCCGAGATGCTGGCCTGGTGTCCGC
AGGCCAGGCTGGTGGAAGTGGGCAAGCGCTGCGGCAAGCGTTCGACGGCACAACTGTTCATCAACCGCCAGATCATCGAC
ATGGCGACGAAGTACGAGCGCGTGGTGCGTCTCAAGGGCGGCGATCCTATGCTGTTCGGCCGCGCCGATGAGGAACTCCA
GGCGCTGGAAGCGGCTGGTATCGCGTACGAGGTGGTGCCCGGCATTACCGCGGCACTCGCGGCCGCGTCGGCCATCGCCA
AGCCGCTGACCAAGCGCGGCGTGTCGCGCAGCGTGGCCTTCGCCACGCAGGCCAAGGCCGCGGATGGCACGGAAGTGCCG
GCATCCGCCGCGGACATCGAAGCAGATGTGCGCGCCGATACGCTCGTGTACTACATGGGCCGCGACCAGGCCGCGGCCAT
CGCCGCGCAGCTGATCGCCCACGGCAAGGCGCCGTCGACGCCGGCATGGGTGGTCGAGGCGGTCAGCACGCCCCACCAGC
GCAGCCATGCCTTTACGCTGCAGCAGATGGCGGCGGGCGAAGCAGCCGGCTGGATCGACCCGTCGCATCCGAGCCTGCTG
ATGATCGGCGCGGCGCTGGCGGCGCGTGCGACGGAGCCGGTGACGCGCGATGTAGCCGCGACGATCTCGGCAAAGGCGGC
CTGA

Upstream 100 bases:

>100_bases
TGCTGATCGACGAGGCAACCAACCACACGGTCGCCGCGGGCATGATCCGTGCATACTCCTGAGCATCAGGACACACGTCA
CGCGAGGCAAGGGGCAGATG

Downstream 100 bases:

>100_bases
AATCAGTTTATGCGGAACAGCGAAAGCCGGGCACATGCCCGGCTTTTTGCTATTCAGGTCTACTGGCGCAGCGCATCGAC
ACTGAAGCGTCCGCCACCCG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MDKAGKSTGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQARLVEVGKRCGKRSTAQLFINRQIID
MATKYERVVRLKGGDPMLFGRADEELQALEAAGIAYEVVPGITAALAAASAIAKPLTKRGVSRSVAFATQAKAADGTEVP
ASAADIEADVRADTLVYYMGRDQAAAIAAQLIAHGKAPSTPAWVVEAVSTPHQRSHAFTLQQMAAGEAAGWIDPSHPSLL
MIGAALAARATEPVTRDVAATISAKAA

Sequences:

>Translated_267_residues
MDKAGKSTGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQARLVEVGKRCGKRSTAQLFINRQIID
MATKYERVVRLKGGDPMLFGRADEELQALEAAGIAYEVVPGITAALAAASAIAKPLTKRGVSRSVAFATQAKAADGTEVP
ASAADIEADVRADTLVYYMGRDQAAAIAAQLIAHGKAPSTPAWVVEAVSTPHQRSHAFTLQQMAAGEAAGWIDPSHPSLL
MIGAALAARATEPVTRDVAATISAKAA
>Mature_267_residues
MDKAGKSTGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQARLVEVGKRCGKRSTAQLFINRQIID
MATKYERVVRLKGGDPMLFGRADEELQALEAAGIAYEVVPGITAALAAASAIAKPLTKRGVSRSVAFATQAKAADGTEVP
ASAADIEADVRADTLVYYMGRDQAAAIAAQLIAHGKAPSTPAWVVEAVSTPHQRSHAFTLQQMAAGEAAGWIDPSHPSLL
MIGAALAARATEPVTRDVAATISAKAA

Specific function: Catalyzes both methylations at C-2 and C-7 of uroporphyrinogen III leading to precorrin-1 and precorrin-2; their oxidative esterification gives respectively factor I octamethyl ester and sirohydrochlorin [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=242, Percent_Identity=41.3223140495868, Blast_Score=148, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6322922, Length=244, Percent_Identity=29.5081967213115, Blast_Score=93, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 27730; Mature: 27730

Theoretical pI: Translated: 8.68; Mature: 8.68

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKAGKSTGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQARLVEV
CCCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHH
GKRCGKRSTAQLFINRQIIDMATKYERVVRLKGGDPMLFGRADEELQALEAAGIAYEVVP
HHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCHHHHHHHHHHCCHHHHHH
GITAALAAASAIAKPLTKRGVSRSVAFATQAKAADGTEVPASAADIEADVRADTLVYYMG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCEEEEEEC
RDQAAAIAAQLIAHGKAPSTPAWVVEAVSTPHQRSHAFTLQQMAAGEAAGWIDPSHPSLL
CCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCE
MIGAALAARATEPVTRDVAATISAKAA
EHHHHHHHHCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MDKAGKSTGKVYLIGAGPGAADLITVRGARLLGEAQVVLHDALVSPEMLAWCPQARLVEV
CCCCCCCCCEEEEEECCCCCCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHH
GKRCGKRSTAQLFINRQIIDMATKYERVVRLKGGDPMLFGRADEELQALEAAGIAYEVVP
HHHHCCCCHHHHHHHHHHHHHHHHHHHEEEECCCCEEEECCCHHHHHHHHHHCCHHHHHH
GITAALAAASAIAKPLTKRGVSRSVAFATQAKAADGTEVPASAADIEADVRADTLVYYMG
HHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCEEEEEEC
RDQAAAIAAQLIAHGKAPSTPAWVVEAVSTPHQRSHAFTLQQMAAGEAAGWIDPSHPSLL
CCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCE
MIGAALAARATEPVTRDVAATISAKAA
EHHHHHHHHCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]