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The map label for this gene is yjbJ [H]

Identifier: 73542043

GI number: 73542043

Start: 2598151

End: 2598933

Strand: Reverse

Name: yjbJ [H]

Synonym: Reut_A2357

Alternate gene names: 73542043

Gene position: 2598933-2598151 (Counterclockwise)

Preceding gene: 73542044

Following gene: 73542042

Centisome position: 68.28

GC content: 64.5

Gene sequence:

>783_bases
ATGAGAACGAGACCGACCCGCAAACCATGGCAACAGGCGGCCGCAGCCGCGCTGATGGTGGCTGGTCTCGCCTGCGCGCC
GGCGGCGCATGCCGAACTCTACGGCTTCGTCGATGCCGATGGCATTGCTCACTTTGCAGACCGCAAGCTCGACGAGCGCT
ACAAGCTTTTCATGCGGGAAGGCGGCAGCTTCGATACTTCGCGCGAGTCGAGCGCCGCGCCAAAGAGCGACCTTGAGCAG
CACAAGCTGTACCGCTACGTCGTCAACCACCCGAATATCGCCAAGGTCGAGCCGATGATCCGGCAGATCGCGGACAAGCA
GGATGTCGACCCGGCGCTGGTCAAGGCCGTGATGGCCGTGGAGTCCGGCTTCAATCCATCGGCCGTATCGCCCAAGGGCG
CCATCGGGCTGATGCAGGTGATTCCCGATACCGGCGCGCGTTTCGGCGTGAGCGGCGATGCGCGCCGCAGCGTCGAGGAA
AAACTGGCCGATCCGCGTACCAATATCTCTGCCGGCGTGCGCTATCTGCGCTGGCTGATGGAACTGTTCCCCGACAACAT
CGAACTGGTACTGGCCGCCTACAACGCGGGCGAGGGCGCGGTGCAGCGCTACAACAACCGGATTCCGCCGTACCCGGAGA
CGCAGCAATACGTGAGCACCGTGCTGCAGTTCTACCGGCTCTACAAGCCCGGCGGCGGTGCGGTCGTGACCCGCACCAGC
GCGGCAGTTGGCGGGCCTTCACGCGTAAAGATGGTGATCGGCGGACGGCGCAACATGCCCTGA

Upstream 100 bases:

>100_bases
AACGAAGTCGAGGCGGACATCTGAGCGCCAGTCTGTACGCCGTCCGACATTGGGGGATAATGCGCGCTTCGACGTCCTCG
CCCGCAAGCCGCAACGCAAG

Downstream 100 bases:

>100_bases
CCGAGATACCCGAATAACAAGCCACCCGGCGCGCCGGCTTCACATCATGGCGCGCCGTCTTCTTGCCAATCCATGGAACA
ACAAGACATCACGTTTCAAC

Product: lytic transglycosylase, catalytic

Products: N-Acetylmuramic Acid Residues; N-Acetylglucosamine Residues [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MRTRPTRKPWQQAAAAALMVAGLACAPAAHAELYGFVDADGIAHFADRKLDERYKLFMREGGSFDTSRESSAAPKSDLEQ
HKLYRYVVNHPNIAKVEPMIRQIADKQDVDPALVKAVMAVESGFNPSAVSPKGAIGLMQVIPDTGARFGVSGDARRSVEE
KLADPRTNISAGVRYLRWLMELFPDNIELVLAAYNAGEGAVQRYNNRIPPYPETQQYVSTVLQFYRLYKPGGGAVVTRTS
AAVGGPSRVKMVIGGRRNMP

Sequences:

>Translated_260_residues
MRTRPTRKPWQQAAAAALMVAGLACAPAAHAELYGFVDADGIAHFADRKLDERYKLFMREGGSFDTSRESSAAPKSDLEQ
HKLYRYVVNHPNIAKVEPMIRQIADKQDVDPALVKAVMAVESGFNPSAVSPKGAIGLMQVIPDTGARFGVSGDARRSVEE
KLADPRTNISAGVRYLRWLMELFPDNIELVLAAYNAGEGAVQRYNNRIPPYPETQQYVSTVLQFYRLYKPGGGAVVTRTS
AAVGGPSRVKMVIGGRRNMP
>Mature_260_residues
MRTRPTRKPWQQAAAAALMVAGLACAPAAHAELYGFVDADGIAHFADRKLDERYKLFMREGGSFDTSRESSAAPKSDLEQ
HKLYRYVVNHPNIAKVEPMIRQIADKQDVDPALVKAVMAVESGFNPSAVSPKGAIGLMQVIPDTGARFGVSGDARRSVEE
KLADPRTNISAGVRYLRWLMELFPDNIELVLAAYNAGEGAVQRYNNRIPPYPETQQYVSTVLQFYRLYKPGGGAVVTRTS
AAVGGPSRVKMVIGGRRNMP

Specific function: Murein-Degrading Enzyme. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division (By Similarity). [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Attached To The Membrane By A Lipid Anchor [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082191, Length=117, Percent_Identity=40.1709401709402, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082441, Length=163, Percent_Identity=31.9018404907975, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 28480; Mature: 28480

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRPTRKPWQQAAAAALMVAGLACAPAAHAELYGFVDADGIAHFADRKLDERYKLFMRE
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHC
GGSFDTSRESSAAPKSDLEQHKLYRYVVNHPNIAKVEPMIRQIADKQDVDPALVKAVMAV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ESGFNPSAVSPKGAIGLMQVIPDTGARFGVSGDARRSVEEKLADPRTNISAGVRYLRWLM
HCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ELFPDNIELVLAAYNAGEGAVQRYNNRIPPYPETQQYVSTVLQFYRLYKPGGGAVVTRTS
HHCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
AAVGGPSRVKMVIGGRRNMP
CCCCCCHHEEEEECCCCCCC
>Mature Secondary Structure
MRTRPTRKPWQQAAAAALMVAGLACAPAAHAELYGFVDADGIAHFADRKLDERYKLFMRE
CCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHC
GGSFDTSRESSAAPKSDLEQHKLYRYVVNHPNIAKVEPMIRQIADKQDVDPALVKAVMAV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
ESGFNPSAVSPKGAIGLMQVIPDTGARFGVSGDARRSVEEKLADPRTNISAGVRYLRWLM
HCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ELFPDNIELVLAAYNAGEGAVQRYNNRIPPYPETQQYVSTVLQFYRLYKPGGGAVVTRTS
HHCCCCEEEEEEECCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECC
AAVGGPSRVKMVIGGRRNMP
CCCCCCHHEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]