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The map label for this gene is nadE [H]

Identifier: 73542295

GI number: 73542295

Start: 2860244

End: 2862292

Strand: Reverse

Name: nadE [H]

Synonym: Reut_A2610

Alternate gene names: 73542295

Gene position: 2862292-2860244 (Counterclockwise)

Preceding gene: 73542297

Following gene: 73542291

Centisome position: 75.19

GC content: 64.67

Gene sequence:

>2049_bases
ATGAAGAATCCGTTTTTCAACCTGTATTCCCACGGCTTTGCGCGCGTGGCCGTGGGCGTGCCGGTCTGCCGTGTGGCCGA
TCCGGCCTTCAATGCCGCCGAAACGATCGCACTGGCCACGCAGGCCGCAAAGCAGGGCGCCGTGCTGGTCGCCTTTCCGG
AACTCGGCCTCTCTGCTTATACGTGCGACGACCTGTTCCATCAGCGTGCGCTGCTCGATGCCTGCGAAGCGGCGCTGGGC
ACGATTGTCGAGGCGTCGCGCAAGCTTTCCGCCGCGATGATCGTCGGCATGCCGCTGCGGGTCGAGCACCAGTTGTTCAA
CTGCGCCGTGGTGGTGGCCCGAGGCCGTGTGCTCGGCGTGGTGCCGAAGTCCTACCTGCCGAACTACTGGGAGTTCTACG
AGGCGCGCCAGTTCAGCGAAGCCGACAACGCCGCCACCGATTCCATCCGCCTGCTCGGCCAGGATGTGCCGTTCGGCGCC
GGGCTGCTGTTCGAGATCGAGGACATTCCGTTCTTCCGCTTCCATGCGGAGATCTGCGAAGACGTATGGGTGCCGATTCC
GCCGTCGTCGTTTGCGGCACTGGCCGGTGCGACGGTGCTGGTCAACCTGTCGGCATCAAACATCGTGATCGGCAAGTCAG
GCTACCGGCACCAGCTGGTGTCACAGCAATCGGCGCGTTGCCTGGCCGCCTACCTCTACACGTCGGCTGGCAAGGGCGAG
TCGTCGACCGATCTCGCCTGGGACGGGCAGGCGCTGATTTGCGAGAACGGCGAGCTGCTGGCCGAGTCGGAGCGCTTCGC
CGATACCTCGCACGTGTTATTTGCGGATGTGGACGTGGAACGCCTGTCCCGAGAACGCATGCATCAGGTGACGTTCGGCC
ACTCGGTGCGCCGGCACAAGGCCGAGGTCGACAAATTCCGCGTGGTCCGCTTCCCGCTCGAGGTGTCGCGCGACAAGGCG
CTGCCGCTCGAACGCAAGGTGGCGCGCTTCCCGTACGTGCCGGCCGATCCACGCCAGCGCGACGAGCGCTGCCACGAGGT
CTACAACATCCAGGTGCAGGCGCTGGTGCAGCGGCTGTCGGCCAGCAAAATTTCCAAGGTGGTGATCGGCGTGTCGGGCG
GGCTCGATTCCACACATGCGCTGCTGGTGTGCGCGAAGGCGATGGACCGCCTGGGGTTGCCGCGCTCGAACATCCTGGCC
TACACCATGCCGGGCTTTGCGACCAGCGACCGCACGTTGCGCCAGGCGCGCCAACTGATGGAGCTGGTCGGCTGCACCGC
GCGCGAGATTGATATCCGGCCGAGTTGCCTCGCGATGCTGAAGGACCTGGACCATCCGTATGCGCGCGGCGAGAAGGTCT
ATGACGTGACCTTCGAGAACGTGCAGGCCGGCGAGCGCACCAACCACCTGTTCCGGCTGGCCAATCACAACGGCGCCATT
GTGATCGGCACGGGCGACCTCAGCGAACTCGCGCTGGGCTGGTGCACCTACGGCGTGGGCGACCATATGTCGCACTACAA
CGTGAATGCGAGCGTGCCGAAGACGCTGATCTCGCACCTGGTGCGCTGGGTGGCCGAGACCGGGCAGGTCGGCGACAGCG
GCTCGGAGGTGCTGATCCATGTGCTCGACACGGATATCAGCCCGGAACTGGTGCCTGGCGATTCGAACCACGGGCCGGAG
CAGAAGACCGAAAGCACGATCGGCCCGTACGAGCTGCAGGACTTCAATCTCTACTACACGTTGCGGTTCGGCTTTGCGCC
GTCCAAGATCGCGTTTCTGTCACTGCACGCGTGGGGCGATCGCGAACGCGGCATCTGGCCGAACGGGCCGCATGTGGTGC
GCAACCAGTACGGTCTTCCGGACATCAAGCGCAATCTGGGGATATTCCTTGATCGCTTCTTCCGTACCAGCCAGTTCAAG
CGGTCCTGCATTCCGAATGCGCCGAAGGTGGGTTCGGGCGGGTCGCTGTCGCCACGGGGCGACTGGCGTGCGCCGAGCGA
CTCCGAATCGGTGGTGTGGCTGAAGGATCTGGAGAAGGTGCCTGACTGA

Upstream 100 bases:

>100_bases
GTAGAATGACGTATGGCCGTCACAGCAGCGGCCGCAGGAATCCCCCGATGCCGCCGGCTGCAAGCGCGTGCGATGCAGAC
GCCGGCAAACAAGGCCCGAT

Downstream 100 bases:

>100_bases
GCGATTGGACAGACTAGGCCGACGGCGGTGACGCAAAGCCGCCGGTCGCCAGATCTTTGCAAAAAGCCATGAGCCAGTCG
GCCGTTTCATCAAGCGGGTA

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 682; Mature: 682

Protein sequence:

>682_residues
MKNPFFNLYSHGFARVAVGVPVCRVADPAFNAAETIALATQAAKQGAVLVAFPELGLSAYTCDDLFHQRALLDACEAALG
TIVEASRKLSAAMIVGMPLRVEHQLFNCAVVVARGRVLGVVPKSYLPNYWEFYEARQFSEADNAATDSIRLLGQDVPFGA
GLLFEIEDIPFFRFHAEICEDVWVPIPPSSFAALAGATVLVNLSASNIVIGKSGYRHQLVSQQSARCLAAYLYTSAGKGE
SSTDLAWDGQALICENGELLAESERFADTSHVLFADVDVERLSRERMHQVTFGHSVRRHKAEVDKFRVVRFPLEVSRDKA
LPLERKVARFPYVPADPRQRDERCHEVYNIQVQALVQRLSASKISKVVIGVSGGLDSTHALLVCAKAMDRLGLPRSNILA
YTMPGFATSDRTLRQARQLMELVGCTAREIDIRPSCLAMLKDLDHPYARGEKVYDVTFENVQAGERTNHLFRLANHNGAI
VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLISHLVRWVAETGQVGDSGSEVLIHVLDTDISPELVPGDSNHGPE
QKTESTIGPYELQDFNLYYTLRFGFAPSKIAFLSLHAWGDRERGIWPNGPHVVRNQYGLPDIKRNLGIFLDRFFRTSQFK
RSCIPNAPKVGSGGSLSPRGDWRAPSDSESVVWLKDLEKVPD

Sequences:

>Translated_682_residues
MKNPFFNLYSHGFARVAVGVPVCRVADPAFNAAETIALATQAAKQGAVLVAFPELGLSAYTCDDLFHQRALLDACEAALG
TIVEASRKLSAAMIVGMPLRVEHQLFNCAVVVARGRVLGVVPKSYLPNYWEFYEARQFSEADNAATDSIRLLGQDVPFGA
GLLFEIEDIPFFRFHAEICEDVWVPIPPSSFAALAGATVLVNLSASNIVIGKSGYRHQLVSQQSARCLAAYLYTSAGKGE
SSTDLAWDGQALICENGELLAESERFADTSHVLFADVDVERLSRERMHQVTFGHSVRRHKAEVDKFRVVRFPLEVSRDKA
LPLERKVARFPYVPADPRQRDERCHEVYNIQVQALVQRLSASKISKVVIGVSGGLDSTHALLVCAKAMDRLGLPRSNILA
YTMPGFATSDRTLRQARQLMELVGCTAREIDIRPSCLAMLKDLDHPYARGEKVYDVTFENVQAGERTNHLFRLANHNGAI
VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLISHLVRWVAETGQVGDSGSEVLIHVLDTDISPELVPGDSNHGPE
QKTESTIGPYELQDFNLYYTLRFGFAPSKIAFLSLHAWGDRERGIWPNGPHVVRNQYGLPDIKRNLGIFLDRFFRTSQFK
RSCIPNAPKVGSGGSLSPRGDWRAPSDSESVVWLKDLEKVPD
>Mature_682_residues
MKNPFFNLYSHGFARVAVGVPVCRVADPAFNAAETIALATQAAKQGAVLVAFPELGLSAYTCDDLFHQRALLDACEAALG
TIVEASRKLSAAMIVGMPLRVEHQLFNCAVVVARGRVLGVVPKSYLPNYWEFYEARQFSEADNAATDSIRLLGQDVPFGA
GLLFEIEDIPFFRFHAEICEDVWVPIPPSSFAALAGATVLVNLSASNIVIGKSGYRHQLVSQQSARCLAAYLYTSAGKGE
SSTDLAWDGQALICENGELLAESERFADTSHVLFADVDVERLSRERMHQVTFGHSVRRHKAEVDKFRVVRFPLEVSRDKA
LPLERKVARFPYVPADPRQRDERCHEVYNIQVQALVQRLSASKISKVVIGVSGGLDSTHALLVCAKAMDRLGLPRSNILA
YTMPGFATSDRTLRQARQLMELVGCTAREIDIRPSCLAMLKDLDHPYARGEKVYDVTFENVQAGERTNHLFRLANHNGAI
VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLISHLVRWVAETGQVGDSGSEVLIHVLDTDISPELVPGDSNHGPE
QKTESTIGPYELQDFNLYYTLRFGFAPSKIAFLSLHAWGDRERGIWPNGPHVVRNQYGLPDIKRNLGIFLDRFFRTSQFK
RSCIPNAPKVGSGGSLSPRGDWRAPSDSESVVWLKDLEKVPD

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=523, Percent_Identity=23.5181644359465, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17538756, Length=275, Percent_Identity=29.8181818181818, Blast_Score=100, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6321865, Length=285, Percent_Identity=27.0175438596491, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24641841, Length=268, Percent_Identity=25.3731343283582, Blast_Score=77, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24641843, Length=268, Percent_Identity=25.3731343283582, Blast_Score=77, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 75280; Mature: 75280

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPFFNLYSHGFARVAVGVPVCRVADPAFNAAETIALATQAAKQGAVLVAFPELGLSAY
CCCCCHHHHHCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEE
TCDDLFHQRALLDACEAALGTIVEASRKLSAAMIVGMPLRVEHQLFNCAVVVARGRVLGV
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHEEEEEECCCEEEE
VPKSYLPNYWEFYEARQFSEADNAATDSIRLLGQDVPFGAGLLFEIEDIPFFRFHAEICE
CCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHH
DVWVPIPPSSFAALAGATVLVNLSASNIVIGKSGYRHQLVSQQSARCLAAYLYTSAGKGE
CCCCCCCCCHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
SSTDLAWDGQALICENGELLAESERFADTSHVLFADVDVERLSRERMHQVTFGHSVRRHK
CCCCEEECCCEEEECCCCEEECCHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AEVDKFRVVRFPLEVSRDKALPLERKVARFPYVPADPRQRDERCHEVYNIQVQALVQRLS
HHHCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASKISKVVIGVSGGLDSTHALLVCAKAMDRLGLPRSNILAYTMPGFATSDRTLRQARQLM
HHHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
ELVGCTAREIDIRPSCLAMLKDLDHPYARGEKVYDVTFENVQAGERTNHLFRLANHNGAI
HHHCCCCEEECCCHHHHHHHHHCCCHHHCCCEEEEEEECCCCCCCCCCCEEEEECCCCEE
VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLISHLVRWVAETGQVGDSGSEVLIH
EEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VLDTDISPELVPGDSNHGPEQKTESTIGPYELQDFNLYYTLRFGFAPSKIAFLSLHAWGD
EECCCCCCCEECCCCCCCCCHHHHCCCCCEEEEEEEEEEEEEECCCCCCEEEEEEEECCC
RERGIWPNGPHVVRNQYGLPDIKRNLGIFLDRFFRTSQFKRSCIPNAPKVGSGGSLSPRG
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
DWRAPSDSESVVWLKDLEKVPD
CCCCCCCCCCEEEHHHHHCCCC
>Mature Secondary Structure
MKNPFFNLYSHGFARVAVGVPVCRVADPAFNAAETIALATQAAKQGAVLVAFPELGLSAY
CCCCCHHHHHCCHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCEE
TCDDLFHQRALLDACEAALGTIVEASRKLSAAMIVGMPLRVEHQLFNCAVVVARGRVLGV
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHEEEEEECCCEEEE
VPKSYLPNYWEFYEARQFSEADNAATDSIRLLGQDVPFGAGLLFEIEDIPFFRFHAEICE
CCHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHH
DVWVPIPPSSFAALAGATVLVNLSASNIVIGKSGYRHQLVSQQSARCLAAYLYTSAGKGE
CCCCCCCCCHHHHHCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCC
SSTDLAWDGQALICENGELLAESERFADTSHVLFADVDVERLSRERMHQVTFGHSVRRHK
CCCCEEECCCEEEECCCCEEECCHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
AEVDKFRVVRFPLEVSRDKALPLERKVARFPYVPADPRQRDERCHEVYNIQVQALVQRLS
HHHCCEEEEEECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ASKISKVVIGVSGGLDSTHALLVCAKAMDRLGLPRSNILAYTMPGFATSDRTLRQARQLM
HHHHEEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHH
ELVGCTAREIDIRPSCLAMLKDLDHPYARGEKVYDVTFENVQAGERTNHLFRLANHNGAI
HHHCCCCEEECCCHHHHHHHHHCCCHHHCCCEEEEEEECCCCCCCCCCCEEEEECCCCEE
VIGTGDLSELALGWCTYGVGDHMSHYNVNASVPKTLISHLVRWVAETGQVGDSGSEVLIH
EEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEE
VLDTDISPELVPGDSNHGPEQKTESTIGPYELQDFNLYYTLRFGFAPSKIAFLSLHAWGD
EECCCCCCCEECCCCCCCCCHHHHCCCCCEEEEEEEEEEEEEECCCCCCEEEEEEEECCC
RERGIWPNGPHVVRNQYGLPDIKRNLGIFLDRFFRTSQFKRSCIPNAPKVGSGGSLSPRG
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
DWRAPSDSESVVWLKDLEKVPD
CCCCCCCCCCEEEHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]