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The map label for this gene is mdh [H]

Identifier: 73542013

GI number: 73542013

Start: 2565936

End: 2566919

Strand: Direct

Name: mdh [H]

Synonym: Reut_A2327

Alternate gene names: 73542013

Gene position: 2565936-2566919 (Clockwise)

Preceding gene: 73541983

Following gene: 73542014

Centisome position: 67.41

GC content: 63.92

Gene sequence:

>984_bases
ATGGCTAAAGCCCCAATGCGCGTCGCAGTGACCGGCGCCGCTGGCCAGATCGGCTACTCCCTGCTGTTCCGCATCGCCAA
CGGCGACATGCTGGGCAAAGACCAGCCGGTCATCCTCCAACTGCTCGACCTGCCCCAGGCCCAGGCGGCCGTCAAGGGCG
TGGTGATGGAACTGGAAGACTGCGCCTTCCCGCTGCTGGCCGGCGTGGTCATCACCGATGATCCGAAGGTTGCGTTCAAG
GACGCCGACGTTGCCCTGCTGGTGGGCGCCCGTCCGCGCAGCAAGGGCATGGAACGCAAGGACCTGCTCGAAGCCAACGC
CCAGATCTTCACGGTGCAGGGCAAGGCACTCGACGAAGTCGCCAGCCGCAACGTCAAGGTGCTGGTGGTCGGCAACCCGG
CCAACACCAACGCCTACATCGCCATGAAGTCGGCACCGAGCCTGCCGCGCGAAAACTTCACCGCCATGCTGCGCCTGGAC
CACAACCGTGCCCTGTCGCAGATCGCCGCCAAGACCGGCAAGCCGGTGGCCTCGATCGAGAAGATGTTCGTGTGGGGCAA
CCACAGCCCGACCATGTACGCCGACTATCGCTACGCGACTGTCGACGGCAAGAGCGTCAAGGACATGATCAACGATCCGG
TGTGGAACAACGACGTGTTCCTGCCGACCGTTGGCAAGCGCGGCGCCGCCATCATCGAAGCGCGTGGCCTGTCGTCGGCT
GCCTCGGCTGCCAACGCCGCCATCGACCACGTGCATGATTGGGTGCTGGGTACCAACGGCAAGGTCGTCACCATGGGCAT
CCCGTCGAACGGCGAGTACGGCATCCCGGCCGACACCATGTTCGGCTACCCCGTGACCTGCGCCAACGGCAAGTACGAAA
TCGTCAAGGGTCTGGAGATCGACGCCTACAGCCAGGAAAAGATCAACATCACCCTGAACGAACTGGAAGAAGAGAAGGCC
GGCGTGCAGCACCTGCTCGGCTGA

Upstream 100 bases:

>100_bases
GCTCCCCGCTGTGGCGACAACCAGCACGTGGTGACGAGCCTCCCCCTACGCGGCACGCCACCCACCGGTTTCCCCCATCT
ATTCGTTTGGAGATTTACTC

Downstream 100 bases:

>100_bases
TCCTCGGACCCTGTCCGCATAGGAAACCGGAGCACCGCAACGATTCACGAATCGGCGCGGGCTCCGGTTTTTTTGTATTG
GGCCCCTACCCTCGACTGTT

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELEDCAFPLLAGVVITDDPKVAFK
DADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEVASRNVKVLVVGNPANTNAYIAMKSAPSLPRENFTAMLRLD
HNRALSQIAAKTGKPVASIEKMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSA
ASAANAAIDHVHDWVLGTNGKVVTMGIPSNGEYGIPADTMFGYPVTCANGKYEIVKGLEIDAYSQEKINITLNELEEEKA
GVQHLLG

Sequences:

>Translated_327_residues
MAKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELEDCAFPLLAGVVITDDPKVAFK
DADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEVASRNVKVLVVGNPANTNAYIAMKSAPSLPRENFTAMLRLD
HNRALSQIAAKTGKPVASIEKMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSA
ASAANAAIDHVHDWVLGTNGKVVTMGIPSNGEYGIPADTMFGYPVTCANGKYEIVKGLEIDAYSQEKINITLNELEEEKA
GVQHLLG
>Mature_326_residues
AKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELEDCAFPLLAGVVITDDPKVAFKD
ADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEVASRNVKVLVVGNPANTNAYIAMKSAPSLPRENFTAMLRLDH
NRALSQIAAKTGKPVASIEKMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSAA
SAANAAIDHVHDWVLGTNGKVVTMGIPSNGEYGIPADTMFGYPVTCANGKYEIVKGLEIDAYSQEKINITLNELEEEKAG
VQHLLG

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=328, Percent_Identity=52.7439024390244, Blast_Score=338, Evalue=4e-93,
Organism=Homo sapiens, GI89886456, Length=335, Percent_Identity=24.4776119402985, Blast_Score=103, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17561064, Length=322, Percent_Identity=53.416149068323, Blast_Score=324, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI32566798, Length=260, Percent_Identity=55.3846153846154, Blast_Score=268, Evalue=4e-72,
Organism=Caenorhabditis elegans, GI32566800, Length=169, Percent_Identity=46.7455621301775, Blast_Score=147, Evalue=6e-36,
Organism=Drosophila melanogaster, GI24583394, Length=320, Percent_Identity=55.9375, Blast_Score=342, Evalue=1e-94,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 35022; Mature: 34890

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELED
CCCCCEEEEEECCCCHHCEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
CAFPLLAGVVITDDPKVAFKDADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEV
HHHHHHCCEEEECCCCEEEECCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCHHHHH
ASRNVKVLVVGNPANTNAYIAMKSAPSLPRENFTAMLRLDHNRALSQIAAKTGKPVASIE
HCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHEE
KMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSA
EEEEEECCCCCEEEEEEEEEECCHHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCCHH
ASAANAAIDHVHDWVLGTNGKVVTMGIPSNGEYGIPADTMFGYPVTCANGKYEIVKGLEI
HHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEECEEE
DAYSQEKINITLNELEEEKAGVQHLLG
CCCCCCEEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKAPMRVAVTGAAGQIGYSLLFRIANGDMLGKDQPVILQLLDLPQAQAAVKGVVMELED
CCCCEEEEEECCCCHHCEEEEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHH
CAFPLLAGVVITDDPKVAFKDADVALLVGARPRSKGMERKDLLEANAQIFTVQGKALDEV
HHHHHHCCEEEECCCCEEEECCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEECCHHHHH
ASRNVKVLVVGNPANTNAYIAMKSAPSLPRENFTAMLRLDHNRALSQIAAKTGKPVASIE
HCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHEE
KMFVWGNHSPTMYADYRYATVDGKSVKDMINDPVWNNDVFLPTVGKRGAAIIEARGLSSA
EEEEEECCCCCEEEEEEEEEECCHHHHHHHCCCCCCCCEEEECCCCCCCEEEEECCCCHH
ASAANAAIDHVHDWVLGTNGKVVTMGIPSNGEYGIPADTMFGYPVTCANGKYEIVKGLEI
HHHHHHHHHHHHHHEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEECEEE
DAYSQEKINITLNELEEEKAGVQHLLG
CCCCCCEEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA