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The map label for this gene is paaF [C]

Identifier: 73542239

GI number: 73542239

Start: 2798553

End: 2799356

Strand: Reverse

Name: paaF [C]

Synonym: Reut_A2554

Alternate gene names: 73542239

Gene position: 2799356-2798553 (Counterclockwise)

Preceding gene: 73542240

Following gene: 73542238

Centisome position: 73.54

GC content: 67.29

Gene sequence:

>804_bases
ATGGCCGACACCGATCCGCGCACGAGCGCACCCCTGCTGACCGAGTCGCGCGATGCCGCGGGCGTGGCGCGCCTGACCAT
GAACCGCCCGGAAGCGTTCAACGCGTTGTCCGAAGACCTGCTGGACGAACTGGCTGCCGCCTTCGCCCGCCTGGCCGCGG
ACGAGTCGGTACGCGTGGTGGTACTGGGCGGCGCCGGCAAGGCCTTCTGCGCCGGGCACGATTTGCGCGAGATGCGCGCC
TCGCCATCGCATGACTACTACCGGCGCCTGTTCGACCGCTGCACCCGGGTGATGATGGCCATCCAGAAGATGCCGCAGCC
GGTCATCGCGCGCGTACATGGCATTGCGACGGCGGCAGGCTGTCAGCTGGTGGCCATGTGCGACCTCGCCGTGGCTGCGG
ACGATGCGCGCTTTGCCGTATCGGGTGTCAATCTGGGGCTGTTCTGTTCGACGCCCGGCGTTGCCCTGTCACGCAACCTC
TCGCGCAAGCAGGCCATGGAGATGCTGCTGACCGGCGACATGATCGATGCTGCTACGGCCTGCGCGCGGGGGCTGGTCAA
CCGGATCGCGCCGCTGGCCCAGCTCGATGAAGAAGTGGCACGGCTGGCGGCCAGCATCTGCGCCAAGCCGGCGGCGGCCG
TGGCGGCAGGAAAGGGTCTGTTCTACAAGCAACTCGAAATGGGCATCGAGGCGGCCTACCAGCTCGCTGGACAGACTATG
GCCTGCAACATGATGGATGAGTCTGCGCTGGAGGGCGTCCAGGCCTTCATCGACAAGCGGGCGCCGAGTTGGCGTGCCTC
ATGA

Upstream 100 bases:

>100_bases
ATCGTCAGCTGTCTCGAGGGCGGCTACAACCTCAGCGCGCTGGGCCGCAGCGTGCTGGCACACCTGAAAGTGCTGCTGGA
ACGATAAGGAGCAAGCCCGC

Downstream 100 bases:

>100_bases
GGCCTATGAGCCGCAGCTATATGCCTTGAGTGCATAAAGCCATCGGAAAATATTCGTTTATTGCATGAGGCGGATCGCTT
AAAATGCTCGCTTCATAAAA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MADTDPRTSAPLLTESRDAAGVARLTMNRPEAFNALSEDLLDELAAAFARLAADESVRVVVLGGAGKAFCAGHDLREMRA
SPSHDYYRRLFDRCTRVMMAIQKMPQPVIARVHGIATAAGCQLVAMCDLAVAADDARFAVSGVNLGLFCSTPGVALSRNL
SRKQAMEMLLTGDMIDAATACARGLVNRIAPLAQLDEEVARLAASICAKPAAAVAAGKGLFYKQLEMGIEAAYQLAGQTM
ACNMMDESALEGVQAFIDKRAPSWRAS

Sequences:

>Translated_267_residues
MADTDPRTSAPLLTESRDAAGVARLTMNRPEAFNALSEDLLDELAAAFARLAADESVRVVVLGGAGKAFCAGHDLREMRA
SPSHDYYRRLFDRCTRVMMAIQKMPQPVIARVHGIATAAGCQLVAMCDLAVAADDARFAVSGVNLGLFCSTPGVALSRNL
SRKQAMEMLLTGDMIDAATACARGLVNRIAPLAQLDEEVARLAASICAKPAAAVAAGKGLFYKQLEMGIEAAYQLAGQTM
ACNMMDESALEGVQAFIDKRAPSWRAS
>Mature_266_residues
ADTDPRTSAPLLTESRDAAGVARLTMNRPEAFNALSEDLLDELAAAFARLAADESVRVVVLGGAGKAFCAGHDLREMRAS
PSHDYYRRLFDRCTRVMMAIQKMPQPVIARVHGIATAAGCQLVAMCDLAVAADDARFAVSGVNLGLFCSTPGVALSRNLS
RKQAMEMLLTGDMIDAATACARGLVNRIAPLAQLDEEVARLAASICAKPAAAVAAGKGLFYKQLEMGIEAAYQLAGQTMA
CNMMDESALEGVQAFIDKRAPSWRAS

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=43.4108527131783, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=34.2857142857143, Blast_Score=134, Evalue=9e-32,
Organism=Homo sapiens, GI70995211, Length=257, Percent_Identity=30.3501945525292, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI260274832, Length=256, Percent_Identity=23.828125, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI45643119, Length=219, Percent_Identity=26.4840182648402, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI260275230, Length=219, Percent_Identity=26.4840182648402, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI4502327, Length=252, Percent_Identity=26.5873015873016, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4757968, Length=211, Percent_Identity=25.5924170616114, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI49355787, Length=211, Percent_Identity=25.5924170616114, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4757966, Length=176, Percent_Identity=27.8409090909091, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI51538581, Length=176, Percent_Identity=27.8409090909091, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI25453481, Length=176, Percent_Identity=27.8409090909091, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI51538573, Length=176, Percent_Identity=27.8409090909091, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI221136756, Length=215, Percent_Identity=26.5116279069767, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221136753, Length=215, Percent_Identity=26.5116279069767, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221307494, Length=215, Percent_Identity=26.5116279069767, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI37594469, Length=199, Percent_Identity=26.6331658291457, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI37594471, Length=199, Percent_Identity=26.6331658291457, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=32.6530612244898, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=31.7460317460317, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=31.6205533596838, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI87082183, Length=256, Percent_Identity=28.125, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1787660, Length=266, Percent_Identity=29.3233082706767, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1788682, Length=187, Percent_Identity=28.3422459893048, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17540306, Length=246, Percent_Identity=39.4308943089431, Blast_Score=176, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI25145438, Length=266, Percent_Identity=34.5864661654135, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17554946, Length=247, Percent_Identity=30.7692307692308, Blast_Score=121, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560910, Length=260, Percent_Identity=27.6923076923077, Blast_Score=101, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17540714, Length=188, Percent_Identity=29.7872340425532, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=199, Percent_Identity=29.1457286432161, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17536985, Length=277, Percent_Identity=23.826714801444, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=214, Percent_Identity=27.5700934579439, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=210, Percent_Identity=27.1428571428571, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25144276, Length=209, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17508951, Length=197, Percent_Identity=27.9187817258883, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI19922422, Length=249, Percent_Identity=36.5461847389558, Blast_Score=183, Evalue=9e-47,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=31.1475409836066, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=31.1475409836066, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24653139, Length=264, Percent_Identity=26.8939393939394, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19920382, Length=208, Percent_Identity=29.8076923076923, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24650670, Length=275, Percent_Identity=26.1818181818182, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21357171, Length=210, Percent_Identity=28.0952380952381, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28342; Mature: 28211

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADTDPRTSAPLLTESRDAAGVARLTMNRPEAFNALSEDLLDELAAAFARLAADESVRVV
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VLGGAGKAFCAGHDLREMRASPSHDYYRRLFDRCTRVMMAIQKMPQPVIARVHGIATAAG
EECCCCCHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
CQLVAMCDLAVAADDARFAVSGVNLGLFCSTPGVALSRNLSRKQAMEMLLTGDMIDAATA
HHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCHHHHHCHHHHHHHHHHHHCCHHHHHHH
CARGLVNRIAPLAQLDEEVARLAASICAKPAAAVAAGKGLFYKQLEMGIEAAYQLAGQTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHH
ACNMMDESALEGVQAFIDKRAPSWRAS
HHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ADTDPRTSAPLLTESRDAAGVARLTMNRPEAFNALSEDLLDELAAAFARLAADESVRVV
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
VLGGAGKAFCAGHDLREMRASPSHDYYRRLFDRCTRVMMAIQKMPQPVIARVHGIATAAG
EECCCCCHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
CQLVAMCDLAVAADDARFAVSGVNLGLFCSTPGVALSRNLSRKQAMEMLLTGDMIDAATA
HHHHHHHHHHHHCCCCCEEEECCCEEEEECCCCHHHHHCHHHHHHHHHHHHCCHHHHHHH
CARGLVNRIAPLAQLDEEVARLAASICAKPAAAVAAGKGLFYKQLEMGIEAAYQLAGQTM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHH
ACNMMDESALEGVQAFIDKRAPSWRAS
HHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA